Single liposome analysis of peptide translocation by the ABC transporter TAPL.

abstract：:Sarcoplasmic reticulum vesicles were exposed to various thiol-directed spin labels, and the position of the label on the inner or outer vesicle surface was investigated as a function of the ATPase (adenosinetriphosphatase; ATP phosphohydrolase, EC 3.6.1.3) chemical state. Previous measurements of label accessibility t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Champeil P,Rigaud JL,Gary-Bobo CM

更新日期：1980-05-01 00:00:00

abstract：:Barttin is an accessory subunit of a subgroup of ClC-type chloride channels expressed in renal and inner ear epithelia. In this study, we examined the effects of barttin on two ClC-K channel isoforms, rat ClC-K1 and human ClC-Kb, using heterologous expression, patch clamping, confocal imaging, and flow cytometry. In t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Scholl U,Hebeisen S,Janssen AG,Müller-Newen G,Alekov A,Fahlke C

更新日期：2006-07-25 00:00:00

abstract：:We measured quantitatively the binding affinities of purified Cro repressor to the chemically synthesized wild-type and mutant OR1 operators, consisting of all three possible base-pair substitutions and of thymine to uracil substitutions at each base-pair position of the 17-base-pair operator sequence. The sequence-sp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Takeda Y,Sarai A,Rivera VM

更新日期：1989-01-01 00:00:00

abstract：:Individuals with developmental dyslexia vary in their ability to improve reading skills, but the brain basis for improvement remains largely unknown. We performed a prospective, longitudinal study over 2.5 y in children with dyslexia (n = 25) or without dyslexia (n = 20) to discover whether initial behavioral or brain...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hoeft F,McCandliss BD,Black JM,Gantman A,Zakerani N,Hulme C,Lyytinen H,Whitfield-Gabrieli S,Glover GH,Reiss AL,Gabrieli JD

更新日期：2011-01-04 00:00:00

abstract：:To examine the regulation of midbrain dopamine neurons, recordings were obtained from single neurons of freely moving, genetically engineered dopamine-deficient (DD) mice. DD mice were tested without dopamine signaling (basal state) and with endogenous dopamine signaling (after L-dopa administration). In the basal sta...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Robinson S,Smith DM,Mizumori SJ,Palmiter RD

更新日期：2004-09-07 00:00:00

abstract：:On April 14, 2010, when meltwaters from the Eyjafjallajökull glacier mixed with hot magma, an explosive eruption sent unusually fine-grained ash into the jet stream. It quickly dispersed over Europe. Previous airplane encounters with ash resulted in sandblasted windows and particles melted inside jet engines, causing ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gislason SR,Hassenkam T,Nedel S,Bovet N,Eiriksdottir ES,Alfredsson HA,Hem CP,Balogh ZI,Dideriksen K,Oskarsson N,Sigfusson B,Larsen G,Stipp SL

更新日期：2011-05-03 00:00:00

abstract：:Artemis gene mutations are responsible for the development of a severe combined immunodeficiency [radiation-sensitive (RS) SCID] characterized by a severe B and T cell deficiency and a normal natural killer cell population. To establish the feasibility of a gene therapy approach to the treatment of RS-SCID, we generat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mostoslavsky G,Fabian AJ,Rooney S,Alt FW,Mulligan RC

更新日期：2006-10-31 00:00:00

abstract：:The suppressor of Hairy-wing [su(Hw)] protein exerts a polar effect on gene expression by repressing the function of transcriptional enhancers located distally from the promoter with respect to the location of su(Hw) binding sequences. The directionality of this effect suggests that the su(Hw) protein specifically int...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Georgiev PG,Corces VG

更新日期：1995-05-23 00:00:00

abstract：:Genetic selections that use proteinaceous transdominant inhibitors encoded by DNA libraries to cause mutant phenocopies may facilitate genetic analysis in traditionally nongenetic organisms. We performed a selection for random short peptides and larger protein fragments (collectively termed "perturbagens") that inhibi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Caponigro G,Abedi MR,Hurlburt AP,Maxfield A,Judd W,Kamb A

更新日期：1998-06-23 00:00:00

abstract：:Daytime sleepiness impairs cognitive ability, but recent evidence suggests it is also an important driver of human motivation and behavior. We aimed to investigate the relationship between sleepiness and a behavior strongly associated with better health: social activity. We additionally aimed to investigate whether a ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Holding BC,Sundelin T,Schiller H,Åkerstedt T,Kecklund G,Axelsson J

更新日期：2020-09-01 00:00:00

abstract：:Ribozymes are RNAs that possess the dual properties of RNA sequence-specific recognition, analogous to conventional antisense molecules, and RNA substrate destruction via site-specific cleavage. The cleavage reaction is catalytic in that more than one substrate molecule is processed per ribozyme molecule. We have desi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ojwang JO,Hampel A,Looney DJ,Wong-Staal F,Rappaport J

更新日期：1992-11-15 00:00:00

abstract：:Protein Hal is a human gamma4 heavy-chain disease protein whose molecular weight is reduced from 55,000 to 25,000 in 6 M guanidine due to the lack of disulfide bonds between heavy chains. Studies of aminoacid sequence indicate that it contains a gap of about 240 residues, starting 10 residues from the N-terminal end a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Frangione B,Lee L,Haber E,Bloch KJ

更新日期：1973-04-01 00:00:00

abstract：:Based on the DNA sequence of the symbiotic plasmid of Rhizobium strain NGR234, we predicted potential rearrangements generated by homologous recombination. All predicted rearrangements were identified experimentally by using a PCR-based methodology. Thus, the predicted and the actual dynamic maps of the replicon coinc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Flores M,Mavingui P,Perret X,Broughton WJ,Romero D,Hernández G,Dávila G,Palacios R

更新日期：2000-08-01 00:00:00

abstract：:During cardiac trabeculation, cardiomyocytes delaminate from the outermost (compact) layer to form complex muscular structures known as trabeculae. As these cardiomyocytes delaminate, the remodeling of adhesion junctions must be tightly coordinated so cells can extrude from the compact layer while remaining in tight c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cherian AV,Fukuda R,Augustine SM,Maischein HM,Stainier DY

更新日期：2016-07-05 00:00:00

abstract：:The strikingly high incidence of obstructed labor due to the disproportion of fetal size and the mother's pelvic dimensions has puzzled evolutionary scientists for decades. Here we propose that these high rates are a direct consequence of the distinct characteristics of human obstetric selection. Neonatal size relativ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mitteroecker P,Huttegger SM,Fischer B,Pavlicev M

更新日期：2016-12-20 00:00:00

abstract：:Three lines of evidence indicate that RNA-dependent RNA synthesis occurs in mouse erythroleukemia cells. The first involves labeling studies with [3H]uridine and shows a greater initial labeling rate of globin RNA in the cytoplasm than in the nucleus. Labeled globin RNA found in the cytoplasm after a very short pulse ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Volloch V

更新日期：1986-03-01 00:00:00

abstract：:The Cercospora nicotianae SOR1 (singlet oxygen resistance) gene was identified previously as a gene involved in resistance of this fungus to singlet-oxygen-generating phototoxins. Although homologues to SOR1 occur in organisms in four kingdoms and encode one of the most highly conserved proteins yet identified, the pr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ehrenshaft M,Bilski P,Li MY,Chignell CF,Daub ME

更新日期：1999-08-03 00:00:00

abstract：:Helodermin is a vasoactive intestinal peptide-like peptide in the salivary gland venom of the lizard Heloderma suspectum. Helodermin-like immunofluorescence was observed in the parafollicular (C) cells in several mammals and in the C cell homologues of the chicken ultimobranchial gland. Thus, helodermin-like peptides ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Grunditz T,Persson P,Håkanson R,Absood A,Böttcher G,Rerup C,Sundler F

更新日期：1989-02-01 00:00:00

abstract：:Chronic hypoxia causes pulmonary hypertension with smooth muscle cell proliferation and matrix deposition in the wall of the pulmonary arterioles. We demonstrate here that hypoxia also induces a pronounced inflammation in the lung before the structural changes of the vessel wall. The proinflammatory action of hypoxia ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Minamino T,Christou H,Hsieh CM,Liu Y,Dhawan V,Abraham NG,Perrella MA,Mitsialis SA,Kourembanas S

更新日期：2001-07-17 00:00:00

abstract：:Triglyceridemic response to a standard oral fat meal was determined in 28 healthy subjects and related to the levels of several lipids, lipoproteins, and apolipoproteins in the post-absorptive plasma. A fatty test meal was administered orally, and postprandial plasma triglyceride levels were determined. In the fasting...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Patsch JR,Karlin JB,Scott LW,Smith LC,Gotto AM Jr

更新日期：1983-03-01 00:00:00

abstract：:The antitumor effect of doxorubicin (DOX) conjugated to a biodegradable dendrimer was evaluated in mice bearing C-26 colon carcinomas. An asymmetric biodegradable polyester dendrimer containing 8-10 wt % DOX was prepared. The design of the dendrimer carrier optimized blood circulation time through size and molecular a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lee CC,Gillies ER,Fox ME,Guillaudeu SJ,Fréchet JM,Dy EE,Szoka FC

更新日期：2006-11-07 00:00:00

abstract：:Surfactant replacement therapy (SRT) involves instillation of a liquid-surfactant mixture directly into the lung airway tree. It is widely successful for treating surfactant deficiency in premature neonates who develop neonatal respiratory distress syndrome (NRDS). However, when applied to adults with acute respirator...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Filoche M,Tai CF,Grotberg JB

更新日期：2015-07-28 00:00:00

abstract：:The energetics of the TiO(2) polymorphs (rutile, anatase, and brookite) were studied by high temperature oxide melt drop solution calorimetry. Relative to bulk rutile, bulk brookite is 0.71 +/- 0.38 kJ/mol (6) and bulk anatase is 2.61 +/- 0.41 kJ/mol higher in enthalpy. The surface enthalpies of rutile, brookite, and ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ranade MR,Navrotsky A,Zhang HZ,Banfield JF,Elder SH,Zaban A,Borse PH,Kulkarni SK,Doran GS,Whitfield HJ

更新日期：2002-04-30 00:00:00

abstract：:Adaptive behavior requires animals to learn from experience. Ideally, learning should both promote choices that lead to rewards and reduce choices that lead to losses. Because the ventral striatum (VS) contains neurons that respond to aversive stimuli and aversive stimuli can drive dopamine release in the VS, it is po...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Taswell CA,Costa VD,Murray EA,Averbeck BB

更新日期：2018-12-26 00:00:00

abstract：:Quantifying the uncertainty for the present climate and the predictions of climate change in the suite of imperfect Atmosphere Ocean Science (AOS) computer models is a central issue in climate change science. Here, a systematic approach to these issues with firm mathematical underpinning is developed through empirical...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Majda AJ,Gershgorin B

更新日期：2010-08-24 00:00:00

abstract：:Both neocortical and hippocampal networks organize the firing patterns of their neurons by prominent oscillations during sleep, but the functional role of these rhythms is not well understood. Here, we show a robust correlation of neuronal discharges between the somatosensory cortex and hippocampus on both slow and fi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sirota A,Csicsvari J,Buhl D,Buzsáki G

更新日期：2003-02-18 00:00:00

abstract：:The first step in the formation of the protease resistant form (PrPSc) of prion proteins involves a conformational transition of the monomeric cellular form of PrPC to a more stable aggregation prone state PrPC*. A search of PDBselect and Escherichia coli and yeast genomes shows that the exact pattern of charges in he...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dima RI,Thirumalai D

更新日期：2004-10-26 00:00:00

abstract：:We previously have identified a subpopulation of contact-insensitive (CS-) cells which lacks density-dependent inhibition of cell division in primary and low-passage cultures of Syrian hamster embryonic (SHE) fibroblastic cells. Further, we have shown that the proportion of these CS- cells declines as a result of the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nakano S,Ueo H,Bruce SA,Ts'o PO

更新日期：1985-08-01 00:00:00

abstract：:Xenotropic and polytropic murine leukemia viruses (X-MLVs and P-MLVs) cross-interfere to various extents in non-mouse species and in wild Asian mice, suggesting that they might use a common receptor for infection. Consistent with this hypothesis, the susceptibility of some wild mice to X-MLVs has been mapped to the P-...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tailor CS,Nouri A,Lee CG,Kozak C,Kabat D

更新日期：1999-02-02 00:00:00

abstract：:Expansion of polyglutamine repeats in several unrelated proteins causes neurodegenerative diseases with distinct but related pathologies. To provide a model system for investigating common pathogenic features, we have examined the behavior of polyglutamine expansions expressed in Caenorhabditis elegans. The expression...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Satyal SH,Schmidt E,Kitagawa K,Sondheimer N,Lindquist S,Kramer JM,Morimoto RI

更新日期：2000-05-23 00:00:00