Abstract：
:The development of human induced pluripotent stem cell (iPSC) technology has revolutionized the regenerative medicine field. This technology provides a powerful tool for disease modeling and drug screening approaches. To circumvent the risk of random integration into the host genome caused by retroviruses, non-integrating reprogramming methods have been developed. However, these techniques are relatively inefficient or expensive. The mini-intronic plasmid (MIP) is an alternative, robust transgene expression vector for reprogramming. Here we developed a single plasmid reprogramming system which carries codon-optimized (Co) sequences of the canonical reprogramming factors (Oct4, Klf4, Sox2, and c-Myc) and short hairpin RNA against p53 ("4-in-1 CoMiP"). We have derived human and mouse iPSC lines from fibroblasts by performing a single transfection. Either independently or together with an additional vector encoding for LIN28, NANOG, and GFP, we were also able to reprogram blood-derived peripheral blood mononuclear cells (PBMCs) into iPSCs. Taken together, the CoMiP system offers a new highly efficient, integration-free, easy to use, and inexpensive methodology for reprogramming. Furthermore, the CoMIP construct is color-labeled, free of any antibiotic selection cassettes, and independent of the requirement for expression of the Epstein-Barr Virus nuclear antigen (EBNA), making it particularly beneficial for future applications in regenerative medicine.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Diecke S,Lu J,Lee J,Termglinchan V,Kooreman NG,Burridge PW,Ebert AD,Churko JM,Sharma A,Kay MA,Wu JCdoi
10.1038/srep08081subject
Has Abstractpub_date
2015-01-28 00:00:00pages
8081issn
2045-2322pii
srep08081journal_volume
5pub_type
杂志文章abstract：:To assess the correlation between penile hypersensitivity and premature ejaculation (PE), a total of 420 consecutive subjects attending our andrologic clinic for suspected PE were enrolled. The entire cohort was asked to complete the self-report intravaginal ejaculation latency time (IELT) by stopwatch. According to t...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-09155-8
更新日期：2017-09-05 00:00:00
abstract：:Amyloid precursor protein (APP) intracellular domain (AICD) is a product of APP processing with transcriptional modulation activity, whose overexpression causes various Alzheimer's disease (AD)-related dysfunctions. Here we report that 1-(3',4'-dichloro-2-fluoro[1,1'-biphenyl]-4-yl)-cyclopropanecarboxylic acid) (CHF50...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep04618
更新日期：2014-04-09 00:00:00
abstract：:Environmental contamination of arsenic (As) and its accumulation in rice (Oryza sativa L.) is of serious human health concern. In planta speciation of As is an important tool to understand As metabolism in plants. In the present study, we investigated root to shoot As translocation and speciation in rice exposed to in...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep40522
更新日期：2017-01-17 00:00:00
abstract：:Deregulated IGF-1R-AKT signaling influences multiple nodes of cancer cell physiology and assists in migration, metastasis and acquirement of radio/chemoresistance. Enrichment of cancer stem cells (CSC) positively correlates with radio/chemoresistance development in various malignancies. It is unclear though, how IGF-1...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep36612
更新日期：2016-11-07 00:00:00
abstract：:The nucleotides guanosine tetraphosphate and pentaphosphate (together known as (p)ppGpp or magic spot) are produced in plant plastids from GDP/GTP and ATP by RelA-SpoT homologue (RSH) enzymes. In the model plant Arabidopsis (p)ppGpp regulates chloroplast transcription and translation to affect growth, and is also impl...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-54207-w
更新日期：2019-11-27 00:00:00
abstract：:Pyrazinamide (PZA) is an important first-line drug in all existing and new tuberculosis (TB) treatment regimens. PZA-resistance in M. tuberculosis is increasing, especially among M/XDR cases. Noted issues with PZA Drug Susceptibility Testing (DST) have driven the search for alternative tests. This study provides a com...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-03452-y
更新日期：2017-06-19 00:00:00
abstract：:Since the 1990s the Mekong River delta has suffered a large decline in sediment supply causing coastal erosion, following catchment disturbance through hydropower dam construction and sand extraction. However, our new geological reconstruction of 2500-years of delta shoreline changes show that serious coastal erosion ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-64630-z
更新日期：2020-05-15 00:00:00
abstract：:In bacteria, selective promoter recognition by RNA polymerase is achieved by its association with σ factors, accessory subunits able to direct RNA polymerase "core enzyme" (E) to different promoter sequences. Using Chromatin Immunoprecipitation-sequencing (ChIP-seq), we searched for promoters bound by the σ(S)-associa...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep10469
更新日期：2015-05-28 00:00:00
abstract：:The discovery of CRISPR/Cas gene editing has allowed for major advances in many biomedical disciplines and basic research. One arrangement of this biotechnology, a nuclease-based gene drive, can rapidly deliver a genetic element through a given population and studies in fungi and metazoans have demonstrated the succes...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-34909-3
更新日期：2018-11-22 00:00:00
abstract：:Gastroenterologists are still unable to differentiate between some of the most ordinary disorders of the gut and consequently patients are misdiagnosed. We have developed a swallowable gas sensor capsule for addressing this. The gases of the gut are the by-product of the fermentation processes during digestion, affect...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep33387
更新日期：2016-09-16 00:00:00
abstract：:In the present study, we obtained population genetic data and forensic parameters of 30 InDel loci in Chinese Xibe ethnic group from northwestern China and studied the genetic relationships between the studied Xibe group and other reference groups. The observed heterozygosities ranged from 0.1704 at HLD118 locus to 0....
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep08260
更新日期：2015-02-05 00:00:00
abstract：:Recent studies reveal that tonal language speakers with autism have enhanced neural sensitivity to pitch changes in nonspeech stimuli but not to lexical tone contrasts in their native language. The present ERP study investigated whether the distinct pitch processing pattern for speech and nonspeech stimuli in autism w...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep43254
更新日期：2017-02-22 00:00:00
abstract：:Viral nervous necrosis (VNN) certainly represents the biggest challenge for the sustainability and the development of aquaculture. A large number of economically relevant fish species have proven to be susceptible to the disease. Conversely, gilthead sea bream has generally been considered resistant to VNN, although i...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep46755
更新日期：2017-05-02 00:00:00
abstract：:Previously, we reported that the hyperthermophilic archaeon Thermococcus onnurineus NA1 could grow on formate and produce H2. Formate conversion to hydrogen was mediated by a formate-hydrogen lyase complex and was indeed a part of chemiosmotic coupling to ATP generation. In this study, we employed an adaptation approa...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-05424-8
更新日期：2017-07-21 00:00:00
abstract：:Surface and soil core samples from northeast China were analyzed for Pu isotopes. The measured (240)Pu/(239)Pu atomic ratios and (239 + 240)Pu/(137)Cs activity ratios revealed that the global fallout is the dominant source of Pu and (137)Cs at these sites. Migration behavior of Pu varying with land type and human acti...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep03506
更新日期：2013-12-16 00:00:00
abstract：:Nitrous-oxide emission and nitrate addition from agriculture to earth's environment are two main agriculture related anthropogenic causes of environmental degradation that needs greater attention. For addressing the aforesaid issue, new techniques/practices need to be developed and implemented. The present investigati...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-021-81739-x
更新日期：2021-01-28 00:00:00
abstract：:To master linguistic communication, humans must acquire large vocabularies quickly and effortlessly. Efficient word learning might be facilitated by the ability to rapidly acquire novel word forms even outside the focus of attention, occurring within minutes of repetitive exposure and suggesting fast and automatic lex...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-34029-y
更新日期：2018-10-25 00:00:00
abstract：:Association of a high-serum ferritin with poor outcome showed that iron might play a detrimental role in the brain after intracerebral hemorrhage (ICH). Here, we investigated changes in serum iron, ferritin, transferrin (Tf) and ceruloplasmin (CP) in patients with ICH (n = 100) at day 1 (admission), 3, 7, 14 and 21 an...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep21970
更新日期：2016-02-22 00:00:00
abstract：:While concerns about human-induced effects on the Earth's climate have mainly concentrated on carbon dioxide (CO2) and methane (CH4), reducing anthropogenic nitrous oxide (N2O) flux, mainly of agricultural origin, also represents an opportunity for substantial mitigation. To develop a solution that induces neither the...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-56694-3
更新日期：2019-12-27 00:00:00
abstract：:Fire ants are widely studied, invasive and venomous arthropod pests. There is significant biomedical interest in immunotherapy against fire ant stings. However, mainly due to practical reasons, the physiological effects of envenomation has remained poorly characterized. The present study takes advantage of a recently-...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-32327-z
更新日期：2018-09-26 00:00:00
abstract：:Optical imaging and photolithography promise broad applications in nano-electronics, metrologies, and single-molecule biology. Light diffraction however sets a fundamental limit on optical resolution, and it poses a critical challenge to the down-scaling of nano-scale manufacturing. Surface plasmons have been used to ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep00175
更新日期：2011-01-01 00:00:00
abstract：:Grading of essential hypertension according to blood pressure (BP) level may not adequately reflect clinical heterogeneity of hypertensive patients. This study was carried out to explore clinical phenotypes in essential hypertensive patients using cluster analysis. This study recruited 513 hypertensive patients and ev...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep43965
更新日期：2017-03-07 00:00:00
abstract：:Formamidinium lead iodide (CH(NH2)2PbI3, FAPI) thin films have been deposited on glass substrates at 150 °C using ambient pressure aerosol assisted chemical vapour deposition (AACVD). The films have been analysed by a range of techniques including powder X-ray diffraction (pXRD), scanning electron microscopy (SEM), en...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-79291-1
更新日期：2020-12-17 00:00:00
abstract：:The combination of a solid-phase microextraction process with graphite furnace atomic absorption spectrometry provides a very sensitive determination method for determining chromium in waters. Freshly prepared ferrite particles are used to retain the chromium species, and then separated by a magnet without the need fo...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-62212-7
更新日期：2020-03-24 00:00:00
abstract：:Bacillus Calmette-Guérin (BCG) is the only licensed vaccine for tuberculosis (TB) and induces highly variable protection against pulmonary disease in different countries. We hypothesised that DNA methylation is one of the molecular mechanisms driving variability in BCG-induced immune responses. DNA methylation in peri...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-31537-9
更新日期：2018-08-30 00:00:00
abstract：:Acid-sensing ion channels (ASICs) belong to the degenerin/epithelial sodium channel protein family that form mechanosensitive ion channels. Evidence as to whether or not ASICs activity is directly modulated by mechanical force is lacking. Human ASICs (hASIC1V3, hASIC2a and hASIC3a) were heterologously expressed as hom...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-43097-7
更新日期：2019-05-01 00:00:00
abstract：:Anopheles gambiae and Aedes aegypti are perhaps the best studied mosquito species and important carriers of human malaria and arbovirus, respectively. Mosquitoes have daily rhythms in behaviors and show a wide range of activity patterns. Although Anopheles is known to be principally nocturnal and Aedes principally diu...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-61733-5
更新日期：2020-03-19 00:00:00
abstract：:Statins are well known to have muscle toxicity problem. Herba Cistanches (HC) is a Chinese herb traditionally used for pain in the loins and knees. Our previous in vitro study suggested that it could protect against statin-induced muscle toxicity. However, its in vivo protective effect has never been investigated. The...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-10008-7
更新日期：2017-08-24 00:00:00
abstract：:17α-ethynilestradiol (EE2) and tamoxifen (Tmx) are pollutants world-wide distributed in aquatic environments. Gilthead seabream, Sparus aurata L., is highlighted as a species model of intensively culture in anthropogenic disturbed environments. The effects of these pollutants on gilthead seabream reproduction and some...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-64522-2
更新日期：2020-05-14 00:00:00
abstract：:Exposure to light at night (LAN) is associated with insomnia in humans. Light provides the main input to the master clock in the hypothalamic suprachiasmatic nucleus (SCN) that coordinates the sleep-wake cycle. We aimed to develop a rodent model for the effects of LAN on sleep. Therefore, we exposed male Wistar rats t...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep35662
更新日期：2016-10-20 00:00:00
