Abstract：
:Nonstructural protein 1 (NS1) is secreted by dengue virus in the first days of infection and acts as an excellent dengue biomarker. Here, the direct electrical detection of NS1 from dengue type 2 virus has been achieved by the measurement of variations in open circuit potential (OCP) between a reference electrode and a disposable Au electrode containing immobilized anti-NS1 antibodies acting as immunosensor. Egg yolk immunoglobulin (IgY) was utilized for the first time as the biological recognition element alternatively to conventional mammalian antibodies in the detection of dengue virus NS1 protein. NS1 protein was detected in standard samples in a 0.1 to 10 µg.mL(-1) concentration range with (3.2 ± 0.3) mV/µg.mL(-1) of sensitivity and 0.09 µg.mL(-1) of detection limit. Therefore, the proposed system can be extended to detect NS1 in real samples and provide an early diagnosis of dengue.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Figueiredo A,Vieira NC,dos Santos JF,Janegitz BC,Aoki SM,Junior PP,Lovato RL,Nogueira ML,Zucolotto V,Guimarães FEdoi
10.1038/srep07865subject
Has Abstractpub_date
2015-01-19 00:00:00pages
7865issn
2045-2322pii
srep07865journal_volume
5pub_type
杂志文章abstract：:Multidomain proteins account for 70% of the eukaryotic proteome. In genetic disease, multidomain proteins are often affected by numerous mutations, but the effects of these mutations on protein stability and their roles in genetic disease are not well understood. Here, we analyzed protein globular domains to understan...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-39905-9
更新日期：2019-03-06 00:00:00
abstract：:Co-existing infection/inflammation and birth asphyxia potentiate the risk of developing neonatal encephalopathy (NE) and adverse outcome. In a newborn piglet model we assessed the effect of E. coli lipopolysaccharide (LPS) infusion started 4 h prior to and continued for 48 h after hypoxia on brain cell death and syste...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-46488-y
更新日期：2019-07-15 00:00:00
abstract：:Spectroscopic techniques combined with chemometrics methods have proven to be effective tools for the discrimination of objects with similar properties. In this work, terahertz time-domain spectroscopy (THz-TDS) combined with discriminate analysis (DA) and principal component analysis (PCA) with derivative pretreatmen...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep11115
更新日期：2015-07-08 00:00:00
abstract：:Virus pseudotyping is a useful and safe technique for studying entry of emerging strains of influenza virus. However, few studies have compared different reassortant combinations in pseudoparticle systems, or compared entry kinetics of native viruses and their pseudotyped analogs. Here, vesicular stomatitis virus (VSV...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep35537
更新日期：2016-10-18 00:00:00
abstract：:Bimetallic Pd1-x Pt x solid-solution nanoparticles (NPs) display charging/discharging of hydrogen gas, which has relevance for fuel cell technologies; however, the constituent elements are immiscible in the bulk phase. We examined these material systems using high-energy synchrotron X-ray diffraction, X-ray absorption...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-14494-7
更新日期：2017-11-06 00:00:00
abstract：:The fusion of villous cytotrophoblasts into the multinucleated syncytiotrophoblast is critical for the essential functions of the mammalian placenta. Using RNA-Seq gene expression and quantitative protein expression, we identified genes and their cognate proteins which are coordinately up- or down-regulated in two cel...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-23379-2
更新日期：2018-03-23 00:00:00
abstract：:Cerebral aneurysms are abnormal focal dilatations of arterial vessel walls with pathological vessel structure alterations. Sudden rupture can lead to a subarachnoid hemorrhage, which is associated with a high mortality. Therefore, the origin of cerebral aneurysms as well as the progression to the point of rupture need...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-69222-5
更新日期：2020-07-23 00:00:00
abstract：:Many environmental flow (e-flow) studies and applications have predominantly used state-(i.e., at a single time point) and rate-(i.e., temporal change) based demographic characteristics of species representing lower trophic levels (e.g., fish communities) to build flow-ecology relationships, rather than using a proces...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-79532-3
更新日期：2020-12-18 00:00:00
abstract：:The inner ear, or cochlea, is a fluid-filled organ housing the mechanosensitive hair cells. Sound stimulation is relayed to the hair cells through waves that propagate on the elastic basilar membrane. Sensorineural hearing loss occurs from damage to the hair cells and cannot currently be cured. Although drugs have bee...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-79946-z
更新日期：2021-01-08 00:00:00
abstract：:More than 20 years after the first outbreaks, the phylogenetic picture of PRRSV is still incomplete and full of gaps, especially in regards of PRRSV 1. Due to the exceptional diversity observed at the eastern borders of Europe and the low number of available sequences from Central Eastern European countries, the autho...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-26036-w
更新日期：2018-05-17 00:00:00
abstract：:A high specificity aptamer-ligand biorecognition and binding system to monitor of dexamethasone (DXN) was developed. The detection principle was based on a label-free electrochemical aptasensor. The selection of the aptamer was successfully performed by the systematic evolution of ligands through exponential enrichmen...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-42671-3
更新日期：2019-04-29 00:00:00
abstract：:Next generation sequencing is transforming clinical medicine and genome research, providing a powerful route to establishing molecular diagnoses for genetic conditions; however, challenges remain given the volume and complexity of genetic variation. A number of methods integrate patient phenotype and genotypic data to...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-13841-y
更新日期：2017-10-18 00:00:00
abstract：:Physiological regulation of blood flow in bone marrow is important to maintain oxygen and glucose supplies but also the physiological hypoxic state of the hematopoietic stem cell (HSC) niche. However, regulatory mechanisms underlying microcirculation in the bone marrow (BM) niche remain unclear. Here, we identify vess...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-63533-3
更新日期：2020-04-14 00:00:00
abstract：:Envelope (E) and temporal fine structure (TFS) are important features of acoustic signals and their corresponding perceptual function has been investigated with various listening tasks. To further understand the underlying neural processing of TFS, experiments in humans and animals were conducted to demonstrate the ef...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-12975-3
更新日期：2017-10-17 00:00:00
abstract：:PDCD4, the protein encoded by the tumor suppressor gene PDCD4 (programmed cell death 4) has been implicated in the control of cellular transcription and translation by modulating the activity of specific transcription factors and suppressing the translation of mRNAs with structured 5'-UTRs. Most studies of human PDCD4...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-59678-w
更新日期：2020-02-17 00:00:00
abstract：:Most of the human genome is non-coding. However, some of the non-coding part is transcriptionally active. In humans, the tandemly repeated (TR) pericentromeric non-coding DNA-human satellites 2 and 3 (HS2, HS3)-are transcribed in somatic cells. These transcripts are also found in pre- and post-implantation embryos. Th...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-76628-8
更新日期：2020-11-12 00:00:00
abstract：:We study a minimal model of the stress-driven p53 regulatory network that includes competition between active and mutant forms of the tumor-suppressor gene p53. Depending on the nature and level of the external stress signal, four distinct dynamical states of p53 are observed. These states can be distinguished by diff...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-021-82054-1
更新日期：2021-01-27 00:00:00
abstract：:The use of O-(2-[18F]fluoroethyl)-L-tyrosine ([18F]FET) as a positron emission tomography (PET) tracer for brain tumor imaging might have some limitations because of the relatively low affinity for the L-type amino acid transporter 1 (LAT1). To assess the stereospecificity and evaluate the influence of aromatic ring m...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-40013-x
更新日期：2019-02-27 00:00:00
abstract：:Excessive or misdirected artificial light at night (ALAN) produces light pollution that influences several aspects of the biology and ecology of birds, including disruption of circadian rhythms and disorientation during flight. Many migrating birds traverse large expanses of land twice every year at night when ALAN il...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-21577-6
更新日期：2018-02-19 00:00:00
abstract：:Exhibiting a red-shifted absorption/scattering feature compared to conventional plasmonic metals, titanium nitride nanoparticles (TiN NPs) look as very promising candidates for biomedical applications, but these applications are still underexplored despite the presence of extensive data for conventional plasmonic coun...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-37519-1
更新日期：2019-02-04 00:00:00
abstract：:Sleep, in addition to its brain restorative processes, plays an important role in memory transfer from its temporary store in the hippocampus to the more permanent storage in the neocortex. Alzheimer's disease (AD) affects memory and sleep. The aim of this study was to explore disturbances in global and local synchron...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-41851-5
更新日期：2019-03-29 00:00:00
abstract：:Grain boundaries (GBs) in graphene prepared by chemical vapor deposition (CVD) greatly degrade the electrical and mechanical properties of graphene and thus hinder the applications of graphene in electronic devices. The seamless stitching of graphene flakes can avoid GBs, wherein the identical orientation of graphene ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep06541
更新日期：2014-10-07 00:00:00
abstract：:Calcium ion is an intracellular messenger that plays a central role in signal transduction pathways. Calcineurin B-like proteins (CBLs) and CBL-interacting protein kinases (CIPKs) signal network have shown different functions in the Ca(2+) signaling process. In this work, we identified the entire soybean (Glycine max)...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep28225
更新日期：2016-06-17 00:00:00
abstract：:Soluble oligomeric assemblies of amyloidal proteins appear to act as major pathological agents in several degenerative disorders. Isolation and characterization of these oligomers is a pivotal step towards determination of their pathological relevance. Here we describe the isolation of Type 2 diabetes-associated islet...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep04267
更新日期：2014-03-04 00:00:00
abstract：:To comprehensively assess whether p75ECD in urine could be a candidate biomarker for ALS evaluation. Urine samples were collected from 101 ALS patients, 108 patients with other neurological disease (OND) and 97 healthy controls. 61 ALS patients were followed up with clinical data including ALSFRS-r every 6 to 12 month...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-05430-w
更新日期：2017-07-11 00:00:00
abstract：:Sampling the concentration of insulin in human skin using microdialysis is challenging because of low intracutaneous concentrations and low recovery, presumably due to adsorption of insulin to the microdialysis system. In this study, we aimed to (1) measure how the concentration of insulin varies in three different ti...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-78728-x
更新日期：2020-12-15 00:00:00
abstract：:We demonstrate synthesis of Ni/CuOx/Ni nanowires (NWs) by electrochemical deposition on anodized aluminum oxide (AAO) membranes. AAO with pore diameter of ~70 nm and pore length of ~50 μm was used as the template for synthesis of NWs. After deposition of Au as the seed layer, NWs with a structure of Ni/CuOx/Ni were gr...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep23069
更新日期：2016-03-15 00:00:00
abstract：:Monitoring marine top predators is fundamental for assessing the health and functioning of open ocean ecosystems. Although recently tracking observations have substantially increased, factors determining the horizontal exploration of the ocean by marine predators are still largely unknown, especially at the scale of b...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep18063
更新日期：2015-12-15 00:00:00
abstract：:Exercise has been considered as treatment and a preventive modality to alleviate depressive symptoms, but sex differences regarding specific types of exercise in association with depression have not been clearly elucidated. Here, we investigated sex differences in the association between exercise type and depression i...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-75389-8
更新日期：2020-10-26 00:00:00
abstract：:Magnesium has been shown to effectively prevent vascular calcification associated with chronic kidney disease. Magnesium has been hypothesized to prevent the upregulation of osteoblastic genes that potentially drives calcification. However, extracellular effects of magnesium on hydroxyapatite formation are largely neg...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-20241-3
更新日期：2018-02-01 00:00:00
