Abstract：
:Excitatory amino acids (EAAs) are important neurotransmitters in the vertebrate central nervous system. Electrophysiological and ligand-binding studies indicate that at least three different receptor subtypes for EAAs exist--N-methyl-D-aspartate, kainate and quisqualate receptor subtypes--on the basis of the preferred agonist of the receptors. We recently purified a kainate-binding protein (KBP) from frog (Rana pipiens berlandieri) brain by domoic acid (a high-affinity kainate analogue) affinity chromatography, and showed that the kainate-binding activity was associated with a protein of relative molecular mass 48,000 (Mr 48 K). The pharmacological properties and the anatomical distribution of KBP were consistent with those of a kainate receptor-ionophore complex. We have now isolated a complementary DNA encoding KBP of Mr 48 K. The deduced amino-acid sequence of the KBP has similar hydrophobic profiles to those found in other ligand-gated ion channel subunits, and shows some amino-acid sequence similarities to the corresponding regions of brain nicotinic acetylcholine receptor subunits. Localization of the KBP messenger RNAs by in situ hybridization histochemistry is compatible with the results of immunohistochemistry and receptor autoradiography studies. COS-7 cells transfected with the cDNA encoding the KBP show high-affinity kainate-binding activity with pharmacological properties similar to those of the biochemically purified KBP. These results provide the first molecular characterization of an EAA-binding site and raise the possibility that the KBP cDNA encodes a ligand-binding subunit of a kainate receptor-ionophore complex.
journal_name
Naturejournal_title
Natureauthors
Wada K,Dechesne CJ,Shimasaki S,King RG,Kusano K,Buonanno A,Hampson DR,Banner C,Wenthold RJ,Nakatani Ydoi
10.1038/342684a0subject
Has Abstractpub_date
1989-12-07 00:00:00pages
684-9issue
6250eissn
0028-0836issn
1476-4687journal_volume
342pub_type
杂志文章相关文献
NATURE文献大全abstract：:Recognition of objects or environmental landmarks is problematic because appearance can vary widely depending on illumination, viewing distance, angle of view and so on. Storing a separate image or 'template' for every possible view requires vast numbers to be stored and scanned, has a high probability of recognition ...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/35001064
更新日期：2000-02-10 00:00:00
abstract：: ...
journal_title：Nature
pub_type： 新闻
doi：10.1038/d41586-018-07631-3
更新日期：2018-12-01 00:00:00
abstract：:Representations of sensory stimuli in the cerebral cortex can undergo progressive remodelling according to the behavioural importance of the stimuli. The cortex receives widespread projections from dopamine neurons in the ventral tegmental area (VTA), which are activated by new stimuli or unpredicted rewards, and are ...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/35083586
更新日期：2001-07-05 00:00:00
abstract：:Gene targeting in embryonic stem cells has become the principal technology for manipulation of the mouse genome, offering unrivalled accuracy in allele design and access to conditional mutagenesis. To bring these advantages to the wider research community, large-scale mouse knockout programmes are producing a permanen...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature10163
更新日期：2011-06-15 00:00:00
abstract：:Numerous post-translational modifications of histones have been described in organisms ranging from yeast to humans. Growing evidence for dynamic regulation of these modifications, position- and modification-specific protein interactions, and biochemical crosstalk between modifications has strengthened the 'histone co...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature06906
更新日期：2008-06-05 00:00:00
abstract：:'Identifier' or ID sequences are present in 62% of the RNA polymerase II and III transcripts made in vitro from brain nuclei but in fewer than 4% of the transcripts made from the nuclei of other tissues. An homologous 160-nucleotide cytoplasmic poly(A)+ RNA species, BC1, and a smaller species, BC2, are located in vivo...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/308237a0
更新日期：1984-03-15 00:00:00
abstract：:The normal cellular form of prion protein (PrPC) is a precursor to the pathogenic protease-resistant forms (PrPSc) believed to cause scrapie, bovine spongiform encephalopathy (BSE) and Creutzfeldt-Jakob disease. Its amino terminus contains the octapeptide PHGGGWGQ, which is repeated four times and is among the best-pr...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/37783
更新日期：1997-12-18 00:00:00
abstract：:The ATP-dependent chromatin assembly and remodelling factor (ACF) functions to generate regularly spaced nucleosomes, which are required for heritable gene silencing. The mechanism by which ACF mobilizes nucleosomes remains poorly understood. Here we report a single-molecule FRET study that monitors the remodelling of...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature08627
更新日期：2009-12-24 00:00:00
abstract：:How the brain selects appropriate sensory inputs and suppresses distractors is unknown. Given the well-established role of the prefrontal cortex (PFC) in executive function, its interactions with sensory cortical areas during attention have been hypothesized to control sensory selection. To test this idea and, more ge...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature15398
更新日期：2015-10-29 00:00:00
abstract：:The discovery that the primary transcripts of many genes are processed to produce more than one kind of messenger RNA focuses attention on the control of RNA processing as a developmental regulatory mechanism. In the fruit-fly Drosophila melanogaster, differential splicing of a hierarchy of regulatory genes determines...
journal_title：Nature
pub_type： 杂志文章,评审
doi：10.1038/340521a0
更新日期：1989-08-17 00:00:00
abstract：:Polycomb and trithorax group (PcG and trxG) proteins maintain silent and active transcriptional states, respectively, throughout development. In Drosophila, PcG and trxG proteins associate with DNA regions named Polycomb and trithorax response elements (PRE and TRE), but the mechanisms of recruitment are unknown. We p...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature03386
更新日期：2005-03-24 00:00:00
abstract：:The innate immune system is critical for the early detection of invading pathogens and for initiating cellular host defence countermeasures, which include the production of type I interferon (IFN). However, little is known about how the innate immune system is galvanized to respond to DNA-based microbes. Here we show ...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature08476
更新日期：2009-10-08 00:00:00
abstract：:Caste differentiation and division of labour are the hallmarks of insect societies and at the root of their ecological success. Kin selection predicts that caste determination should result from environmentally induced differences in gene expression, a prediction largely supported by empirical data. However, two excep...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature01744
更新日期：2003-07-17 00:00:00
abstract：:The wavelength of light represents a fundamental technological barrier to the production of increasingly smaller features on integrated circuits. New technologies that allow the replication of patterns on scales less than 100 nm need to be developed if increases in computing power are to continue at the present rate. ...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/35002540
更新日期：2000-02-24 00:00:00
abstract：:Recent studies have suggested that bone marrow cells possess a broad differentiation potential, being able to form new liver cells, cardiomyocytes and neurons. Several groups have attributed this apparent plasticity to 'transdifferentiation'. Others, however, have suggested that cell fusion could explain these results...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature02069
更新日期：2003-10-30 00:00:00
abstract：:RecBCD is a multi-functional enzyme complex that processes DNA ends resulting from a double-strand break. RecBCD is a bipolar helicase that splits the duplex into its component strands and digests them until encountering a recombinational hotspot (Chi site). The nuclease activity is then attenuated and RecBCD loads Re...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature02988
更新日期：2004-11-11 00:00:00
abstract：:Nuclear-architecture defects have been shown to correlate with the manifestation of a number of human diseases as well as ageing. It is therefore plausible that diseases whose manifestations correlate with ageing might be connected to the appearance of nuclear aberrations over time. We decided to evaluate nuclear orga...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature11557
更新日期：2012-11-22 00:00:00
abstract：:Rheumatoid arthritis is a chronic autoinflammatory disease that affects 1-2% of the world's population and is characterized by widespread joint inflammation. Interleukin-1 is an important mediator of cartilage destruction in rheumatic diseases, but our understanding of the upstream mechanisms leading to production of ...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature13322
更新日期：2014-08-07 00:00:00
abstract：:Excitatory amino acids mediate fast synaptic transmission in the central nervous system through the activation of at least three distinct ionotropic receptors: N-methyl-D-aspartate (NMDA), the alpha-amino-3-hydroxy-5-methyl-isoxasole-4-propionate (AMPA)/quisqualate (QUIS) and the kainate subtypes (for reviews, see ref...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/347765a0
更新日期：1990-10-25 00:00:00
abstract：:Solitons are large-amplitude, spatially confined wave packets in nonlinear media. They occur in a wide range of physical systems, such as water surfaces, optical fibres, plasmas, Bose-Einstein condensates and magnetically ordered media. A distinguishing feature of soliton behaviour that is common to all systems, is th...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature02042
更新日期：2003-11-13 00:00:00
abstract：:The silicate Earth is strongly depleted in moderately volatile elements (such as lead, zinc, indium and alkali elements) relative to CI chondrites, the meteorites that compositionally most closely resemble the Sun. This depletion may be explained qualitatively by accretion of 10 to 20 per cent of a volatile-rich body ...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature23645
更新日期：2017-09-27 00:00:00
abstract：:Although the sex-determining gene Sry has been identified in mammals, no comparable genes have been found in non-mammalian vertebrates. Here, we used recombinant breakpoint analysis to restrict the sex-determining region in medaka fish (Oryzias latipes) to a 530-kilobase (kb) stretch of the Y chromosome. Deletion anal...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature751
更新日期：2002-05-30 00:00:00
abstract：:Valuable information about proto-oncogenes and their physiological function has been obtained by studying their expression in normal cells. However, expression of the c-mos gene, the cellular homologue of the transforming gene of Moloney murine sarcoma virus, has not been detected in normal mouse cells or tissues. The...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/315516a0
更新日期：1985-06-06 00:00:00
abstract：:Online hate and extremist narratives have been linked to abhorrent real-world events, including a current surge in hate crimes1-6 and an alarming increase in youth suicides that result from social media vitriol7; inciting mass shootings such as the 2019 attack in Christchurch, stabbings and bombings8-11; recruitment o...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/s41586-019-1494-7
更新日期：2019-09-01 00:00:00
abstract：:The type III secretion (T3S) injectisome is a specialized protein nanomachine that is critical for the pathogenicity of many Gram-negative bacteria, including purveyors of plague, typhoid fever, whooping cough, sexually transmitted infections and major nosocomial infections. This syringe-shaped 3.5-MDa macromolecular ...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature20576
更新日期：2016-12-22 00:00:00
abstract：:Inhibitory neurotransmission mediated by GABA(A) receptors can be modulated by the endogenous neurosteroids, allopregnanolone and tetrahydro-deoxycorticosterone. Neurosteroids are synthesized de novo in the brain during stress, pregnancyand after ethanol consumption, and disrupted steroid regulation of GABAergic trans...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature05324
更新日期：2006-11-23 00:00:00
abstract：:B LYMPHOCYTES are key participants in the immune response because of their specificity, their ability to take up and present antigens to T cells, and their capacity to differentiate into antibody-secreting cells. To limit reactivity to self antigens, autospecific B cells can be functionally inactivated or deleted. Dev...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/354308a0
更新日期：1991-11-28 00:00:00
abstract：:Serine proteases are present in virtually all organisms and function both inside and outside the cell; they exist as two families, the 'trypsin-like' and the 'subtilisin-like', that have independently evolved a similar catalytic device characterized by the Ser, His, Asp triad, an oxyanion binding site, and possibly ot...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/332564a0
更新日期：1988-04-07 00:00:00
abstract：:In the response of T cells to foreign antigens, the ligand for the T cell alpha/beta receptor is presented on a cell surface as a fragment of antigen complexed to one of the membrane molecules encoded in the major histocompatibility complex (MHC). The receptor apparently interacts via its variable elements (V beta, D ...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/332840a0
更新日期：1988-04-28 00:00:00
abstract：:A basic requirement for quantum information processing systems is the ability to completely control the state of a single qubit. For qubits based on electron spin, a universal single-qubit gate is realized by a rotation of the spin by any angle about an arbitrary axis. Driven, coherent Rabi oscillations between two sp...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature07530
更新日期：2008-11-13 00:00:00
