Abstract:
:Previously, we evaluated the effects of lactational exposure to a representative mixture of the six indicator non-dioxin-like polychlorinated biphenyls (∑6 NDL-PCBs) at low levels on the neurobiological changes and developmental/behavioral performances in mice. In this study, we analyzed the global gene expression profile in cerebellar neurons isolated from male mice presenting the most significant induction of anxiety-like behavior in our previous study (10 ng/kg ∑6 NDL-PCBs). Our results revealed changes in the expression of 16658 genes in the neurons of the exposed mice. Among these, 693 upregulated [fold change (FC)>2; p<0.05] and 665 downregulated (FC<2; p<0.05) genes were statistically linked to gene ontology terms (GO). Overexpressed genes belonged to GO terms involved with the cell cycle, DNA replication, cell cycle checkpoint, response to DNA damage stimulus, regulation of RNA biosynthetic processes, and microtubule cytoskeleton organization. Downregulated genes belonged to terms involved with the transmission of nerve impulses, projection neurons, synapse hands, cell junctions, and regulation of RNA biosynthetic processes. Using qPCR, we quantified gene expression related to DNA damage and validated the transcriptomic study, as a significant overexpression of Atm-Atr Bard1, Brca2, Fancd2, Figf, Mycn, p53 and Rad51 was observed between groups (p<0.001). Finally, using immunoblots we determined the expression level of six selected proteins. We found that changes in the protein expression of Atm Brca1, p53, Kcnma1, Npy4r and Scn1a was significant between exposed and control groups (p<0.05), indicating that the expression pattern of these proteins agreed with the expression pattern of their genes by qPCR, further validating our transcriptomic findings. In conclusion, our study showed that early life exposure of male mice to a low level of ∑6 NDL-PCBs induced p53-dependent responses to cellular stress and a decrease in the expression of proteins involved in the generation, conduction, and transmission of electrical signals in neurons.
journal_name
Toxicologyjournal_title
Toxicologyauthors
Elnar AA,Desor F,Marin F,Soulimani R,Nemos Cdoi
10.1016/j.tox.2014.12.011subject
Has Abstractpub_date
2015-02-03 00:00:00pages
57-65eissn
0300-483Xissn
1879-3185pii
S0300-483X(14)00241-8journal_volume
328pub_type
杂志文章相关文献
TOXICOLOGY文献大全abstract::Recently we observed a suppressed delayed-type hypersensitivity (DTH) response to bovine serum albumin (BSA) in the 4-5-month-old offspring of F344 rat dams receiving as little as 1.0 microg 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)/kg on gestational day (GD) 14. This study was designed to characterize better this su...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/s0300-483x(99)00024-4
更新日期:1999-05-03 00:00:00
abstract::The subacute inhalation toxicity of butenolide was examined in hamsters by repeated exposure of 4 groups of 10 males and 10 females to butenolide vapour at concentrations of 0, 5.4, 25 and 130 ppm respectively (6 h/day, 5 days/week) for a period of 13 weeks. The effects found at 130 ppm included eye irritation, saliva...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/0300-483x(79)90020-9
更新日期:1979-12-01 00:00:00
abstract::The nerve agent tabun inhibits the essential enzyme acetylcholinesterase (AChE) by a rapid phosphoramidation of the catalytic serine residue. Oximes, such as K027 and HLö-7, can reactivate tabun-inhibited human AChE (tabun-hAChE) whereas the activity of their close structural analogue HI-6 is notably low. To investiga...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2009.09.002
更新日期:2009-11-30 00:00:00
abstract::Age-related differences in susceptibility to cephaloridine nephrotoxicity were evaluated in male Fischer-344 rats. Rats, 2.5, 4, 10-12 and 27-29 months old, were administered a single intraperitoneal dose of cephaloridine and renal function evaluated 24 h later. Susceptibility to cephaloridine-induced nephrotoxicity w...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/0300-483x(86)90171-x
更新日期:1986-01-01 00:00:00
abstract::Palmitoylpentachlorophenol (PPCP), which is a lipid conjugate of a xenobiotic compound, has been found in human fat. To study the toxicity associated with PPCP, rats were given 100 mg/kg PPCP and sacrificed at 4, 8 and 12 days. The target organ identified was the exocrine pancreas; no other major organs examined showe...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/0300-483x(87)90137-5
更新日期:1987-10-12 00:00:00
abstract::Recent attention to methanol (MeOH) as a potential alternative fuel prompted an evaluation of the chemical's effects during early pregnancy. Rats were dosed by gavage during Days 1-8 of pregnancy at 0, 1.6, 2.4, or 3.2 g MeOH/kg/day. Groups of animals were killed on Days 9, 11, or 20 of pregnancy, and maternal, embryo...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/0300-483x(93)90212-b
更新日期:1993-05-24 00:00:00
abstract::Different affinities of blood for CO were observed in in vivo and in vitro experiments. Hamsters showed the greatest relative affinity (1.0), the corresponding value for rats was 0.8, for pigs 0.74 and for rabbits 0.58. The upward shift of the saturation curves was dependent on the rate of respiratory exchange per uni...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/0300-483x(75)90031-1
更新日期:1975-01-01 00:00:00
abstract::Gulf War Illness (GWI) affects 25% of veterans from the 1990-1991 Gulf War (GW) and is accompanied by damage to the brain regions involved in memory processing. After twenty-five years, the chronic pathobiology of GWI is still unexplained. To address this problem, we examined the long-term consequences of GW exposures...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2016.10.012
更新日期:2016-11-30 00:00:00
abstract::Thiols are known to influence the metabolism of glutathione. In a previous study (Toxicology 156 (2001) 93) dithiothreitol (DTT) did not show any effect on intra- or extracellular glutathione concentrations in HeLa cell cultures but increased the effects of mercury ions on glutathione concentrations, whereas monothiol...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/s0300-483x(02)00060-4
更新日期:2002-06-14 00:00:00
abstract::Bisphenol A (BPA), is an artificial estrogen initially produced for medical purposes but is today widely used in polycarbonate plastics and epoxy resins. Exposure-related reproductive disorders have been found, but recently it has also been suggested that BPA may be involved in obesity, diabetes, myocardial hypertroph...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2016.02.007
更新日期:2016-03-10 00:00:00
abstract::Studies on the pulmonary retention of benzo[a]pyrene after inhalation have shown that clearance is biphasic, with one component clearing with a half-time greater than 1 day and another with a half-time less than 1 day. In the work reported here we demonstrated that the amount of benzo[a]pyrene instilled in the lungs c...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/0300-483x(85)90066-6
更新日期:1985-06-28 00:00:00
abstract::Malathion is one of the most widely used organophosphorus insecticides in agriculture. However, malathion may be involved in the etiology of human brain dysfunction. Induction of ROS has been proposed as a mechanism of malathion-induced poisoning cases, but there are few data regarding the effects of malathion on oxid...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2019.02.004
更新日期:2019-04-01 00:00:00
abstract::Inhibition of fungal lanosterol-14 alpha-demethylase (CYP51) is the working principle of the antifungal activity of azoles used in agriculture and medicine. Inhibition of human CYP51 may result in endocrine disruption since follicular fluid-meiosis activating steroid (FF-MAS), the direct product of lanosterol demethyl...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2006.08.007
更新日期:2006-11-10 00:00:00
abstract::Mutagenicity analysis of urine from rats treated by oral gavage with MX at a dose of 64 mg/kg for 14 days revealed that only 0.3% of the administered compound was excreted in a genotoxically active form. At lower doses, mutagenicity was not detectable. No evidence of micronucleus induction in peripheral blood erythroc...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/0300-483x(96)03336-7
更新日期:1996-06-17 00:00:00
abstract::Hexachlorobenzene (HCB) is an organochlorine pesticide that acts as an endocrine disruptor in humans and rodents. The development of breast cancer strongly depends on endocrine conditions modulated by environmental factors. We have demonstrated that HCB is a tumor co-carcinogen in rats and an inducer of proliferation ...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2011.12.012
更新日期:2012-03-11 00:00:00
abstract::Adult female F-344 rats were trained (avoidance rate > 70%) over four days with a coupled tone- (n = 10 rats/dose) or 2 ppm acetaldehyde-cued (n = 6 rats/dose) foot shock paradigm. Rats were gavaged with chloroform dissolved in corn oil for 5 days/week for 3 week at 0 or 400 (tone-cued) or 0, 34, 100, or 400 (odor-cue...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/s0300-483x(97)00076-0
更新日期:1997-09-26 00:00:00
abstract::Thimerosal (TH), an ethylmercury complex of thiosalicylic acid has been used as preservative in vaccines. In vitro neurotoxicity of TH at high nM concentrations has been reported. Although a number of toxicological experiments demonstrated high affinity of mercury to thiol groups of the extracellular amino acids and p...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2010.07.023
更新日期:2010-10-29 00:00:00
abstract::Though, oxidative stress has been implicated in silica nanoparticles induced toxicity both in vitro and in vivo, but no similarities exist regarding dose-response relationship. This discrepancy may, partly, be due to associated impurities of trace metals that may present in varying amounts. Here, cytotoxicity and oxid...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2010.07.010
更新日期:2010-10-09 00:00:00
abstract::Deltamethrin, an alpha-cyano type II synthetic pyrethroid insecticide is used to control a wide range of insects on a variety of crops. Deltamethrin is reported to cause many adverse effects on non-target species. Deltamethrin is reported to cause DNA damage and micronuclei induction in human lymphocytes. It is highly...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/s0300-483x(00)00416-9
更新日期:2001-05-28 00:00:00
abstract::Maize co-contamination with aflatoxin B1 (AFB1) and fumonisin B1 (FB1) is frequently found in several countries. Although the alterations on nutritional and immunologic parameters induced by these mycotoxins, when administered individually, are partially characterised, little is known about the effects induced in anim...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/s0300-483x(02)00603-0
更新日期:2003-04-15 00:00:00
abstract::The teratogenicity of antiepilepsy drug valproic acid (VPA) mostly is found in genetic and somatic levels, causing teratogenesis involving neurotubular defects (NTDs), anencephaly, lumbosacral meningomyelocele, and leg dysfunction due to spina bifida aperta. A diversity of nutraceutics have been tried to alleviate the...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2011.10.015
更新日期:2012-01-27 00:00:00
abstract::Tetrahydrocannabinol (THC), a psychoactive component of marijuana, is known to exert cytotoxicity in immune cells. In the present study, we examined the cytotoxicity of Δ⁸-THC in mouse macrophage J774-1 cells and a possible involvement of cannabinoid receptors and stress-responsive mitogen-activated protein kinases (M...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2013.10.007
更新日期:2013-12-15 00:00:00
abstract::Nitrated-polycyclic aromatic hydrocarbons (nitro-PAHs) and diesel exhaust particle extracts (DEPE) induced apoptosis in Hepa1c1c7 cells with the following potency: 1,3-dinitropyrene (1,3-DNP)>1-nitropyrene (1-NP) > DEPE > 1,8-dinitropyrene (1,8-DNP). The compounds induced cyp1a1, and activated various intracellular si...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2006.12.009
更新日期:2007-03-07 00:00:00
abstract::To elucidate factors responsible for altered proliferation of preneoplastic hepatocytes in rat hepatocarcinogenesis in vivo, EGF-stimulated DNA synthesis of normal and nodular hepatocytes in primary culture was studied. In addition, the influence of 2,3,7, 8-tetrachlorodibenzo-p-dioxin (TCDD) was investigated to clari...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/s0300-483x(99)00189-4
更新日期:2000-04-03 00:00:00
abstract::Antidepressants are generally used for treatment of various mood and anxiety disorders. Several studies have shown the anti-tumor and cytotoxic activities of some antidepressants, but the underlying mechanisms were unclear. Maprotiline is a tetracyclic antidepressant and possesses a highly selective norepinephrine reu...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2012.11.013
更新日期:2013-02-08 00:00:00
abstract:UNLABELLED:We recently observed a case of digoxin and insulin self-poisoning without cardiac repercussion. We raised the hypothesis that insulin may have a cardio-protective effect in case of digoxin toxicity. We have therefore evaluated the effect of glucose-insulin infusion on mortality and ECG abnormalities during a...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2006.04.035
更新日期:2006-07-25 00:00:00
abstract::The agricultural fungicide, N-(3,5-dichlorophenyl)succinimide (NDPS) induces acute polyuric renal failure which is attenuated by pretreatment with the glutathione depletors, diethyl maleate or buthionine sulfoximine (BSO). In the present study, the temporal aspects of BSO attenuation of NDPS nephrotoxicity were invest...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/s0300-483x(96)03581-0
更新日期:1997-02-28 00:00:00
abstract::The bioavailability of coplanar 2,3',4,4',5-pentachlorobiphenyl (PCB118) and nonplanar 2,2',5,5'-tetrachlorobiphenyl (PCB52) from soils representing a range in organic carbon (OC), clay content and pH were investigated using an in vivo rat model and an in vitro physiologically based extraction test (PBET) to assess th...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2005.08.012
更新日期:2006-01-05 00:00:00
abstract::The present study investigates the influence of different levels of Schistosoma mansoni infection (60, 120, 180, 300, 600 cercariae per mice) after 33 days on the activity of aryl hydrocarbon hydroxylase (AHH) and the formation of hepatic hydrogen peroxide (H(2)O(2)) during the metabolic activation of benzo(a)pyrene [...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/s0300-483x(01)00372-9
更新日期:2001-06-21 00:00:00
abstract::Stannous chloride (SnCl2) is a reducing chemical agent used in several man-made products. SnCl2 can generate reactive oxygen species (ROS). Therefore, the present study has been carried out to investigate the antioxidant action of l-ascorbic acid (AA) in minimizing SnCl2 toxicity on lipid peroxidation, antioxidant enz...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2007.03.017
更新日期:2007-06-25 00:00:00