Abstract:
:The myogenic regulatory genes Myf5, Mrf4, Myod, and Myogenin likely arose by gene duplications during evolution, presumably to address the more demanding requirements of the vertebrate body plan. Two cell lineages were proposed to be regulated independently by Myf5 and Myod to safeguard against tissue failure. Here we report severe muscle loss following ablation of Myf5-expressing cells. Using both lineage-specific and ubiquitous reporter alleles, we show that the remaining muscles in Myf5(Cre)-DTA embryos arise mainly from Myf5(+) escaper cells. Elimination of Myf5(Cre)-DTA cells on a Myod null background did not result in the total absence of skeletal muscles, as would be expected if a Myod(+)/Myf5-independent cell population played a major role in this scenario. Therefore, these observations are incompatible with a previously proposed functional two-lineage model. These findings will have an impact on the interpretation of phenotypes obtained using similar strategies in other tissues.
journal_name
Dev Celljournal_title
Developmental cellauthors
Comai G,Sambasivan R,Gopalakrishnan S,Tajbakhsh Sdoi
10.1016/j.devcel.2014.11.005subject
Has Abstractpub_date
2014-12-08 00:00:00pages
654-67issue
5eissn
1534-5807issn
1878-1551pii
S1534-5807(14)00722-9journal_volume
31pub_type
杂志文章abstract::RNA-binding Fox (Rbfox) proteins have well-established roles in regulating alternative splicing, but specific Rbfox isoforms lack nuclear localization signals and accumulate in the cytoplasm. The potential splicing-independent functions of these proteins remain unknown. Here we demonstrate that cytoplasmic Drosophila ...
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abstract::The role of Lefty2 in left-right patterning was investigated by analysis of mutant mice that lack asymmetric expression of lefty2. These animals exhibited various situs defects including left isomerism. The asymmetric expression of nodal was prolonged and the expression of Pitx2 was upregulated in the mutant embryos. ...
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journal_title:Developmental cell
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journal_title:Developmental cell
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journal_title:Developmental cell
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journal_title:Developmental cell
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journal_title:Developmental cell
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doi:10.1016/j.devcel.2020.01.028
更新日期:2020-04-06 00:00:00
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journal_title:Developmental cell
pub_type: 评论,杂志文章
doi:10.1016/j.devcel.2016.02.002
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journal_title:Developmental cell
pub_type:
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journal_title:Developmental cell
pub_type: 杂志文章
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journal_title:Developmental cell
pub_type: 评论,杂志文章
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