Abstract：
:Monoterpenoids are a diverse class of natural products and contribute to the important varietal aroma of certain Vitis vinifera grape cultivars. Among the typical monoterpenoids, linalool exists in almost all grape varieties. A gene coding for a nerolidol/linalool (NES/LINS) synthase was evaluated in the role of linalool biosynthesis in grape berries. Enzyme activity assay of this recombinant protein revealed that it could convert geranyl diphosphate and farnesyl diphosphate into linalool and nerolidol in vitro, respectively, and thus it was named VvRILinNer. However, localization experiment showed that this enzyme was only localized to chloroplasts, which indicates that VvRILinNer functions in the linalool production in vivo. The patterns of gene expression and linalool accumulation were analyzed in the berries of three grape cultivars ("Riesling", "Cabernet Sauvignon", "Gewurztraminer") with significantly different levels of monoterpenoids. The VvRILinNer was considered to be mainly responsible for the synthesis of linalool at the early developmental stage. This finding has provided us with new knowledge to uncover the complex monoterpene biosynthesis in grapes.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Zhu BQ,Cai J,Wang ZQ,Xu XQ,Duan CQ,Pan QHdoi
10.3390/ijms151221992subject
Has Abstractpub_date
2014-12-01 00:00:00pages
21992-2010issue
12issn
1422-0067pii
ijms151221992journal_volume
15pub_type
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