Abstract:
:The present study deals with improvement of the mucoadhesive properties of monoolein based cubic nanoparticles by incorporating chitosan. Chitosan-tailored cubic nanoparticles were prepared by thin film hydration followed by ultrasonication employing clotrimazole as model drug. The effect of Pluronic F127 fraction and concentration of chitosan on particle size and % mucin binding of the formulations was studied using 2-factor, 3-level, central composite experimental design. The concentration of chitosan was found to influence particle size and % mucin binding of cubic nanoparticles while Pluronic F127 fraction influenced only the % mucin binding. Studies indicated 8.33(%w/w) fraction of Pluronic F127 and 0.17 (%w/v) concentration of chitosan as optimum concentration. Finally, the optimized batch was characterized by polarized light microscopy, small-angle X-ray scattering (SAXS) and transmission electron microscopy. The results unveiled incorporation of chitosan did not disrupt the inner cubic structure of nanoparticles. Peak indexing of SAXS data revealed the coexistence of P-type and D-type cubic phases in nanoparticles. Further, comparative evaluation studies showed significantly higher anti-fungal activity of clotrimazole-loaded chitosan-tailored cubic nanoparticles than conventional suspension of clotrimazole against Candida albicans.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Verma P,Ahuja Mdoi
10.1016/j.ijbiomac.2014.10.065subject
Has Abstractpub_date
2015-02-01 00:00:00pages
138-45eissn
0141-8130issn
1879-0003pii
S0141-8130(14)00750-8journal_volume
73pub_type
杂志文章abstract::Alginate (ALg)-human-like collagen (HLC) microspheres were prepared by the technology of electrostatic droplet generation in order to develop a biocompatible vehicle for probiotic bacteria. Microparticles were spherical with mean particle size of 400μm. The encapsulation efficiency (EE) of ALg-HLC microspheres could r...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2011.08.018
更新日期:2011-12-01 00:00:00
abstract::Plasmin (EC 3.4.21.7) is a key enzyme of the fibrinolytic system, responsible for the degradation of fibrin clot and maintaining blood fluidity. Hence, alterations of the fibrinolytic capacity of blood plasma may contribute to thrombotic or bleeding complications. The aim of this study was to determine effects of a bu...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2018.09.143
更新日期:2018-12-01 00:00:00
abstract::Ubb+1, a ubiquitin (Ub) mutant protein originating from misreading of the Ub B gene, is found accumulated in brain tissues of Alzheimer's disease patients. The mutant attracts strong interest due to its possible participation in the molecular events leading to neurodegeneration. Ubb+1 is composed of the globular domai...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2017.11.121
更新日期:2018-03-01 00:00:00
abstract::The discovery of genes responsible for the production of bioactive metabolites via metabolic pathways combined with the advances in synthetic biology tools, has allowed the establishment of numerous microbial cell factories, for instance the yeast cell factories, for the manufacture of highly useful metabolites from r...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章,评审
doi:10.1016/j.ijbiomac.2018.01.149
更新日期:2018-06-01 00:00:00
abstract::Owing to the excellent cyto-compatibility of silk fibroin (SF) and the simple fabrication of nano-fibrous webs, electro-spun SF webs have attracted much research attention in numerous biomedical fields. Because the production rate of electro-spun webs is strongly dependent on the electro-spinning rate used, the electr...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2016.11.026
更新日期:2017-02-01 00:00:00
abstract::DNA vaccine is an important tool to elicit both humoral and cellular immunity. Present study investigates mucosal immune response of Labeo rohita (15 ± 04 g) to plasmid DNA (pDNA) vaccine macromolecule complexed with nanoparticles (NPs). Poly lactic-co-glycolic acid (PLGA), Chitosan (Chit) and PLGA-Chit-NPs were synth...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.04.048
更新日期:2020-08-01 00:00:00
abstract::Chitosan is a biodegradable, non-toxic, and antimicrobial polymer. Chitosan films can be used as a dressing because they promote the healing of cutaneous ulcers. In this study, the mechanical, physical, and microbiological properties films of pure chitosan and films formulated with a glycerol-honey mixture were charac...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2018.04.149
更新日期:2018-09-01 00:00:00
abstract::Ganoderma atrum polysaccharide (PSG-1), a main polysaccharide from Ganoderma atrum, possesses potent antioxidant capacity and cardiovascular benefits. The aim of this study was to investigate the role of PSG-1 in oxidative stress and apoptosis in human umbilical vein endothelial cells (HUVECs) under anoxia/reoxygenati...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2017.01.071
更新日期:2017-05-01 00:00:00
abstract::Three acidic polysaccharides (PPQA2, PPQA4 and PPQA5) were successfully purified from the water-extracted crude polysaccharides of Panax quinquefolius by combination of DEAE Sepharose and Sephacryl S-300 chromatography. The average molecular weights (Mws) of PPQA2, PPQA4 and PPQA5 were 2.3×10(4) Da, 1.2×10(5) Da, and ...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2015.06.007
更新日期:2015-09-01 00:00:00
abstract::A total of 18 protease producing bacterial strains were isolated from detergent effluent in South Korea using skim milk agar medium. A strain (BK-P21A) was selected and identified as Bacillus koreensis based on morphological, biochemical and molecular characterizations (16S rRNA gene sequence analysis). Optimized cult...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2013.02.014
更新日期:2013-05-01 00:00:00
abstract::Photo catalytic action of nano TiO(2) for decomposing of some organic compounds is a well known phenomenon. This can be extended to the application on nano TiO(2) on the desized cotton fabric to decompose the hydrophobic impurities and coloring matters of the fabric. This can be nominating as a replacement for the con...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2012.02.018
更新日期:2012-05-01 00:00:00
abstract::Debranching of (1, 6)-α-linkages in starch is of great significance as it is widely used in different industries. In recent years, identifying a single potential enzyme that could debranch (1, 6)-α-linkages and cleave (1, 4)-α-linkages brought great interest as amylopectin possessed both (1, 4)-α- and (1, 6)-α-linkage...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2017.03.129
更新日期:2017-08-01 00:00:00
abstract::Human serum albumin (HSA) contains three alpha-helical domains (I-III). The unfolding process of these domains was monitored using covalently bound fluorescence probes; domain I was monitored by N-(1-pyrene)maleimide (PM) conjugated with cys-34, domain II was monitored by the lone tryptophan residue and domain III was...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2005.10.009
更新日期:2005-12-15 00:00:00
abstract::Substantial amounts of industrial, agricultural, medical and domestic water worldwide are polluted by different types of contaminants. Removing these contaminants from effluent by adsorbent materials made from abundant, inexpensive polysaccharides is a feasible and promising approach to deal with this problem. In the ...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2017.03.091
更新日期:2017-08-01 00:00:00
abstract::The aim a present study was developed methylcellulose (MC) active edible films with extracts of green apple skin, as model systems of edible coating. Active edible films were developed by incorporation of ethanolic extract of freeze-dried apple skin (EEFD) and aqueous extract of apple skin (AES) at 10, 20 and 25% (v/v...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2018.12.114
更新日期:2019-03-01 00:00:00
abstract::Biopolymer used for the production of nanoparticles (NPs) has attracted increasing attention. In the presence article we use aqueous solution of polysaccharide Cyamopsis tetragonaloba commonly known as guar gum (GG), from plants. GG acts as reductive preparation of silver nanoparticles which are found to be <10 nm in ...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2012.06.033
更新日期:2012-11-01 00:00:00
abstract::Litchi polysaccharide (LP) was extracted from litchi pulp. Its chemical composition, microstructure, zeta potential, flow and viscoelastic behavior were investigated. LP contained uronic acid (41.18%), neutral sugar (42.23%), and protein (2.72%). The monosaccharide composition was mostly arabinose, galactose, and a sm...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2018.02.054
更新日期:2018-06-01 00:00:00
abstract::In the present study, small-sized porous scaffolds were obtained from the freeze-drying of sodium hyaluronate/chitosan polyelectrolyte complexes. The obtained materials were characterized by a set of techniques including attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy, swelling determin...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2011.06.011
更新日期:2011-11-01 00:00:00
abstract::The objectives of this paper were to investigate some physicochemical properties of Prunus armeniaca L. gum exudates (PAGE). PAGE had, on average, 66.89% carbohydrate, 10.47% uronic acids, 6.9% moisture (w.b.), 2.91% protein, 4% ash and 1.59% fat. PAGE was composed of monosaccharides including l-arabinose, d-galactose...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2015.09.068
更新日期:2016-01-01 00:00:00
abstract::Breast cancer endocrine resistance prevents unleashing full capabilities of Tamoxifen (TMX), besides TMX off-target side effects on healthy tissue. In this study, we engineered TMX nanocomposite via co-loading it on alginate-based silver nanoparticles and embedding within folic acid-polyethylene glycol surface conjuga...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2019.12.266
更新日期:2020-03-01 00:00:00
abstract::An effective method of free radical degradation induced by H2O2/ascorbic acid, which was different from previously reported H2O2/Cu2+ method, was developed to depolymerize a novel glycosaminoglycan from Holothuria mexicana (HmG). The results indicated that the degradation efficiency increased with the reduced solution...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2017.10.161
更新日期:2018-02-01 00:00:00
abstract::Removal behaviors of 152+154Eu, 60Co, and 134Cs radionuclides onto Chitosan-acrylic acid-1-vinyl-2-vinylpyrrolidone/oxidized multi-walled carbon nanotubes (CTS-AA-VP/o-MWCNTs) composite has been investigated by batch adsorption technique. CTS-AA-VP/o-MWCNTs composite has been synthesized by copolymerization of acrylic...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.08.120
更新日期:2020-12-01 00:00:00
abstract::In most of the recent research on emulsions related to food products containing protein-polysaccharide interactions established via the Maillard reaction have been used as emulsifiers. Key challenges in such studies include long reaction times, uncontrollable extent of reaction, and protein denaturation and aggregatio...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.09.007
更新日期:2020-12-01 00:00:00
abstract::Cationic starch nanoparticles were obtained by aqueous polyelectrolyte complex formation between cationic quaternary ammonium substituted starches and anionic sodium tripolyphosphate. The formation of nanosized starch particles of spherical shape was verified by dynamic light scattering and scanning electron microscop...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2012.01.037
更新日期:2012-04-01 00:00:00
abstract::The interaction of an amino-terminal-truncated 139 amino-acids form of human acidic fibroblast growth factor with myo-inositol hexasulphate and low molecular weight (3500 g mol(-1)) heparin has been studied by isothermal titration calorimetry, differential scanning calorimetry and Fourier transform infrared spectrosco...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/s0141-8130(01)00131-3
更新日期:2001-04-12 00:00:00
abstract::To explore new antioxidant resource from food, a water-soluble polysaccharide (ALP1) was extracted and purified from the roots of Arctium lappa L. (A. lappa L.) through hot water extraction followed by ethanol precipitation, ion-exchange chromatography and gel filtration. The antioxidant activity of ALP1 was then eval...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2014.01.062
更新日期:2014-04-01 00:00:00
abstract::Trace elements regulate many biological reactions in the body. Copper(II) is known as one of trace elements and capable of binding to proteins. Insulin is a blood glucose-lowering peptide hormone and it is secreted by the pancreatic β-cells. In this study, Cu(II)-insulin complexes were investigated by using ESI-MS met...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2018.01.150
更新日期:2018-06-01 00:00:00
abstract::Different biomass wastes were successfully blended with starch and Ecoflex® viz. poly(butylene adipate-co-terephthalate), without glycerol addition, to obtain biocomposite materials. The mechanical properties, as well as thermal and surface properties, of the developed composites were evaluated. It was found that the ...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2019.11.185
更新日期:2020-08-01 00:00:00
abstract::Critical-sized bone defects are augmented with cell free and cell loaded constructs to bridge bone defects. Improving the properties of three-dimensional scaffolds with multiple polymers and others is of growing interest in recent decades. Chitosan (CS), a natural biopolymer has limitations for its use in bone regener...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2017.01.034
更新日期:2017-11-01 00:00:00
abstract::Given the excellent characteristics of alginate, it is an industrially important polysaccharide. Mannuronan C5-epimerase (MC5E) is an alginate-modifying enzyme that catalyzes the conversion of β-D-mannuronate (M) to its C5 epimer α-L-guluronate (G) in alginate. Both the biological activities and physical properties of...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章,评审
doi:10.1016/j.ijbiomac.2020.11.123
更新日期:2021-01-31 00:00:00