Abstract：
:Molecular level, mechanistic understanding of the roles of reactive oxygen species (ROS) in a variety of pathological conditions is hindered by the difficulties associated with determining the concentration of various ROS species. Here, we present an approach that converts fold-change in the signal from an intracellular sensor of hydrogen peroxide into changes in absolute concentration. The method uses extracellular additions of peroxide and an improved biochemical measurement of the gradient between extracellular and intracellular peroxide concentrations to calibrate the intracellular sensor. By measuring peroxiredoxin activity, we found that this gradient is 650-fold rather than the 7-10-fold that is widely cited. The resulting calibration is important for understanding the mass-action kinetics of complex networks of redox reactions, and it enables meaningful characterization and comparison of outputs from endogenous peroxide generating tools and therapeutics across studies.
journal_name
Redox Bioljournal_title
Redox biologyauthors
Huang BK,Sikes HDdoi
10.1016/j.redox.2014.08.001subject
Has Abstractpub_date
2014-01-01 00:00:00pages
955-62issn
2213-2317pii
S2213-2317(14)00091-3journal_volume
2pub_type
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