Abstract:
BACKGROUND:Umbilical cord blood (UCB) has been used as an alternative source of donor hematopoietic stem cells for hematologic transplant setting over the past decade. This study attempted to evaluate potential predictors of cord blood quality. METHODS:A total of 750 UCB samples were studied (male, n = 365; female, n = 385). The impact of neonatal sex, weight and stromal cell-derived factor-1α polymorphism on the quality of these UCB samples was investigated. RESULTS:Male neonatal UCB was significantly richer in CD34(+) cells than was female UCB (P < 0.001), whereas female UCB was richer in total nucleated cells (P = 0.01). There was a slight correlation between CD34(+) cells concentration and UCB sample weight (P < 0.01) that could be attributed to the higher weight of male neonates. The use of tetra-polymerase chain reaction to detect stromal cell-derived factor-1α polymorphisms in 180 neonates revealed no differences between A/A, G/G and A/G allelic combinations. CONCLUSIONS:These data emphasize the lack of predictive factors for CD34(+) cells and total nucleated cell concentrations in UCB samples before processing.
journal_name
Cytotherapyjournal_title
Cytotherapyauthors
Bijou F,Ivanovic Z,Fizet D,Dazey B,Boiron JM,Lafarge Xdoi
10.1016/j.jcyt.2014.09.002subject
Has Abstractpub_date
2015-01-01 00:00:00pages
68-72issue
1eissn
1465-3249issn
1477-2566pii
S1465-3249(14)00784-1journal_volume
17pub_type
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