Affinity-based methods in drug-target discovery.

Abstract:

:Target discovery using the molecular approach, as opposed to the more traditional systems approach requires the study of the cellular or biological process underlying a condition or disease. The approaches that are employed by the "bench" scientist may be genetic, genomic or proteomic and each has its rightful place in the drug-target discovery process. Affinity-based proteomic techniques currently used in drug-discovery draw upon several disciplines, synthetic chemistry, cell-biology, biochemistry and mass spectrometry. An important component of such techniques is the probe that is specifically designed to pick out a protein or set of proteins from amongst the varied thousands in a cell lysate. A second component, that is just as important, is liquid-chromatography tandem massspectrometry (LC-MS/MS). LC-MS/MS and the supporting theoretical framework has come of age and is the tool of choice for protein identification and quantification. These proteomic tools are critical to maintaining the drug-candidate supply, in the larger context of drug discovery.

journal_name

Curr Drug Targets

journal_title

Current drug targets

authors

Rylova G,Ozdian T,Varanasi L,Soural M,Hlavac J,Holub D,Dzubak P,Hajduch M

doi

10.2174/1389450115666141120110323

subject

Has Abstract

pub_date

2015-01-01 00:00:00

pages

60-76

issue

1

eissn

1389-4501

issn

1873-5592

pii

CDT-EPUB-63480

journal_volume

16

pub_type

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