Abstract:
:Breast cancer heterogeneity demands that prognostic models must be biologically driven and recent clinical evidence indicates that future prognostic signatures need evaluation in the context of early compared with late metastatic risk prediction. In pre-clinical studies, we and others have shown that various protein-protein interactions, pertaining to the actin microfilament-associated proteins, ezrin and cofilin, mediate breast cancer cell migration, a prerequisite for cancer metastasis. Moreover, as a direct substrate for protein kinase Cα, ezrin has been shown to be a determinant of cancer metastasis for a variety of tumour types, besides breast cancer; and has been described as a pivotal regulator of metastasis by linking the plasma membrane to the actin cytoskeleton. In the present article, we demonstrate that our tissue imaging-derived parameters that pertain to or are a consequence of the PKC-ezrin interaction can be used for breast cancer prognostication, with inter-cohort reproducibility. The application of fluorescence lifetime imaging microscopy (FLIM) in formalin-fixed paraffin-embedded patient samples to probe protein proximity within the typically <10 nm range to address the oncological challenge of tumour heterogeneity, is discussed.
journal_name
Biochem Soc Transjournal_title
Biochemical Society transactionsauthors
Weitsman G,Lawler K,Kelleher MT,Barrett JE,Barber PR,Shamil E,Festy F,Patel G,Fruhwirth GO,Huang L,Tullis ID,Woodman N,Ofo E,Ameer-Beg SM,Irshad S,Condeelis J,Gillett CE,Ellis PA,Vojnovic B,Coolen AC,Ng Tdoi
10.1042/BST20140165subject
Has Abstractpub_date
2014-12-01 00:00:00pages
1498-505issue
6eissn
0300-5127issn
1470-8752pii
BST20140165journal_volume
42pub_type
杂志文章,评审abstract::The transcript is populated with numerous overlapping codes that regulate all steps of gene expression. Deciphering these codes is very challenging due to the large number of variables involved, the non-modular nature of the codes, biases and limitations in current experimental approaches, our limited knowledge in gen...
journal_title:Biochemical Society transactions
pub_type: 杂志文章
doi:10.1042/BST20191048
更新日期:2020-08-28 00:00:00
abstract::Zebrafish (Danio rerio) are becoming an increasingly powerful model organism to study the role of metabolism in disease. Since its inception, the zebrafish model has relied on unique attributes such as the transparency of embryos, high fecundity and conservation with higher vertebrates, to perform phenotype-driven che...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/BST20180335
更新日期:2019-02-28 00:00:00
abstract::Mutations in MYO7A (myosin VIIa) cause Usher syndrome type 1B, a disorder involving profound congenital deafness and progressive blindness. In the retina, most MYO7A is localized in the apical region of the RPE (retinal pigmented epithelial) cells, and a small amount is associated with the ciliary and periciliary memb...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/BST0391207
更新日期:2011-10-01 00:00:00
abstract::We have investigated serum chemokines for their suitability as markers of atherosclerosis development in apoE (apolipoprotein E)-deficient ((-/-)) mice. Female C3H apoE(-/-) and C57BL apoE(-/-) mice were fed on either diet W (Western diet; 6 weeks) or normal rodent diet (12 weeks). Serum lipids (0, 6 and 12 weeks) and...
journal_title:Biochemical Society transactions
pub_type: 杂志文章
doi:10.1042/bst0320128
更新日期:2004-02-01 00:00:00
abstract::Over the last 10 years, during the lifetime of the nitrogen cycle meetings, structural biology, coupled with spectroscopy, has had a major impact of our understanding enzymology of the nitrogen cycle. The three-dimensional structures for many of the key enzymes have now been resolved and have provided a wealth of info...
journal_title:Biochemical Society transactions
pub_type: 杂志文章
doi:10.1042/BST0330113
更新日期:2005-02-01 00:00:00
abstract::The examples shown here illustrate the power of gene targeting to the epidermis as a means of developing animal models for the study of human skin diseases. In this short review, I have focused on contributions which stem predominantly from my own laboratory [31, 32, 34, 37, 47]. However, other laboratories have also ...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/bst0191112
更新日期:1991-11-01 00:00:00
abstract::Among the several signalling pathways regulated by ubiquitin and ubiquitin-like proteins, the one activating NF-kappaB (nuclear factor kappaB) is certainly one of the best characterized. The regulation of the activity of this transcription factor by members of the ubiquitin family occurs at various levels, imposing ov...
journal_title:Biochemical Society transactions
pub_type: 杂志文章
doi:10.1042/BST0360853
更新日期:2008-10-01 00:00:00
abstract::Bacterial hydrogenases have been harnessed to the removal of heavy metals from solution by reduction to less soluble metal species. For Pd(II), its bioreduction results in the deposition of cell-bound Pd(0)-nanoparticles that are ferromagnetic and have a high catalytic activity. Hydrogenases can also be used synthetic...
journal_title:Biochemical Society transactions
pub_type: 杂志文章
doi:10.1042/BST0330076
更新日期:2005-02-01 00:00:00
abstract::The discovery of the zDHHC family of S-acyltransferase enzymes has been one of the major breakthroughs in the S-acylation field. Now, more than a decade since their discovery, major questions centre on profiling the substrates of individual zDHHC enzymes (there are 24 ZDHHC genes and several hundred S-acylated protein...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/BST20140270
更新日期:2015-04-01 00:00:00
abstract::The pathogenic Neisseria have a siderophore-independent iron-uptake system reliant on a direct interaction between the bacterial cell and transferrin. In the meningococcus this uptake system is dependent on two surface-exposed transferrin-binding proteins. This short account will review our current knowledge of the tr...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/bst0300705
更新日期:2002-08-01 00:00:00
abstract::The production of dihydrogen by Escherichia coli and other members of the Enterobacteriaceae is one of the classic features of mixed-acid fermentation. Synthesis of the multicomponent, membrane-associated FHL (formate hydrogenlyase) enzyme complex, which disproportionates formate into CO(2) and H(2), has an absolute r...
journal_title:Biochemical Society transactions
pub_type: 杂志文章
doi:10.1042/BST0330042
更新日期:2005-02-01 00:00:00
abstract::RNA pseudoknots are structural elements found in almost all classes of RNA. Pseudoknots form when a single-stranded region in the loop of a hairpin base-pairs with a stretch of complementary nucleotides elsewhere in the RNA chain. This simple folding strategy is capable of generating a large number of stable three-dim...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/BST0360684
更新日期:2008-08-01 00:00:00
abstract::Assembly of ribonucleoprotein complexes is a facilitated quality-controlled process that typically includes modification to the RNA component from precursor to mature form. The SRP (signal recognition particle) is a cytosolic ribonucleoprotein that catalyses protein targeting to the endoplasmic reticulum. Assembly of ...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/BST0381093
更新日期:2010-08-01 00:00:00
abstract::Cytochrome P450 (CYP) enzymes represent a large superfamily that displays extraordinarily diverse substrate specificities. After a concise review about CYPs of the CYP1A subfamily, which plays a crucial role in procarcinogen activation, this paper presents segment-directed mutagenesis. This approach generates a librar...
journal_title:Biochemical Society transactions
pub_type: 杂志文章
doi:10.1042/0300-5127:0290128
更新日期:2001-05-01 00:00:00
abstract::The progression of Type 2 diabetes is accompanied by diminishing islet β-cell mass and function. It has been proposed that β-cells are lost not only through apoptosis, but also by dedifferentiating into progenitor-like cells. There is therefore much interest in the mechanisms which define and maintain β-cell identity....
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/BST20120355
更新日期:2013-06-01 00:00:00
abstract::Dynein is an AAA+ (ATPase associated with various cellular activities)-type motor complex that utilizes ATP hydrolysis to actively drive microtubule sliding. The dynein heavy chain (molecular mass >500 kDa) contains six tandemly linked AAA+ modules and exhibits full motor activities. Detailed molecular dissection of t...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/BST0360131
更新日期:2008-02-01 00:00:00
abstract::Isolation and purification of the [NiFe] hydrogenase of Desulfovibrio vulgaris Miyazaki F under aerobic conditions leads to a mixture of two states, Ni-A (unready) and Ni-B (ready). The two states are distinguished by different activation times and different EPR spectra. HYSCORE and ENDOR data and DFT calculations sho...
journal_title:Biochemical Society transactions
pub_type: 杂志文章
doi:10.1042/BST0330007
更新日期:2005-02-01 00:00:00
abstract::TDP-43 (TAR DNA-binding protein 43) is an hnRNP (heterogeneous nuclear ribonucleoprotein) protein whose role in cellular processes has come to the forefront of neurodegeneration research after the observation that it is the main component of brain inclusions in ALS (amyotrophic lateral sclerosis) and FTLD (frontotempo...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/BST20130186
更新日期:2013-12-01 00:00:00
abstract::A flavorubredoxin and its associated oxidoreductase (encoded by norV and norW respectively) detoxify NO (nitric oxide) to form N2O (nitrous oxide) under anaerobic conditions in Escherichia coli. Transcription of the norVW genes is activated in response to NO by the σ54-dependent regulator and dedicated NO sensor, NorR...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/BST0390289
更新日期:2011-01-01 00:00:00
abstract::Higher eukaryotes rely on AS (alternative splicing) of pre-mRNAs (mRNA precursors) to generate more than one protein product from a single gene and to regulate mRNA stability and translational activity. An important example of the latter function involves an interplay between AS and NMD (nonsense-mediated decay), a cy...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/BST20140066
更新日期:2014-08-01 00:00:00
abstract::Nitrogenase activity in Rhodospirillum rubrum is post-translationally regulated by DRAG (dinitrogenase reductase glycohydrolase) and DRAT (dinitrogenase reductase ADP-ribosylation transferase). When a sudden increase in fixed nitrogen concentration or energy depletion is sensed by the cells, DRAG is inactivated and DR...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/BST0340160
更新日期:2006-02-01 00:00:00
abstract::Lipids are key molecules for membranes, energy storage and signalling. I have been privileged to have worked in such a diverse field and in organisms from microbes to humans. Here I will describe some of those contrasting areas which range from environmental impacts to food production and on to human health. It has be...
journal_title:Biochemical Society transactions
pub_type: 历史文章,杂志文章,评审
doi:10.1042/BST20160406
更新日期:2017-04-15 00:00:00
abstract::Chromosomal instability occurs early in the development of cancer and may represent an important step in promoting the multiple genetic changes required for the initiation and/or progression of the disease. Telomere erosion is one of the factors that contribute to chromosome instability through end-to-end chromosome f...
journal_title:Biochemical Society transactions
pub_type: 杂志文章
doi:10.1042/BST0381698
更新日期:2010-12-01 00:00:00
abstract::A considerable number of fungal homologues of human apoptotic genes have been identified in recent years. Nevertheless, we are far from being able to connect the different pieces and construct a primary structure of the fungal apoptotic regulatory network. To get a better picture of the available fungal components, we...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/BST0391493
更新日期:2011-10-01 00:00:00
abstract::Data from experimental studies in animals and from epidemiological studies in humans suggest a link between insulin and cognitive performance. Do these results translate into clinical and therapeutic benefit for people with cognitive impairment? Insulin injected peripherally can readily cross the blood-brain barrier. ...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/BST20051037
更新日期:2005-11-01 00:00:00
abstract::Cell-free transcription-translation is an expanding field in synthetic biology as a rapid prototyping platform for blueprinting the design of synthetic biological devices. Exemplar efforts include translation of prototype designs into medical test kits for on-site identification of viruses (Zika and Ebola), while gene...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/BST20170011
更新日期:2017-06-15 00:00:00
abstract::Within the SulfoSYS (Sulfolobus Systems Biology) project, the effect of temperature on a metabolic network is investigated at the systems level. Sulfolobus solfataricus utilizes an unusual branched ED (Entner-Doudoroff) pathway for sugar degradation that is promiscuous for glucose and galactose. In the course of metab...
journal_title:Biochemical Society transactions
pub_type: 杂志文章
doi:10.1042/BST0390077
更新日期:2011-01-01 00:00:00
abstract::Findings from gene expression profiling studies are leading to new diagnostic and therapeutic strategies that can be applied in medical practice, especially in the field of oncology. Promising results of gene expression profiling of the peripheral blood in patients with ischaemic stroke have been obtained in recent pi...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/BST0341313
更新日期:2006-12-01 00:00:00
abstract::The revolution of genomics and growth of systems biology urged the creation of synthetic biology, an engineering discipline aiming at recreating and reprogramming cellular functions for industrial needs. There has been a huge effort in synthetic biology to develop versatile and programmable genetic regulators that wou...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/BST20190173
更新日期:2019-10-31 00:00:00
abstract::Our first experience of mannan-binding lectin (MBL)-replacement therapy was with a patient experiencing recurrent erythema multiforme associated with reactivation of herpes simplex virus; his erythematous eruptions could be controlled with infusions of fresh frozen plasma containing MBL, but not with plasma lacking MB...
journal_title:Biochemical Society transactions
pub_type: 杂志文章
doi:10.1042/bst0310768
更新日期:2003-08-01 00:00:00