Abstract:
:Type-III protein secretion systems are utilized by gram-negative pathogens to secrete building blocks of the bacterial flagellum, virulence effectors from the cytoplasm into host cells, and structural subunits of the needle complex. The flagellar type-III secretion apparatus utilizes both the energy of the proton motive force and ATP hydrolysis to energize substrate unfolding and translocation. We report formation of functional flagella in the absence of type-III ATPase activity by mutations that increased the proton motive force and flagellar substrate levels. We additionally show that increased proton motive force bypassed the requirement of the Salmonella pathogenicity island 1 virulence-associated type-III ATPase for secretion. Our data support a role for type-III ATPases in enhancing secretion efficiency under limited secretion substrate concentrations and reveal the dispensability of ATPase activity in the type-III protein export process.
journal_name
PLoS Genetjournal_title
PLoS geneticsauthors
Erhardt M,Mertens ME,Fabiani FD,Hughes KTdoi
10.1371/journal.pgen.1004800subject
Has Abstractpub_date
2014-11-13 00:00:00pages
e1004800issue
11eissn
1553-7390issn
1553-7404pii
PGENETICS-D-14-01838journal_volume
10pub_type
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