Effect of HGF on the apoptosis of rat corpus cavernosum smooth muscle cells induced by TGFβ1.


:Corpus cavernosum smooth muscle cells (CCSMCs) are important functional cells for penile erection. We evaluated the effect of transforming growth factor β1 (TGFβ1) and hepatocyte growth factor (HGF) on the viability and apoptosis of CCSMCs in vitro. CCSMCs from healthy male Sprague Dawley rats were randomly divided into four groups: a negative control group, a TGFβ1 group, a HGF group and a HGF+ TGFβ1 group. Differences in cell viability and apoptosis among groups were observed by 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay and flow cytometry. Western blot was used to detect the change of apoptosis-related proteins. The level of reactive oxygen species (ROS) was detected by colorimetry. In the TGFβ1 group, the MTT values were obviously decreased at 12 h, 24 h, 48 h-0.320, 0.383 and 0.432 respectively. However, compared with the normal group, the apoptosis index was markedly increased, reaching 26.86% at the 48-h time point. After TGFβ1 treatment, the levels of cleaved caspase-3 and p-Smad2 were increased in the cells, but the levels of Bcl-xL, Bcl-2 and p-Akt were significantly lower. However, HGF co-treatment partially reversed these changes and could decrease the intracellular ROS level while increasing the Akt phosphorylation level. These results indicate that TGFβ1 might induce apoptosis of CCSMCs in vitro and that HGF could interfere with the above process through downregulation of apoptosis signalling and oxidative stress reaction.






Liu T,Peng YF,Jia C,Yang BH,Tao X,Fang X,Zhong W




Has Abstract


2015-11-01 00:00:00












  • Decreased prostate-specific membrane antigen levels in the seminal plasma of oligoasthenoteratozoospermic men.

    abstract::Prostate-specific membrane antigen (PSMA) is a transmembrane glycoprotein with glutamate carboxypeptidase activity. However, its precise function in the prostate, prostasomes and seminal plasma with regard to male fertility remains unknown. This study was conducted to investigate the seminal plasma PSMA levels in fert...


    pub_type: 杂志文章


    authors: Arslan MA,Avcı B,Tunçel ÖK,Asci R

    更新日期:2021-02-01 00:00:00

  • NGF promotes mitochondrial function by activating PGC-1α in TM4 Sertoli cells.

    abstract::Nerve growth factor (NGF), which is required for the survival and differentiation of the nervous system, has been proved to play important roles in male reproductive physiology. Several studies have focused on the roles of NGF in the testes. However, no study has reported on the mechanism of paracrine and autocrine ac...


    pub_type: 杂志文章


    authors: Jiang YW,Zhao Y,Chen SX,Chen L,Li CJ,Zhou X

    更新日期:2018-02-01 00:00:00

  • Nonarteritic ischaemic optic neuropathy (NAION) after 36 h of intake of sildenafil citrate: first Egyptian case.

    abstract::Sildenafil citrate is a selective phosphodiesterase 5 (PDE-5) inhibitor and partial phosphodiesterase 6 inhibitor prescribed for erectile dysfunction. Post-marketing case reports of nonarteritic anterior ischaemic optic neuropathy (NAION) over the past few years suggest a potential link with PDE-5 inhibitors. We repor...


    pub_type: 杂志文章


    authors: El-Domyati MM,El-Fakahany HM,Morad KE

    更新日期:2009-10-01 00:00:00

  • The current perspective on genetic and epigenetic factors in sperm maturation in the epididymis.

    abstract::Male infertility affects approximately 30% of infertile couples. As spermatozoa mature in the epididymal lumen, their potential for mobility increases, and their protein, lipid and small RNA (sRNA) content changes, whereas capacitation and fertilisation take place in the female reproductive tract. Both of the latter p...


    pub_type: 杂志文章,评审


    authors: Ozkocer SE,Konac E

    更新日期:2021-01-25 00:00:00

  • Semen quality in adult male survivors 5 years after the 2008 Wenchuan earthquake.

    abstract::The influence of the Wenchuan earthquake on semen quality of adult male survivors is unclear. We investigated the semen quality included 673 male survivors from the worse-affected counties in the earthquake between Aug 2008 and July 2013. Semen parameters including pH, volume, concentration, motility and morphology we...


    pub_type: 杂志文章


    authors: Chen XM,Chen SM,Yue HX,Lin L,Wu YB,Liu B,Jiang M,Ma YX

    更新日期:2016-12-01 00:00:00

  • Bovine serum albumin: survival and osmolarity effect in bovine spermatozoa stored above freezing point.

    abstract::Liquid nitrogen preservation in remote farms is a limitation. The goal of this study was to determine optimum temperature above freezing point for bovine spermatozoa preservation using bovine serum albumin (BSA) as a supplementation. Pooled semen sample from three ejaculates was subjected to various BSA concentration ...


    pub_type: 杂志文章


    authors: Nang CF,Osman K,Budin SB,Ismail MI,Jaffar FH,Mohamad SF,Ibrahim SF

    更新日期:2012-05-01 00:00:00

  • Testicular function and prolactin responsiveness to TRH and cimetidine after renal transplantation.

    abstract::The hypothalamic-pituitary-testicular axis and the regulation of prolactin secretion were investigated in eleven male renal transplant recipients. Mean serum levels of testosterone and estrone were normal, whereas those of androstenedione and estradiol were low. Mean basal luteinizing hormone (LH) levels were slightly...


    pub_type: 杂志文章


    authors: De Besi L,Serafini E,Gasparotto ML,Mastrogiacomo I

    更新日期:1988-03-01 00:00:00

  • Disordered APC/C-mediated cell cycle progression and IGF1/PI3K/AKT signalling are the potential basis of Sertoli cell-only syndrome.

    abstract::The cause of Sertoli cell-only syndrome (SCOS), a condition in which only Sertoli cells line the seminiferous tubules in the testis, is unknown. Three microarray data sets were downloaded from public databases and were used to compare SCOS and control group. A total of 291 genes differentially expressed (Log2 |FC| ≥ 1...


    pub_type: 杂志文章


    authors: Zhang H,Zhou D,Zhu F,Chen F,Zhu Y,Yu R,Fan L

    更新日期:2019-08-01 00:00:00

  • A study on the association between serum amyloid A and sperm concentration.

    abstract::Our aim was to compare peripheral blood and seminal fluid serum amyloid A (SAA) protein levels in men classified on the basis of sperm concentration and investigate whether SAA protein is an important marker of male infertility. A total of 74 first-attempt IVF male partners of infertile couples classified as azoosperm...


    pub_type: 杂志文章


    authors: Timur H,Kokanali MK,Inal HA,Tuzluoglu D,Yilmaz N

    更新日期:2016-08-01 00:00:00

  • Preliminary observations on steroid metabolism in isolated epithelum of guinea pig seminal vesicle.

    abstract::The metabolism of nine radioactively labeled steroids in the epithelium of the seminal vesicle of the mature guinea pig has been studied. The rapid assimilation and metabolism of these steroids demostrate the very active biochemical nature of this tissue. Based on the use of several thin-layer chromatography systems a...


    pub_type: 杂志文章


    authors: Steer RC,Veneziale CM

    更新日期:1977-04-01 00:00:00

  • Chronic ethanol intake may delay the onset of gossypol-induced infertility in the male rat.

    abstract::Parameters were obtained from the reproductive organs of ethanol-fed, gossypol-treated Sprague Dawley rats. The experimental animals were fed either on a normal (15.17%) or low protein (8.00%) diet. Measurements included reproductive organ weights, seminal characteristics, serum concentration of testosterone and histo...


    pub_type: 杂志文章


    authors: Akingbemi BT,Rao PV,Aire TA

    更新日期:1997-07-01 00:00:00

  • Glutathione S-transferase Mu-1 gene polymorphism in Egyptian patients with idiopathic male infertility.

    abstract::The aim of this study was to examine whether an association exists between glutathione S-transferase Mu-1 (GSTM1) gene polymorphism and idiopathic male infertility. Sixty men with primary idiopathic infertility and 60 fertile men, serving as controls, were recruited for the study. The polymorphism was analysed using p...


    pub_type: 杂志文章


    authors: Roshdy OH,Hussein TM,Zakaria NH,Sabry AA

    更新日期:2015-06-01 00:00:00

  • Ethanol-induced mitophagy in rat Sertoli cells: Implications for male fertility.

    abstract::Autophagy is a pro-survival mechanism involving lysosomal degradation of damaged cellular components following multiple forms of cellular stress. There is currently a lack of literature on the mechanism, and specifically on mitophagy (selective autophagy of damaged pro-apoptotic mitochondria) in Sertoli cells (SCs). A...


    pub_type: 杂志文章


    authors: Eid N,Kondo Y

    更新日期:2018-02-01 00:00:00

  • Effects of two-year testosterone replacement therapy on cognition, emotions and quality of life in young and middle-aged hypogonadal men.

    abstract::The aim of the study was to examine the effects of two-year testosterone replacement therapy on cognitive functioning, emotional state and quality of life in young and middle-aged men with hypogonadotropic hypogonadism. Nineteen males diagnosed with hypogonadotropic hypogonadism participated in the study. Cognitive fu...


    pub_type: 临床试验,杂志文章


    authors: Lašaitė L,Čeponis J,Preikša RT,Žilaitienė B

    更新日期:2017-04-01 00:00:00

  • Study of sperm morphologic characteristics in a group of 833 fertile men.

    abstract::For the 13 abnormalities under consideration, the mean percentages were found to be the following: irregular head (15.2), microcephalic (9.8), elongated head (5.9), coiled tail (5.8), bent tail (4.7), thin head (4.6), cytoplasmic remains (3.2), macrocephalic (1.6), short tail (1.1), no tail (0.6), duplicate tail (0.5)...


    pub_type: 杂志文章


    authors: Schwartz D,Mayaux MJ,Guihard-Moscato ML,Spira A,Jouannet P,Czyglik F,David G

    更新日期:1984-09-01 00:00:00

  • The Estonian version of the National Institutes of Health chronic prostatitis symptom index.

    abstract::Our aim was to establish the Estonian version of the National Institutes of Health chronic prostatitis symptom index (NIH-CPSI), and to examine its validity and applicability in chronic prostatitis patients as well as in community-based study. The questionnaire was tested in 54 category III prostatitis patients. In ad...


    pub_type: 杂志文章


    authors: Korrovits P,Punab M,Mehik A,Mändar R

    更新日期:2006-06-01 00:00:00

  • Association of IgA secretory component sialylation with leucocytospermia of infertile men - a pilot study.

    abstract::The aim of our pilot study was to check whether the differences in IgA secretory component (SC) sialylation are associated with leucocytospermia. In normozoospermic and leucocytospermic seminal plasmas, 78-kDa and 63-kDa SC immunoreactive bands were observed. The SC sialylation was analysed by lectin blotting, using s...


    pub_type: 杂志文章


    authors: Kratz EM,Ferens-Sieczkowska M

    更新日期:2014-12-01 00:00:00

  • Direct measurement of the tail beat frequency of human sperm by flash light synchronization.

    abstract::Based on the synchronization between a periodic vibration and flashing light, we designed a device to determine the flagellar beating frequency of human sperm. The head of a spermatozoon was either held by the tip of a micropipette operated with a micromanipulator or adhered by itself on the surface of a glass slide w...


    pub_type: 杂志文章


    authors: Chen CS,Chao HT,Leng CH,Pan RL,Wei YH

    更新日期:1998-02-01 00:00:00

  • Blueberries prevent the effect of intermittent hypobaric hypoxia in rat epididymis.

    abstract::Intermittent hypobaric hypoxia (IHH) induced a decrease in sperm count and oxidative damage in epididymis. We have previously demonstrated that a blueberry-enriched polyphenol extract (BB-4) reduced the adverse effects of oxidative stress in rat testis under hypobaric hypoxia. The aim of this study was to evaluate whe...


    pub_type: 杂志文章


    authors: Zepeda AB,Calaf GM,Figueroa CA,Farías JG

    更新日期:2014-09-01 00:00:00

  • Electrophoretic characterization of mouse epididymal esterases in inbred lines and in a natural population.

    abstract::Esterase isozymes were studied in mouse epididymis of two inbred strains (C57BL, DBA/2) and in a natural population (Swiss OF1), by using vertical polyacrylamide gel electrophoresis and staining with alpha or beta-naphthyl acetate as a substrate. Eighteen (C57BL), 17 (DBA/2) or 16 (Swiss OF1) epididymal isozymes were ...


    pub_type: 杂志文章


    authors: Abou-Haila A,Fain-Maurel MA

    更新日期:1986-11-01 00:00:00

  • Dopamine D2 receptors in the basolateral amygdala modulate erectile function in a rat model of nonorganic erectile dysfunction.

    abstract::Nonorganic erectile dysfunction is a problem with unknown central mechanisms. Changes in brain activity in the amygdala have been observed in human patients. This study aimed to investigate the dopamine system in the basolateral amygdala of male rats with nonorganic erectile dysfunction. We applied chronic mild stress...


    pub_type: 杂志文章


    authors: Chen G,Chen J,Yang B,Yu W,Chen Y,Dai Y

    更新日期:2019-02-01 00:00:00

  • Genetic analysis of the human Insulin-like 3 gene: absence of mutations in a Greek paediatric cohort with testicular maldescent.

    abstract::This study investigated the hypothesis that genetic alterations of the human insulin-like 3 (INSL3) gene are associated with testicular maldescent (TMD). Genomic DNA was extracted and amplified from peripheral blood samples of 170 unrelated children with all possible phenotypical expressions of TMD and 50 volunteers w...


    pub_type: 杂志文章,评审


    authors: Mamoulakis C,Georgiou I,Dimitriadis F,Tsounapi P,Giannakis I,Chatzikyriakidou A,Antypas S,Sofras F,Takenaka A,Sofikitis N

    更新日期:2014-01-01 00:00:00

  • Antiapoptotic effects of dehydroepiandrosterone on testicular torsion/detorsion in rats.

    abstract::In the present study, we aimed to evaluate the effects of dehydroepiandrosterone (DHEA) on apoptosis of testicular germ cells after repair of testicular torsion in rats. Twenty-four adult male Sprague-Dawley rats were randomly divided into four groups, with six rats in each group: sham operation, torsion/detorsion (T/...


    pub_type: 杂志文章


    authors: Yapanoglu T,Aksoy Y,Gursan N,Ozbey I,Ziypak T,Calik M

    更新日期:2008-02-01 00:00:00

  • Adequacy of hyaluronan binding assay and a new fertility index derived from it for measuring of male fertility potential and the efficacy of supplement therapy.

    abstract::The aim of the study was to statistically prove that the HBA(®) test is an efficient practical method for andrologists to determine the fertility potential as well as to measure the efficiency of oral supplement therapy in case of male infertility. In the study, 175 patients were involved and it also included the foll...


    pub_type: 杂志文章


    authors: Szucs M,Osvath P,Laczko I,Jakab A

    更新日期:2015-06-01 00:00:00

  • Acid-extracted glycoproteins in human seminal coagulum.

    abstract::Protein in intact coagulum of fresh human ejaculate was extracted by 0.25 N HCl, the condition used for extraction of sperm basic proteins. About 75% of the total coagulum protein was found to be acid-extractable and therefore so-called coagulum basic proteins (CBP). The CBP were glycosylated and entirely distinguisha...


    pub_type: 杂志文章


    authors: Kusamran T,Surakarnkul R

    更新日期:1983-07-01 00:00:00

  • Assessment of sperm function in fertile and infertile men.

    abstract::The sperm function of fertile men (control), infertility patients (experimental), and men with varicocele were compared. The bioassays used were the follicular fluid-induced acrosome reaction, the binding to the zona pellucida, and the penetration of zona-free hamster oocytes. The percentage (mean +/- SEM) of reacted ...


    pub_type: 杂志文章


    authors: Vigil P,Wöhler C,Bustos-Obregón E,Comhaire F,Morales P

    更新日期:1994-03-01 00:00:00

  • Tramadol (opioid) abuse is associated with a dose- and time-dependent poor sperm quality and hyperprolactinaemia in young men.

    abstract::Tramadol, one of the most commonly abused drugs in Middle East, impacts spermatogenesis and disturbs reproductive hormones in animal studies. We aimed to investigate tramadol impact on sperm quality and on levels of testosterone, prolactin and gonadotropins, in tramadol abusers (n = 30) to age-matched control (n = 30)...


    pub_type: 杂志文章


    authors: Farag AGA,Basha MA,Amin SA,Elnaidany NF,Elhelbawy NG,Mostafa MMT,Khodier SA,Ibrahem RA,Mahfouz RZ

    更新日期:2018-08-01 00:00:00

  • Acute bacterial prostatitis and chronic prostatitis/chronic pelvic pain syndrome: andrological implications.

    abstract::There is a consensus on the diagnostic management of bacterial prostatitis (acute and chronic). In chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) the diagnostic approach remains unclear, because inflammatory and noninflammatory CP/CPPS might be one entity with varying findings over time. The WHO definition...


    pub_type: 杂志文章,评审


    authors: Weidner W,Wagenlehner FM,Marconi M,Pilatz A,Pantke KH,Diemer T

    更新日期:2008-04-01 00:00:00

  • Serum sperm agglutinating antibody formation in vasectomized men treated with dexamethasone.

    abstract::The effect of immunosuppression with dexamethasone on sperm antibody formation following vasectomy in men was determined. Vasectomized men were treated with dexamethasone (4 mg) tablets starting two days prior to vasectomy and each day thereafter for a total of eight days for a total dose of 30 mg. There was no signif...


    pub_type: 临床试验,杂志文章


    authors: Curtis GL,Kahl VA,Ryan WL

    更新日期:1987-11-01 00:00:00

  • Cyproterone acetate and a small dose of oestrogen in the pre-operative management of male transsexuals. A report of three cases.

    abstract::The pre-operative treatment of three adult male transsexuals with the anti-androgen and progestogenic compound cyproterone acetate is reported. When used in combination with cyproterone acetate, only a very small dose of oestrogen is needed to induce feminisation. The effect of this treatment on both testicular and ep...


    pub_type: 杂志文章


    authors: Jequier AM,Bullimore NJ,Bishop MJ

    更新日期:1989-09-01 00:00:00