Abstract：
:Saccharomyces cerevisiae strains vary in their ability to develop and enhance sensory attributes of alcoholic beverages and are often found growing in mixed strain fermentations; however, quantifying individual strains is challenging due to quantification inaccuracies, low marker longevity, and compromised kinetics. We developed a fluorescent probe, consisting of glutathione molecules conjugated to a quantum dot (QD). Two S. cerevisiae strains were incubated with different coloured probes (QD attached to glutathione molecules, QD-GSH), fermented at multiple ratios, and quantified using confocal microscopy. The QD method was compared with a culture method using microsatellite DNA analysis (MS method). Probes were taken up by an ADP1 encoded transporter, transferred from mother cell to daughter cell, detectable in strains throughout fermentation, and were non-toxic. This resulted in a new quantification method that was more accurate and efficient than the MS method.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Gustafsson FS,Whiteside MD,Jiranek V,Durall DMdoi
10.1038/srep06971subject
Has Abstractpub_date
2014-11-10 00:00:00pages
6971issn
2045-2322pii
srep06971journal_volume
4pub_type
杂志文章abstract：:At present, there is a growing interest in the study of the neurotropic activity of polyunsaturated fatty acids ethanolamides (N-acylethanolamines). N-docosahexaenoylethanolamine (DHEA, synaptamide) is an endogenous metabolite and structural analogue of anandamide, a widely studied endocannabinoid derived from arachid...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-80818-9
更新日期：2021-01-12 00:00:00
abstract：:This study aimed to determine the performance and long-term outcomes of therapeutic ERCP in very old patients. Patients aged or over 90 (Group A, n = 78) and consecutive sex-matched controls (Group B, n = 312) under 65 selected were compared. More patients in Group A had chronic concomitant diseases, but the success a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep04918
更新日期：2014-05-13 00:00:00
abstract：:Respiratory syncytial virus (RSV) is a significant cause of lower respiratory tract infection in infants and elderly. To understand the evolution of neutralizing epitopes on the RSV glycoprotein (G) and fusion (F) proteins, we conducted a multi-year surveillance program (OUTSMART-RSV) in the US. Analysis of 1,146 RSV ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-40387-y
更新日期：2019-03-07 00:00:00
abstract：:Novel-morphological Fe3O4 nanosheets with magnetochromatic property have been prepared by a modified solvothermal method. Such nanosheets could form one-dimension photonic crystal under an external magnetic field. The Fe3O4 nanosheets suspension could strongly diffract visible light and display varied colors with chan...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep09320
更新日期：2015-03-23 00:00:00
abstract：:Healthy sleep, positive general affect, and the ability to regulate emotional experiences are fundamental for well-being. In contrast, various mental disorders are associated with altered rapid eye movement (REM) sleep, negative affect, and diminished emotion regulation abilities. However, the neural processes mediati...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-74169-8
更新日期：2020-10-14 00:00:00
abstract：:In swine the upper reproductive tract undergoes early postnatal development, however little is known about the lower reproductive tract. Our objective was to measure cytology and proteome of vaginal swab samples taken on postnatal day (PND) 2 and 16 in gilts to determine if temporal changes occurred in cell and protei...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-49597-w
更新日期：2019-09-13 00:00:00
abstract：:Diabetic nephropathy is characterised by the excessive amount of extracellular matrix in glomeruli and tubulointerstitial space. Lysyl oxidase-like 2 (LOXL2) is elevated in renal fibrosis and known to play key roles in ECM stabilisation by facilitating collagen cross-links, epithelial to mesenchymal transition and myo...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-27462-6
更新日期：2018-06-21 00:00:00
abstract：:Artificial sheet materials, known as MetaSurfaces, have been applied to fully control both space and surface waves due to their exceptional abilities to dynamically tailor wave fronts and polarization states, while maintaining small footprints. However, previous and current designs and manufactured MetaSurfaces are li...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-36451-8
更新日期：2019-02-28 00:00:00
abstract：:Niemann-Pick type C1 (NPC1) disease is a rare autosomal recessive, neurodegenerative lysosomal storage disorder, which presents with a range of clinical phenotypes and hence diagnosis remains a challenge. In view of these difficulties, the search for a novel, NPC1-specific biomarker (or set of biomarkers) is a topic o...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-06264-2
更新日期：2017-07-24 00:00:00
abstract：:The opportunistic pathogen Staphylococcus aureus is responsible for causing infections related to indwelling medical devices, where this pathogen is able to attach and form biofilms. The intrinsic properties given by the self-produced extracellular biofilm matrix confer high resistance to antibiotics, triggering infec...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-75929-2
更新日期：2020-11-03 00:00:00
abstract：:The analysis of cell type-specific activity patterns during behaviors is important for better understanding of how neural circuits generate cognition, but has not been well explored from in vivo neurophysiological datasets. Here, we describe a computational approach to uncover distinct cell subpopulations from in vivo...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep12474
更新日期：2015-07-27 00:00:00
abstract：:Early fMRI studies suggested that brain areas processing self-related and other-related information were highly overlapping. Hypothesising functional localisation of the cortex, researchers have tried to locate "self-specific" and "other-specific" regions within these overlapping areas by subtracting suspected confoun...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep43313
更新日期：2017-03-03 00:00:00
abstract：:Adaptive optics (AO) visual simulators based on deformable mirrors, spatial light modulators or optotunable lenses are increasingly used to simulate vision through different multifocal lens designs. However, the correspondence of this simulation with that obtained through real intraocular lenses (IOLs) tested on the s...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-38673-w
更新日期：2019-02-07 00:00:00
abstract：:Stylosanthes guianensis (Aubl.) Sw. is an economically important pasture and forage legume in tropical regions of the world. Genetic improvement of the crop can be enhanced through marker-assisted breeding. However, neither single nucleotide polymorphism (SNP) markers nor SNP-based genetic linkage map has been previou...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-40489-7
更新日期：2019-03-07 00:00:00
abstract：:Theory predicts that social interactions can induce an alignment of behavioral asymmetries between individuals (i.e., population-level lateralization), but evidence for this effect is mixed. To understand how interaction with other individuals affects behavioral asymmetries, we systematically manipulated the social en...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-61410-7
更新日期：2020-03-11 00:00:00
abstract：:The artificial earthquake of mb 6.1 related to the North Korea's sixth nuclear test occured at Mt. Mantap, North Korea on September 3, 2017. It was reported that a large and complex surface deformation was caused by the event. The surface deformation was composed of expansion of explosions, collapse, compaction and la...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-74957-2
更新日期：2020-10-20 00:00:00
abstract：:Stress-induced sensitization represents a process whereby prior exposure to severe stressors leaves animals or humans in a hyper-responsive state to further stressors. Indeed, this phenomenon is assumed to be the basis of certain stress-associated pathologies, including post-traumatic stress disorder and psychosis. On...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep31244
更新日期：2016-08-11 00:00:00
abstract：:Myocardial ischemia/reperfusion (I/R) injury detrimentally alters the prognosis of patients undergoing revascularization after acute myocardial infarction. Our previous study demonstrated that NF-κB-induced autophagy plays a detrimental role in cardiac I/R injury using a rabbit myocardial I/R model. In this study, we ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep37448
更新日期：2016-11-18 00:00:00
abstract：:Functional neuroimaging studies have implicated regions of both ventrolateral prefrontal cortex (VLPFC) and angular gyrus in processes associated with retrieving goal-relevant information, which increases the fidelity and richness of long-term memory (LTM). To further investigate the roles of these cortical regions as...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-33164-w
更新日期：2018-10-05 00:00:00
abstract：:BST-2/tetherin is an interferon-inducible antiviral protein that blocks the release of various enveloped viruses, including HIV-1. Hepatitis B virus (HBV), a major cause of liver disease, belongs to the Hepadnaviridae family of enveloped DNA viruses. Whether BST-2 regulates HBV production is largely unknown. In this r...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep11736
更新日期：2015-06-29 00:00:00
abstract：:Adult hippocampal neurogenesis is associated with learning and affective behavioural regulation. Its diverse functionality is segregated along the septotemporal axis from the dorsal to ventral hippocampus. However, features distinguishing immature neurons in these regions have yet to be characterized. Additionally, al...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep30467
更新日期：2016-07-29 00:00:00
abstract：:There is a huge request for the development of low dielectric constant polymeric materials for microelectronic applications. In thisstudy, polymer blends based on PVA:POZ with low dielectric constant has been fabricated. The results of XRD indicate that crystalline domain is enhanced at higher POZ concentration. Brill...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-49715-8
更新日期：2019-09-11 00:00:00
abstract：:Iron is vital for nearly all living organisms, but during infection, not readily available to pathogens. Infectious bacteria therefore depend on specialized mechanisms to survive when iron is limited. These mechanisms make attractive targets for new drugs. Here, by genome-wide phenotypic profiling, we identify and cat...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-47905-y
更新日期：2019-08-06 00:00:00
abstract：:Nucleic acid polymers (NAPs) block the release of subviral particles from hepatocytes, a mechanism consistent with their antiviral activity against hepatitis B virus (HBV) in patients. Analysis of immunostimulatory properties of NAPs were conducted with several NAP species: REP 2006, the prototypic degenerate NAP [dN]...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep43838
更新日期：2017-03-08 00:00:00
abstract：:The mechanisms by which the airborne pathogen Mycobacterium tuberculosis spreads within the lung and leaves its primary niche to colonize other organs, thus inducing extrapulmonary forms of tuberculosis (TB) in humans, remains poorly understood. Herein, we used a transcriptomic approach to investigate the host cell ge...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep33162
更新日期：2016-09-12 00:00:00
abstract：:Bisacylphosphane oxides (BAPOs) are established as photoinitiators for industrial applications. Light irradiation leads to their photolysis, producing radicals. Radical species induce oxidative stress in cells and may cause cell death. Hence, BAPOs may be suitable as photolatent cytotoxic agents, but such applications...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-42026-y
更新日期：2019-04-12 00:00:00
abstract：:Post-glycosylphosphatidylinositol (GPI) attachment to proteins 3, also known as PGAP3 or PERLD1 (PER1-like domain-containing protein 1), participates in the lipid remodeling process of glycosylphosphatidylinositol (GPI) anchor proteins during post-translational modification. Functional defect in PERLD1 was previously ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-57592-9
更新日期：2020-01-20 00:00:00
abstract：:Atlantic bluefin tuna (Thunnus thynnus) from the two main spawning populations in the Mediterranean and Gulf of Mexico occur together in the western, central and eastern Atlantic. Stock composition of catches from mixing areas is uncertain, presenting a major challenge to the sustainable management of the fisheries. T...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-71355-6
更新日期：2020-09-07 00:00:00
abstract：:The phosphatases of regenerating liver (PRLs) are involved in tumorigenesis and metastatic cancer yet their cellular function remains unclear. Recent reports have shown PRL phosphatases bind tightly to the CNNM family of membrane proteins to regulate magnesium efflux. Here, we characterize the interactions between the...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-00147-2
更新日期：2017-03-03 00:00:00
abstract：:Immune responses against gene therapy products limit its therapeutic efficacy and present a safety risk. Identification of agents that blunt immune reactions may aid in developing novel immunomodulatory therapies. Fingolimod (FTY720) is an FDA approved immunomodulatory drug for treating multiple sclerosis that inhibit...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-29355-0
更新日期：2018-07-19 00:00:00
