Abstract:
:MicroRNAs (miRNAs) are single‑stranded RNA species that constitute a class of non‑coding RNAs, and are emerging as key regulators of gene expression. Since each miRNA is capable of regulating multiple genes, miRNAs are attractive markers for studies of coordinated gene expression. In this study, we investigated miRNA expression profiling using a massively parallel sequencing technique to compare non‑small‑cell lung cancer (NSCLC) tissue and normal lung tissue. Lung cancer tissue and normal lung tissue were obtained from nine NSCLC patients. RNA isolated from these samples was processed using RNA sequencing (RNA Seq) and the HiSeq 2000 system. Differentially expressed miRNAs and mRNAs were analyzed using a t‑test. We selected target pairs that showed a negative correlation among significantly differentially expressed miRNAs and their putative target mRNAs using miRBase Targets. The differences in the expression levels of 222 miRNAs and 1,597 genes were statistically significant, as indicated by an absolute fold change ≥1.5 and P<0.05. miR‑577, miR‑301b, miR‑944, miR‑891a and miR‑615‑3p were generally upregulated, and miR‑338‑3p was generally downregulated. miRNA‑mRNA target pair analysis revealed that 49 miRNAs had 696 target mRNAs. There were significantly differentially expressed miRNAs and mRNAs between lung cancer and normal tissue. Further investigation of miRNAs and their target genes is warranted to better understand NSCLC.
journal_name
Mol Med Repjournal_title
Molecular medicine reportsauthors
Lee HY,Han SS,Rhee H,Park JH,Lee JS,Oh YM,Choi SS,Shin SH,Kim WJdoi
10.3892/mmr.2014.2890subject
Has Abstractpub_date
2015-03-01 00:00:00pages
2034-40issue
3eissn
1791-2997issn
1791-3004journal_volume
11pub_type
杂志文章abstract::Human immunodeficiency virus‑1 (HIV‑1) infection severely damages the gut‑associated lymphoid tissue (GALT), the immune system and the gut barrier, which leads to accelerating the disease progression for patients with acquired immune deficiency syndrome (AIDS). Dysregulation of microRNAs (miRNAs) may contribute to thi...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2017.7722
更新日期:2017-12-01 00:00:00
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journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2015.3840
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journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2016.5875
更新日期:2016-12-01 00:00:00
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journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2016.5803
更新日期:2016-11-01 00:00:00
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journal_title:Molecular medicine reports
pub_type: 杂志文章,评审
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journal_title:Molecular medicine reports
pub_type: 杂志文章
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journal_title:Molecular medicine reports
pub_type: 杂志文章
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更新日期:2016-09-01 00:00:00
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journal_title:Molecular medicine reports
pub_type: 杂志文章
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更新日期:2018-09-01 00:00:00
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journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2014.2402
更新日期:2014-10-01 00:00:00
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journal_title:Molecular medicine reports
pub_type: 杂志文章
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更新日期:2019-08-01 00:00:00
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journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2017.7576
更新日期:2017-11-01 00:00:00
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journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2020.11625
更新日期:2020-12-01 00:00:00
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journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2013.1775
更新日期:2014-01-01 00:00:00
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journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2017.6877
更新日期:2017-09-01 00:00:00
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journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2020.11117
更新日期:2020-07-01 00:00:00
abstract::Neuregulin-1 (NRG-1) is a type of epidermal growth factor‑like protein primarily distributed in the nervous and cardiovascular systems. When sepsis occurs, the incidence of cardiac dysfunction in myocardial injury is high and the mechanism is complicated. It directly causes myocardial cell damage, whilst also causing ...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2020.11034
更新日期:2020-06-01 00:00:00
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journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2017.8039
更新日期:2018-01-01 00:00:00
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journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2015.4016
更新日期:2015-10-01 00:00:00
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journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2018.9447
更新日期:2018-11-01 00:00:00
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journal_title:Molecular medicine reports
pub_type: 临床试验,杂志文章
doi:10.3892/mmr.2012.783
更新日期:2012-05-01 00:00:00
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journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2014.2049
更新日期:2014-05-01 00:00:00
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pub_type: 杂志文章
doi:10.3892/mmr.2014.2545
更新日期:2014-11-01 00:00:00
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journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2020.11058
更新日期:2020-06-01 00:00:00
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journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2017.7809
更新日期:2017-12-01 00:00:00
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journal_title:Molecular medicine reports
pub_type: 杂志文章
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更新日期:2015-10-01 00:00:00
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journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2012.962
更新日期:2012-09-01 00:00:00
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journal_title:Molecular medicine reports
pub_type: 杂志文章
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更新日期:2015-05-01 00:00:00
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journal_title:Molecular medicine reports
pub_type: 杂志文章,meta分析
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更新日期:2013-04-01 00:00:00
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journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2013.1444
更新日期:2013-06-01 00:00:00
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journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2017.7597
更新日期:2017-12-01 00:00:00