Abstract:
:Voltage-gated sodium channels (Na(v)) underlie the rapid upstroke of action potentials in excitable tissues. Binding of channel-interactive proteins is essential for controlling fast and long-term inactivation. In the structure of the complex of the carboxy-terminal portion of Na(v)1.5 (CTNa(v)1.5) with calmodulin (CaM)-Mg(2+) reported here, both CaM lobes interact with the CTNa(v)1.5. On the basis of the differences between this structure and that of an inactivated complex, we propose that the structure reported here represents a non-inactivated state of the CTNa(v), that is, the state that is poised for activation. Electrophysiological characterization of mutants further supports the importance of the interactions identified in the structure. Isothermal titration calorimetry experiments show that CaM binds to CTNa(v)1.5 with high affinity. The results of this study provide unique insights into the physiological activation and the pathophysiology of Na(v) channels.
journal_name
Nat Communjournal_title
Nature communicationsauthors
Gabelli SB,Boto A,Kuhns VH,Bianchet MA,Farinelli F,Aripirala S,Yoder J,Jakoncic J,Tomaselli GF,Amzel LMdoi
10.1038/ncomms6126subject
Has Abstractpub_date
2014-11-05 00:00:00pages
5126issn
2041-1723pii
ncomms6126journal_volume
5pub_type
杂志文章abstract::Graphene is an ideal medium for long-distance spin communication in future spintronic technologies. So far, the prospect is limited by the smaller sizes of exfoliated graphene flakes and lower spin transport properties of large-area chemical vapour-deposited (CVD) graphene. Here we demonstrate a high spintronic perfor...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms7766
更新日期:2015-04-10 00:00:00
abstract::The transcriptional regulatory network (TRN) of Bacillus subtilis coordinates cellular functions of fundamental interest, including metabolism, biofilm formation, and sporulation. Here, we use unsupervised machine learning to modularize the transcriptome and quantitatively describe regulatory activity under diverse co...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-20153-9
更新日期:2020-12-11 00:00:00
abstract::In many dynamical systems and complex networks time delays appear naturally in feedback loops or coupling connections of individual elements. Moreover, in a whole class of systems, these delay times can depend on the state of the system. Nevertheless, so far the understanding of the impact of such state-dependent dela...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms8425
更新日期:2015-06-17 00:00:00
abstract::Silk and silk fibroin, the biomaterial from nature, nowadays are being widely utilized in many cutting-edge micro/nanodevices/systems via advanced micro/nanofabrication techniques. Herein, for the first time to our knowledge, we report aqueous multiphoton lithography of diversiform-regenerated-silk-fibroin-centric ink...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms9612
更新日期:2015-10-16 00:00:00
abstract::Stable benzotriazinyl radicals (Blatter's radicals) recently attracted considerable interest as building blocks for functional materials. The existing strategies to derivatize Blatter's radicals are limited, however, and synthetic routes are complex. Here, we report that an inexpensive, commercially available, analyti...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms15088
更新日期:2017-05-15 00:00:00
abstract::Bacterial contamination of biological channels, catheters or water resources is a major threat to public health, which can be amplified by the ability of bacteria to swim upstream. The mechanisms of this 'rheotaxis', the reorientation with respect to flow gradients, are still poorly understood. Here, we follow individ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-11360-0
更新日期:2019-07-31 00:00:00
abstract::Intricate biomineralization processes in molluscs engineer hierarchical structures with meso-, nano- and atomic architectures that give the final composite material exceptional mechanical strength and optical iridescence on the macroscale. This multiscale biological assembly inspires new synthetic routes to complex ma...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms10097
更新日期:2015-12-03 00:00:00
abstract::Owing to the significant difference in the numbers of simulated and experimentally feasible zeolite structures, several alternative strategies have been developed for zeolite synthesis. Despite their rationality and originality, most of these techniques are based on trial-and-error, which makes it difficult to predict...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-12882-3
更新日期:2019-11-12 00:00:00
abstract::Elevated glucose consumption is fundamental to cancer, but selectively targeting this pathway is challenging. We develop a high-throughput assay for measuring glucose consumption and use it to screen non-small-cell lung cancer cell lines against bioactive small molecules. We identify Milciclib that blocks glucose cons...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-13334-8
更新日期:2019-11-29 00:00:00
abstract::The Amazon river basin receives ~2000 mm of precipitation annually and contributes ~17% of global river freshwater input to the oceans; its hydroclimatic variations can exert profound impacts on the marine ecosystem in the Amazon plume region (APR) and have potential far-reaching influences on hydroclimate over the tr...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-18187-0
更新日期:2020-09-01 00:00:00
abstract::The original version of this Article contained an error in the spelling of the author Jianyun Yan, which was incorrectly given as Jiangyun Yan. This has now been corrected in both the PDF and HTML versions of the Article. ...
journal_title:Nature communications
pub_type: 杂志文章,已发布勘误
doi:10.1038/s41467-017-02617-7
更新日期:2018-01-12 00:00:00
abstract::Telomerase elongates the telomeric G-strand to prevent telomere shortening through conventional DNA replication. However, synthesis of the complementary C-strand by DNA polymerase α is also required to maintain telomere length. Polymerase α cannot perform this role without the ssDNA binding complex CST (CTC1-STN1-TEN1...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-05154-z
更新日期:2018-07-19 00:00:00
abstract::Scintillation based X-ray detection has received great attention for its application in a wide range of areas from security to healthcare. Here, we report highly efficient X-ray scintillators with state-of-the-art performance based on an organic metal halide, ethylenebis-triphenylphosphonium manganese (II) bromide ((C...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-18119-y
更新日期:2020-08-28 00:00:00
abstract::Naturally occurring single crystals having a multidomain morphology are a counterintuitive phenonomon: the macroscopic appearance is expected to follow the symmetry of the unit cell. Growing such crystals in the lab is a great challenge, especially from organic molecules. We achieve here uniform metallo-organic crysta...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-13925-5
更新日期:2020-01-20 00:00:00
abstract::Magnetic reconnection is a fundamental process in magnetized plasma where magnetic energy is converted to plasma energy. Despite huge differences in the physical size of the reconnection layer, remarkably similar characteristics are observed in both laboratory and magnetosphere plasmas. Here we present the comparative...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-07680-2
更新日期:2018-12-06 00:00:00
abstract::Intratumor heterogeneity (ITH) and tumor evolution have been well described for clear cell renal cell carcinomas (ccRCC), but they are less studied for other kidney cancer subtypes. Here we investigate ITH and clonal evolution of papillary renal cell carcinoma (pRCC) and rarer kidney cancer subtypes, integrating whole...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-16546-5
更新日期:2020-06-18 00:00:00
abstract::Advanced colorectal cancer is one of the deadliest cancers, with a 5-year survival rate of only 12% for patients with the metastatic disease. Checkpoint inhibitors, such as the antibodies inhibiting the PD-1/PD-L1 axis, are among the most promising immunotherapies for patients with advanced colon cancer, but their dur...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms12499
更新日期:2016-08-17 00:00:00
abstract::Studying emerging or neglected pathogens is often challenging due to insufficient information and absence of genetic tools. Dual RNA-seq provides insights into host-pathogen interactions, and is particularly informative for intracellular organisms. Here we apply dual RNA-seq to Orientia tsutsugamushi (Ot), an obligate...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-17094-8
更新日期:2020-07-03 00:00:00
abstract::One of the important challenges in condensed matter science is to understand ultrafast, atomic-scale fluctuations that dictate dynamic processes in equilibrium and non-equilibrium materials. Here, we report an important step towards reaching that goal by using a state-of-the-art perfect crystal based split-and-delay s...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-04178-9
更新日期:2018-04-27 00:00:00
abstract::Duchenne muscular dystrophy (DMD) is characterized by muscle degeneration and progressive weakness. There is considerable inter-patient variability in disease onset and progression, which can confound the results of clinical trials. Here we show that a common null polymorphism (R577X) in ACTN3 results in significantly...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms14143
更新日期:2017-01-31 00:00:00
abstract::Although chemically very simple, Fe2O3 is known to undergo a series of enigmatic structural, electronic and magnetic transformations at high pressures and high temperatures. So far, these transformations have neither been correctly described nor understood because of the lack of structural data. Here we report a syste...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms10661
更新日期:2016-02-11 00:00:00
abstract::Generally, a homogeneous catalyst exhibits good activity and defined active sites but it is difficult to recycle. Meanwhile, a heterogeneous catalyst can easily be reused but its active site is difficult to reveal. It is interesting to bridge the gap between homogeneous and heterogeneous catalysis via controllable con...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-03834-4
更新日期:2018-04-13 00:00:00
abstract::Replication-Factor-C (RFC) and RFC-like complexes (RLCs) mediate chromatin engagement of the proliferating cell nuclear antigen (PCNA). It remains controversial how RFC and RLCs cooperate to regulate PCNA loading and unloading. Here, we show the distinct PCNA loading or unloading activity of each clamp loader. ATAD5-R...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-10376-w
更新日期:2019-06-03 00:00:00
abstract::Histone deacetylase inhibitors (HDACi) are approved for treating certain haematological malignancies, however, recent evidence also illustrates they are modulators of the immune system. In experimental models, HDACi are particularly potent against malignancies originating from the B-lymphocyte lineage. Here we examine...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms7838
更新日期:2015-04-27 00:00:00
abstract::Natural killer (NK) cells are reported to have immunological memory, with CD49a+ liver-resident NK cells shown to confer hapten-specific memory responses, but how this memory is induced or maintained is unclear. Here we show that memory type I innate lymphoid cells (ILC1s), which express IL-7Rα, are generated in the l...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-07405-5
更新日期:2018-11-19 00:00:00
abstract::Proteins are versatile natural building blocks with highly complex and multifunctional architectures, and self-assembled protein structures have been created by the introduction of covalent, noncovalent, or metal-coordination bonding. Here, we report the robust, selective, and reversible metal coordination properties ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-13491-w
更新日期:2019-12-05 00:00:00
abstract::Metastasis is the primary cause for mortality in breast cancer. MicroRNAs, gene expression master regulators, constitute an attractive candidate to control metastasis. Here we show that breast cancer metastasis can be prevented by miR-96 or miR-182 treatment, and decipher the mechanism of action. We found that miR-96/...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms12868
更新日期:2016-09-19 00:00:00
abstract::The integration of β-barrel proteins into the bacterial outer membrane (OM) is catalysed by the β-barrel assembly machinery (BAM). The central BAM subunit (BamA) itself contains a β-barrel domain that is essential for OM protein biogenesis, but its mechanism of action is unknown. To elucidate its function, here we dev...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-11230-9
更新日期:2019-07-26 00:00:00
abstract::The antiquity and global abundance of the enzyme, RuBisCO, attests to the crucial and longstanding role it has played in the biogeochemical cycles of Earth over billions of years. The counterproductive oxygenase activity of RuBisCO has persisted over billions of years of evolution, despite its competition with the car...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms10382
更新日期:2016-01-21 00:00:00
abstract::Rare genetic disorders (RGDs) often exhibit significant clinical variability among affected individuals, a disease characteristic termed variable expressivity. Recently, the aggregate effect of common variation, quantified as polygenic scores (PGSs), has emerged as an effective tool for predictions of disease risk and...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-12869-0
更新日期:2019-10-25 00:00:00