Characterization of beta-adrenergic receptors in cultured human trabecular cells and in human trabecular meshwork.

Abstract:

:The characterization of beta-adrenergic receptors on cultured human trabecular cells and trabecular meshwork from human autopsy eyes was carried out by radioligand binding utilizing (125I)-iodopindolol. In cultured cells, the observed binding of (125I)-iodopindolol was of high affinity (Kd = 43 pM) and saturable. Scatchard plots were linear and revealed a Bmax of 33 +/- 7 fmol/mg of protein. Competition studies with a series of agonists and antagonists revealed that human trabecular cells contain a single class of beta-adrenergic receptors of the beta 2 subtype. Similarly, the IC50 of ICI 89,406 (176 nM) in human trabecular meshwork from autopsy eyes supports the presence of beta 2-adrenergic receptors in this tissue.

authors

Wax MB,Molinoff PB,Alvarado J,Polansky J

subject

Has Abstract

pub_date

1989-01-01 00:00:00

pages

51-7

issue

1

eissn

0146-0404

issn

1552-5783

journal_volume

30

pub_type

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