Abstract:
:Viruses have coevolved with their hosts, acquiring strategies to subvert host cellular pathways for effective viral replication and spread. Human cytomegalovirus (HCMV), a widely-spread β-herpesvirus, is a major cause of birth defects and opportunistic infections in HIV-1/AIDS patients. HCMV displays an intricate system-wide modulation of the human cell proteome. An impressive array of virus-host protein interactions occurs throughout the infection. To investigate the virus life cycle, proteomics has recently become a significant component of virology studies. Here, we review the mass spectrometry-based proteomics approaches used in HCMV studies, as well as their contribution to understanding the HCMV life cycle and the virus-induced changes to host cells. The importance of the biological insights gained from these studies clearly demonstrate the impact that proteomics has had and can continue to have on understanding HCMV biology and identifying new therapeutic targets.
journal_name
Expert Rev Proteomicsjournal_title
Expert review of proteomicsauthors
Jean Beltran PM,Cristea IMdoi
10.1586/14789450.2014.971116subject
Has Abstractpub_date
2014-12-01 00:00:00pages
697-711issue
6eissn
1478-9450issn
1744-8387journal_volume
11pub_type
杂志文章abstract:INTRODUCTION:Cutaneous leishmaniasis (CL), caused by different Leishmania parasite species, is associated with parasite-induced immune-mediated skin inflammation and ulceration. Whereas many CL studies focus on gene expression signatures in mouse models, the transcriptional response driving human patients in the field ...
journal_title:Expert review of proteomics
pub_type: 杂志文章
doi:10.1080/14789450.2020.1812390
更新日期:2020-01-01 00:00:00
abstract::Although genomics techniques such as DNA microarrays have been widely used in virology, much more limited use has been made of proteomics. Although difficult, proteomics can greatly contribute to an understanding of virus-cell interactions, including the ternary structure of viral receptors at the cell surface, post-t...
journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/14789450.2.4.577
更新日期:2005-08-01 00:00:00
abstract::Stroke is one of the most common causes of death worldwide and a major cause of acquired disability in adults. Despite advances in research during the last decade, prevention and treatment strategies still suffer from significant limitations, and therefore new theoretical and technical approaches are required. Technol...
journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/epr.12.33
更新日期:2012-08-01 00:00:00
abstract::This review addresses the challenges of neuroproteomics and recent progress in biomarkers and tests for neurodegenerative diseases, including Alzheimer's disease, Parkinson's disease and amyotrophic lateral sclerosis. The review will discuss how the application of quantitative 2D gel electrophoresis, combined with app...
journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/14789450.3.1.45
更新日期:2006-02-01 00:00:00
abstract::Helicobacter pylori infection is linked to the development of gastric cancer. Atrophic body gastritis is considered the first important step in the histogenesis of such neoplasia. H. pylori infection is involved in the induction of atrophic body gastritis, but documentation of H. pylori infection is difficult because ...
journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/epr.10.5
更新日期:2010-04-01 00:00:00
abstract::A major goal of proteomics is to develop methods that enable the systematic characterization of every protein within the cell or particular subcellular proteome using a single analytical platform. Although the equivalent has already been achieved in genomics, reaching this goal in proteomics represents a much greater ...
journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/14789450.1.2.153
更新日期:2004-08-01 00:00:00
abstract:INTRODUCTION:The skin protects the human body from external insults and regulates water and temperature homeostasis. A highly developed extracellular matrix (ECM) supports the skin and instructs its cell functions. Reduced functionality of the ECM is often associated with skin diseases that cause physical impairment an...
journal_title:Expert review of proteomics
pub_type: 杂志文章
doi:10.1080/14789450.2020.1773261
更新日期:2020-05-01 00:00:00
abstract:INTRODUCTION:Skeletal muscle is the major site of insulin-stimulated glucose uptake and imparts the beneficial effects of exercise, and hence is an important site of insulin resistance in obesity and type 2 diabetes (T2D). Despite extensive molecular biology-oriented research the molecular mechanisms underlying insulin...
journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1080/14789450.2018.1528147
更新日期:2018-10-01 00:00:00
abstract:INTRODUCTION:In several biomedical research fields, the cross-linking of peptides and proteins has an important impact on health and wellbeing. It is therefore of crucial importance to study this class of post-translational modifications in detail. The huge potential of mass spectrometric technologies in the mapping of...
journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1080/14789450.2017.1362336
更新日期:2017-10-01 00:00:00
abstract::Proteins have several measurable features in biological fluids that may change under pathological conditions. The current disease biomarker discovery is mostly based on protein concentration in the sample as the measurable feature. Changes in protein structures, such as post-translational modifications and in protein-...
journal_title:Expert review of proteomics
pub_type: 杂志文章
doi:10.1586/14789450.2016.1116945
更新日期:2016-01-01 00:00:00
abstract::Introduction: Glycomics, which aims to define the glycome of a biological system to better assess the biological attributes of the glycans, has attracted increasing interest. However, the complexity and diversity of glycans present challenging barriers to glycome definition. Technological advances are major drivers in...
journal_title:Expert review of proteomics
pub_type: 杂志文章
doi:10.1080/14789450.2020.1708199
更新日期:2020-01-01 00:00:00
abstract::In recent years, MALDI imaging mass spectrometry (MALDI-IMS) has developed as a promising tool to investigate the spatial distribution of biomolecules in intact tissue specimens. Ion densities of various molecules can be displayed as heat maps while preserving anatomical structures. In this short review, an overview o...
journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/14789450.2013.814939
更新日期:2013-08-01 00:00:00
abstract::Metastasis is the main cause of cancer death. As the tumor progresses, cells from the primary tumor site are shed into the bloodstream as circulating tumor cells (CTCs). Eventually, these cells colonize other organs and form distant metastases. It is therefore imperative that we gain a better understanding of the biol...
journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/14789450.2013.858021
更新日期:2013-12-01 00:00:00
abstract::Protein phosphorylation events are key regulators of cellular signaling processes. In the era of functional genomics, rational drug design programs demand large-scale high-throughput analysis of signal transduction cascades. Significant improvements in the area of mass spectrometry-based proteomics have provided excit...
journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/14789450.1.3.343
更新日期:2004-10-01 00:00:00
abstract::Spermatozoa are unique cells that have highly compact DNA, motility (and hypermotility) patterns, a specific morphology, localized mitochondria and an apical acrosome. They are the end product of a dynamic process termed spermatogenesis. Sperm are therefore produced with specific proteins in order to effect different ...
journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/14789450.2016.1151357
更新日期:2016-01-01 00:00:00
abstract:INTRODUCTION:The lack of biomarkers indicating involved nociceptive and/or pain mechanisms makes diagnostic procedures problematic. Clinical pain research has begun to use proteomics. AREAS COVERED:This systematic review covers proteomic studies of chronic pain cohorts and in relation to clinical variables. Searches i...
journal_title:Expert review of proteomics
pub_type: 杂志文章
doi:10.1080/14789450.2020.1797499
更新日期:2020-06-01 00:00:00
abstract:INTRODUCTION:Cell-free protein microarrays represent a special form of protein microarray which display proteins made fresh at the time of the experiment, avoiding storage and denaturation. They have been used increasingly in basic and translational research over the past decade to study protein-protein interactions, t...
journal_title:Expert review of proteomics
pub_type: 杂志文章
doi:10.1080/14789450.2018.1415146
更新日期:2018-01-01 00:00:00
abstract::Selected reaction monitoring (SRM) is a highly selective and sensitive mass spectrometric methodology for precise and accurate quantification of low-abundant proteins in complex mixtures and for characterization of modified peptides, and constitutes the method of choice in targeted proteomics. Owing to its outstanding...
journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/epr.11.11
更新日期:2011-04-01 00:00:00
abstract::High-throughput proteomics technologies tend to provide highly sensitive information about living tissues and biological fluids. Analytes are characterized by intrinsic and extrinsic properties, the latter depending on each phase of their preparation, sometimes adding artifacts with crucial repercussions in result rel...
journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/14789450.4.3.363
更新日期:2007-06-01 00:00:00
abstract::Tissue microarrays are a high-throughput method for the investigation of biomarkers in multiple tissue specimens at once. This technique allows for the analysis of up to 500 tissue samples in a single experiment using immunohistochemistry and in situ hybridization. Recently, cell lines and xenografts have been reduced...
journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/14789450.2.3.325
更新日期:2005-06-01 00:00:00
abstract::Huntington's disease (HD) is caused by expansion of a polyglutamine repeat in the N-terminal region of huntingtin (htt), a large protein that has been found to interact with a variety of proteins. It remains to be determined how the interactions of htt with other proteins are involved in the pathogenesis of HD. A rece...
journal_title:Expert review of proteomics
pub_type: 评论,杂志文章
doi:10.1586/epr.11.72
更新日期:2012-01-01 00:00:00
abstract::An essential skill for every researcher is to learn how to select and apply the most appropriate methods for the questions they are trying to answer. With the extensive variety of methods available, it is increasingly important to scrutinize the advantages and disadvantages of these techniques prior to making a decisi...
journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/epr.10.10
更新日期:2010-06-01 00:00:00
abstract::A panel of biomarkers for the early detection of bladder cancer has not yet been identified. Many different molecules, including DNA, RNA or proteins have been reported but none have provided adequate sensitivity for a single-tier screening test or a test to replace cystoscopy. Therefore, multimarker panels are discus...
journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/epr.10.82
更新日期:2010-12-01 00:00:00
abstract::Antigen processing forwards various information about the cellular status and the proteome to the cell surface for scrutiny by the cellular immune system. Thus the repertoire of major histocompatibility complex (MHC)-bound peptides and the MHC ligandome, indirectly mirrors the proteome in order to make alterations ins...
journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/14789450.3.6.653
更新日期:2006-12-01 00:00:00
abstract::Mammalian sperm are differentiated germ cells that transfer genetic material from the male to the female. Owing to this essential role in the reproductive process, an understanding of the complex mechanisms that underlie sperm function has implications ranging from the development of novel contraceptives to the treatm...
journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/epr.12.41
更新日期:2012-10-01 00:00:00
abstract::Ovarian cancer is the fifth leading cause of cancer deaths among North American women. Regrettably, there is currently no reliable circulating biomarker that can detect ovarian cancer in its early stages. The CA125 biomarker is very useful for treatment response monitoring, but its sensitivity is very low for early de...
journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/14789450.4.1.121
更新日期:2007-02-01 00:00:00
abstract::Introduction: Degradation of proteins by cellular proteasomes is critical for the fidelity of protein homeostasis and proper cell function. Indeed, perturbations in proteasome function, as well as the degradation of specific substrates, are associated with a variety of human diseases. Yet, monitoring and analyzing pro...
journal_title:Expert review of proteomics
pub_type: 杂志文章
doi:10.1080/14789450.2019.1645601
更新日期:2019-07-19 00:00:00
abstract::Clinical proteomics has been applied to the identification of biomarkers of obstetric and neonatal disease. We will discuss a number of encouraging studies that have led to potentially valid biomarkers in the context of Down's syndrome, preterm birth, amniotic infections, preeclampsia, intrauterine growth restriction ...
journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/14789450.2014.872564
更新日期:2014-02-01 00:00:00
abstract::Prenatal diagnosis is now offered to high-risk pregnancies, including advanced maternal age, ultrasound anomalies and positive Down's syndrome screening, and karyotype on cultured fetal material is the test of choice to screen these pregnancies. However, microscope analysis can only detect gross chromosome abnormaliti...
journal_title:Expert review of proteomics
pub_type: 评论,杂志文章
doi:10.1586/epr.13.9
更新日期:2013-04-01 00:00:00
abstract::This article reviews the current state of systems biology approaches, including the experimental tools used to generate 'omic' data and computational frameworks to interpret this data. Through illustrative examples, systems biology approaches to understand gene expression and gene expression regulation are discussed. ...
journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/14789450.2.6.915
更新日期:2005-12-01 00:00:00