Abstract：
:In this paper, we present a strategy to improve delivery of femtosecond laser pulses from a regenerative amplifier through a hollow core photonic crystal fiber for temporally focused wide-field two-photon endomicroscopy. For endomicroscope application, wide-field two-photon excitation has the advantage of requiring no scanning in the distal end. However, wide-field two-photon excitation requires peak power that is 10(4)-10(5) times higher than the point scanning approach corresponding to femtosecond pulses with energy on the order of 1-10 μJ at the specimen plane. The transmission of these high energy pulses through a single mode fiber into the microendoscope is a significant challenge. Two approaches were pursued to partially overcome this limitation. First, a single high energy pulse is split into a train of pulses with energy below the fiber damage threshold better utilizing the available laser energy. Second, stretching the pulse width in time by introducing negative dispersion was shown to have the dual benefit of reducing fiber damage probability and compensating for the positive group velocity dispersion induced by the fiber. With these strategy applied, 11 fold increase in the two photon excitation signal has been demonstrated.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Choi H,So PTdoi
10.1038/srep06626subject
Has Abstractpub_date
2014-10-15 00:00:00pages
6626issn
2045-2322pii
srep06626journal_volume
4pub_type
杂志文章abstract：:Cellvibrio mixtus strain J3-8 is a gram-negative, xylanase-producing aerobic soil bacterium isolated from giant snails in Singapore. It is able to produce up to 10.1 U ml(-1) of xylanase, which is comparable to xylanase production from known bacterial and fungal strains. Genome sequence analysis of strain J3-8 reveals...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep10521
更新日期：2015-05-21 00:00:00
abstract：:A new miniplate applied differently from conventional application method for bone fixation has been developed. The novel approach is the insertion of the screw into the bone before miniplate installation. This study aimed to assess the stress distribution of a newly designed Yang's Keyhole (YK)- plate for segmental-bo...
journal_title：Scientific reports
pub_type： 杂志文章
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abstract：:ADC is a potential post treatment imaging biomarker in colorectal liver metastasis however measurements are affected by respiratory motion. This is compounded by increased statistical uncertainty in ADC measurement with decreasing tumour volume. In this prospective study we applied a retrospective motion correction me...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-40565-y
更新日期：2019-03-07 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-15372-y
更新日期：2017-11-07 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-02074-8
更新日期：2017-05-15 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2020-03-19 00:00:00
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pub_type： 杂志文章
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pub_type： 杂志文章
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更新日期：2018-08-03 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2017-11-07 00:00:00
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pub_type： 杂志文章
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更新日期：2020-05-08 00:00:00
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pub_type： 杂志文章
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更新日期：2019-12-20 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2017-01-27 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2018-09-18 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-48269-z
更新日期：2019-08-12 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep21269
更新日期：2016-02-18 00:00:00
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journal_title：Scientific reports
pub_type： 已发布勘误
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更新日期：2020-02-18 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-38546-2
更新日期：2019-02-14 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-39182-6
更新日期：2019-02-27 00:00:00
abstract：:The atomic-scale response of dielectrics/ferroelectrics to electric fields is central to their functionality. Here we introduce an in situ characterization method that reveals changes in the local atomic structure in polycrystalline materials under fields. The method employs atomic pair distribution functions (PDFs), ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep14678
更新日期：2015-10-01 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-29713-y
更新日期：2018-08-15 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep06392
更新日期：2014-09-22 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2020-08-19 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2017-03-07 00:00:00
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journal_title：Scientific reports
pub_type： 临床试验,杂志文章
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更新日期：2019-06-19 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep10582
更新日期：2015-06-08 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-67110-6
更新日期：2020-06-26 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-04589-6
更新日期：2017-06-30 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-46602-0
更新日期：2019-07-15 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-44787-y
更新日期：2019-06-06 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep20453
更新日期：2016-02-09 00:00:00
