Abstract:
:The response to wounds until healing requires the activity of many cell types coordinate in space and time, so that the types of cells in a wound and their localization may be of help to date lesions with respect to death, which would be useful in forensic pathology. Cells reacting to injury include dendritic cells; the early reaction of these cells to skin wounding has not yet been investigated in humans, which was the aim of this study. Samples of wounded and control skin were taken at autopsy and analyzed by affinity histochemistry. Both epidermal and dermal MHC-II+ cells increased transiently in number within the first hour after wounding, then decreased. In the epidermis the increase affected also CD1a+ cells, i.e. well differentiated Langherhans cells, which however increased less, earlier and for a shorter time period than MHC-II+ cells. Dermal MHC-II+ cells became part of a perivascular mononuclear cell infiltrate visible in the subpapillary dermis by 60 min after wounding, which contained also mast cells. The immediately perivascular MHC-II+ cells were DC-SIGN- and CD11c-, while MHC-II+, DC-SIGN+, CD11c+ dendritic cells were predominantly located at the periphery of infiltrates and some were near the epidermis. Mast cells underwent degranulation, besides increase in number, in the first hours after wounding. The results suggest that skin dendritic cells, including Langerhans cells, participate to the early response to wounding in concert with mast cells, and that subpapillary blood vessels are primary sites of cell infiltration during that response in humans. The results show that the ratio between CD1a positive and MHC-II positive cells in the epidermis, the degranulation index of mast cells and the relative volume of MHC-II positive cells in the dermis can be added to the tools useful to distinguish vital from post mortem lesions and, the first two of them, to estimate the interval between a lesion and death.
journal_name
Forensic Sci Intjournal_title
Forensic science internationalauthors
Bacci S,Defraia B,Cinci L,Calosi L,Guasti D,Pieri L,Lotti V,Bonelli A,Romagnoli Pdoi
10.1016/j.forsciint.2014.08.024subject
Has Abstractpub_date
2014-11-01 00:00:00pages
179-85eissn
0379-0738issn
1872-6283pii
S0379-0738(14)00350-8journal_volume
244pub_type
杂志文章abstract::With research providing conflicting results from different habitats across the globe, the effect of clothing on decomposition is unclear; some studies indicate clothing increases decomposition rate by facilitating increased insect activity, and others conclude clothing prolongs the decay process. In South Africa, such...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2020.110197
更新日期:2020-04-01 00:00:00
abstract::The DNA identification of decomposed human remains for coronial investigations at the Victorian Institute of Forensic Medicine routinely requires the retrieval and processing of a bone sample obtained from the deceased. Bone is a difficult sample type to work with as it requires surgical removal from the deceased, ref...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2015.10.025
更新日期:2016-01-01 00:00:00
abstract::DNA molecular techniques were used in a forensic investigation involving the poaching of wildlife in a national park of Italy. A poacher, after having snared a wild boar (Sus scrofa) sow, knifed it to death. The animal was retrieved by conservation officers at the scene before the poacher could remove the carcass. Sub...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2005.04.032
更新日期:2005-10-29 00:00:00
abstract::The bodies of socially isolated people may remain undiscovered in their own houses for prolonged periods. Occasionally the body is in situ for sufficient time to become skeletonised, or partially so. Medico-legal investigation of these cases is complicated by degradation and contamination of evidence. Thus, a multidis...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2005.02.016
更新日期:2005-07-16 00:00:00
abstract::As part of a transcultural investigation of violent behaviour in Argentina and Denmark an analysis was made of the incidents involving deliberate violence as registered in the emergency wards of Hospital Fernandez and Hospital Pinero in Capital Federal Buenos Aires over a period of 13 and 17 months, respectively. In t...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/0379-0738(89)90208-9
更新日期:1989-07-01 00:00:00
abstract::The authors describe a technique for the gross examination of postmortem eyes of children who are suspected to have been the victims of deliberate trauma. By removing the anterior segment (cornea, iris, lens and pars plicata of the ciliary body) en bloc by a coronal incision through the pars plana just anterior to the...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/0379-0738(92)90149-q
更新日期:1992-09-01 00:00:00
abstract::Human hair is among the most common kind of evidence secured at crime scenes. Although DNA analysis through STR-typing is possible in principle, it is not very promising for telogenic hair or single hairs. For the mixed traces frequently found in practice, composed of different hair from an unknown number of individua...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2008.12.018
更新日期:2009-03-10 00:00:00
abstract::Human teeth are biological structures that resist extreme conditions thus becoming a useful source of DNA for human forensic identification purposes. When it is possible, forensic prefer only non-damaged teeth whereas those with cavities are usually rejected to avoid both external and internal bacterial contamination....
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2015.08.021
更新日期:2015-12-01 00:00:00
abstract::Forensic odontologists are involved in all phases of disaster victim identification (DVI). The failure of DVI management to embed odontology teams within all phases of the investigation and to include them in management decisions throughout the operation may lead to delays in the reconciliation process and could possi...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2010.08.013
更新日期:2011-02-25 00:00:00
abstract::The persistence of fibres under outdoor conditions is seldom investigated. This research aimed to study simultaneously the influence of several factors (and their cross-interactions) on the persistence of fibres on static textile surfaces. In order to achieve this aim, a design of experiments was successfully implemen...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2020.110593
更新日期:2021-01-01 00:00:00
abstract::Synthetic cannabinoids are a group of new psychoactive compounds (NPS) that act as agonists at the cannabinoid receptor. First reported in 2008, they currently represent one of the largest groups of NPS that are monitored by the European Monitoring Centre for Drugs and Drug Addiction (EMCDDA). Five samples (4 from the...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2017.10.020
更新日期:2017-12-01 00:00:00
abstract::Foreign objects as acquired characteristics in footwear examination have a great value in declaring a proposed identification. However, they do not have the same significance in excluding any footwear because they may disappear for many reasons as time passes by. The aim of this study was to establish the value of for...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2019.109949
更新日期:2019-11-01 00:00:00
abstract::Torture appears to be a permanent feature in countries, which have experienced military coups or ruled by oppressive governments in the past, such as Turkey. The Human Rights Foundation of Turkey (HRFT) was established in 1990 to serve torture victims, mainly those who were the victims of the 1980 military regime. Sin...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2012.12.019
更新日期:2013-03-10 00:00:00
abstract::We describe a method for obtaining specific and reproducible deoxyribonuclease I (DNase I) typing from liquid semen. Isoelectric focusing of the enzymes on polyacrylamide gel (IEF-PAGE, pH 3.5-5) was accomplished using a 0.5-mm thick gel. The separated isozymes were visualized by a new activity staining method, dried ...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/0379-0738(92)90043-v
更新日期:1992-11-01 00:00:00
abstract:BACKGROUND:Multiple genome-wide association studies (GWAS) and targeted gene sequencing have identified common variants in SCN10A in cases of PR and QRS duration abnormalities, atrial fibrillation and Brugada syndrome. The New York City Office of Chief Medical Examiner has now also identified five SCN10A variants of un...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2019.05.042
更新日期:2019-08-01 00:00:00
abstract::A large-scale study of facial soft tissue depths of Caucasian adults was conducted. Over a 2-years period, 967 Caucasian subjects of both sexes, varying age and varying body mass index (BMI) were studied. A user-friendly and mobile ultrasound-based system was used to measure, in about 20min per subject, the soft tissu...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2006.02.034
更新日期:2006-05-15 00:00:00
abstract::Cigarettes are consumer products with a broad market-driven orientation. In order to satisfy the different needs of smokers, cigarette trademarks with different aroma, taste and appearance are available on the market. In this study near infrared (NIR) spectroscopy in combination with chemometrics was applied in order ...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2018.10.016
更新日期:2019-01-01 00:00:00
abstract::The amplification of the STR DYS391, using the primers described in the Genome Data Base (GDB: G00-365-251), shows not only an additional band to the Y-specific one in males with a size range of 26 bp less than those of DYS391 locus alleles, but also a polymorphic pattern in females in the same size range as the addit...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/s0379-0738(00)00171-7
更新日期:2000-07-24 00:00:00
abstract::Molecular analyses in a post-mortem setting are becoming increasingly common, particularly in cases of sudden unexplained death, with the aim of identifying genetic mutations which may be responsible for causing death. In retrospective investigations, the access to suitable autopsy biological samples may be limited, a...
journal_title:Forensic science international
pub_type: 杂志文章,评审
doi:10.1016/j.forsciint.2017.09.020
更新日期:2017-11-01 00:00:00
abstract::There is considerable value in developing tools capable of accurately and reliably determining when bruises were inflicted in humans. Previous work has focused on the visual changes observed in a bruise as the injury develops and heals. However, due to variables such as how and where on the body the bruise was inflict...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2013.04.036
更新日期:2013-09-10 00:00:00
abstract::In autopsy files from April 1999 to April 2001, five cases showed macroscopic subendocardial small infarct above 1 cm diameter in the superior ventricular septum (SVS) near the atrioventricular (AV) junction, and all five were finally considered to be sudden cardiac death after full investigation. All these small infa...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2003.08.006
更新日期:2003-12-17 00:00:00
abstract::Forensic personnel may face a daunting task when searching for buried weapons at crime scenes or potential disposal sites. In particular, it is common to search for a small firearm that was discarded or buried by a perpetrator. When performing forensic searches, it is recommended to first use non-invasive methods such...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2010.10.019
更新日期:2011-05-20 00:00:00
abstract:BACKGROUND:The mandibular bone is an important part of the forensic facial reconstruction and it has the possibility of getting lost in skeletonized remains; for this reason, it is necessary to facilitate the identification process simulating the mandibular position only through craniomaxillary measures, for this task,...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2015.12.025
更新日期:2016-04-01 00:00:00
abstract::Neonatal hair is a clinically important toxicological matrix, as it allows determination of in utero drug exposure. This paper serves to review the physiological development of the hair follicle and hair production during fetal life. An understanding of the mechanisms and timing of hair development in the prenatal per...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2009.12.024
更新日期:2010-03-20 00:00:00
abstract::Facial soft tissue thickness measurement can be useful among several medico-legal techniques that aim to establish the identity of skeletal remains. This study examined the soft tissue thickness that covered the faces of autopsied cadavers sent to the Medico-Legal Institute of Guarulhos from September 2010 to Septembe...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2013.05.024
更新日期:2013-09-10 00:00:00
abstract::The main challenge for the identification and differentiation of lipsticks for forensic purposes is the fact that they have a similar chemical composition - in particular, samples of the same hue. The objective of the presented research was to solve this problem using a nondestructive analytical technique - attenuated...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2017.09.019
更新日期:2017-11-01 00:00:00
abstract::A sensitive and specific liquid chromatography tandem mass spectrometry (HPLC-ESI(+)-MS/MS) procedure was developed and validated for the identification and quantification of thevetin B and further cardiac glycosides in human serum. The seeds of Yellow Oleander (Thevetia peruviana) contain cardiac glycosides that can ...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2011.02.013
更新日期:2012-02-10 00:00:00
abstract::Forensic samples are commonly influenced by various environmental factors, including ultraviolet (UV) irradiation; thus, forensic applications of DNA repair (e.g., PreCR™, Restorase®) have been investigated, focusing on short tandem repeat typing. However, current DNA-based examinations are used for both human and bod...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2019.110077
更新日期:2020-01-01 00:00:00
abstract::Clinical and research-based tests in molecular biology require a substantial amount of DNA and RNA, unfortunately, a considerable number of cells is needed for this amount of sample. Blood is one of the best and easiest source of cells, but is not used due to its invasive drawing methods and the needed volume. Another...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2019.109931
更新日期:2019-10-01 00:00:00
abstract::In this paper, we describe an acetylating method for fast synthesis of heroin from morphine in the presence of 4-dimethylaminopyridine (4-DMAP) as a catalyst. In the reaction which led to heroin formation, the morphine base was subjected to a solution made up of 4-DMAP (catalyst), methylene chloride (solvent) and acet...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/s0379-0738(02)00291-8
更新日期:2002-10-09 00:00:00