Abstract:
:Diosgenin possesses anti-inflammatory and anticancer properties. Activated neutrophils produce high concentrations of the superoxide anion which is involved in the pathophysiology of inflammation-related diseases and cancer. In the present study, the inhibitory effect and possible mechanisms of diosgenin on superoxide generation were investigated in mouse bone marrow neutrophils. Diosgenin potently and concentration-dependently inhibited the extracellular and intracellular superoxide anion generation in Formyl-Met-Leu-Phe (FMLP)- activated neutrophils, with IC50 values of 0.50 ± 0.08 μM and 0.66 ± 0.13 μM, respectively. Such inhibition was not mediated by scavenging the superoxide anion or by a cytotoxic effect. Diosgenin inhibited the phosphorylation of p47phox and membrane translocation of p47phox and p67phox, and thus blocking the assembly of nicotinamide adenine dinucleotide phosphate oxidase. Moreover, cellular cyclic adenosine monophosphate (cAMP) levels and protein kinase A (PKA) expression were also effectively increased by diosgenin. It attenuated FMLP-induced increase of phosphorylation of cytosolic phospholipase A (cPLA2), p21-activated kinase (PAK), Akt, p38 mitogen-activated protein kinase (p38MAPK), extracellular signal-regulated kinase (ERK1/2), and c-Jun N-terminal kinase (JNK). Our data indicate that diosgenin exhibits inhibitory effects on superoxide anion production through the blockade of cAMP, PKA, cPLA2, PAK, Akt and MAPKs signaling pathways. The results may explain the clinical implications of diosgenin in the treatment of inflammation-related disorders.
journal_name
Free Radic Resjournal_title
Free radical researchauthors
Lin Y,Jia R,Liu Y,Gao Y,Zeng X,Kou J,Yu Bdoi
10.3109/10715762.2014.966705subject
Has Abstractpub_date
2014-12-01 00:00:00pages
1485-93issue
12eissn
1071-5762issn
1029-2470journal_volume
48pub_type
杂志文章abstract::Amphotericin B has been the gold standard for the treatment of invasive mycosis for many years. Its resistance mechanisms are reported to be mainly related to the decrease of ergosterol content or the changes of cell wall. Previous study has shown that Saccharomyces cerevisiae strain lack of BSC2 was sensitive signifi...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715762.2020.1751151
更新日期:2020-04-01 00:00:00
abstract::The purpose of this double clinical study was (1) to evaluate the effect of one single intake (300 ml) of red wine (RW) on the plasma antioxidant capacity (pAOC) and plasma phenolics over the 24-h time period following the intake, and (2) to compare the long-term effects of daily intakes (250 ml/d) of RW, white wine (...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760310001598097
更新日期:2003-09-01 00:00:00
abstract::In cells, NADH and NADPH are mainly bound to dehydrogenases such as lactate dehydrogenase (LDH). In cell-free systems, the binary LDH-NADH complex has been demonstrated to produce reactive oxygen species via a chain oxidation of NADH initiated and propagated by superoxide. We studied here whether this chain radical re...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760500231786
更新日期:2005-10-01 00:00:00
abstract::Oxidative stress is suggested as a potential mechanism in impaired foetal growth, smaller birth size and thus subsequently adult chronic diseases. We have investigated associations between oxidative stress in pregnancy and birth anthropometry (weight, height, head and chest circumferences). In the MINIMat-trial (Mater...
journal_title:Free radical research
pub_type: 临床试验,杂志文章
doi:10.3109/10715762.2011.651467
更新日期:2012-03-01 00:00:00
abstract::The concentration of taurine is high in the lens. However, its function therein remains unknown. Studies from other tissues suggest that in addition to several other modes of action, it acts as an antioxidant. We therefore hypothesize that taurine may be a part of the antioxidant defense mechanisms involved in protect...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715769800300221
更新日期:1998-09-01 00:00:00
abstract::Glycation of proteins or other biomolecules and their further long-term degradation result in the formation of advanced glycation end products, AGEs. AGEs and other ligands interact with their receptors, RAGEs, localized to a variety of tissues, but mainly in endothelium and vascular wall cells. This interaction trigg...
journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.3109/10715762.2013.815347
更新日期:2013-08-01 00:00:00
abstract::The primary clinical symptom of Japanese bovine theileriosis, caused by the intraerythrocytic protozoan Theileria sergenti, is anemia, but the underlying mechanism of this anemia remains unknown. To elucidate the pathogenesis of anemia developing in bovine theileriosis, we investigated the relationship between oxidati...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760310001607023
更新日期:2003-11-01 00:00:00
abstract::Macrophages produce reactive oxygen species such as O2-, H2O2 and *OH that contribute to the pathogenesis of diseases such as inflammation and atherosclerosis. The cells have multiple defense systems against those reactive oxygen species, and we describe here such an oxidative stress-inducible defense system. Upon exp...
journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.1080/10715769900300921
更新日期:1999-10-01 00:00:00
abstract::Although the primary biological function of retinal photoreceptors is to absorb light and provide visual information, extensive exposure to intense light could increase the risk of phototoxic reactions mediated by products of rhodopsin bleaching that might accumulate in photoreceptor outer segments (POS). The phototox...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715762.2019.1603377
更新日期:2019-04-01 00:00:00
abstract::To define the molecular mechanism(s) of carvedilol inhibition of lipid peroxidation we have utilized model systems that allow us to study the different reactions involved in this complex process. Carvedilol inhibits the peroxidation of sonicated phosphatidylcholine liposomes triggered by FeCl2 addition whereas atenolo...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715769800300421
更新日期:1998-11-01 00:00:00
abstract::Globular adiponectin (gAd) induces the generation of reactive oxygen species (ROS) and nitric oxide (NO) in the murine macrophage cell line RAW 264. This study investigated the role of the mammalian target of rapamycin (mTOR) in gAd-induced ROS and NO generation. gAd stimulation induced phosphorylation of mTOR, which ...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715760903348328
更新日期:2010-02-01 00:00:00
abstract::Generation of toxic oxygen metabolites followed by oxidant- and inflammatory-mediated tissue injury plays a crucial role in the pathogenesis of ischemia and reperfusion (IR). Ubiquinol, the reduced form of coenzyme Q10, is recognized for its potent antioxidant and anti-inflammatory properties in biological membranes. ...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2013.858148
更新日期:2014-02-01 00:00:00
abstract::Effects of vitamins B, C, E, K and P, as well as coenzymes Q, on formation of final products of radiation-induced free-radical transformations of ethanol, ethylene glycol, alpha-methylglycoside and glucose in aqueous solutions were studied. Based on the obtained results, it can be concluded that there are substances a...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760500147479
更新日期:2005-07-01 00:00:00
abstract::Melatonin is an endogenous indolamine, classically known as a light/dark regulator. Besides classical functions, melatonin has also showed to have a wide range of antitumoral effects in numerous cancer experimental models. However, no definite mechanism has been described to explain the whole range of antineoplasic ef...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2011.623700
更新日期:2011-11-01 00:00:00
abstract::Uraemic subjects undergoing chronic haemodialysis show increased oxidative stress. The use of non-biocompatible filters and reduced antioxidative defences are important sources of reactive oxygen species (ROS) release. The highly oxidative environment accelerates the onset and progression of tissue damage and atherosc...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760500445121
更新日期:2006-02-01 00:00:00
abstract::Araucaria angustifolia (Bert.) O. Kuntze is a species critically endangered of extinction and its development and propagation is strongly affected by abiotic stress. We have previously shown the activation of uncoupling protein in A. angustifolia embryogenic stem cells subjected to cold stress. Now, we have furthered ...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715762.2018.1548767
更新日期:2019-01-01 00:00:00
abstract::APE1/Ref-1, the mammalian ortholog of E. coli Xth, and a multifunctional protein possessing both DNA repair and transcriptional regulatory activities, has dual role in controlling cellular response to oxidative stress. It is rate-limiting in repair of oxidative DNA damage including strand breaks and also has co-transc...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760701765616
更新日期:2008-01-01 00:00:00
abstract::MCI-186 (3-methyl-1-phenyl-2-pyrazolin-5-one) is a newly developed antioxidant which has been shown to reduce brain edema in cerebral ischemia through inhibition of the lipoxygenase pathway of arachidonic acid. However, its effect on myocardial reperfusion injury after prolonged ischemia has not yet been demonstrated....
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715769609088034
更新日期:1996-05-01 00:00:00
abstract::Levels of certain antioxidants namely reduced glutathione (GSH), ascorbic acid (Vit C), alpha-tocopherol (Vit E) and antioxidant enzymes, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione reductase (GR) were compared in monkey and rat brain microvessels which constitute the blood...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715769509145642
更新日期:1995-04-01 00:00:00
abstract::Global gene expression profiles of livers from mice, fed diets differing in alpha-tocopherol content, were compared using DNA microarray technology. Three hundred and eighty nine genes were found to significantly differ in their expression level by a factor of 2 or higher between the high and the low alpha-tocopherol ...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760701416988
更新日期:2007-08-01 00:00:00
abstract::Oxidative reactions are thought to play a role in the inflammatory condition called fatty liver. It is unclear whether oxidized lipoprotein lipids or proteins are associated with future fatty liver. In the Cardiovascular Risk in Young Finns Study, we determined the circulating levels of LDL and HDL oxidized lipids and...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2015.1133906
更新日期:2016-01-01 00:00:00
abstract::Microcystin-LR (MCLR) is a potent hepatotoxin. Oxidative stress is thought to be implicated in the cytotoxicity of MCLR, but the mechanisms by which MCLR produces reactive oxygen species (ROS) are still unclear. This study investigated the role and possible sources of ROS generation in MCLR-induced cytogenotoxicity in...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760701704599
更新日期:2007-12-01 00:00:00
abstract::γ-Tocopherol (γ-T) scavenges reactive nitrogen species (RNS) to form 5-NO2-γ-T (NGT). However, α-T supplementation decreases circulating γ-T, which could limit its RNS scavenging activities. We hypothesized that α-T supplementation would mitigate NGT accumulation by impairing γ-T status. Healthy smokers (21 ± 1 y, n =...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2015.1040788
更新日期:2015-01-01 00:00:00
abstract::Radicals from one-electron oxidation of 4-hydroxycinnamate, ferulate and 3,4-dihydroxycinnamate have been formed by reaction with the oxidising triplet state of duroquinone. All three compounds react with triplet duroquinone with second order rate constants close to the diffusion-controlled limit. The identity of the ...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760100300621
更新日期:2001-07-01 00:00:00
abstract::Obesity enhances the frequency and severity of acute kidney injury (AKI). Telmisartan pre-treatment was used experimentally in the amelioration of ischemia/reperfusion (IR)-induced AKI. However, there is a lack of evidence regarding its beneficial effects on AKI in obese animals. The present study, therefore, aimed to...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715762.2016.1211644
更新日期:2016-09-01 00:00:00
abstract::Silver nanoparticles (AgNPs) are the most widely used nanomaterials as antimicrobial agents. AgNPs have been shown to inhibit the growth of and induce apoptosis-like death in Escherichia coli. However, the precise mechanism of AgNPs-induced apoptosis-like death and association with DNA damage-inducible protein F (dinF...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715762.2020.1866178
更新日期:2021-01-06 00:00:00
abstract::Polystyrene supported fluorinated cyclic nitrone spin-traps: Resin-2-HFDMPO (2-hydroxymethyl-2-methyl-5-(trifluoromethyl)-3,4-dihydro-2H-pyrrole-1-oxide) and Resin-2-PFDMPO (2-(3-hydroxypropyl)-2-methyl-5-(trifluoromethyl)-3,4-dihydro-2H-pyrrole 1-oxide) containing a trifluoromethyl pyrroline-N-oxide core were develop...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715762.2019.1683171
更新日期:2019-12-01 00:00:00
abstract::To solve the problems faced in clinical use of hypocrellins, a water-soluble preparation of Hypocrellin B (HB), HB-Triton X-100 (TX-100) micelles, was prepared. To evaluate the photodynamic activity, the free radicals (*OH and HB*-) and singlet oxygen (1O2) generated via photosensitization of the preparation in aqueou...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760310001601056
更新日期:2003-10-01 00:00:00
abstract::Oxidative stress appears to be implicated in the pathogenesis of various diseases including hepatotoxicity. Although intracellular Ca2+ signals have been suggested to play a role in the oxidative damage of hepatocytes, the sources and effects of oxidant-induced intracellular Ca2+ increases are currently debatable. Thu...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760000301431
更新日期:2000-09-01 00:00:00
abstract::Over the last decades, oxidative stress has been described as a deleterious phenomenon contributing to numerous noncommunicable diseases such as cardiovascular disease, diabetes, and cancers. As many authors ascribed the healthy effect of fruit and vegetable consumption mainly to their antioxidant contents, it has bee...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2013.853877
更新日期:2013-12-01 00:00:00