Abstract：
OBJECTIVE:Ursodeoxycholic acid (UDCA) is widely used to treat chronic liver diseases, and its cytoprotective effect on normal hepatocytes has been shown. This study aimed to investigate the apoptotic effects of UDCA on hepatocellular carcinoma (HCC) cells and the underlying molecular events in vitro. METHODS:HCC cells were treated by UDCA at different doses and periods of time to assess cell morphology, viability, apoptosis and gene expression using methyl thiazolyl tetrazolium (MTT), Annexin V/propidium iodide (PI) stain, transferase dUTP nick end labeling (TUNEL), enzyme-linked immunosorbent assay (ELISA), immunocytochemistry and quantitative reverse transcription polymerase chain reaction, respectively. RESULTS:UDCA treatment reduced cell viability but induced HCC cell apoptosis in dose-dependent and time-dependent manners. UDCA arrested HepG2 cells at phase S of the cell cycle. At the gene levels, UDCA downregulated Bcl-2 and second mitochondria-derived activator of caspase (Smac) protein expressions, but upregulated Bax and Livin proteins in HCC cells. At the highest concentration, UDCA inhibited Livin mRNA expression but increased Smac and caspase-3 mRNA expressions as well as the activity of caspase-3 in HCC cells. CONCLUSIONS:The induction of HCC cell apoptosis by UDCA was dose-dependent and time-dependent and was mediated by the regulation of Bax to Bcl-2 ratio, the expressions of Smac and Livin, and caspase-3 expression and activity.
journal_name
J Dig Disjournal_title
Journal of digestive diseasesauthors
Zhu L,Shan LJ,Liu YJ,Chen D,Xiao XG,Li Ydoi
10.1111/1751-2980.12191subject
Has Abstractpub_date
2014-12-01 00:00:00pages
684-93issue
12eissn
1751-2972issn
1751-2980journal_volume
15pub_type
杂志文章abstract：:Gastric cancer (GC) is one of the inflammation-associated cancers. Helicobacter pylori is now thought to be responsible for more than 95% of all GCs, and its development is associated with at least four mechanisms that lead to genetic instability of the gastric mucosa. The risk of developing GC can be predicted by ass...
journal_title：Journal of digestive diseases
pub_type： 杂志文章,评审
doi：10.1111/1751-2980.12105
更新日期：2014-01-01 00:00:00
abstract：:The incidence and prevalence of inflammatory bowel disease (IBD) show considerable variation over time and across geographical regions. The first studies on the epidemiology of IBD were mainly from traditionally high-incidence areas, such as North America, and northern and western Europe. In the last two decades, more...
journal_title：Journal of digestive diseases
pub_type： 杂志文章,评审
doi：10.1111/1751-2980.12449
更新日期：2017-02-01 00:00:00
abstract：OBJECTIVE:Probiotics are increasingly advocated in the management of various gastrointestinal disorders. The aim of this study was to investigate the current attitudes and prescribing practices of surgeons and gastroenterologists for probiotics in the treatment of gastrointestinal disorders. METHODS:A questionnaire wa...
journal_title：Journal of digestive diseases
pub_type： 杂志文章
doi：10.1111/j.1751-2980.2011.00534.x
更新日期：2011-12-01 00:00:00
abstract：OBJECTIVE:This study aimed to systematically assess the relevant risk factors of positive lateral margin (LM) after en bloc resection of early gastric adenocarcinoma. METHODS:A total of 242 patients who had undergone endoscopic submucosal dissection (ESD) due to early gastric lesions from January 2009 to March 2015 we...
journal_title：Journal of digestive diseases
pub_type： 杂志文章
doi：10.1111/1751-2980.12342
更新日期：2016-04-01 00:00:00
abstract：OBJECTIVE:To evaluate the long-term outcomes of complete common bile duct (CBD) stone removal and biliary stenting in elderly patients (≥85 years) with CBD stones. METHODS:We retrospectively examined 65 patients who underwent complete CBD stone removal (the duct clearance group) and 40 patients who underwent biliary s...
journal_title：Journal of digestive diseases
pub_type： 杂志文章
doi：10.1111/1751-2980.12656
更新日期：2018-10-01 00:00:00
abstract：OBJECTIVE:Hypomethylation within the body of the p53 gene, which is normally methylated, has been found in neoplasms. Also, the CG → TA transition was not detected in the CpG codons of the p53 gene in gastritis lesions in Iranian patients. Therefore, an evaluation of the probable correlation between global genome methy...
journal_title：Journal of digestive diseases
pub_type： 杂志文章
doi：10.1111/1751-2980.12053
更新日期：2013-06-01 00:00:00
abstract：OBJECTIVE:To investigate the alterations and functions of intrahepatic B (IHB) cells in non-alcoholic fatty liver disease (NAFLD) model induced by high-fat diet (HFD). METHODS:C57BL/6 J mice were fed with HFD for 16 weeks to induce NAFLD. Flow cytometry was used to analyze lymphocytes from liver, spleen and peripheral...
journal_title：Journal of digestive diseases
pub_type： 杂志文章
doi：10.1111/1751-2980.12362
更新日期：2016-07-01 00:00:00
abstract：OBJECTIVE:It is known that the vacuolating cytotoxin (VacA) could induce apoptosis. However, the mechanism remained to be elucidated. The aim of this study is to investigate the role of Bcl family of proteins (Bcl-2 and Bax) and the mitochondrial voltage-dependent anion channel (VDAC) in VacA-induced apoptosis of AGS c...
journal_title：Journal of digestive diseases
pub_type： 杂志文章
doi：10.1111/j.1751-2980.2009.00412.x
更新日期：2010-02-01 00:00:00
abstract：OBJECTIVE:To explore the prevalence of and risk factors for gallstone disease in Shanghai, China. METHODS:A population-based cross-sectional study was conducted in Shanghai between 2016 and 2017. Using a three-stage stratified sampling strategy, 4009 participants (1753 men and 2256 women) from 10 districts were enroll...
journal_title：Journal of digestive diseases
pub_type： 杂志文章
doi：10.1111/1751-2980.12857
更新日期：2020-04-01 00:00:00
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journal_title：Journal of digestive diseases
pub_type： 杂志文章
doi：10.1111/j.1751-2980.2011.00550.x
更新日期：2012-01-01 00:00:00
abstract：OBJECTIVE:To determine whether computer-predicted short RNA structural analogues could inhibit hepatitis C virus (HCV) genotype 2a, 3a and 4a replication in cultured cells. METHODS:Short RNA sequences, X12, X12a and X12b, designed to be identical in secondary structure to the X region in the 3'-untranslated region (3'...
journal_title：Journal of digestive diseases
pub_type： 杂志文章
doi：10.1111/1751-2980.12250
更新日期：2015-08-01 00:00:00
abstract：OBJECTIVE:This study aimed to compare the diagnostic value and image quality of high-definition magnifying white light endoscopy (M-WLE) and high-definition magnifying endoscopy with i-scan (M-i-scan) for Helicobacter pylori (H.pylori) infection. METHODS:Patients complaining of dyspepsia who underwent esophagogastrodu...
journal_title：Journal of digestive diseases
pub_type： 杂志文章
doi：10.1111/1751-2980.12086
更新日期：2013-11-01 00:00:00
abstract：OBJECTIVE:The aim of our study was to document our 6-year experiences in identifying the clinical characteristics, laboratory findings, risk factors and the outcomes of patients with ischemic colitis (IC) in a community hospital setting. METHODS:The medical records of patients who were diagnosed with IC from 2007 to 2...
journal_title：Journal of digestive diseases
pub_type： 杂志文章
doi：10.1111/1751-2980.12182
更新日期：2014-11-01 00:00:00
abstract：OBJECTIVE:To explore the features and clinical significance of serum pepsinogen (PG) assay in a follow-up study on a high-risk gastric cancer (GC) population. METHODS:A total of 444 participants from a high-risk area of GC in north China were enrolled in this follow-up study from April 1997 to December 1999. Serum PG ...
journal_title：Journal of digestive diseases
pub_type： 杂志文章
doi：10.1111/j.1443-9573.2007.00321.x
更新日期：2008-02-01 00:00:00
abstract：:Non-alcoholic fatty liver disease (NAFLD) is now the most common etiology of chronic liver disease threatening global public health. However, the name "NAFLD" is no longer appropriate with the change of time. Recently, a new term, "metabolic dysfunction-associated fatty liver disease" has been proposed by an internati...
journal_title：Journal of digestive diseases
pub_type： 杂志文章
doi：10.1111/1751-2980.12944
更新日期：2020-11-01 00:00:00
abstract：OBJECTIVE:This study aimed to investigate the efficacy of fluorescence spectroscopy of gastric juice for early gastric cancer (EGC) screening. METHODS:Gastric juice was collected from 101 participants who underwent endoscopy in the Outpatient Endoscopy Center of Peking University Third Hospital. The participants were ...
journal_title：Journal of digestive diseases
pub_type： 杂志文章
doi：10.1111/1751-2980.12040
更新日期：2013-06-01 00:00:00
abstract：OBJECTIVES:To assess the prevalence and potential risk factors of latent tuberculosis infection (LTBI) in Chinese patients with inflammatory bowel disease (IBD) and to evaluate the role of chest computed tomography (CT) in the screening of LTBI. METHODS:A single-center retrospective study was conducted and all IBD pat...
journal_title：Journal of digestive diseases
pub_type： 杂志文章
doi：10.1111/1751-2980.12437
更新日期：2017-01-01 00:00:00
abstract：:The development of alcoholic liver disease (ALD) is a complex process involving both the parenchymal and non-parenchymal cells in the liver. The impact of ethanol on hepatocytes can be characterized as a condition of organelle stress with multifactorial changes in hepatocellular function accumulating during ethanol ex...
journal_title：Journal of digestive diseases
pub_type： 杂志文章,评审
doi：10.1111/j.1751-2980.2010.00468.x
更新日期：2011-02-01 00:00:00
abstract：OBJECTIVE:Currently the screening and diagnosis of gastric cancer and atrophic gastritis are mainly made by endoscopy and biopsy. The aim of this study was to evaluate the use of serum tests: serum pepsinogen I (PGI pepsinogen I/II ratio (PGR), gastrin-17 (G-17) and H. pylori-immunoglobulin G (IgG) antibodies to screen...
journal_title：Journal of digestive diseases
pub_type： 杂志文章
doi：10.1111/j.1443-9573.2007.00271.x
更新日期：2007-02-01 00:00:00
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journal_title：Journal of digestive diseases
pub_type： 杂志文章
doi：10.1111/1751-2980.12082
更新日期：2013-10-01 00:00:00
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journal_title：Journal of digestive diseases
pub_type： 杂志文章
doi：10.1111/j.1751-2980.2008.00346.x
更新日期：2008-11-01 00:00:00
abstract：:Helicobacter pylori (H. pylori) infection causes peptic ulcer disease, mucosa-associated lymphoid tissue (MALT) lymphomas and gastric adenocarcinomas, for which the pathogenesis of chronic gastric inflammation prevails and provides the pathogenic basis. Since the role of H. pylori infection is promoting carcinogenesis...
journal_title：Journal of digestive diseases
pub_type： 杂志文章,评审
doi：10.1111/j.1751-2980.2008.00334.x
更新日期：2008-08-01 00:00:00
abstract：OBJECTIVE:Contrast-enhanced harmonic endoscopic ultrasonography (CH-EUS) is a valuable device to diagnose and determine the malignant potential of gastrointestinal stromal tumors (GIST) as early as possible when making clinical therapeutic decisions. This study aimed to estimate the ability of CH-EUS to discriminate be...
journal_title：Journal of digestive diseases
pub_type： 杂志文章,meta分析
doi：10.1111/1751-2980.12710
更新日期：2019-03-01 00:00:00
abstract：OBJECTIVE:Methotrexate (MTX) can be used as an alternative for patients with Crohn's disease (CD) who are intolerant of thiopurine. This retrospective study aimed to provide some clues about MTX treatment in Chinese patients with CD. METHODS:Medical records of 27 adult patients with CD who were treated with MTX betwee...
journal_title：Journal of digestive diseases
pub_type： 杂志文章
doi：10.1111/1751-2980.12694
更新日期：2018-12-01 00:00:00
abstract：:Inflammatory bowel disease (IBD) has long been considered a disease that affects predominantly a Western population. The incidence and prevalence rates from Asian populations are much lower in comparison. More recent data, however, have shown significantly higher rates in Asians and time trend studies have shown an in...
journal_title：Journal of digestive diseases
pub_type： 杂志文章,评审
doi：10.1111/j.1751-2980.2008.00355.x
更新日期：2009-02-01 00:00:00
abstract：OBJECTIVE:This study aimed to determine the prevalence of and risk factors associated with elevated alanine aminotransferase (ALT) levels among a sample of normal weight, overweight and obese youth from two urban populations in Central Mexico. METHODS:Baseline data from 1262 youth aged 8-19 years who participated in t...
journal_title：Journal of digestive diseases
pub_type： 杂志文章
doi：10.1111/1751-2980.12072
更新日期：2013-09-01 00:00:00
abstract：OBJECTIVE:To carry out a comprehensive study on gastrointestinal symptoms, motility and autonomic neuropathy in chronic alcoholics before and one year after abstinence. METHODS:Dyspeptic symptoms (questionnaires), fasting and postprandial gallbladder and gastric motility (ultrasonography), oro-cecal transit time (lact...
journal_title：Journal of digestive diseases
pub_type： 杂志文章
doi：10.1111/1751-2980.12415
更新日期：2016-11-01 00:00:00
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journal_title：Journal of digestive diseases
pub_type： 杂志文章,评审
doi：10.1111/1751-2980.12880
更新日期：2020-06-01 00:00:00
abstract：OBJECTIVE:To investigate the role of ghrelin in the gastric cancer cell line AGS and its probable mechanism. METHODS:Cell proliferation was detected by MTT assay after treated with ghrelin or des-acyl ghrelin. The expression of growth hormone secretagogue receptor 1a (GHS-R1a) and 1b (GHS-R1b) mRNA was detected using ...
journal_title：Journal of digestive diseases
pub_type： 杂志文章
doi：10.1111/j.1751-2980.2012.00616.x
更新日期：2012-09-01 00:00:00
abstract：OBJECTIVE:To explore the role of tagging single nucleotide polymorphisms (tagSNPs) in the adiponectin gene in the natural course of nonalcoholic fatty liver disease (NAFLD). METHODS:The participants were chosen from our previous survey containing 3543 individuals. Finally, a total of 696 participants who had been foll...
journal_title：Journal of digestive diseases
pub_type： 杂志文章
doi：10.1111/1751-2980.12288
更新日期：2015-10-01 00:00:00
