Abstract:
:Homologous recombination is a molecular process that has multiple important roles in DNA metabolism, both for DNA repair and genetic variation in all forms of life. Generally, homologous recombination involves the exchange of genetic information between two identical or nearly identical DNA molecules; however, homologous recombination can also occur between RNA molecules, as shown for RNA viruses. Previous research showed that synthetic RNA oligonucleotides can act as templates for DNA double-strand break (DSB) repair in yeast and human cells, and artificial long RNA templates injected in ciliate cells can guide genomic rearrangements. Here we report that endogenous transcript RNA mediates homologous recombination with chromosomal DNA in yeast Saccharomyces cerevisiae. We developed a system to detect the events of homologous recombination initiated by transcript RNA following the repair of a chromosomal DSB occurring either in a homologous but remote locus, or in the same transcript-generating locus in reverse-transcription-defective yeast strains. We found that RNA-DNA recombination is blocked by ribonucleases H1 and H2. In the presence of H-type ribonucleases, DSB repair proceeds through a complementary DNA intermediate, whereas in their absence, it proceeds directly through RNA. The proximity of the transcript to its chromosomal DNA partner in the same locus facilitates Rad52-driven homologous recombination during DSB repair. We demonstrate that yeast and human Rad52 proteins efficiently catalyse annealing of RNA to a DSB-like DNA end in vitro. Our results reveal a novel mechanism of homologous recombination and DNA repair in which transcript RNA is used as a template for DSB repair. Thus, considering the abundance of RNA transcripts in cells, RNA may have a marked impact on genomic stability and plasticity.
journal_name
Naturejournal_title
Natureauthors
Keskin H,Shen Y,Huang F,Patel M,Yang T,Ashley K,Mazin AV,Storici Fdoi
10.1038/nature13682subject
Has Abstractpub_date
2014-11-20 00:00:00pages
436-9issue
7527eissn
0028-0836issn
1476-4687pii
nature13682journal_volume
515pub_type
杂志文章相关文献
NATURE文献大全abstract::Recent studies have shown that developing nerve fibres grow directly to their targets and are guided by specific cues, but the nature of these cues and the mechanism of guidance remain unknown. The growth of sympathetic axons towards an artificial source of nerve growth factor (NGF) in vivo and of sensory neurites up ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/306786a0
更新日期:1983-12-22 00:00:00
abstract::A putative growth hormone receptor from rabbit liver and the growth hormone binding protein from rabbit serum have the same amino-terminal amino-acid sequence, indicating that the binding protein corresponds to the extracellular hormone-binding domain of the liver receptor. The complete amino-acid sequences derived fr...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/330537a0
更新日期:1987-12-10 00:00:00
abstract::Locomotion relies on neural networks called central pattern generators (CPGs) that generate periodic motor commands for rhythmic movements. In vertebrates, the excitatory synaptic drive for inducing the spinal CPG can originate from either supraspinal glutamatergic inputs or from within the spinal cord. Here we identi...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature08323
更新日期:2009-09-17 00:00:00
abstract::A 12 amino-acid synthetic peptide (NANP)3 comprising the immunodominant epitope of Plasmodium falciparum circumsporozoite protein was conjugated to tetanus toxoid (TT), adjuvanted with aluminium hydroxide, and administered intramuscularly in three doses at monthly intervals to 35 healthy males as a malaria vaccine. No...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/328257a0
更新日期:1987-07-16 00:00:00
abstract::Phytoplankton size structure is acknowledged as a fundamental property determining energy flow through 'microbial' or 'herbivore' pathways. The balance between these two pathways determines the ability of the ecosystem to recycle carbon within the upper layer or to export it to the ocean interior. Small cells are usua...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/35066560
更新日期:2001-03-15 00:00:00
abstract::The 30S ribosomal subunit binds messenger RNA and the anticodon stem-loop of transfer RNA during protein synthesis. A crystallographic analysis of the structure of the subunit from the bacterium Thermus thermophilus is presented. At a resolution of 5.5 A, the phosphate backbone of the ribosomal RNA is visible, as are ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/23631
更新日期:1999-08-26 00:00:00
abstract::One approach to the controlled release of drugs involves incorporation of the drug molecules into the matrix of microscopic polymer spheres or capsules. Existing methods for preparing such microparticles do not, however, always guarantee a constant release rate, for example because drug molecules may be trapped prefer...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/367258a0
更新日期:1994-01-20 00:00:00
abstract::Throughout the latter half of this century, the development and spread of resistance to most front-line antimalarial compounds used in the prevention and treatment of the most severe form of human malaria has given cause for grave clinical concern. Polymorphisms in pfmdr1, the gene encoding the P-glycoprotein homologu...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/35002615
更新日期:2000-02-24 00:00:00
abstract::The three-dimensional structure of intact human rhinovirus 14 (HRV-14) complexed with Fab fragments (Fab17-IA) from a strongly neutralizing antibody that binds bivalently to the virion has been determined to 4.0 angstrom resolution by a combination of X-ray crystallography and cryo-electron microscopy. In contradictio...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/383350a0
更新日期:1996-09-26 00:00:00
abstract::Mice of the AKR strain are characterised by a high incidence of spontaneous thymic lymphomas. AKR chromosomes contain the genomes of ecotropic murine leukaemia virus (MuLV) at two loci, termed Akv-1 and Akv-2 (refs 2-6). Shortly after birth, the normal tissues of AKR mice begin to produce high levels of this XC-positi...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/282753a0
更新日期:1979-12-13 00:00:00
abstract::The Hawaiian-Emperor volcanic island and seamount chain is usually attributed to a hot mantle plume, located beneath the Pacific lithosphere, that delivers material sourced from deep in the mantle to the surface. The shield volcanoes of the Hawaiian islands are distributed in two curvilinear, parallel trends (termed '...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature03907
更新日期:2005-08-11 00:00:00
abstract::The masses of the supermassive black holes found in galaxy bulges are correlated with a multitude of galaxy properties, leading to suggestions that galaxies and black holes may evolve together. The number of reliably measured black-hole masses is small, and the number of methods for measuring them is limited, holding ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature11819
更新日期:2013-02-21 00:00:00
abstract::Megakaryocytopoiesis is the cellular developmental process that leads to platelet production. At least two humoral growth factors may be necessary for megakaryocyte proliferation and maturation. One is a megakaryocyte-colony stimulating factor (MK-CSF) which induces the proliferation and differentiation of megakaryocy...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/369571a0
更新日期:1994-06-16 00:00:00
abstract::The complete knowledge of a quantum state allows the prediction of the probability of all possible measurement outcomes, a crucial step in quantum mechanics. It can be provided by tomographic methods which have been applied to atomic, molecular, spin and photonic states. For optical or microwave photons, standard tomo...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature13821
更新日期:2014-10-30 00:00:00
abstract::De novo engineering of gene circuits inside cells is extremely difficult, and efforts to realize predictable and robust performance must deal with noise in gene expression and variation in phenotypes between cells. Here we demonstrate that by coupling gene expression to cell survival and death using cell-cell communic...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature02491
更新日期:2004-04-22 00:00:00
abstract::Reorganizations of the Atlantic meridional overturning circulation were associated with large and abrupt climatic changes in the North Atlantic region during the last glacial period. Projections with climate models suggest that similar reorganizations may also occur in response to anthropogenic global warming. Here I ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature03476
更新日期:2005-03-31 00:00:00
abstract::This corrects the article DOI: 10.1038/nature24295. ...
journal_title:Nature
pub_type: 杂志文章,已发布勘误
doi:10.1038/nature25017
更新日期:2018-01-25 00:00:00
abstract:: ...
journal_title:Nature
pub_type: 评论,新闻
doi:10.1038/d41586-019-00761-2
更新日期:2019-03-01 00:00:00
abstract::Overfishing of large-bodied benthic fishes and their subsequent population collapses on the Scotian Shelf of Canada's east coast and elsewhere resulted in restructuring of entire food webs now dominated by planktivorous, forage fish species and macroinvertebrates. Despite the imposition of strict management measures i...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature10285
更新日期:2011-07-27 00:00:00
abstract::In legumes, root nodule organogenesis is activated in response to morphogenic lipochitin oligosaccharides that are synthesized by bacteria, commonly known as rhizobia. Successful symbiotic interaction results in the formation of highly specialized organs called root nodules, which provide a unique environment for symb...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature01207
更新日期:2002-11-28 00:00:00
abstract::Initial studies of somatically acquired mutations in immunoglobulin V regions from hybridomas and myelomas that are not derived from joining aberrations, suggested a controlled and specific hypermutation process, because spontaneous mutation rates observed for other genes are extremely low. Some evidence for the idea ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/326405a0
更新日期:1987-03-26 00:00:00
abstract::The origins of the building blocks of the Solar System can be studied using the isotopic composition of early planetary and meteoritic material. Oxygen isotopes in planetary materials show variations at the per cent level that are not related to the mass of the isotopes; rather, they result from the mixture of compone...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature03947
更新日期:2005-09-15 00:00:00
abstract::How environmental cues regulate adult stem cell and cancer cell activity through surface receptors is poorly understood. Angiopoietin-like proteins (ANGPTLs), a family of seven secreted glycoproteins, are known to support the activity of haematopoietic stem cells (HSCs) in vitro and in vivo. ANGPTLs also have importan...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature11095
更新日期:2012-05-30 00:00:00
abstract::Betelgeuse, a nearby red supergiant, is a fast-moving star with a powerful stellar wind that drives a bow shock into its surroundings. This picture has been challenged by the discovery of a dense and almost static shell that is three times closer to the star than the bow shock and has been decelerated by some external...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature13522
更新日期:2014-08-21 00:00:00
abstract::The fruit, which mediates the maturation and dispersal of seeds, is a complex structure unique to flowering plants. Seed dispersal in plants such as Arabidopsis occurs by a process called fruit dehiscence, or pod shatter. Few studies have focused on identifying genes that regulate this process, in spite of the agronom...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/35008089
更新日期:2000-04-13 00:00:00
abstract::Hepatocellularcarcinoma (HCC) that occur in woodchucks chronically infected with woodchuck hepatitis virus (WHV) were screened for activation of cellular oncogenes. Enhanced expression and allelic alterations of the c-myc oncogene were found in three HCC out of nine. Variations in the size of the c-myc transcripts, ra...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/324276a0
更新日期:1986-11-20 00:00:00
abstract::It has long been known that the 5' to 3' polarity of DNA synthesis results in both a leading and lagging strand at all replication forks. Until now, however, there has been no evidence that leading or lagging strands are spatially organized in any way within a cell. Here we show that chromosome segregation in Escheric...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature07282
更新日期:2008-10-30 00:00:00
abstract::A large number of cis-regulatory sequences have been annotated in the human genome, but defining their target genes remains a challenge. One strategy is to identify the long-range looping interactions at these elements with the use of chromosome conformation capture (3C)-based techniques. However, previous studies lac...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature12644
更新日期:2013-11-14 00:00:00
abstract::T200, a major cell-surface glycoprotein on lymphoid cells, exists in several forms with different electrophoretic mobilities. These forms have been correlated with different classes of lymphoid cell. The smaller forms, with molecular weights (MWs) of congruent to 170,000 and 180,000 are found predominantly on T cells ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/289681a0
更新日期:1981-02-19 00:00:00
abstract::The mineral olivine dominates the composition of the Earth's upper mantle and hence controls its mechanical behaviour and seismic anisotropy. Experiments at high temperature and moderate pressure, and extensive data on naturally deformed mantle rocks, have led to the conclusion that olivine at upper-mantle conditions ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature03266
更新日期:2005-02-17 00:00:00