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Effects of Schisandra chinensis extract on the relaxation of isolated human prostate tissue and smooth muscle cell.

Abstract：

ETHNOPHARMACOLOGICAL RELEVANCE:Schisandra chinensis has been commonly used as a traditional herbal medicine to treat various diseases including body weakness, dysentery, impotence, enuresis and frequent urination in many countries including Korea, China and Russia. Benign prostate hyperplasia is a common disease for the elderly men and it induces lower urinary tract symptoms which hinder general activity and quality of life. We evaluated the therapeutic potential of Schisandra chinensis extract (SCE) in benign prostate hyperplasia using human prostate tissue. MATERIALS AND METHODS:Schisandra chinensis fruit was collected and extracted with ethanol. Human prostate tissues were obtained from 14 prostate cancer patients. Macroscopically normal tissue was excised from the transition zone and the periurethral regions. Isolated prostate tissue strips were mounted in an organ-bath system, and the relaxation effect of SCE was evaluated by cumulative addition to prostate strips pre-contracted with 10(-5)M norepinephrine. The effect of tamsulosin was compared, and the additive effect was evaluated. Electrophysiological studies using cultured human prostate smooth muscle cells (HPrSMC) were conducted. RESULTS:Cumulative dosing of SCE induced concentration-dependent relaxation in contracted prostate tissue (n=18, P<0.05). Simultaneous dosing of SCE and tamsulosin showed an additive relaxation effect. The relaxation effect of SCE was abolished by inhibition of K+ channels by pre-treatment with tetraethylammonium. In HPrSMC, extracellular application of 100 μg/mL SCE significantly increased outward currents, and this effect was significantly attenuated by treatment with 100 nM Iberiotoxin. CONCLUSIONS:SCE showed a dose dependent relaxation effect on human prostate tissue as well as an additive effect with tamsulosin. The relaxation effects of SCE on HPrSMC were, in part, due to the activation of K+ channels.

journal_name

J Ethnopharmacol

journal_title

Journal of ethnopharmacology

authors

Choo SH,Sung HH,Chae MR,Kang SJ,Han DH,Park JK,So I,Lee SW

doi

10.1016/j.jep.2014.08.025

subject

Has Abstract

pub_date

2014-10-28 00:00:00

pages

271-6

eissn

0378-8741

issn

1872-7573

pii

S0378-8741(14)00619-9

journal_volume

156

pub_type

杂志文章

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Effects of Schisandra chinensis extract on the relaxation of isolated human prostate tissue and smooth muscle cell.