Abstract:
:This study was conducted to estimate the frequency of Cryptosporidium infections in Taif (Saudi Arabia). Stool samples from children under 10 years by modified Ziehl-Neelson staining and two PCR techniques were used for genotyping experiments. The microscopic examination showed that, eleven samples were positive for presence of Cryptosporidium. With 11 of 100 samples, DNA extraction and subsequent genotyping was successful. By means of RAPD technique, the genetic similarity among the collected isolates was 55%. The 18S rRNA gene sequences confirmed that all Cryptosporidium-isolates belonged to Cryptosporidium parvum. In comparison with reference strains from different species of Cryptosporidium species from GenBank, all collected isolates belonged to Cryptosporidium hominis and C. parvum clade. The fact that only human genotypes were detected suggests that cryptosporidiosis must primarily be considered as a non zoonotic disease in Taif region.
journal_name
Trop Biomedjournal_title
Tropical biomedicineauthors
Shalaby I,Gherbawy Y,Jamjoom M,Banaja Asubject
Has Abstractpub_date
2014-06-01 00:00:00pages
215-24issue
2eissn
0127-5720issn
2521-9855journal_volume
31pub_type
杂志文章abstract::In the present study, serum samples from 402 sheep and 216 goats were collected from 5 counties in Jinzhou from August to October 2012 and antibodies to Toxoplasma gondii were detected by modified agglutination test (MAT). Overall, 104 (16.8%) had antibodies to T. gondii with antibody titres of 1:25 to 1:800. Seroposi...
journal_title:Tropical biomedicine
pub_type: 杂志文章
doi:
更新日期:2015-09-01 00:00:00
abstract::Hand, foot and mouth disease (HFMD) is a common viral infection among infants and children. The major causative agents of HFMD are enterovirus 71 (EV71) and coxsackievirus A16 (CVA16). Recently, coxsackievirus A6 (CVA6) infections were reported in neighboring countries. Infected infants and children may present with f...
journal_title:Tropical biomedicine
pub_type: 杂志文章
doi:
更新日期:2014-12-01 00:00:00
abstract::The adulticidal activity of methanol extracts from three Malaysian plants namely Acorus calamus Linn., Litsea elliptica Blume and Piper aduncum Linn. against adult of Aedes aegypti (L.) were studied. Standard WHO bioassay tests were used to evaluate the effectiveness of these plant extracts. The hexane fraction from m...
journal_title:Tropical biomedicine
pub_type: 杂志文章
doi:
更新日期:2004-12-01 00:00:00
abstract::The determination of parasitemia in the diagnosis of malaria is a routine practice because it assists the selection of treatment. The techniques used for estimating parasitemia are based on leukocyte counts or on a fixed volume of blood examined in a microscopic field. This study evaluated the concordance between para...
journal_title:Tropical biomedicine
pub_type: 杂志文章
doi:
更新日期:2014-06-01 00:00:00
abstract::Analysis on fly artifacts produced by forensically important blowfly, Chrysomya megacephala (Fabricius) (Diptera:Calliphoridae), revealed several unique patterns. They can be divided into fecal spots, regurgitation spots and swiping stains. The characteristics of fecal spots are round with three distinct levels of pig...
journal_title:Tropical biomedicine
pub_type: 杂志文章
doi:
更新日期:2008-04-01 00:00:00
abstract::Dengue serotype surveillance is important as any changes in serotype distribution may result in an outbreak or increase in severe dengue cases. This study aimed to determine circulating dengue serotypes in two hospitals in Selangor. Serum samples were collected from patients admitted for dengue at these two major publ...
journal_title:Tropical biomedicine
pub_type: 杂志文章
doi:
更新日期:2015-03-01 00:00:00
abstract::Mosquitoes transmit serious human diseases, causing millions of deaths every year. Use of synthetic insecticides to control vector mosquitoes has caused physiological resistance and adverse environmental effects in addition to high operational cost. Insecticides of botanical origin may serve as suitable alternative bi...
journal_title:Tropical biomedicine
pub_type: 杂志文章
doi:
更新日期:2010-08-01 00:00:00
abstract::Infections and malignancies are common causes of pleural effusion. Among infectious causes, hyperinfection syndrome of Strongyloides stercoralis may occur in immunosuppressive patient. A 62-year-old man, known case of Non-Hodgkin lymphoma (NHL) was presented with recurrent NHL stage IV and had undergone salvage chemot...
journal_title:Tropical biomedicine
pub_type: 杂志文章
doi:
更新日期:2011-04-01 00:00:00
abstract::Crab is an important source of food allergen. Tropomyosin represents the main crab allergen and is responsible for IgE cross-reactivity between various species of crustaceans. Recently, other new crab allergens including arginine kinase have been identified. However, information on allergens of the local Portuni...
journal_title:Tropical biomedicine
pub_type: 杂志文章
doi:
更新日期:2012-09-01 00:00:00
abstract::This study assessed the effectiveness of a biorational control approach using 2% hydramethylnon gel bait on German cockroaches, Blattella germanica (L.) in some residential and hospital buildings in South Western Iran. In total, three buildings consisting of 150 apartment units and 101 hospital units were monitored we...
journal_title:Tropical biomedicine
pub_type: 杂志文章
doi:
更新日期:2010-08-01 00:00:00
abstract::Haematophagous dipterans belonging to 10 genera - Aedes (12), Anopheles (14), Armigeres (01), Culex (09), Toxorhynchites (01), Uranotaenia (01), Sergentomyia (02), Phlebotomus (01), Atylotus (01) and Tabanus (03) were encountered from 12 localities under 6 blocks of Dehradun district (Uttarakhand) during January 2011 ...
journal_title:Tropical biomedicine
pub_type: 杂志文章
doi:
更新日期:2013-12-01 00:00:00
abstract::Strongyloides stercoralis is a widespread, soil-transmitted helminth affecting humans. Autoinfection occurs in S. stercoralis infection and this leads to a continuous build-up of worm burden in human host. This may lead to hyperinfection syndrome which has the potential to cause serious life-threatening disease especi...
journal_title:Tropical biomedicine
pub_type: 杂志文章
doi:
更新日期:2010-04-01 00:00:00
abstract::Plaque assay procedure was carried out to determine the infectivity titration of dengue virus using Aedes albopictus C6/36 mosquito cell line. Cells were seeded in 6 wells plates and incubated until monolayer C6/36 cell lines were formed. Each well was exposed to a different dilution of dengue infected culture fluid, ...
journal_title:Tropical biomedicine
pub_type: 杂志文章
doi:
更新日期:2005-12-01 00:00:00
abstract::Nosocomial blood stream infection (or nosocomial bacteremia) is a common problem in hospitals worldwide, including Malaysia. A three-year prospective cohort study (October 2003-March 2007) of the incidences, risk factors, and patterns of the microorganisms causing bacteremia was conducted using a validated surveillanc...
journal_title:Tropical biomedicine
pub_type: 杂志文章
doi:
更新日期:2010-08-01 00:00:00
abstract::A total of 105 blood samples from healthy horses from different stables in Yazd province, center of Iran, were examined for the presence of Theileria equi infection using parasitological and molecular methods. Out of the 105 samples, the parasitological method detected T. equi infection in 5 (4.76%) cases while the PC...
journal_title:Tropical biomedicine
pub_type: 杂志文章
doi:
更新日期:2014-03-01 00:00:00
abstract::Sugarcane bagasse is a renewable resource that can be used to produce biopesticide for the control of mosquito vectors. In the present study, we demonstrated that cane processed bagasse could be used to produce Bacillus thuringiensis serovar israelensis (Bti) for control of mosquito vectors viz: Culex quinquefasciatus...
journal_title:Tropical biomedicine
pub_type: 杂志文章
doi:
更新日期:2013-09-01 00:00:00
abstract::We report a patient presenting with chronic diarrhoea of unidentified etiology. Laboratory results showed microcytic anemia, peripheral eosinophilia with negative results of stool sample smears and stool concentration technique. Ancylostoma duodenale was found in the caecum and terminal ileum during colonoscopy. The p...
journal_title:Tropical biomedicine
pub_type: 杂志文章
doi:
更新日期:2014-03-01 00:00:00
abstract::In the present study, a total of 1,235 porcine serum samples were collected from 9 counties in Jilin Province (40°52'~46°18'N,121°38'~131°19'E), northeastern China from August to October 2013, and the seroprevalence of Toxoplasma gondii infection was tested by indirect haemagglutination assay (IHA). The results showed...
journal_title:Tropical biomedicine
pub_type: 杂志文章
doi:
更新日期:2015-03-01 00:00:00
abstract::Using standard WHO methodology, this study investigated the susceptibility of 4(th) instar Aedes aegypti (L) and Culex annulirostris (Skuse) larvae to three extracts from Callitris glaucophylla (J. Thompson & L. Johnson) (1: steam distillation extract, 2: liquefied refrigerant gas extract, and 3: methanol reflux extra...
journal_title:Tropical biomedicine
pub_type: 杂志文章
doi:
更新日期:2006-12-01 00:00:00
abstract::This study explored the efficacy of Toxorhynchites splendens, predator of Aedes albopictus as a biocontrol agent. There was a negative correlation between Ae. albopictus larval population and Tx. splendens larval population in ovitraps (r=-0.287, R²=0.0821). The correlation is higher between the mean number of Ae. alb...
journal_title:Tropical biomedicine
pub_type: 杂志文章
doi:
更新日期:2011-08-01 00:00:00
abstract::Aim of present study was to screen medicinal plants for flukicidal activity in vitro to develop alternative sources of treatment for trematodes infection. For this purpose, crude methanolic extracts (CME) of Cymbopogn jwarancusa and Conyza canadensis were prepared and live adult flukes viz; Fasciola gigantica, and Par...
journal_title:Tropical biomedicine
pub_type: 杂志文章
doi:
更新日期:2015-09-01 00:00:00
abstract::Dengue virus (DV) infections are a serious public health problem and there is currently no vaccine or drug treatment. NS2B/NS3 protease, an essential enzyme for viral replication, is one of the promising targets in the search for drugs against DV. In this research work, virtual screening (VS) was carried out on four m...
journal_title:Tropical biomedicine
pub_type: 杂志文章
doi:
更新日期:2013-09-01 00:00:00
abstract::FT-IR spectra of liver tissue isolated from mice, Mus musculus, have been recorded in the region of 4000 - 400 cm-1 for normal, mercury treated and recovery phase. In this study, the total protein content was found to be decreased in the liver tissues after treatment with median-lethal dose of mercuric chloride. The m...
journal_title:Tropical biomedicine
pub_type: 杂志文章
doi:
更新日期:2005-06-01 00:00:00
abstract::A survey of cockroach fauna was carried out on the 3 inhabited islands of the Ogasawara chain island of Japan, namely, Chichijima island, Hahajima island and Iwo island. Seven species, namely, Periplaneta americana (Linnaeus, 1758), Periplaneta australasiae (Fabricius, 1775), Blattella lituricollis (Walker, 1868), Ony...
journal_title:Tropical biomedicine
pub_type: 杂志文章
doi:
更新日期:2013-03-01 00:00:00
abstract::Poultry farming not only provides high nutritious food but also creates employment opportunity for rural masses. Documented evidences elaborates that helminth parasitism is most deciduous problem of chickens especially in developing world. Ascaridia (A.) galli, a nematode of small intestine, has been considered as the...
journal_title:Tropical biomedicine
pub_type: 杂志文章
doi:
更新日期:2015-03-01 00:00:00
abstract::The disease melioidosis, caused by the soil bacteria Burkholderia pseudomallei, often manifests as acute septicemia with high fatality. Glycogen synthase kinase-3β (GSK3β) plays a key role during the inflammatory response induced by bacteria. We used a murine model of acute melioidosis to investigate the effects...
journal_title:Tropical biomedicine
pub_type: 杂志文章
doi:
更新日期:2012-12-01 00:00:00
abstract::Angiostrongylus cantonensis, the nematode lungworm of rats, has a XX/X0 sex-determination mechanism. The chromosome constitution consists of 10 autosomes, with 2n=12, XX in the female and 2n=11, X0 in the male. Meiosis-I shows five bivalents and one univalent for the male worm, and six bivalents for the female worm. T...
journal_title:Tropical biomedicine
pub_type: 杂志文章
doi:
更新日期:2009-12-01 00:00:00
abstract::Methicillin-resistant Staphylococcus aureus (MRSA) is known to cause nosocomial infections and is now becoming an emerging problem in veterinary medicine. The objective of the study was to determine the presence of MRSA in 100 cats and dogs sampled between November 2007 and April 2008 at the University Veterinary Hosp...
journal_title:Tropical biomedicine
pub_type: 杂志文章
doi:
更新日期:2010-12-01 00:00:00
abstract::This is the first report of Synthesiomyia nudiseta (Wulp) (Diptera: Muscidae) on a human corpse discovered in a high-rise building in Malaysia. On 5 March 2008, a decomposing body of an adult female was found on the top floor of a thirteen-story building in Kuala Lumpur, Malaysia. Her body was colonized by S. nu...
journal_title:Tropical biomedicine
pub_type: 杂志文章
doi:
更新日期:2012-03-01 00:00:00
abstract::One hundred and sixty eight rats were trapped from the National Service Training Centres (NSTC) in Kelantan and Terengganu from October 2008 to May 2009. Microscopic agglutination test (MAT) was performed to detect the presence of agglutinating antibodies to Leptospira among the rats caught. All the MAT positive rats ...
journal_title:Tropical biomedicine
pub_type: 杂志文章
doi:
更新日期:2010-04-01 00:00:00