Global scaling down of excitatory postsynaptic responses in cerebellar Purkinje cells impairs developmental synapse elimination.

Abstract:

:Synapse elimination is crucial for precise neural circuit formation during postnatal development. We examined how relative differences in synaptic strengths among competing inputs and/or absolute synaptic strengths contribute to climbing fiber (CF) to Purkinje cell (PC) synapse elimination in the cerebellum. We generated mice with PC-selective deletion of stargazin (TARP γ-2), the major AMPA receptor auxiliary subunit in PCs (γ-2 PC-KO mice). Whereas relative differences between "strong" and "weak" CF-mediated postsynaptic response are preserved, absolute strengths of CF inputs are scaled down globally in PCs of γ-2 PC-KO mice. Although the early phase of CF elimination is normal, dendritic translocation of the strongest CF and the late phase of CF elimination that requires Ca(2+)-dependent activation of Arc/Arg3.1 in PCs are both impaired in γ-2 PC-KO mice. We conclude that, although relative differences in CF synaptic inputs are initially essential, proper synaptic scaling is crucial for accomplishing CF synapse elimination.

journal_name

Cell Rep

journal_title

Cell reports

authors

Kawata S,Miyazaki T,Yamazaki M,Mikuni T,Yamasaki M,Hashimoto K,Watanabe M,Sakimura K,Kano M

doi

10.1016/j.celrep.2014.07.014

subject

Has Abstract

pub_date

2014-08-21 00:00:00

pages

1119-29

issue

4

issn

2211-1247

pii

S2211-1247(14)00581-6

journal_volume

8

pub_type

杂志文章
  • Nucleosome assembly dynamics involve spontaneous fluctuations in the handedness of tetrasomes.

    abstract::DNA wrapping around histone octamers generates nucleosomes, the basic compaction unit of eukaryotic chromatin. Nucleosome stability is carefully tuned to maintain DNA accessibility in transcription, replication, and repair. Using freely orbiting magnetic tweezers, which measure the twist and length of single DNA molec...

    journal_title:Cell reports

    pub_type: 杂志文章

    doi:10.1016/j.celrep.2014.12.022

    authors: Vlijm R,Lee M,Lipfert J,Lusser A,Dekker C,Dekker NH

    更新日期:2015-01-13 00:00:00

  • EBI2 Is Highly Expressed in Multiple Sclerosis Lesions and Promotes Early CNS Migration of Encephalitogenic CD4 T Cells.

    abstract::Arrival of encephalitogenic T cells at inflammatory foci represents a critical step in development of experimental autoimmune encephalomyelitis (EAE), the animal model for multiple sclerosis. EBI2 and its ligand, 7α,25-OHC, direct immune cell localization in secondary lymphoid organs. CH25H and CYP7B1 hydroxylate chol...

    journal_title:Cell reports

    pub_type: 杂志文章

    doi:10.1016/j.celrep.2017.01.020

    authors: Wanke F,Moos S,Croxford AL,Heinen AP,Gräf S,Kalt B,Tischner D,Zhang J,Christen I,Bruttger J,Yogev N,Tang Y,Zayoud M,Israel N,Karram K,Reißig S,Lacher SM,Reichhold C,Mufazalov IA,Ben-Nun A,Kuhlmann T,Wettschureck

    更新日期:2017-01-31 00:00:00

  • APOBEC3A and APOBEC3B Preferentially Deaminate the Lagging Strand Template during DNA Replication.

    abstract::APOBEC family cytidine deaminases have recently been implicated as powerful mutators of cancer genomes. How APOBECs, which are ssDNA-specific enzymes, gain access to chromosomal DNA is unclear. To ascertain the chromosomal ssDNA substrates of the APOBECs, we expressed APOBEC3A and APOBEC3B, the two most probable APOBE...

    journal_title:Cell reports

    pub_type: 杂志文章

    doi:10.1016/j.celrep.2016.01.021

    authors: Hoopes JI,Cortez LM,Mertz TM,Malc EP,Mieczkowski PA,Roberts SA

    更新日期:2016-02-16 00:00:00

  • Transcriptional Signatures of Tau and Amyloid Neuropathology.

    abstract::Alzheimer's disease (AD) is associated with the intracellular aggregation of hyperphosphorylated tau and the accumulation of β-amyloid in the neocortex. We use transgenic mice harboring human tau (rTg4510) and amyloid precursor protein (J20) mutations to investigate transcriptional changes associated with the progress...

    journal_title:Cell reports

    pub_type: 杂志文章

    doi:10.1016/j.celrep.2020.01.063

    authors: Castanho I,Murray TK,Hannon E,Jeffries A,Walker E,Laing E,Baulf H,Harvey J,Bradshaw L,Randall A,Moore K,O'Neill P,Lunnon K,Collier DA,Ahmed Z,O'Neill MJ,Mill J

    更新日期:2020-02-11 00:00:00

  • A Single Let-7 MicroRNA Bypasses LIN28-Mediated Repression.

    abstract::Let-7 microRNAs (miRNAs) are critical regulators of animal development, stem cell differentiation, glucose metabolism, and tumorigenesis. Mammalian genomes contain 12 let-7 isoforms that suppress expression of a common set of target mRNAs. LIN28 proteins selectively block let-7 biogenesis in undifferentiated cells and...

    journal_title:Cell reports

    pub_type: 杂志文章

    doi:10.1016/j.celrep.2015.08.086

    authors: Triboulet R,Pirouz M,Gregory RI

    更新日期:2015-10-13 00:00:00

  • Dual and Direction-Selective Mechanisms of Phosphate Transport by the Vesicular Glutamate Transporter.

    abstract::Vesicular glutamate transporters (VGLUTs) fill synaptic vesicles with glutamate and are thus essential for glutamatergic neurotransmission. However, VGLUTs were originally discovered as members of a transporter subfamily specific for inorganic phosphate (Pi). It is still unclear how VGLUTs accommodate glutamate transp...

    journal_title:Cell reports

    pub_type: 杂志文章

    doi:10.1016/j.celrep.2018.03.055

    authors: Preobraschenski J,Cheret C,Ganzella M,Zander JF,Richter K,Schenck S,Jahn R,Ahnert-Hilger G

    更新日期:2018-04-10 00:00:00

  • The RNA helicase DHX34 activates NMD by promoting a transition from the surveillance to the decay-inducing complex.

    abstract::Nonsense-mediated decay (NMD) is a surveillance mechanism that degrades aberrant mRNAs. A complex comprising SMG1, UPF1, and the translation termination factors eRF1 and eRF3 (SURF) is assembled in the vicinity of a premature termination codon. Subsequently, an interaction with UPF2, UPF3b, and the exon junction compl...

    journal_title:Cell reports

    pub_type: 杂志文章

    doi:10.1016/j.celrep.2014.08.020

    authors: Hug N,Cáceres JF

    更新日期:2014-09-25 00:00:00

  • Identification of a Sgo2-Dependent but Mad2-Independent Pathway Controlling Anaphase Onset in Fission Yeast.

    abstract::The onset of anaphase is triggered by activation of the anaphase-promoting complex/cyclosome (APC/C) following silencing of the spindle assembly checkpoint (SAC). APC/C triggers ubiquitination of Securin and Cyclin B, which leads to loss of sister chromatid cohesion and inactivation of Cyclin B/Cdk1, respectively. Thi...

    journal_title:Cell reports

    pub_type: 杂志文章

    doi:10.1016/j.celrep.2017.01.032

    authors: Meadows JC,Lancaster TC,Buttrick GJ,Sochaj AM,Messin LJ,Del Mar Mora-Santos M,Hardwick KG,Millar JBA

    更新日期:2017-02-07 00:00:00

  • Histone H3 Serine 28 Is Essential for Efficient Polycomb-Mediated Gene Repression in Drosophila.

    abstract::Trimethylation at histone H3K27 is central to the polycomb repression system. Juxtaposed to H3K27 is a widely conserved phosphorylatable serine residue (H3S28) whose function is unclear. To assess the importance of H3S28, we generated a Drosophila H3 histone mutant with a serine-to-alanine mutation at position 28. H3S...

    journal_title:Cell reports

    pub_type: 杂志文章

    doi:10.1016/j.celrep.2015.04.055

    authors: Yung PY,Stuetzer A,Fischle W,Martinez AM,Cavalli G

    更新日期:2015-06-09 00:00:00

  • FACS purification and transcriptome analysis of drosophila neural stem cells reveals a role for Klumpfuss in self-renewal.

    abstract::Drosophila neuroblasts (NBs) have emerged as a model for stem cell biology that is ideal for genetic analysis but is limited by the lack of cell-type-specific gene expression data. Here, we describe a method for isolating large numbers of pure NBs and differentiating neurons that retain both cell-cycle and lineage cha...

    journal_title:Cell reports

    pub_type: 杂志文章

    doi:10.1016/j.celrep.2012.07.008

    authors: Berger C,Harzer H,Burkard TR,Steinmann J,van der Horst S,Laurenson AS,Novatchkova M,Reichert H,Knoblich JA

    更新日期:2012-08-30 00:00:00

  • Enhancement of BLM-DNA2-Mediated Long-Range DNA End Resection by CtIP.

    abstract::DNA double-strand break repair by homologous recombination entails the resection of DNA ends to reveal ssDNA tails, which are used to invade a homologous DNA template. CtIP and its yeast ortholog Sae2 regulate the nuclease activity of MRE11 in the initial stage of resection. Deletion of CtIP in the mouse or SAE2 in ye...

    journal_title:Cell reports

    pub_type: 杂志文章

    doi:10.1016/j.celrep.2017.09.048

    authors: Daley JM,Jimenez-Sainz J,Wang W,Miller AS,Xue X,Nguyen KA,Jensen RB,Sung P

    更新日期:2017-10-10 00:00:00

  • Colonic Lysine Homocysteinylation Induced by High-Fat Diet Suppresses DNA Damage Repair.

    abstract::Colorectal cancer (CRC) onset is profoundly affected by Western diet. Here, we report that high-fat (HF) diet-induced, organ-specific colonic lysine homocysteinylation (K-Hcy) increase might promote CRC onset by impeding DNA damage repair. HF chow induced elevated methionyl-tRNA synthetase (MARS) expression and K-Hcy ...

    journal_title:Cell reports

    pub_type: 杂志文章

    doi:10.1016/j.celrep.2018.09.022

    authors: Wang D,Zhao R,Qu YY,Mei XY,Zhang X,Zhou Q,Li Y,Yang SB,Zuo ZG,Chen YM,Lin Y,Xu W,Chen C,Zhao SM,Zhao JY

    更新日期:2018-10-09 00:00:00

  • CTCF Recruits Centromeric Protein CENP-E to the Pericentromeric/Centromeric Regions of Chromosomes through Unusual CTCF-Binding Sites.

    abstract::The role of CTCF in stabilizing long-range interactions between chromatin sites essential for maintaining nuclear architecture is well established. Most of these interactions involve recruitment of the cohesin complex to chromatin via CTCF. We find that CTCF also interacts with the centromeric protein CENP-E both in v...

    journal_title:Cell reports

    pub_type: 杂志文章

    doi:10.1016/j.celrep.2015.08.005

    authors: Xiao T,Wongtrakoongate P,Trainor C,Felsenfeld G

    更新日期:2015-09-08 00:00:00

  • Combinatorial Smad2/3 Activities Downstream of Nodal Signaling Maintain Embryonic/Extra-Embryonic Cell Identities during Lineage Priming.

    abstract::Epiblast cells in the early post-implantation stage mammalian embryo undergo a transition described as lineage priming before cell fate allocation, but signaling pathways acting upstream remain ill defined. Genetic studies demonstrate that Smad2/3 double-mutant mouse embryos die shortly after implantation. To learn mo...

    journal_title:Cell reports

    pub_type: 杂志文章

    doi:10.1016/j.celrep.2018.07.077

    authors: Senft AD,Costello I,King HW,Mould AW,Bikoff EK,Robertson EJ

    更新日期:2018-08-21 00:00:00

  • Crystal Structure and Activity of the Endoribonuclease Domain of the piRNA Pathway Factor Maelstrom.

    abstract::PIWI-interacting RNAs (piRNAs) protect the genome from transposons in animal gonads. Maelstrom (Mael) is an evolutionarily conserved protein, composed of a high-mobility group (HMG) domain and a MAEL domain, and is essential for piRNA-mediated transcriptional transposon silencing in various species, such as Drosophila...

    journal_title:Cell reports

    pub_type: 杂志文章

    doi:10.1016/j.celrep.2015.03.030

    authors: Matsumoto N,Sato K,Nishimasu H,Namba Y,Miyakubi K,Dohmae N,Ishitani R,Siomi H,Siomi MC,Nureki O

    更新日期:2015-04-21 00:00:00

  • Principles of Membrane Adaptation Revealed through Environmentally Induced Bacterial Lipidome Remodeling.

    abstract::Cells, from microbes to mammals, adapt their membrane lipid composition in response to environmental changes to maintain optimal properties. Global patterns of lipidome remodeling are poorly understood, particularly in organisms with simple lipid compositions that can provide insight into fundamental principles of mem...

    journal_title:Cell reports

    pub_type: 杂志文章

    doi:10.1016/j.celrep.2020.108165

    authors: Chwastek G,Surma MA,Rizk S,Grosser D,Lavrynenko O,Rucińska M,Jambor H,Sáenz J

    更新日期:2020-09-22 00:00:00

  • The Olfactory Logic behind Fruit Odor Preferences in Larval and Adult Drosophila.

    abstract::Despite the comprehensive knowledge on odor coding, our understanding of the relationship between sensory input and behavioral output in Drosophila remains weak. Here, we measure the behavioral responses generated by larval and adult flies in response to 34 fruit odors and find that larval preference for fruit odors d...

    journal_title:Cell reports

    pub_type: 杂志文章

    doi:10.1016/j.celrep.2018.04.085

    authors: Dweck HKM,Ebrahim SAM,Retzke T,Grabe V,Weißflog J,Svatoš A,Hansson BS,Knaden M

    更新日期:2018-05-22 00:00:00

  • Sub-minute Phosphoregulation of Cell Cycle Systems during Plasmodium Gamete Formation.

    abstract::The transmission of malaria parasites to mosquitoes relies on the rapid induction of sexual reproduction upon their ingestion into a blood meal. Haploid female and male gametocytes become activated and emerge from their host cells, and the males enter the cell cycle to produce eight microgametes. The synchronized natu...

    journal_title:Cell reports

    pub_type: 杂志文章

    doi:10.1016/j.celrep.2017.10.071

    authors: Invergo BM,Brochet M,Yu L,Choudhary J,Beltrao P,Billker O

    更新日期:2017-11-14 00:00:00

  • Human-chromatin-related protein interactions identify a demethylase complex required for chromosome segregation.

    abstract::Chromatin regulation is driven by multicomponent protein complexes, which form functional modules. Deciphering the components of these modules and their interactions is central to understanding the molecular pathways these proteins are regulating, their functions, and their relation to both normal development and dise...

    journal_title:Cell reports

    pub_type: 杂志文章

    doi:10.1016/j.celrep.2014.05.050

    authors: Marcon E,Ni Z,Pu S,Turinsky AL,Trimble SS,Olsen JB,Silverman-Gavrila R,Silverman-Gavrila L,Phanse S,Guo H,Zhong G,Guo X,Young P,Bailey S,Roudeva D,Zhao D,Hewel J,Li J,Gräslund S,Paduch M,Kossiakoff AA,Lupien M,

    更新日期:2014-07-10 00:00:00

  • Hunger Potentiates the Habenular Winner Pathway for Social Conflict by Orexin-Promoted Biased Alternative Splicing of the AMPA Receptor Gene.

    abstract::Many animals fight for dominance between conspecifics. Because winners could obtain more resources than losers, fighting outcomes are important for the animal's survival, especially in a situation with insufficient resources, such as hunger. However, it remains unclear whether and how hunger affects fighting outcomes....

    journal_title:Cell reports

    pub_type: 杂志文章

    doi:10.1016/j.celrep.2020.107790

    authors: Nakajo H,Chou MY,Kinoshita M,Appelbaum L,Shimazaki H,Tsuboi T,Okamoto H

    更新日期:2020-06-23 00:00:00

  • An Allosteric Interaction Network Promotes Conformation State-Dependent Eviction of the Nas6 Assembly Chaperone from Nascent 26S Proteasomes.

    abstract::The 26S proteasome is the central ATP-dependent protease in eukaryotes and is essential for organismal health. Proteasome assembly is mediated by several dedicated, evolutionarily conserved chaperone proteins. These chaperones associate transiently with assembly intermediates but are absent from mature proteasomes. Ch...

    journal_title:Cell reports

    pub_type: 杂志文章

    doi:10.1016/j.celrep.2018.12.042

    authors: Nemec AA,Peterson AK,Warnock JL,Reed RG,Tomko RJ Jr

    更新日期:2019-01-08 00:00:00

  • Molecular Basis of Zinc-Dependent Endocytosis of Human ZIP4 Transceptor.

    abstract::Nutrient transporters can be rapidly removed from the cell surface via substrate-stimulated endocytosis as a way to control nutrient influx, but the molecular underpinnings are not well understood. In this work, we focus on zinc-dependent endocytosis of human ZIP4 (hZIP4), a zinc transporter that is essential for diet...

    journal_title:Cell reports

    pub_type: 杂志文章

    doi:10.1016/j.celrep.2020.107582

    authors: Zhang C,Sui D,Zhang T,Hu J

    更新日期:2020-04-28 00:00:00

  • HIV-1 adaptation to antigen processing results in population-level immune evasion and affects subtype diversification.

    abstract::The recent HIV-1 vaccine failures highlight the need to better understand virus-host interactions. One key question is why CD8(+) T cell responses to two HIV-Gag regions are uniquely associated with delayed disease progression only in patients expressing a few rare HLA class I variants when these regions encode epitop...

    journal_title:Cell reports

    pub_type: 杂志文章

    doi:10.1016/j.celrep.2014.03.031

    authors: Tenzer S,Crawford H,Pymm P,Gifford R,Sreenu VB,Weimershaus M,de Oliveira T,Burgevin A,Gerstoft J,Akkad N,Lunn D,Fugger L,Bell J,Schild H,van Endert P,Iversen AKN

    更新日期:2014-04-24 00:00:00

  • Structural Determination of the Broadly Reactive Anti-IGHV1-69 Anti-idiotypic Antibody G6 and Its Idiotope.

    abstract::The heavy chain IGHV1-69 germline gene exhibits a high level of polymorphism and shows biased use in protective antibody (Ab) responses to infections and vaccines. It is also highly expressed in several B cell malignancies and autoimmune diseases. G6 is an anti-idiotypic monoclonal Ab that selectively binds to IGHV1-6...

    journal_title:Cell reports

    pub_type: 杂志文章

    doi:10.1016/j.celrep.2017.11.056

    authors: Avnir Y,Prachanronarong KL,Zhang Z,Hou S,Peterson EC,Sui J,Zayed H,Kurella VB,McGuire AT,Stamatatos L,Hilbert BJ,Bohn MF,Kowalik TF,Jensen JD,Finberg RW,Wang JP,Goodall M,Jefferis R,Zhu Q,Kurt Yilmaz N,Schiffer CA

    更新日期:2017-12-12 00:00:00

  • Measuring Integrin Conformational Change on the Cell Surface with Super-Resolution Microscopy.

    abstract::We use super-resolution interferometric photoactivation and localization microscopy (iPALM) and a constrained photoactivatable fluorescent protein integrin fusion to measure the displacement of the head of integrin lymphocyte function-associated 1 (LFA-1) resulting from integrin conformational change on the cell surfa...

    journal_title:Cell reports

    pub_type: 杂志文章

    doi:10.1016/j.celrep.2018.01.062

    authors: Moore TI,Aaron J,Chew TL,Springer TA

    更新日期:2018-02-13 00:00:00

  • Signaling Networks among Stem Cell Precursors, Transit-Amplifying Progenitors, and their Niche in Developing Hair Follicles.

    abstract::The hair follicle (HF) is a complex miniorgan that serves as an ideal model system to study stem cell (SC) interactions with the niche during growth and regeneration. Dermal papilla (DP) cells are required for SC activation during the adult hair cycle, but signal exchange between niche and SC precursors/transit-amplif...

    journal_title:Cell reports

    pub_type: 杂志文章

    doi:10.1016/j.celrep.2016.02.078

    authors: Rezza A,Wang Z,Sennett R,Qiao W,Wang D,Heitman N,Mok KW,Clavel C,Yi R,Zandstra P,Ma'ayan A,Rendl M

    更新日期:2016-03-29 00:00:00

  • HoxBlinc RNA Recruits Set1/MLL Complexes to Activate Hox Gene Expression Patterns and Mesoderm Lineage Development.

    abstract::Trithorax proteins and long-intergenic noncoding RNAs are critical regulators of embryonic stem cell pluripotency; however, how they cooperatively regulate germ layer mesoderm specification remains elusive. We report here that HoxBlinc RNA first specifies Flk1(+) mesoderm and then promotes hematopoietic differentiatio...

    journal_title:Cell reports

    pub_type: 杂志文章

    doi:10.1016/j.celrep.2015.12.007

    authors: Deng C,Li Y,Zhou L,Cho J,Patel B,Terada N,Li Y,Bungert J,Qiu Y,Huang S

    更新日期:2016-01-05 00:00:00

  • KDM4C and ATF4 Cooperate in Transcriptional Control of Amino Acid Metabolism.

    abstract::The histone lysine demethylase KDM4C is often overexpressed in cancers primarily through gene amplification. The molecular mechanisms of KDM4C action in tumorigenesis are not well defined. Here, we report that KDM4C transcriptionally activates amino acid biosynthesis and transport, leading to a significant increase in...

    journal_title:Cell reports

    pub_type: 杂志文章

    doi:10.1016/j.celrep.2015.12.053

    authors: Zhao E,Ding J,Xia Y,Liu M,Ye B,Choi JH,Yan C,Dong Z,Huang S,Zha Y,Yang L,Cui H,Ding HF

    更新日期:2016-01-26 00:00:00

  • Phospho-Ser784-VCP Is Required for DNA Damage Response and Is Associated with Poor Prognosis of Chemotherapy-Treated Breast Cancer.

    abstract::Spatiotemporal protein reorganization at DNA damage sites induced by genotoxic chemotherapies is crucial for DNA damage response (DDR), which influences treatment response by directing cancer cell fate. This process is orchestrated by valosin-containing protein (VCP), an AAA+ ATPase that extracts polyubiquinated chrom...

    journal_title:Cell reports

    pub_type: 杂志文章

    doi:10.1016/j.celrep.2020.107745

    authors: Zhu C,Rogers A,Asleh K,Won J,Gao D,Leung S,Li S,Vij KR,Zhu J,Held JM,You Z,Nielsen TO,Shao J

    更新日期:2020-06-09 00:00:00

  • Lipidomic Adaptations in White and Brown Adipose Tissue in Response to Exercise Demonstrate Molecular Species-Specific Remodeling.

    abstract::Exercise improves whole-body metabolic health through adaptations to various tissues, including adipose tissue, but the effects of exercise training on the lipidome of white adipose tissue (WAT) and brown adipose tissue (BAT) are unknown. Here, we utilize MS/MSALL shotgun lipidomics to determine the molecular signatur...

    journal_title:Cell reports

    pub_type: 杂志文章

    doi:10.1016/j.celrep.2017.01.038

    authors: May FJ,Baer LA,Lehnig AC,So K,Chen EY,Gao F,Narain NR,Gushchina L,Rose A,Doseff AI,Kiebish MA,Goodyear LJ,Stanford KI

    更新日期:2017-02-07 00:00:00