Abstract:
:Synapse elimination is crucial for precise neural circuit formation during postnatal development. We examined how relative differences in synaptic strengths among competing inputs and/or absolute synaptic strengths contribute to climbing fiber (CF) to Purkinje cell (PC) synapse elimination in the cerebellum. We generated mice with PC-selective deletion of stargazin (TARP γ-2), the major AMPA receptor auxiliary subunit in PCs (γ-2 PC-KO mice). Whereas relative differences between "strong" and "weak" CF-mediated postsynaptic response are preserved, absolute strengths of CF inputs are scaled down globally in PCs of γ-2 PC-KO mice. Although the early phase of CF elimination is normal, dendritic translocation of the strongest CF and the late phase of CF elimination that requires Ca(2+)-dependent activation of Arc/Arg3.1 in PCs are both impaired in γ-2 PC-KO mice. We conclude that, although relative differences in CF synaptic inputs are initially essential, proper synaptic scaling is crucial for accomplishing CF synapse elimination.
journal_name
Cell Repjournal_title
Cell reportsauthors
Kawata S,Miyazaki T,Yamazaki M,Mikuni T,Yamasaki M,Hashimoto K,Watanabe M,Sakimura K,Kano Mdoi
10.1016/j.celrep.2014.07.014subject
Has Abstractpub_date
2014-08-21 00:00:00pages
1119-29issue
4issn
2211-1247pii
S2211-1247(14)00581-6journal_volume
8pub_type
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