Characteristics of evodiamine-exerted stimulatory effects on rat jejunal contractility.

Abstract:

:This study was designed to characterise the effects of evodiamine on intestinal contractility and reveal the correlated mechanisms. Evodiamine (2.5-80.0 μM) increased normal jejunal contractility and jejunal hypocontractility established under a variety of experimental conditions. Evodiamine-exerted stimulatory effects were blocked by the L-type Ca(2+) channel blocker nifedipine or abolished in the Ca(2+)-free assay condition. The stimulatory effects of evodiamine on jejunal contractility were partially blocked in the presence of neurotoxin tetrodotoxin or endogenous acetylcholine synthesis blocker hemicholinium-3 or muscarinic receptor antagonist atropine, respectively. Evodiamine-exerted stimulatory effects were blocked by c-kit receptor tyrosine kinase inhibitor imatinib. Evodiamine increased myosin phosphorylation in jejunal smooth muscle of constipation-prominent rats. These results showed that evodiamine-exerted stimulatory effects on jejunal segments are Ca(2+)-dependent, need the presence of interstitial cell of Cajal, requirement of cholinergic neuron and correlate with increased myosin phosphorylation, implicating the potential value of evodiamine in relieving hypo-motility disorders.

journal_name

Nat Prod Res

journal_title

Natural product research

authors

Xiong YJ,Chen DP,Peng JY,Wang JY,Lv BC,Liu FF,Lin Y

doi

10.1080/14786419.2014.947485

subject

Has Abstract

pub_date

2015-01-01 00:00:00

pages

388-91

issue

4

eissn

1478-6419

issn

1478-6427

journal_volume

29

pub_type

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