Abstract:
AIM:To investigate the impact of three irrigation protocols, activated by three different methods, on mature biofilms of Enterococcus faecalis in vitro. METHODOLOGY:Root canals in 280 single-rooted teeth were instrumented using a rotary Ni-Ti system. Biofilms of E. faecalis were generated based on a previously established protocol. Samples were randomly divided into three experimental (n = 80) and one control (n = 40) group based on the irrigation protocol employed: group 1 (NaOCl + Etidronic acid), 1 : 1 mixture of 6% NaOCl and 18% etidronic acid; group 2 (NaOCl-EDTA), 3% NaOCl followed by 17% EDTA; group 3 (NaOCl-EDTA-NaOCl), 3% NaOCl followed by 17% EDTA and a final flush of 3% NaOCl. Saline served as the control. Samples were further divided into four subgroups (n = 20) based on the activation method: subgroup A, no activation; subgroup B, ultrasonic activation; group C, diode laser; group D, Er:YAG laser. Confocal laser scanning microscopy was used to assess bacterial viability in situ. Root dentine powder was obtained for determining the colony-forming units (CFU mL(-1) ). Data were analysed by appropriate statistical analyses with P = 0.05. RESULTS:All experimental irrigation protocols caused complete destruction of the biofilm in the root canal lumen. Within the dentinal tubules, all groups had a significantly higher percentage of dead bacteria than the saline control (P < 0.05). There was no significant difference between NaOCl + etidronic acid and NaOCl-EDTA-NaOCl (P > 0.05), whereas both groups brought about more bacterial reduction than NaOCl-EDTA (P < 0.05). There was no significant difference between diode laser and Er:YAG laser in any of the groups (P > 0.05). Both diode and Er:YAG laser were more effective than ultrasonic activation and conventional syringe irrigation in reducing E. fecalis biofilms (P < 0.05). CONCLUSIONS:The use of NaOCl after or in combination with a chelator caused the greatest reduction of E. faecalis. Diode laser and Er:YAG laser activation were superior to ultrasonics in dentinal tubule disinfection.
journal_name
Int Endod Jjournal_title
International endodontic journalauthors
Neelakantan P,Cheng CQ,Mohanraj R,Sriraman P,Subbarao C,Sharma Sdoi
10.1111/iej.12354subject
Has Abstractpub_date
2015-06-01 00:00:00pages
602-10issue
6eissn
0143-2885issn
1365-2591journal_volume
48pub_type
杂志文章abstract:AIM:To compare the cyclic fatigue resistance of ProTaper Next files (PTN; Dentsply Maillefer, Ballaigues, Switzerland) with Twisted Files (TF; SybronEndo, Orange, CA, USA), HyFlex CM (HF; ColténeEndo/Whaledent, Inc, Cuyahoga Falls, OH, USA) and ProTaper Universal (PT; Dentsply Maillefer). METHODOLOGY:Size 25, .06 tape...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12244
更新日期:2014-11-01 00:00:00
abstract::Animal testing is crucial in situations when research on humans is not allowed because of unknown health risks and ethical concerns. The current project aims to develop reporting guidelines exclusively for animal studies in Endodontology, using an established consensus-based methodology. The guidelines have been named...
journal_title:International endodontic journal
pub_type: 杂志文章,评审
doi:10.1111/iej.13477
更新日期:2021-01-15 00:00:00
abstract:AIM:To evaluate ex vivo the long-term sealing ability of the SE Resilon Epiphany system as an apical root-end filling material. METHODOLOGY:A total of 60 standardized horizontal bovine root sections were divided into three groups filled with either gutta-percha with AH 26, tooth-coloured mineral trioxide aggregate (MT...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2009.01583.x
更新日期:2009-10-01 00:00:00
abstract::Models which simulate oral conditions pertaining to endodontic surgical procedures are most suitable if they contain natural teeth and have hard and soft tissues which handle realistically. In addition, the model should possess radiological characteristics similar to bone. A simple custom-built model which fulfils the...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:
更新日期:1993-05-01 00:00:00
abstract:AIM:To evaluate the cytotoxicity and the mechanism of cell aggression of peracetic acid (PA) in comparison with sodium hypochlorite (NaOCl). METHODOLOGY:L929 fibroblasts were exposed to 1% PA and 2.5% NaOCl, at several dilutions for 10 min. The following parameters were evaluated: cell metabolism by methylthiazol tetr...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12750
更新日期:2018-05-01 00:00:00
abstract:AIM:To assess the inflammatory potential of biofilm matrix constituents of Enterococcus faecalis and Pseudomonas aeruginosa monospecies biofilms on macrophages. METHODOLOGY:In vitro biofilms of E. faecalis and P. aeruginosa were grown (7 days) in aerobic and anaerobic conditions. The biofilm matrix components: exopoly...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13093
更新日期:2019-07-01 00:00:00
abstract:AIM:This study aimed to examine the fracture resistance of teeth restored with cast post and partial cores supported by different heights of coronal tooth structure. METHODOLOGY:Four specimen groups were prepared. Group A was the control and consisted of 10 single-rooted teeth restored with post and cores cemented in ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.2002.00596.x
更新日期:2002-11-01 00:00:00
abstract:AIM:To compare torque and angle of rotation at fracture of new and used K3 rotary instruments. The relation between size of instrument and torque at fracture was also investigated. METHODOLOGY:Nickel-titanium (Ni-Ti).06 K3 rotary instruments were used in a crown-down manner at 300 r.p.m. to prepare canals in resin blo...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.2003.00732.x
更新日期:2003-11-01 00:00:00
abstract:AIM:To analyse torque and force generated whilst shaping curved canals using rotary instruments. METHODOLOGY:A specially designed computer-controlled testing platform was used to record events during the shaping of straight and curved canals in plastic blocks and in extracted human teeth using ProFile.04 instruments. ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.0143-2885.2001.00494.x
更新日期:2002-04-01 00:00:00
abstract::The purpose of this investigation was to study the effects of a Nd:YAG laser on the cut surface of teeth using scanning electron microscopy (SEM). Eighteen single-rooted teeth were cleaned, shaped, and obturated with gutta-percha and root canal sealer. The apical 3 mm of each tooth were resected with a diamond fissure...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1992.tb00758.x
更新日期:1992-11-01 00:00:00
abstract:AIM:To assay the toxicity of the single-methacrylate-based sealer urethane dimethacrylate (UDMA) (EndoRez) in terms of cell growth and pro-inflammatory cytokines release, in expanded ex vivo human dental pulp stem cells (hDPSCs), human periodontal ligament stem cells (hPDLSCs), human gingival fibroblasts (hGFs) and hum...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12230
更新日期:2014-09-01 00:00:00
abstract::Four hundred and fifty-five extracted mandibular incisor and 340 extracted mandibular premolar teeth were radiographed to assess the incidence of twin canals as visualized on radiographs taken in the mesio-distal direction. The ability to detect the presence of these twin canals by viewing radiographs taken in the sta...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1991.tb00808.x
更新日期:1991-03-01 00:00:00
abstract:AIM:To evaluate the influence of delayed scanning on images obtained with two PSPs digital systems and on the diagnostic accuracy of vertical root fracture (VRF) by means of objective and subjective analyses. METHODOLOGY:Forty single-rooted human teeth were divided into two groups, one without VRFs and another with VR...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12547
更新日期:2016-10-01 00:00:00
abstract:AIM:To evaluate ex vivo the accuracy of two electronic apex locators during root canal length determination in primary incisor and molar teeth with different stages of physiological root resorption. METHODOLOGY:One calibrated examiner determined the root canal length in 17 primary incisors and 16 primary molars (total...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2007.01366.x
更新日期:2008-04-01 00:00:00
abstract::The large variations observed in dentists' management of periapical lesions in endodontically treated teeth suggest that disease concepts used in clinical practice should be investigated. In the present study it was hypothesized that dentists regard various periapical conditions as different stages on a health continu...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1994.tb00270.x
更新日期:1994-11-01 00:00:00
abstract:AIM:To analyse responses from dental practitioners (DPs) on how secure they felt as a newly graduated dentist, level of confidence or self-efficacy when performing root canal treatment (RCT), and if undergraduate (UG) education in Endodontics adequately met their needs in a dental practice. METHODOLOGY:An electronic q...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13455
更新日期:2020-11-30 00:00:00
abstract::The antibacterial activity of four elements (zinc, copper, fluoride and mercury), previously reported to be released from dental restorative materials, was investigated. This was carried out using two aerobic micro-organisms isolated from dentine beneath cavities in the ferret. These were grown in the presence of the ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1989.tb00499.x
更新日期:1989-01-01 00:00:00
abstract:AIM:To compare the efficacy of reciprocating and rotary techniques with that of hand files for removing gutta-percha and sealer from root canals. METHODOLOGY:The root canals of fifty-four human extracted maxillary central incisors were cleaned and shaped using a crown-down technique to a size 40 and filled with gutta-...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12085
更新日期:2013-10-01 00:00:00
abstract:AIM:The aim of this study was to determine the ability of the Quantec Series 2000 rotary nickel-titanium endodontic system to remove dentinal debris and smear layer produced during canal preparation. METHODOLOGY:A first group (control) of 12 curved root canals was prepared using conventional manual instruments and the...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.1999.00231.x
更新日期:1999-05-01 00:00:00
abstract::Root-canal-filling materials are either placed directly onto vital periapical tissues or may leach through dentine. The tissue response to these materials therefore becomes important and may influence the outcome of endodontic treatment. This paper is a review of the biocompatibility of contemporary orthograde and ret...
journal_title:International endodontic journal
pub_type: 杂志文章,评审
doi:10.1046/j.1365-2591.2003.00637.x
更新日期:2003-03-01 00:00:00
abstract:AIM:To determine whether chemokines such as SDF-1 and monocyte chemoattractant protein-1 (MCP-1) are responsible for hydrogen peroxide (H2 O2 )-induced extracellular matrix (ECM) degradation and to identify the underlying mechanism in human dental pulp cells (HDPCs). METHOD:Human dental pulp cells were exposed to 0.4 ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12147
更新日期:2014-03-01 00:00:00
abstract::The aim of the present systematic review was to evaluate the effectiveness of technology-enhanced learning (TEL) in the field of Endodontics to improve educational outcomes compared to traditional learning methods. Randomized controlled studies published in English were identified from two electronic databases (PubMed...
journal_title:International endodontic journal
pub_type: 杂志文章,meta分析
doi:10.1111/iej.12995
更新日期:2019-02-01 00:00:00
abstract:AIMS:To investigate and compare views on management of deep caries and the exposed pulp by Endodontic Society members in Ireland (Irish Endodontic Society [IES]) and Italy (Accademia Italiana di Endodonzia [AIE]). Further aims were to investigate the influence of patient-related factors (age, symptoms) and operator-rel...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13418
更新日期:2020-09-24 00:00:00
abstract:AIM:The aim of this study was to compare two protocols to examine bacterial colonization in teeth associated with chronic apical periodontitis with acute episodes (ap), using light microscopy (LM), transmission electron microscopy (TEM) and scanning electron microscopy (SEM). METHODOLOGY:Nine root samples (seven teeth...
journal_title:International endodontic journal
pub_type: 杂志文章,随机对照试验
doi:10.1111/j.1365-2591.2009.01594.x
更新日期:2009-10-01 00:00:00
abstract:AIM:To examine the effects of calcium hydroxide (Ca(OH)2), transforming growth factor-beta (TGF-beta 1), and Ca(OH)2/TGF-beta 1 coadministration on TGF-beta 1 and interleukin-6 (IL-6) synthesis by early (subculture 1) and late (subculture 5) osteoblast cultures. METHODOLOGY:Early and late cultures were established usi...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.2000.00326.x
更新日期:2000-09-01 00:00:00
abstract:AIM:This study evaluated the effects of different root canal sealers on the bond strength of a fibreglass post cemented with self-adhesive resin cements. METHODOLOGY:The root canals of 50 extracted maxillary single-rooted canine teeth were prepared with the crown-down technique and randomly divided into five groups ac...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2010.01831.x
更新日期:2011-04-01 00:00:00
abstract:AIM:To assess if a contrast medium improved diagnostic yield of endodontic radiographs. METHODOLOGY:Forty-five extracted mandibular premolar teeth were radiographed in bucco-lingual and mesio-distal planes. Access cavities were prepared, pulpal tissue extirpated and Ultravist contrast medium introduced under pressure....
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2004.00872.x
更新日期:2004-12-01 00:00:00
abstract:AIMS:The aims of this study were to investigate the pattern of transmission of laser light through carious human molar teeth, and to examine the effect on light transmission of reactions to caries in the dentine. METHODOLOGY:Sectioned carious human molars were examined whilst illuminated from their external surfaces b...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.2001.00428.x
更新日期:2001-10-01 00:00:00
abstract:AIM:To evaluate whether the type of display device affects the detection of vertical root fractures (VRFs) on digital radiographs in unfilled canals and canals with fibreglass posts. METHODOLOGY:Forty single-rooted human teeth were decoronated, and the root canals were prepared. The teeth were divided into 2 groups: c...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12466
更新日期:2016-04-01 00:00:00
abstract:AIM:To evaluate the torsional properties of the NiTi glide path reciprocating instruments R-Pilot (VDW, Munich, Germany) and WaveOne Gold Glider (Dentsply Sirona, Ballaigues, Switzerland). METHODOLOGY:Ten R-Pilot (size 12.5, .04 taper) and 10 WaveOne Gold Glider (size 15, .02v taper) instruments were used. The torque ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13068
更新日期:2019-06-01 00:00:00