Abstract：
:Ebselen is a seleno-organic compound that causes cell death in several cancer cell types. The mechanisms underlying its deleterious effects have not been fully elucidated. In this study, the effects of ebselen (1 μM-40 μM) on AR42J tumor cells have been examined. Cell viability was studied using AlamarBlue(®) test. Cell cycle phase determination was carried out by flow cytometry. Changes in intracellular free Ca(2+) concentration were followed by fluorimetry analysis of fura-2-loaded cells. Distribution of mitochondria, mitochondrial Ca(2+) concentration and mitochondrial membrane potential were monitored by confocal microscopy of cells loaded with Mitotracker Green™ FM, rhod-2 or TMRM respectively. Caspase-3 activity was calculated following the luorogenic substrate ACDEVD-AMC signal with a spectrofluorimeter. Results show that cell viability decreased in the presence of ebselen. An increase in the number of cells in the S-phase of the cell cycle was observed. Ebselen induced a concentration-dependent mobilization of Ca(2+) from agonist- and thapsigargin-sensitive Ca(2+) pools. Ebselen induced also a transient increase in mitochondrial Ca(2+) concentration, a progressive decrease of the mitochondrial membrane potential and a disruption of the mitochondrial network. Finally, a concentration-dependent increase in caspase-3 activity was detected. We conclude that ebselen exerts deleterious actions on the cells that involve the impairment of mitochondrial physiology and the activation of caspase-3-mediated apoptotic pathway.
journal_name
Toxicol Lettjournal_title
Toxicology lettersauthors
Santofimia-Castaño P,Garcia-Sanchez L,Ruy DC,Fernandez-Bermejo M,Salido GM,Gonzalez Adoi
10.1016/j.toxlet.2014.07.025subject
Has Abstractpub_date
2014-09-17 00:00:00pages
465-73issue
3eissn
0378-4274issn
1879-3169pii
S0378-4274(14)01198-9journal_volume
229pub_type
杂志文章abstract：:Exposure to chemicals released during urban waste disposal and treatment is increasingly regarded as a potential occupational health issue. Indeed, several toxic metals emitted by an incinerator, including As, Be, Cd, Cr, Pb, Mn, Hg, Ni and V, have potentially toxic properties and their exposure, therefore, may be of ...
journal_title：Toxicology letters
pub_type： 杂志文章
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更新日期：2017-04-15 00:00:00
abstract：:Molecular epidemiologic research involves the identification of relations between previous exposure to some putative causative agent and subsequent biological effects in a cluster of individuals in populations. There is intensive current research in the field of molecular epidemiology, and this research has a direct i...
journal_title：Toxicology letters
pub_type： 杂志文章,评审
doi：10.1016/0378-4274(95)03594-x
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abstract：:Isolated rat hepatocytes incubated aerobically formed measurable amounts of ethane, a parameter for lipid peroxidation. This ethane formation increased several-fold due to carbon tetrachloride (CCl4) depending on its concentration. Cell damage as measured by trypan blue uptake and lactate dehydrogenase release poorly ...
journal_title：Toxicology letters
pub_type： 杂志文章
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更新日期：1981-06-01 00:00:00
abstract：:In this work the in vitro reactions of phosphine with intact red blood cells and membrane-free hemoglobin extracts are reported. We demonstrate that phosphine or phosphine derivatives induce dense aggregates of denatured hemoglobin known as 'Heinz bodies' in intact red blood cells. The reaction products include irreve...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/0378-4274(91)90117-o
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journal_title：Toxicology letters
pub_type： 杂志文章
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更新日期：1995-01-01 00:00:00
abstract：:O,O,S-Trimethylphosphorothioate (OOS-Me) and O,S,S-trimethylphosphorodithioate (OSS-Me) are impurities in technical grade malathion and related insecticides which have been shown to cause delayed death in rats following a single oral dose of 40-60 mg/kg. In connection with studies on the mode of action of these compou...
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pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Toxicology letters
pub_type： 杂志文章
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更新日期：2000-04-10 00:00:00
abstract：:Cadmium (Cd), is a well-known environmental and occupational hazard with a potent neurotoxic action. However, the mechanism underlying cadmium-induced neurotoxicity remains unclear. Herein, we exposed Neuro-2a cells to different concentrations of cadmium chloride (CdCl2) (12.5, 25 and 50 μM) for 24 h and found that Cd...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2018.07.015
更新日期：2018-10-01 00:00:00
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journal_title：Toxicology letters
pub_type： 杂志文章
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更新日期：2015-10-01 00:00:00
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journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/s0378-4274(98)00095-2
更新日期：1998-08-01 00:00:00
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journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2004.04.035
更新日期：2004-09-25 00:00:00
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journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2012.04.014
更新日期：2012-06-20 00:00:00
abstract：:Traditional gold mining, using metallic mercury (Hg(0)) to form gold amalgam, followed by burning to remove the Hg(0), is widely used in South America, Africa and Asia. The gold is sold to merchants who burn it again to eliminate remaining Hg(0). In Ecuador, 200 gold miners, 37 gold merchants and 72 referents were stu...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2011.09.006
更新日期：2012-08-13 00:00:00
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journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/0378-4274(89)90073-8
更新日期：1989-05-01 00:00:00
abstract：:The metabolic activity of pre-implantation blastocysts recovered from delayed mice before and after administration of lead was estimated by measuring their oxygen consumption using a spectrophotometer technique. Normal blastocysts, activated for implantation, increased their oxygen consumption by about 50% compared wi...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/0378-4274(91)90163-z
更新日期：1991-05-01 00:00:00
abstract：:Benzene is a common occupational hazard and a ubiquitous environmental pollutant. Benzene exposure at the levels even below 1ppm still showed hematotoxicity. It is widely accepted that the metabolites of benzene play important roles in the benzene toxicity to the hematopoietic system, but little is known about the eff...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2011.03.012
更新日期：2011-06-24 00:00:00
abstract：:The common food preparation processes, frying, broiling and baking, can give rise to air pollutants that are known to be mutagenic and carcinogenic in animal tests. A large number of persons can be exposed to such fumes as cooking is performed in most households and in many commercial enterprises. Additional studies o...
journal_title：Toxicology letters
pub_type： 杂志文章,评审
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更新日期：1994-06-01 00:00:00
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journal_title：Toxicology letters
pub_type： 杂志文章
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更新日期：2001-01-03 00:00:00
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journal_title：Toxicology letters
pub_type： 杂志文章
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journal_title：Toxicology letters
pub_type： 杂志文章
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abstract：:Effects of intestinal damage on thyroid carcinogenesis due to amitrole (AT) were examined in F344 male rats initiated with N-bis(2-hydroxypropyl)nitrosamine (DHPN). In experiment 1, rats were provided with diet containing 0.03% AT for 20 weeks after a single subcutaneous injection of DHPN (2800 mg/kg body weight), and...
journal_title：Toxicology letters
pub_type： 杂志文章
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更新日期：2006-06-20 00:00:00
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journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2011.04.020
更新日期：2011-08-10 00:00:00
abstract：:Hyperbilirubinemia is a common clinical phenomenon observed in human newborns. A high level of bilirubin can result in severe jaundice and bilirubin encephalopathy. However, the cellular mechanisms underlying bilirubin excitotoxicity are unclear. Our previous studies showed the action of gamma-aminobutyric acid (GABA)...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2015.10.008
更新日期：2016-01-05 00:00:00
abstract：:Fumonisin B1 (FB1) and beauvericin (BEA) are secondary metabolites of filamentous fungi, which under appropriate temperature and humidity conditions may develop on various foods and feeds. To date few studies have been performed to evaluate the toxicological and endocrine disrupting effects of FB1 and BEA. The present...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2016.05.021
更新日期：2016-08-22 00:00:00
abstract：:A single intraperitoneal injection of cadmium chloride (CdCl2, 0.32 mg/kg body weight) was administered to 15 adult female palm squirrels (Funambulus pennanti (Wroughton] and the hypothalamic 5-hydroxytryptamine (5-HT), noradrenaline (NA) and dopamine (DA) levels were quantified on the 1st, 7th and 40th day. 5-HT leve...
journal_title：Toxicology letters
pub_type： 杂志文章
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更新日期：1988-05-01 00:00:00
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journal_title：Toxicology letters
pub_type： 杂志文章
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更新日期：1993-07-01 00:00:00
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journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2017.10.001
更新日期：2017-11-05 00:00:00
abstract：:Mouse neuroblastoma cells exposed to 2.5 and 5.0 microM methylmercury for 24 h appeared rounded with the loss of processes. Immunohistochemical staining directed against beta-tubulin revealed severe alterations in microtubular architecture. Non-membrane-bound condensation product was visualized ultrastructurally in th...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/0378-4274(90)90226-c
更新日期：1990-03-01 00:00:00
abstract：:The target cell(s) of theobromine toxicity on rat testes and reproductive toxicity induced by pure theobromine and cocoa extract are evaluated in the present studies. Theobromine (500 mg/kg x 7 days) inhibited body weight gain in treated rats. Decreased cauda epididymal sperm reserve (38%), seminiferous tubule fluid (...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/0378-4274(94)90159-7
更新日期：1994-02-01 00:00:00
