Abstract:
OBJECTIVE:To investigate the correlation between nasopharyngeal carcinoma cell WNT5A and epithelial-mesenchymal transition (emt)/metastasis, and investigate its possible mechanisms. METHODS:RT-PCR and gene transfection were used to detect the expression of nasopharyngeal carcinoma cell strains WNT5A and EMT related factor 5-8F. Transient transfection of NPC cell line 5-8F was determined by liposome of plasmid with WNT5A gene. The differential expressions of WNT5A and EMT-related factors in cells before and after transfection were detected by RT-PCR. Cell scratch assay and Transwell assay were used to detect the motility abilities of cells before and after 5-8F transfection. RESULTS:The expressions of WNT5A and EMT related factors matrix metalloproteinase-2 of the WNT5A transferred group in the nasopharyngeal carcinoma cell line 5-8F were higher than the blank control group and the empty vector transferred group, and the transfer ability of the WNT5A transferred group was higher than that in the blank control group and the empty vector transferred group, while the expressions of EMT related factors E-cadherin were lower than that in the blank control group and the empty vector transferred group, and the transfer ability of the WNT5A transferred group was higher than that in the blank control group and the empty vector transferred group. CONCLUSIONS:In nasopharyngeal carcinoma cells, WNT5A can regulate the epithelial-mesenchymal transition and affect the ability of tumor invasion and metastasis of nasopharyngeal carcinoma.
journal_name
Asian Pac J Trop Medjournal_title
Asian Pacific journal of tropical medicineauthors
Zhu HH,Zhu XY,Zhou MH,Cheng GY,Lou WHdoi
10.1016/S1995-7645(14)60080-8subject
Has Abstractpub_date
2014-06-01 00:00:00pages
488-91issue
6eissn
1995-7645issn
2352-4146pii
S1995-7645(14)60080-8journal_volume
7pub_type
杂志文章abstract:OBJECTIVE:To evaluate the efficacy and safety of rhPTH (1-34) vs. elcatonin. METHODS:Sixty patients with primary OP were randomly divided into two groups according to the ratio of 3:1. rhPTH (1-34) group (PTH group) was treated with subcutaneous injection of rhPTH (1-34) 20 μ g daily for 18 months, and the elcatonin g...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/S1995-7645(14)60192-9
更新日期:2015-01-01 00:00:00
abstract:OBJECTIVE:To determine Blastocystis frequency and subtypes (ST) in ulcerative colitis (UC) patients and analyse some laboratory findings between Blastocystis positive and negative cases. METHODS:Faecal samples from 150 UC patients in Adnan Menderes University, Training and Research Hospital were examined by direct mic...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/j.apjtm.2016.07.018
更新日期:2016-09-01 00:00:00
abstract:OBJECTIVE:To investigate the protective role of Cardiospermum halicacabum (C. halicacabum) leaf extract on glycoprotein metabolism in streptozotocin (STZ)-induced diabetic rats. METHODS:Diabetes was induced in male albino Wistar rats by intraperitonial administration of STZ. The C. halicacabum leaf extract (CHE) was a...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/S1995-7645(12)60178-3
更新日期:2012-12-01 00:00:00
abstract:OBJECTIVE:To build the rat model of gastric precancerous lesions and discuss the effect of transplantation of mesenchymal stem cells (BMMSCs) on the pathological change. METHODS:The rat model of gastric precancerous lesions was built using N-methyl-N'-nitro-N'-nitrosoguanidine. After the intravenous transplantation of...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章,撤回出版物
doi:10.1016/j.apjtm.2015.11.006
更新日期:2015-12-01 00:00:00
abstract::Toxoplasmosis is a globally distributed parasitic protozoan disease, caused by Toxoplasma gondii. The infection can result in more severe symptoms with potentially life-threatening in case of immunocompromised individuals. Sulfadiazine and pyrimethamine are the two drugs used as a part of standard therapy for toxoplas...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章,评审
doi:10.1016/j.apjtm.2016.06.012
更新日期:2016-08-01 00:00:00
abstract::Salmonella are most commonly associated with gastroenteritis and enteric fever in humans. Occasionally, dissemination of bacilli throughout the body results in establishment of localized foci of persistent infection especially in patients with debilitating diseases and immunosuppressive states. Infection at various ab...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/S1995-7645(11)60117-X
更新日期:2011-05-01 00:00:00
abstract:OBJECTIVE:To evaluate the curative effect of the 132 KD protein isolated from the seeds of Peganum harmala (P. harmala) L. against carbon tetrachloride (CCl(4)) induced oxidative stress in rats. METHODS:Animals were post treated intraperitoneally with 132 KD isolated protein at doses of 4 and 8 mg/kg body weight and b...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/S1995-7645(13)60058-9
更新日期:2013-04-13 00:00:00
abstract:OBJECTIVE:To observe the effect of Rougan Huaqian granules combined with human mesenchymal stem cell (hMSC) transplantation on the liver fibrosis in carbon tetrachloride-induced cirrhosis rats. METHODS:Sixty SD rats were randomly divided into five groups. The rats in control group received intraperitoneal injection of...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/S1995-7645(14)60097-3
更新日期:2014-07-01 00:00:00
abstract:OBJECTIVE:To investigate the mechanism of antibacterial activity of luteolin (LUT) against methicillin-resistant Staphylococcus aureus (MRSA). METHODS:The mechanism of anti-MRSA activity of LUT was analyzed by the viability assay in membrane permeabilizing agent, ATPase inhibitors, and peptidoglycan (PGN) derived from...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/j.apjtm.2015.12.004
更新日期:2016-01-01 00:00:00
abstract:OBJECTIVE:To investigate the efficiency of β-galactosidase gene transfer into rat kidney with ultrasound-mediated microbubble destruction via different injection routes. METHODS:A total of 25 Wistar rats were randomly divided into 5 groups. Four groups received a mixture of optison microbubbles (0.2 mL) and lacz plasm...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/S1995-7645(12)60099-6
更新日期:2012-07-01 00:00:00
abstract:OBJECTIVE:To investigate the pharmacological basis of its uses of Phyla nodiflora (P. nodiflora) for the treatment of anomalies predominantly by smooth muscle containing tissues such as gastrointestinal/vascular/broncho spasm and cardiovascular modulation. METHODS:The crude hydroalcoholic extract of P. nodiflora (Pn.C...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/j.apjtm.2017.10.021
更新日期:2017-12-01 00:00:00
abstract::Tropical pyomyositis is an underdiagnosed condition. We reported a 35 year old male farmer, who presented with septicemia and acute respiratory distress syndrome due to pyomyositis involving the paraspinal muscles. Culture of the pus grew methicillin sensitive Staphylococcus aureus, and the patient recovered after sur...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/S1995-7645(11)60096-5
更新日期:2011-04-01 00:00:00
abstract:OBJECTIVE:To provide further evidence for the ethnomedicinal use of the Eastern Nigeria mistletoe, Loranthus micranthus (L. micranthus), as an immunostimulant. METHODS:Solvent fractions from the crude extract of the mistletoe plant was obtained and screened by the cell mediated delayed type hypersensitivity reaction (...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/S1995-7645(11)60137-5
更新日期:2011-07-01 00:00:00
abstract:OBJECTIVE:To investigate the diuretic, hypotensive and renal effect of Opuntia ficus-indica in two different species in oral and intravenous administration. METHODS:Diuretic activity was evaluated in rats with the plant cladode gel and aqueous extract administrated orally, and was evaluated in rabbits with plant extra...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/j.apjtm.2017.08.016
更新日期:2017-09-01 00:00:00
abstract:OBJECTIVE:To analyze anticancer activity of an ethyl acetate extract of endophytic fungi isolated from soursop leaf (Annona muricata L.). METHODS:Anticancer activity of fungal extracts was determined by observing its toxicity against MCF-7 (Michigan Cancer Foundation-7) cells in vitro by using the 3-(4,5-dimethylthiaz...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/j.apjtm.2017.06.004
更新日期:2017-06-01 00:00:00
abstract:OBJECTIVE:To investigate the antidiabetic effect of aqueous extract of Merremia tridentata (M. tridentata) root (MTRAE) in normal, glucose-loaded hyperglycemic and streptozotocin (STZ)-induced diabetic rats. METHODS:Oral administration of MTRAE at the doses of 50, 100 and 150 mg/kg was studied in normal, glucose-loade...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/S1995-7645(12)60020-0
更新日期:2012-03-01 00:00:00
abstract:OBJECTIVE:To evaluate the antihyperglycemic activity of leaves of Hyptis suaveolens using streptozotocin model. METHODS:Hyptis suaveolens extract (HSE) 250 and 500 mg/kg body weight was administered orally to streptozotocin induced diabetes, once daily for 21 days. RESULTS:A significant reduction in blood glucose was...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/S1995-7645(11)60175-2
更新日期:2011-09-01 00:00:00
abstract:OBJECTIVE:To observe preventive and therapeutic effects and the mechanism of actions on Zishen Yutai pill combined with progesterone on threatened abortion in rats. METHODS:After pregnancy, 50 SPF female SD rats were selected and divided into control group, model group, progesterone group, Zishen Yutai pill group, and...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/j.apjtm.2016.04.002
更新日期:2016-06-01 00:00:00
abstract::Hookworm infection is a relatively common cause of anemia in endemic areas. The most common hookworm species are Ancylostoma duodenale and Necator americanus. In this report we present a case of overt gastrointestinal bleeding because of hookworm infection. Capsule endoscopy revealed many hookworms in the lumen of pro...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/S1995-7645(12)60051-0
更新日期:2012-04-01 00:00:00
abstract:OBJECTIVE:To investigate the effects of exogenous recombinant human brain natriuretic peptide (rhBNP) after primary percutaneous coronary intervention (PCI) on non-invasive hemodynamic in acute myocardial infarction patients with left ventricular failure. METHODS:A number of 96 acute myocardial infarction patients acc...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/j.apjtm.2016.06.006
更新日期:2016-08-01 00:00:00
abstract::A 19-year-old Asian Indian female presented with productive cough since the past one month and low grade fever since the past two weeks. She was diagnosed with pulmonary tuberculosis and treated with antitubercular drugs. Subsequently, delayed cultures of bronchoalveolar lavage fluid grew Burkholderia pseudomallei (B....
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/S1995-7645(14)60031-6
更新日期:2014-03-01 00:00:00
abstract:OBJECTIVE:To confirm dengue infection among Russian tourists returned from Southeast and Mexico in 2010-2013 with clinical signs of infection. METHODS:Blood and serum samples from patients were collected. NS1 antigen and human IgM/IgG antibodies to dengue virus were identified using commercial tests manufactured by "S...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/S1995-7645(14)60194-2
更新日期:2015-02-01 00:00:00
abstract:OBJECTIVE:To study the expression of tumor necrosis factor receptor-associated protein 1 (TRAP1) in gastric cancer tissue and its correlation with malignant biology. METHODS:Gastric cancer tissue and adjacent normal tissue were collected, and mRNA content and protein content of TRAP1 were detected; gastric cancer cell...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/j.apjtm.2015.12.014
更新日期:2016-01-01 00:00:00
abstract:OBJECTIVE:To demonstrate malaria situation analysis, stratification and planning for an endemic area in southern Iran. METHODS:Data on health system, population, meteorological parameters, malaria cases, anopheline vectors, and control activities during 2005-2007 was obtained from Minab Health Center, Minab Meteorolog...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/S1995-7645(11)60036-9
更新日期:2011-01-01 00:00:00
abstract:OBJECTIVE:To investigate the in vitro antioxidant and antiproliferative activity of rhizome extracts of Rhodiola imbricata (R. imbricata) in HT-29 human colon cancer cell line. METHODS:The successively extracted rhizome of R. imbricata using various solvents was analyzed for their total phenolics, tannins and flavonoi...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/S1995-7645(12)60194-1
更新日期:2013-01-01 00:00:00
abstract:OBJECTIVE:To explore the larvicidal and pupicidal activity of Euphorbia hirta (E. hirta) leaf extract and Bacillus sphaericus (B. sphaericus) against the malarial vector, Anopheles stephensi (An. stephensi). METHODS:The larvicidal and pupicidal activity was assayed against An. stephensi at various concentrations rangi...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/S1995-7645(13)60003-6
更新日期:2013-02-01 00:00:00
abstract:OBJECTIVE:To invest the differences among mesenchymal stem cells (MSCs) derived from different tissues and their impacts on clinical applications. METHODS:In this study, MSCs were isolated from adipose tissue (AD), umbilical cord tissue (UC), and menstrual blood (Men) and compared their biological characteristics in t...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/j.apjtm.2015.07.022
更新日期:2015-09-01 00:00:00
abstract:OBJECTIVE:To investigate the feasibility of ultrasonic diagnosis for monitoring fracture healing. METHODS:Thirty rabbit models with fraction of mandible body were established by surgically removing partial lower jawbone. At the 1st, 2nd, 4th, 6th, 8th and 12th week after the operation, they were examined by X-ray and ...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/S1995-7645(12)60068-6
更新日期:2012-05-01 00:00:00
abstract:OBJECTIVE:To investigate the levels of serum cortisol in patients with uncomplicated Plasmodium falciparum (P. falciparum) malaria in an area of unstable malaria transmission in eastern Sudan. METHODS:The concentrations of cortisol were measured in sera of 25 patients with uncomplicated P. falciparum malaria (at prese...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/S1995-7645(11)60056-4
更新日期:2011-02-01 00:00:00
abstract:OBJECTIVE:To explore the role of miR-214 in the progression of hepatocellular carcinoma (HCC) and its inhibitory mechanisms in depressing the signaling pathway of β-catenin, this study was conducted. METHODS:We ectopically expressed miR-214 in HepG2 cells to obtain cell lines Lv-miR-214-HepG2 and their control Lv-cont...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/S1995-7645(14)60350-3
更新日期:2015-05-01 00:00:00