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How many antimicrobial peptide molecules kill a bacterium? The case of PMAP-23.

Abstract：

:Antimicrobial peptides (AMPs) kill bacteria mainly through the perturbation of their membranes and are promising compounds to fight drug resistance. Models of the mechanism of AMPs-induced membrane perturbation were developed based on experiments in liposomes, but their relevance for bacterial killing is debated. We determined the association of an analogue of the AMP PMAP-23 to Escherichia coli cells, under the same experimental conditions used to measure bactericidal activity. Killing took place only when bound peptides completely saturated bacterial membranes (10(6)-10(7) bound peptides per cell), indicating that the "carpet" model for the perturbation of artificial bilayers is representative of what happens in real bacteria. This finding supports the view that, at least for this peptide, a microbicidal mechanism is possible in vivo only at micromolar total peptide concentrations. We also showed that, notwithstanding their simplicity, liposomes represent a reliable model to characterize AMPs partition in bacterial membranes.

journal_name

ACS Chem Biol

journal_title

ACS chemical biology

authors

Roversi D,Luca V,Aureli S,Park Y,Mangoni ML,Stella L

doi

10.1021/cb500426r

subject

Has Abstract

pub_date

2014-09-19 00:00:00

pages

2003-7

issue

9

eissn

1554-8929

issn

1554-8937

journal_volume

9

pub_type

信件

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