Enhanced receptor-clathrin interactions induced by N-glycan-mediated membrane micropatterning.

Abstract:

:Glycan-protein interactions are emerging as important modulators of membrane protein organization and dynamics, regulating multiple cellular functions. In particular, it has been postulated that glycan-mediated interactions regulate surface residence time of glycoproteins and endocytosis. How this precisely occurs is poorly understood. Here we applied single-molecule-based approaches to directly visualize the impact of glycan-based interactions on the spatiotemporal organization and interaction with clathrin of the glycosylated pathogen recognition receptor dendritic cell-specific intercellular adhesion molecule-3-grabbing nonintegrin (DC-SIGN). We find that cell surface glycan-mediated interactions do not influence the nanoscale lateral organization of DC-SIGN but restrict the mobility of the receptor to distinct micrometer-size membrane regions. Remarkably, these regions are enriched in clathrin, thereby increasing the probability of DC-SIGN-clathrin interactions beyond random encountering. N-glycan removal or neutralization leads to larger membrane exploration and reduced interaction with clathrin, compromising clathrin-dependent internalization of virus-like particles by DC-SIGN. Therefore, our data reveal that cell surface glycan-mediated interactions add another organization layer to the cell membrane at the microscale and establish a novel mechanism of extracellular membrane organization based on the compartments of the membrane that a receptor is able to explore. Our work underscores the important and complex role of surface glycans regulating cell membrane organization and interaction with downstream partners.

authors

Torreno-Pina JA,Castro BM,Manzo C,Buschow SI,Cambi A,Garcia-Parajo MF

doi

10.1073/pnas.1402041111

subject

Has Abstract

pub_date

2014-07-29 00:00:00

pages

11037-42

issue

30

eissn

0027-8424

issn

1091-6490

pii

1402041111

journal_volume

111

pub_type

杂志文章
  • Determination of water mass properties of water pumped down from the Ekman layer to the geostrophic flow below.

    abstract::In a subtropical gyre, the convergent Ekman layer forces water downward into the geostrophic flow below. The properties and depth of the mixed layer vary considerably during the course of the year, but this variability does not penetrate into the geostrophic region. Evidently there is some process at work that selects...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.76.7.3051

    authors: Stommel H

    更新日期:1979-07-01 00:00:00

  • Disruption of an intersubunit electrostatic bond is a critical step in glycine receptor activation.

    abstract::Proper regulation of neurotransmission requires that ligand-activated ion channels remain closed until agonist binds. How channels then open remains poorly understood. Glycine receptor (GlyR) gating is initiated by agonist binding at interfaces between adjacent subunits in the extracellular domain. Aspartate-97, locat...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1001845107

    authors: Todorovic J,Welsh BT,Bertaccini EJ,Trudell JR,Mihic SJ

    更新日期:2010-04-27 00:00:00

  • Mapping methyl jasmonate-mediated transcriptional reprogramming of metabolism and cell cycle progression in cultured Arabidopsis cells.

    abstract::Jasmonates (JAs) are plant-specific signaling molecules that steer a diverse set of physiological and developmental processes. Pathogen attack and wounding inflicted by herbivores induce the biosynthesis of these hormones, triggering defense responses both locally and systemically. We report on alterations in the tran...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0711203105

    authors: Pauwels L,Morreel K,De Witte E,Lammertyn F,Van Montagu M,Boerjan W,Inzé D,Goossens A

    更新日期:2008-01-29 00:00:00

  • Genomic linkage of male song and female acoustic preference QTL underlying a rapid species radiation.

    abstract::The genetic coupling hypothesis of signal-preference evolution, whereby the same genes control male signal and female preference for that signal, was first inspired by the evolution of cricket acoustic communication nearly 50 years ago. To examine this hypothesis, we compared the genomic location of quantitative trait...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0900229106

    authors: Shaw KL,Lesnick SC

    更新日期:2009-06-16 00:00:00

  • Linking plasma formation in grapes to microwave resonances of aqueous dimers.

    abstract::The sparking of cut grape hemispheres in a household microwave oven has been a poorly explained Internet parlor trick for over two decades. By expanding this phenomenon to whole spherical dimers of various grape-sized fruit and hydrogel water beads, we demonstrate that the formation of plasma is due to electromagnetic...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1818350116

    authors: Khattak HK,Bianucci P,Slepkov AD

    更新日期:2019-03-05 00:00:00

  • Extreme rainfall activity in the Australian tropics reflects changes in the El Niño/Southern Oscillation over the last two millennia.

    abstract::Assessing temporal variability in extreme rainfall events before the historical era is complicated by the sparsity of long-term "direct" storm proxies. Here we present a 2,200-y-long, accurate, and precisely dated record of cave flooding events from the northwest Australian tropics that we interpret, based on an integ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1422270112

    authors: Denniston RF,Villarini G,Gonzales AN,Wyrwoll KH,Polyak VJ,Ummenhofer CC,Lachniet MS,Wanamaker AD Jr,Humphreys WF,Woods D,Cugley J

    更新日期:2015-04-14 00:00:00

  • Regulation of hematopoiesis by the K63-specific ubiquitin-conjugating enzyme Ubc13.

    abstract::The ubiquitin-conjugating enzyme Ubc13 mediates lysine-63-specific protein ubiquitination involved in signal transduction by immune receptors; however, the in vivo physiological functions of Ubc13 remain incompletely understood. Using Ubc13 conditional knockout mice, we show that somatic deletion of the Ubc13 gene cau...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0906547106

    authors: Wu X,Yamamoto M,Akira S,Sun SC

    更新日期:2009-12-08 00:00:00

  • Creating ribosomes with an all-RNA 30S subunit P site.

    abstract::Ribosome crystal structures have revealed that two small subunit proteins, S9 and S13, have C-terminal tails, which, together with several features of 16S rRNA, contact the anticodon stem-loop of P-site tRNA. To test the functional importance of these protein tails, we created genomic deletions of the C-terminal regio...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0405227101

    authors: Hoang L,Fredrick K,Noller HF

    更新日期:2004-08-24 00:00:00

  • Dynamics of living cells in a cytomorphological state space.

    abstract::Cells are nonequilibrium systems that exchange matter and energy with the environment to sustain their metabolic needs. The nonequilibrium nature of this system presents considerable challenges to developing a general theory describing its behavior; however, when studied at appropriate spatiotemporal scales, the behav...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1902849116

    authors: Chang AY,Marshall WF

    更新日期:2019-10-22 00:00:00

  • Epidermal growth factor regulates the expression of its own receptor.

    abstract::The epidermal growth factor (EGF) receptor gene is the cellular homolog of the avian erythroblastosis virus erbB oncogene. Control of EGF receptor expression determines cellular responsiveness to EGF and might play an important role in neoplastic development. Using RNA blot hybridization, we have found that exposure o...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.82.24.8374

    authors: Clark AJ,Ishii S,Richert N,Merlino GT,Pastan I

    更新日期:1985-12-01 00:00:00

  • Elimination of zinc from synaptic vesicles in the intact mouse brain by disruption of the ZnT3 gene.

    abstract::The mammalian protein ZnT3 resides on synaptic vesicle membranes of zinc-containing neurons, suggesting its possible role in vesicular zinc transport. We show here that histochemically reactive zinc, corresponding to the zinc found within synaptic vesicles, was undetectable in the brains of mice with targeted disrupti...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.96.4.1716

    authors: Cole TB,Wenzel HJ,Kafer KE,Schwartzkroin PA,Palmiter RD

    更新日期:1999-02-16 00:00:00

  • Structure and mechanism of mouse cysteine dioxygenase.

    abstract::Cysteine dioxygenase (CDO) catalyzes the oxidation of l-cysteine to cysteine sulfinic acid. Deficiencies in this enzyme have been linked to autoimmune diseases and neurological disorders. The x-ray crystal structure of CDO from Mus musculus was solved to a nominal resolution of 1.75 Angstroms. The sequence is 91% iden...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0509262103

    authors: McCoy JG,Bailey LJ,Bitto E,Bingman CA,Aceti DJ,Fox BG,Phillips GN Jr

    更新日期:2006-02-28 00:00:00

  • Is there a single pathway for the folding of a polypeptide chain?

    abstract::We argue that folding of the compact domains of proteins can occur with adequate rapidity in the absence of a unique directed mechanism, provided that native-like local structure dominates the folding process. We further suggest that the evolution of amino acid sequences should favor multiple paths to the folded state...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.82.12.4028

    authors: Harrison SC,Durbin R

    更新日期:1985-06-01 00:00:00

  • The Xist RNA-PRC2 complex at 20-nm resolution reveals a low Xist stoichiometry and suggests a hit-and-run mechanism in mouse cells.

    abstract::X-chromosome inactivation (XCI) is initiated by the long noncoding RNA Xist, which coats the inactive X (Xi) and targets Polycomb repressive complex 2 (PRC2) in cis. Epigenomic analyses have provided significant insight into Xist binding patterns and chromatin organization of the Xi. However, such epigenomic analyses ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1503690112

    authors: Sunwoo H,Wu JY,Lee JT

    更新日期:2015-08-04 00:00:00

  • The anaphase promoting complex/cyclosome is required during development for modified cell cycles.

    abstract::Animals and plants use modified cell cycles to achieve particular developmental strategies. In one common example, most animals and plants have tissues in which the cells become polyploid or polytene by means of an S-G cycle, but the mechanism by which mitosis is inhibited in the endo cycle is not understood. The Dros...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.172391099

    authors: Kashevsky H,Wallace JA,Reed BH,Lai C,Hayashi-Hagihara A,Orr-Weaver TL

    更新日期:2002-08-20 00:00:00

  • Improved predictions of secondary structures for RNA.

    abstract::The accuracy of computer predictions of RNA secondary structure from sequence data and free energy parameters has been increased to roughly 70%. Performance is judged by comparison with structures known from phylogenetic analysis. The algorithm also generates suboptimal structures. On average, the best structure withi...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.86.20.7706

    authors: Jaeger JA,Turner DH,Zuker M

    更新日期:1989-10-01 00:00:00

  • Regulation of hemoglobin synthesis in a murine erythroblastic leukemic cell: the requirement for replication to induce hemoglobin synthesis.

    abstract::The induction of hemoglobin synthesis by dimethylsulfoxide in cells infected with Friend leukemia virus has been shown to depend on the number of cell divisions after the addition of the inducing agent. In asynchronous cultures the cells have a generation time of 24 hr, and hemoglobin synthesis occurs after 48 hr in t...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.71.11.4551

    authors: McClintock PR,Papaconstantinou J

    更新日期:1974-11-01 00:00:00

  • Systematic, genome-wide identification of host genes affecting replication of a positive-strand RNA virus.

    abstract::Positive-strand RNA viruses are the largest virus class and include many pathogens such as hepatitis C virus and the severe acute respiratory syndrome coronavirus (SARS). Brome mosaic virus (BMV) is a representative positive-strand RNA virus whose RNA replication, gene expression, and encapsidation have been reproduce...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.2536857100

    authors: Kushner DB,Lindenbach BD,Grdzelishvili VZ,Noueiry AO,Paul SM,Ahlquist P

    更新日期:2003-12-23 00:00:00

  • DNA-membrane association is necessary for initiation of chromosomal and plasmid replication in Bacillus subtilis.

    abstract::We examined the effect of the inhibition of initiation of DNA replication on the membrane association of the chromosomal origin of replication of Bacillus subtilis and the Staphylococcus aureus-Bacillus pumilus chimeric plasmid pSL103, using temperature-sensitive mutants of B. subtilis that have specifically affected ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.77.5.2834

    authors: Winston S,Sueoka N

    更新日期:1980-05-01 00:00:00

  • Material-based deployment enhances efficacy of endothelial progenitor cells.

    abstract::Cell-based therapies are attractive for revascularizing and regenerating tissues and organs, but clinical trials of endothelial progenitor cell transplantation have not resulted in consistent benefit. We propose a different approach in which a material delivery system is used to create a depot of vascular progenitor c...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0803873105

    authors: Silva EA,Kim ES,Kong HJ,Mooney DJ

    更新日期:2008-09-23 00:00:00

  • Human ribosomal RNA gene: nucleotide sequence of the transcription initiation region and comparison of three mammalian genes.

    abstract::The transcription initiation site of the human ribosomal RNA gene (rDNA) was located by using the single-strand specific nuclease protection method and by determining the first nucleotide of the in vitro capped 45S preribosomal RNA. The sequence of 1,211 nucleotides surrounding the initiation site was determined. The ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.79.10.3092

    authors: Financsek I,Mizumoto K,Mishima Y,Muramatsu M

    更新日期:1982-05-01 00:00:00

  • Site-specific gene delivery in vivo through engineered Sendai viral envelopes.

    abstract::Inspite of several stimulating developments in gene therapy, the formulation of a targeted gene delivery "vector" is still far from ideal. We have demonstrated the potential of reconstituted Sendai viral envelopes containing only the fusion glycoprotein (F-virosomes) in targeted delivery of reporter genes to liver cel...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.95.20.11886

    authors: Ramani K,Hassan Q,Venkaiah B,Hasnain SE,Sarkar DP

    更新日期:1998-09-29 00:00:00

  • Suppression of EGFR endocytosis by dynamin depletion reveals that EGFR signaling occurs primarily at the plasma membrane.

    abstract::The role of endocytosis in the control of EGF receptor (EGFR) activation and cell signaling was explored by using mouse fibroblasts in which dynamin was conditionally depleted. Dynamin is a GTPase shown to play an important role in the control clathrin mediated endocytosis of EGFR and other cell surface receptors. In ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1200164109

    authors: Sousa LP,Lax I,Shen H,Ferguson SM,De Camilli P,Schlessinger J

    更新日期:2012-03-20 00:00:00

  • Anaerobic transport in Escherichia coli membrane vesicles.

    abstract::Anaerobic beta-galactoside transport in whole cells and membrane vesicles from E. coli ML 308-225 is coupled to the oxidation of alpha-glycerol-P or D-lactate with fumarate as an electron acceptor. Alternatively, anaerobic beta-galactoside transport may be coupled to the oxidation of formate utilizing nitrate as elect...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.70.12.3376

    authors: Konings WN,Kaback HR

    更新日期:1973-12-01 00:00:00

  • Action control is mediated by prefrontal BDNF and glucocorticoid receptor binding.

    abstract::Stressor exposure biases decision-making strategies from those based on the relationship between actions and their consequences to others restricted by stimulus-response associations. Chronic stressor exposure also desensitizes glucocorticoid receptors (GR) and diminishes motivation to acquire food reinforcement, alth...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1208342109

    authors: Gourley SL,Swanson AM,Jacobs AM,Howell JL,Mo M,Dileone RJ,Koleske AJ,Taylor JR

    更新日期:2012-12-11 00:00:00

  • Biosynthesis of mouse interferon by translation of its messenger RNA in a cell-free system.

    abstract::A fraction of mouse RNA containing messenger RNA coding for mouse interferon was translated with high efficiency in a wheat germ system into a fully active product. This product fulfills the criteria for mouse interferon, namely: (1) it was active against vesicular stomatitis virus and herpes simplex virus in mouse ce...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.72.10.3975

    authors: Thang MN,Thang DC,De Maeyer E,Montagnier L

    更新日期:1975-10-01 00:00:00

  • Dimer formation drives the activation of the cell death protease caspase 9.

    abstract::A critical step in the induction of apoptosis is the activation of the apoptotic initiator caspase 9. We show that at its normal physiological concentration, caspase 9 is primarily an inactive monomer (zymogen), and that activity is associated with a dimeric species. At the high concentrations used for crystal formati...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.231465798

    authors: Renatus M,Stennicke HR,Scott FL,Liddington RC,Salvesen GS

    更新日期:2001-12-04 00:00:00

  • In vitro assembly of a functional nucleocapsid from the negative-stranded genome RNA of a defective interfering particle of vesicular stomatitis virus.

    abstract::The template for transcription and replication of negative-stranded RNA viruses is a ribonucleoprotein structure, the nucleocapsid. We have developed a system that supports assembly of the negative-stranded RNA genome of a defective interfering (DI) particle of vesicular stomatitis virus (VSV) into a nucleocapsid in v...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.85.20.7511

    authors: Mirakhur B,Peluso RW

    更新日期:1988-10-01 00:00:00

  • Classical conditioning induces long-term translocation of protein kinase C in rabbit hippocampal CA1 cells.

    abstract::The role of the Ca2+/phospholipid-dependent, diacylglycerol-activated enzyme protein kinase C (PKC) in rabbit eyelid conditioning was examined. PKC was partially purified from the CA1 region of hippocampal slices from naive, pseudoconditioned, and conditioned rabbits 24 hr after the rabbits were well conditioned. Crud...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.85.6.1988

    authors: Bank B,DeWeer A,Kuzirian AM,Rasmussen H,Alkon DL

    更新日期:1988-03-01 00:00:00

  • Serotonin receptor-mediated stimulation of bovine smooth muscle cell prostacyclin synthesis and its modulation by platelet-derived growth factor.

    abstract::Serotonin (5-hydroxytryptamine; 0.5 microM and above) stimulated the synthesis of prostacyclin (as measured by radioimmunoassay of 6-ketoprostaglandin F1 alpha) by bovine aortic smooth muscle cells in culture. This effect was structurally specific; a similar response was not elicited by the other indoles (tryptophan, ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.78.11.7134

    authors: Coughlin SR,Moskowitz MA,Antoniades HN,Levine L

    更新日期:1981-11-01 00:00:00