Abstract:
:Whole exome sequencing is a technique that aims to selectively sequence all exons of protein-coding genes. A canine whole exome sequencing enrichment kit was designed based on the latest canine reference genome (build 3.1.72). Its performance was tested by sequencing 2 exome captures, each consisting of 4 pre-capture pooled, barcoded Illumina libraries on an Illumina HiSeq 2500. At an average sequencing depth of 102x, 83 to 86% of the target regions were completely sequenced with a minimum coverage of five and 90% of the reads mapped on the target regions. Additionally, it is shown that the reproducibility within and between captures is high and that pooling four samples per capture is a valid option. Overall, we have demonstrated the strong performance of this WES enrichment kit and are confident it will be a valuable tool in future disease association studies.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Broeckx BJ,Coopman F,Verhoeven GE,Bavegems V,De Keulenaer S,De Meester E,Van Niewerburgh F,Deforce Ddoi
10.1038/srep05597subject
Has Abstractpub_date
2014-07-07 00:00:00pages
5597issn
2045-2322pii
srep05597journal_volume
4pub_type
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