Abstract:
:Chromatin regulation is driven by multicomponent protein complexes, which form functional modules. Deciphering the components of these modules and their interactions is central to understanding the molecular pathways these proteins are regulating, their functions, and their relation to both normal development and disease. We describe the use of affinity purifications of tagged human proteins coupled with mass spectrometry to generate a protein-protein interaction map encompassing known and predicted chromatin-related proteins. On the basis of 1,394 successful purifications of 293 proteins, we report a high-confidence (85% precision) network involving 11,464 protein-protein interactions among 1,738 different human proteins, grouped into 164 often overlapping protein complexes with a particular focus on the family of JmjC-containing lysine demethylases, their partners, and their roles in chromatin remodeling. We show that RCCD1 is a partner of histone H3K36 demethylase KDM8 and demonstrate that both are important for cell-cycle-regulated transcriptional repression in centromeric regions and accurate mitotic division.
journal_name
Cell Repjournal_title
Cell reportsauthors
Marcon E,Ni Z,Pu S,Turinsky AL,Trimble SS,Olsen JB,Silverman-Gavrila R,Silverman-Gavrila L,Phanse S,Guo H,Zhong G,Guo X,Young P,Bailey S,Roudeva D,Zhao D,Hewel J,Li J,Gräslund S,Paduch M,Kossiakoff AA,Lupien M,doi
10.1016/j.celrep.2014.05.050subject
Has Abstractpub_date
2014-07-10 00:00:00pages
297-310issue
1issn
2211-1247pii
S2211-1247(14)00444-6journal_volume
8pub_type
杂志文章相关文献
Cell Reports文献大全abstract::One of the most notable characteristics of synaptic transmission is the wide variation in synaptic strength in response to identical stimulation. In hippocampal neurons, approximately one-third of axonal mitochondria are highly motile, and some dynamically pass through presynaptic boutons. This raises a fundamental qu...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2013.06.040
更新日期:2013-08-15 00:00:00
abstract::Pre-implantation embryo development is an intricate and precisely regulated process orchestrated by maternally inherited proteins and newly synthesized proteins following zygotic genome activation. Although genomic and transcriptomic studies have enriched our understanding of the genetic programs underlying this proce...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2017.11.111
更新日期:2017-12-26 00:00:00
abstract::Memory B cells (MBCs) epitomize the adaptation of the immune system to the environment. We identify two MBC subsets in peripheral blood, CD27dull and CD27bright MBCs, whose frequency changes with age. Heavy chain variable region (VH) usage, somatic mutation frequency replacement-to-silent ratio, and CDR3 property chan...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2020.02.022
更新日期:2020-03-03 00:00:00
abstract::Senescence is a cellular response preventing tumorigenesis. The Ras oncogene is frequently activated or mutated in human cancers, but Ras activation is insufficient to transform primary cells. In a search for cooperating oncogenes, we identify the lysine demethylase JMJD2A/KDM4A. We show that JMJD2A functions as a neg...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2012.09.033
更新日期:2012-11-29 00:00:00
abstract::T and innate lymphoid cells (ILCs) share some aspects of their developmental programs. However, although Notch signaling is strictly required for T cell development, it is dispensable for fetal ILC development. Constitutive activation of Notch signaling, at the common lymphoid progenitor stage, drives T cell developme...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2016.01.015
更新日期:2016-02-16 00:00:00
abstract::Interleukin (IL)-17 signaling to the intestinal epithelium regulates the intestinal microbiome. Given the reported links between intestinal dysbiosis, bacterial translocation, and liver disease, we hypothesize that intestinal IL-17R signaling plays a critical role in mitigating hepatic inflammation. To test this, we s...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2019.10.042
更新日期:2019-11-19 00:00:00
abstract::The serine/threonine protein phosphatase 5 (PP5) regulates multiple cellular signaling networks. A number of cellular factors, including heat shock protein 90 (Hsp90), promote the activation of PP5. However, it is unclear whether post-translational modifications also influence PP5 phosphatase activity. Here, we show a...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2017.10.074
更新日期:2017-11-14 00:00:00
abstract::There is an urgent need to identify new treatments for fungal infections. By combining sub-lethal concentrations of the known antifungals fluconazole, caspofungin, amphotericin B, terbinafine, benomyl, and cyprodinil with ∼3,600 compounds in diverse fungal species, we generated a deep reservoir of chemical-chemical in...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2015.10.018
更新日期:2015-11-17 00:00:00
abstract::Glycophosphatidylinositol-anchored proteins (GPI-APs) play essential roles in physiology, but their biogenesis and trafficking have not been systematically characterized. Here, we took advantage of the recently available haploid genetics approach to dissect GPI-AP pathways in human cells using prion protein (PrP) and ...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2015.05.026
更新日期:2015-06-23 00:00:00
abstract::In neurons, transmembrane proteins are targeted to dendrites in vesicles that traffic solely within the somatodendritic compartment. How these vesicles are retained within the somatodendritic domain is unknown. Here, we use a novel pulse-chase system, which allows synchronous release of exogenous transmembrane protein...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2012.05.018
更新日期:2012-07-26 00:00:00
abstract::Manipulation of the master regulator of energy homeostasis AMP-activated protein kinase (AMPK) activity is a strategy used by many intracellular pathogens for successful replication. Infection by most pathogens leads to an activation of host AMPK activity due to the energetic demands placed on the infected cell. Here,...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2016.08.001
更新日期:2016-09-06 00:00:00
abstract::Transcription factors often activate and repress different target genes in the same cell. How activation and repression are encoded by different arrangements of transcription factor binding sites in cis-regulatory elements is poorly understood. We investigated how sites for the transcription factor CRX encode both act...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2016.09.066
更新日期:2016-10-25 00:00:00
abstract::The architecture of renal glomeruli is acquired through intricate and still poorly understood developmental steps. In our study we identify a crucial glomerular morphogenetic event in nephrogenesis that drives the remodeling/separation of the prospective vascular pole (the future entrance of the glomerular arterioles)...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2020.108304
更新日期:2020-10-27 00:00:00
abstract::Lymphatic vascular development establishes embryonic and adult tissue fluid balance and is integral in disease. In diverse vertebrate organs, lymphatic vessels display organotypic function and develop in an organ-specific manner. In all settings, developmental lymphangiogenesis is considered driven by vascular endothe...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2019.07.055
更新日期:2019-08-20 00:00:00
abstract::Human brain imaging studies have revealed the anterior cingulate cortex (ACC) as a key brain region for mediating visceral-pain-cognitive interactions. Recently, we characterized impairments of long-term potentiation and spike-field coherence in the basolateral amygdala (BLA)-ACC network in association with a decision...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2017.11.012
更新日期:2017-11-28 00:00:00
abstract::Pancreatic α cells may process proglucagon not only to glucagon but also to glucagon-like peptide-1 (GLP-1). However, the biological relevance of paracrine GLP-1 for β cell function remains unclear. We studied effects of locally derived insulin secretagogues on β cell function and glucose homeostasis using mice with α...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2017.03.005
更新日期:2017-03-28 00:00:00
abstract::Characterization of the cardiac cellulome, the network of cells that form the heart, is essential for understanding cardiac development and normal organ function and for formulating precise therapeutic strategies to combat heart disease. Recent studies have reshaped our understanding of cardiac cellular composition an...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2017.12.072
更新日期:2018-01-16 00:00:00
abstract::Granule cells (GCs) of the cerebellar input layer express high-affinity δ GABAA subunit-containing GABAA receptors (δGABAARs) that respond to ambient GABA levels and context-dependent neuromodulators like steroids. We find that GC-specific deletion of δGABAA (cerebellar [cb] δ knockout [KO]) decreases tonic inhibition...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2020.108338
更新日期:2020-11-03 00:00:00
abstract::Mutations in BLM helicase predispose Bloom syndrome (BS) patients to a wide spectrum of cancers. We demonstrate that MIB1-ubiquitylated BLM in G1 phase functions as an adaptor protein by enhancing the binding of transcription factor c-Jun and its E3 ligase, Fbw7α. BLM enhances the K48/K63-linked ubiquitylation on c-Ju...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2018.06.101
更新日期:2018-07-24 00:00:00
abstract::Although it is well established that tumor-associated macrophages take part in each step of cancer progression, less is known about the distinct role of the so-called metastasis-associated macrophages (MAMs) at the metastatic site. Previous studies reported that Caveolin-1 (Cav1) has both tumor-promoting and tumor-sup...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2017.11.034
更新日期:2017-12-05 00:00:00
abstract::Cyclin D1 encodes the regulatory subunit of a holoenzyme that phosphorylates RB and functions as a collaborative nuclear oncogene. The serine threonine kinase Akt plays a pivotal role in the control of cellular metabolism, survival, and mitogenic signaling. Herein, Akt1-mediated phosphorylation of downstream substrate...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2020.108151
更新日期:2020-09-15 00:00:00
abstract::Recent rapid progress in the field of mechanobiology has been driven by novel emerging tools and methodologies and growing interest from different scientific disciplines. Specific progress has been made toward understanding how cell mechanics is linked to intracellular signaling and the regulation of gene expression i...
journal_title:Cell reports
pub_type: 杂志文章,评审
doi:10.1016/j.celrep.2019.08.075
更新日期:2019-10-01 00:00:00
abstract::Inflammatory bowel disease (IBD) is prevalent, but the mechanisms underlying disease development remain elusive. We identify a role for the E3 ubiquitin ligase RNF5 in IBD. Intestinal epithelial cells (IECs) express a high level of RNF5, while the colon of Rnf5-/- mice exhibits activated dendritic cells and intrinsic ...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2018.08.057
更新日期:2018-09-18 00:00:00
abstract::Many infections are caused by pathogens that are similar, but not identical, to previously encountered viruses, bacteria, or vaccines. In such re-infections, pathogens introduce known antigens, which are recognized by memory T cells and new antigens that activate naive T cells. How preexisting memory T cells impact th...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2016.09.072
更新日期:2016-10-11 00:00:00
abstract::Zinc-finger E-box binding homeobox 1 (Zeb1) is a key regulator of epithelial-mesenchymal transition and cancer metastasis. Mutation of ZEB1 is associated with human diseases and defective brain development. Here we show that downregulation of Zeb1 expression in embryonic cortical neural progenitor cells (NPCs) is nece...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2019.04.081
更新日期:2019-05-21 00:00:00
abstract::Spondweni virus (SPOV) is the flavivirus that is most closely related to Zika virus (ZIKV). Although SPOV causes sporadic human infections in Africa, recently it was found in Culex mosquitoes in Haiti. To investigate the pathogenic spectrum of SPOV, we developed infection models in mice. Although two SPOV strains fail...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2019.01.052
更新日期:2019-02-05 00:00:00
abstract::Mutations in WNK kinases cause the human hypertensive disease pseudohypoaldosteronism type II (PHAII), but the regulatory mechanisms of the WNK kinases are not well understood. Mutations in kelch-like 3 (KLHL3) and Cullin3 were also recently identified as causing PHAII. Therefore, new insights into the mechanisms of h...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2013.02.024
更新日期:2013-03-28 00:00:00
abstract::Insulin-stimulated hepatic glycogen synthesis is central to glucose homeostasis. Here, we show that PPP1R3G, a regulatory subunit of protein phosphatase 1 (PP1), is directly phosphorylated by AKT. PPP1R3G phosphorylation fluctuates with fasting-refeeding cycle and is required for insulin-stimulated dephosphorylation, ...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2019.08.066
更新日期:2019-09-24 00:00:00
abstract::We report the emergence of an endogenous circadian clock that regulates organogenesis in mouse fetal kidney. We detect circadian rhythms both in vivo with transcriptional profiling and ex vivo by bioluminescence. High-resolution structural analysis of embryonic explants reveals that global or local clock disruption re...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2020.107661
更新日期:2020-05-19 00:00:00
abstract::Vesicular glutamate transporters (VGLUTs) fill synaptic vesicles with glutamate and are thus essential for glutamatergic neurotransmission. However, VGLUTs were originally discovered as members of a transporter subfamily specific for inorganic phosphate (Pi). It is still unclear how VGLUTs accommodate glutamate transp...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2018.03.055
更新日期:2018-04-10 00:00:00