Abstract:
OBJECTIVE:To evaluate the degree of enrichment of spermatogonial stem cells (SSCs) from human testicular cell cultures by ITGA6+, HLA-/ITGA6+, GPR125+, and HLA-/GPR125+ magnetic-assisted cell sorting (MACS). DESIGN:Experimental basic science study. SETTING:Reproductive biology laboratory. PATIENT(S):Multiple samples of cryopreserved human testicular cells from two prostate cancer patients with normal spermatogenesis. INTERVENTION(S):Cultured human testicular cells subjected to four sorting strategies based on MACS and xenotransplanted to the testes of mice to determine the enrichment for SSCs. MAIN OUTCOME MEASURE(S):Enrichment for human spermatogonia and SSCs tested by expression analysis of spermatogonial markers ITGA6, GPR125, ZBTB16, UCHL1, and ID4 using quantitative real-time polymerase chain reaction (qPCR) and by xenotransplantation into the testes of mice, respectively. RESULT(S):Compared with the nonsorted cultured testicular cells, only the ITGA6+ and HLA-/GPR125+ sorted cells showed enrichment for ID4. No difference in expression of ZBTB16 and UCHL1 was observed. Xenotransplantation of the sorted cell fractions showed a 7.1-fold enrichment of SSCs with ITGA6+. CONCLUSION(S):Magnetic-assisted cell sorting of cultured human testicular cells using ITGA6 allows for enrichment of SSCs, which aids in further molecular characterization of cultured human SSCs and enhances testicular colonization upon transplantation in future clinical settings.
journal_name
Fertil Steriljournal_title
Fertility and sterilityauthors
Nickkholgh B,Mizrak SC,Korver CM,van Daalen SK,Meissner A,Repping S,van Pelt AMdoi
10.1016/j.fertnstert.2014.04.022subject
Has Abstractpub_date
2014-08-01 00:00:00pages
558-565.e5issue
2eissn
0015-0282issn
1556-5653pii
S0015-0282(14)00382-3journal_volume
102pub_type
杂志文章abstract::In this prospective, randomized study, waiting for the lead follicle to reach 14 mm before initiating GnRH antagonist administration effectively prevents an LH surge and ovulation during an IVF cycle. Furthermore, delaying GnRH start until the dominant follicle reaches 14 mm neither impacts the clinical pregnancy, imp...
journal_title:Fertility and sterility
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.fertnstert.2009.05.083
更新日期:2009-12-01 00:00:00
abstract:OBJECTIVE:To study the correlation between the incidence of sex chromosome aneuploidies in the somatic cells and spermatozoa in karyotypically normal infertile men and fertile donors. DESIGN:A prospective, phase two, controlled study. SETTING:A teaching Hospital Reproductive Medicine and Medical Genetics Units. PATI...
journal_title:Fertility and sterility
pub_type: 临床试验,杂志文章
doi:10.1016/s0015-0282(00)00603-8
更新日期:2000-08-01 00:00:00
abstract:OBJECTIVE:To evaluate the effect of metformin in polycystic ovary syndrome (PCOS). As follow-up on a previous paper describing hormonal and metabolic factors, this paper focuses on correlations between adiponectin and anthropometric, hormonal, and metabolic factors in PCOS and the effect of metformin. DESIGN:Randomize...
journal_title:Fertility and sterility
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.fertnstert.2010.01.057
更新日期:2010-11-01 00:00:00
abstract:OBJECTIVE:To assess the safety and effectiveness of a sprayable, site-specific adhesion barrier system (SprayGel; Confluent Surgical, Waltham, MA) for reduction of adhesion formation. DESIGN:Prospective, randomized, controlled phase III trial. SETTING:University clinic (Kiel, Germany) and private clinic (Bordeaux, Fr...
journal_title:Fertility and sterility
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:10.1016/j.fertnstert.2003.12.046
更新日期:2004-08-01 00:00:00
abstract:OBJECTIVE:To determine the clinical significance of albumin determination in ejaculates by means of an easy office test to screen semen samples for subclinical infection-inflammation. PATIENTS:One hundred fifty-nine randomly chosen males of couples with longstanding infertility (median duration of infertility 4 years ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:
更新日期:1995-11-01 00:00:00
abstract::Neutrophil migration did not change during normal menstrual cycles but was significantly reduced during controlled ovarian hyperstimulation (COH). Correlations between neutrophil migration and serum hormone concentrations could not be established. ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2004.05.091
更新日期:2004-12-01 00:00:00
abstract:OBJECTIVE:This study was designed to assess the ovarian response in the same patient in consecutive IVF cycles. DESIGN:Retrospective study. SETTING:Assisted reproductive unit at a university hospital. PATIENT(S):One hundred ninety women who underwent three consecutive cycles of IVF. INTERVENTION(S):All women used a...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(01)03237-x
更新日期:2002-04-01 00:00:00
abstract:OBJECTIVE:To evaluate the association between variants in the FSHR receptor (FSHR) gene and current markers of ovarian reserve (antimüllerian hormone, antral follicle count, FSH). DESIGN:Prospective observational study. SETTING:Tertiary referral center for reproductive medicine. PATIENT(S):Women (n = 421) undergoing...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2011.12.040
更新日期:2012-03-01 00:00:00
abstract:OBJECTIVE:To investigate the usefulness of dual-parameter flow cytometry of testicular fine-needle aspirates for the quantification of spermatogenesis. Immunofluorescence staining for vimentin was introduced to discriminate vimentin-negative germ cells from vimentin-positive stromal cells. The results of flow cytometry...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)56656-4
更新日期:1994-04-01 00:00:00
abstract:OBJECTIVE:To evaluate whether overweight and obesity are related to changes in serum sex hormone concentrations and semen quality. DESIGN:Cross-sectional study. SETTING:Data from five previous population-based environmental studies of semen quality were combined into one database at a university hospital in Denmark. ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2007.07.1292
更新日期:2008-09-01 00:00:00
abstract::Transvaginal follicular aspiration (TVA) with ultrasonically guided needles allows the transfer of in vitro generated embryos to the fallopian tubes (TET), performing only one surgical procedure in the process. Up to now, this approach has been used to treat 16 couples with infertility due to severe male factor. Folli...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)60136-x
更新日期:1988-09-01 00:00:00
abstract:OBJECTIVE:To evaluate the changes of pain symptoms induced by buserelin acetate, a gonadotropin-releasing hormone agonist, in a group of patients with endometriosis. DESIGN:Thirty-five infertile patients with one or more of the following symptoms (dysmenorrhea, pelvic pain, deep dyspareunia, and endometriosis stage I ...
journal_title:Fertility and sterility
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/s0015-0282(16)55792-6
更新日期:1993-03-01 00:00:00
abstract::Sperm penetration through bovine cervical mucus was determined and correlated with the sperm characteristics in three fractions of the split ejaculate and in the whole ejaculate from 40 men of infertile couples in which a female cause of infertility had been ruled out. Sperm concentration and motility were found to be...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:
更新日期:1984-08-01 00:00:00
abstract:OBJECTIVE:To assess the capacity of human frozen-thawed ovarian follicles matured in xenografts to form metaphase II (MII) oocytes after xenotransplantation and exogenous stimulation. DESIGN:Prospective controlled animal study. SETTING:University hospital gynecology research unit. PATIENT(S):Ovarian fragments were o...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2014.01.038
更新日期:2014-05-01 00:00:00
abstract::This study compares the quality, developmental capacity and global gene expression profile of embryos generated from superovulated and natural cycles. We found that ovarian stimulation impaired quality of blastocysts (cell number: 68.9 +/- 6.9 vs. 76.8 +/- 7.9; diameter of blastocysts: 145.3 +/- 76.9 microm vs. 152.6 ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2010.03.018
更新日期:2010-05-15 00:00:00
abstract:OBJECTIVE:Our objective is to report observed changes in thyroid-stimulating hormone (TSH) in two patients undergoing super-ovulation for IVF. DESIGN:Case report. SETTING:Private assisted reproduction practices. PATIENT(S):Two hypothyroid women taking thyroxine replacement therapy undergoing super-ovulation for IVF....
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2009.11.051
更新日期:2010-05-01 00:00:00
abstract:OBJECTIVE(S):To cryopreserve micromanipulated ooplast segments (microcytoplasts) from mouse oocytes, compare microcytoplast and parent or recipient oocyte fusion performed within or without the zona pellucida, compare electrofusion of fresh or frozen oocyte with frozen-thawed microcytoplasts, and assess spindle integri...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2006.07.1529
更新日期:2007-04-01 00:00:00
abstract:OBJECTIVE:To describe the prevalence of hysteroscopic findings and histologic diagnoses in patients with abnormal uterine bleeding (AUB). DESIGN:Retrospective series of consecutive patients. SETTING:Tertiary care university hospital and private office. PATIENT(S):Four thousand fifty-four hysteroscopies with biopsy i...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2007.05.045
更新日期:2008-06-01 00:00:00
abstract:OBJECTIVE:To investigate the biocompatibility of a novel copper-containing composite to provide preclinical data for clinical application of intrauterine device (IUD) or intra-vas device (IVD). DESIGN:Prospective experimental study. SETTING:Good laboratory practices laboratories. ANIMALS:Twenty healthy adult mice (S...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2010.04.041
更新日期:2011-03-15 00:00:00
abstract::Catecholamine concentrations were determined in left spermatic vein blood of 18 patients with varicocele. These values were compared with concentrations of catecholamines in the right femoral vein. Of 18 patients studied, 6 had moderate to marked elevations of spermatic vein catecholamine concentrations. Norepinephrin...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)41541-4
更新日期:1975-12-01 00:00:00
abstract:OBJECTIVE:To investigate the possibility of genetic contribution of CYP1A1, CYP19, GSTM1, and GSTT1 polymorphisms to endometriosis. DESIGN:Genetic polymorphism analysis. SETTING:Case-control study. PATIENT(S):A group of 275 women with sporadic endometriosis was compared with a group of 346 fertile, endometriosis-fre...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(02)04817-3
更新日期:2003-03-01 00:00:00
abstract::Sperm banking with the relatively simple and economical biologic freezer (Linde BF-5; Union Carbide Corporation, Linde Division, New York, N.Y.) was compared with controlled-rate freezer (Linde CRF-1; Union Carbide), using different freezing rates and cryoprotective media. The results were assessed by comparing prefre...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:
更新日期:1981-05-01 00:00:00
abstract:OBJECTIVE:To compare blastocyst production, after IVF and ICSI, from sheep oocytes of various quality. Sham-injected oocytes and parthenogenetic activated oocyte groups were considered as control. DESIGN:Prospective experimental study. SETTING:University. ANIMAL(S):Three- to 6-month-old sheep. INTERVENTION(S):Oocyt...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2011.12.043
更新日期:2012-04-01 00:00:00
abstract::The role of insulin sensitizing agents is discussed at length in this Committee Opinion. ...
journal_title:Fertility and sterility
pub_type: 杂志文章,评审
doi:10.1016/j.fertnstert.2008.08.004
更新日期:2008-11-01 00:00:00
abstract::Seminal fluid is often viewed as simply a vehicle to carry sperm to fertilize the oocyte, but a more complex function in influencing female reproductive physiology is now evident. Remarkably, seminal fluid contains soluble and exosome-born signaling agents that interact with the female reproductive tract to prime the ...
journal_title:Fertility and sterility
pub_type: 杂志文章,评审
doi:10.1016/j.fertnstert.2016.07.1101
更新日期:2016-09-01 00:00:00
abstract::The glycolytic enzymes, lactate dehydrogenase (LDH), glucose-6-phosphate dehydrogenase, and hexokinase in cervical mucus of 96 ovulatory women were serially measured daily during the menstrual cycle. Among all of the enzymes, the cyclic changes in LDH activity were the most significant, being high during the prolifera...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)44533-4
更新日期:1980-02-01 00:00:00
abstract:OBJECTIVE:To assess whether supplementation with hCG throughout the secretory phase of hormonally modulated cycles of frozen-thawed embryos might positively affect the outcome of such cycles. DESIGN:Prospective, randomized controlled trial. SETTING:University teaching hospital. PATIENT(S):Patients undergoing frozen-...
journal_title:Fertility and sterility
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.fertnstert.2009.02.027
更新日期:2010-02-01 00:00:00
abstract::We wished to verify whether semen processing by discontinuous double-layered density gradient centrifugation could improve sperm apoptotic DNA fragmentation rates using a commercially available deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling assay in 35 consecutive men presenting for ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2007.01.059
更新日期:2008-09-01 00:00:00
abstract::A number of patients who have involuntary infertility show sperm antibodies in the blood serum, as detected by two quite different methods of spermagglutination. These techniques are the Kibrick method (gelatin agglutination test) and the F-D method (tube-slide agglutination test). By the former technique, the sera of...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)43158-4
更新日期:1978-03-01 00:00:00
abstract::The steroidogenic activity of purified theca interstitial cells (TIC) from polycystic ovaries of a patient with virilizing congenital adrenal hyperplasia due to 21-hydroxylase deficiency was studied in serum-free medium. The results show that CVAH TIC have high intrinsic basal androgen secretory activity that is drama...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)60450-8
更新日期:1989-01-01 00:00:00