Abstract:
:We have isolated a senescence-specific clone (pSEN) from a cDNA library constructed from late passage WI-38 human diploid fibroblast that accounts for approximately 1% of the recombinants. Nucleotide sequence analysis of the partial cDNA clone has led to the identification of pSEN as elongation factor I alpha. Northern analysis of poly(A)+ RNA from various intermediate population doubling levels shows that a 2.2 kb transcript hybridizes to pSEN but is expressed prior to PDL-40 at very low levels. This transcript begins to accumulate at PDL-40 and is induced approximately 50-fold just prior to senescence. Furthermore, this transcript was shown to be specific to Go of the cell cycle whereas a second, lower molecular weight transcript (1.6 kb) was observed during S phase (Giordano and Foster, unpublished data). The 2.2 kb transcript is also detected in neonatal foreskin cells but very little increase in abundance is observed between early and late passage cells. Sucrose gradient fractionation of RNA from late passage WI-38 cells suggests that the lower molecular weight transcript is associated with the polysome fraction while the 2.2 kb transcript sediments with the nonpolysomal fraction. Thus, the possibility exists that the 1.6 kb transcript is derived from the 2.2 kb transcript.
journal_name
Exp Gerontoljournal_title
Experimental gerontologyauthors
Giordano T,Kleinsek D,Foster DNdoi
10.1016/0531-5565(89)90056-9subject
Has Abstractpub_date
1989-01-01 00:00:00pages
501-13issue
5-6eissn
0531-5565issn
1873-6815journal_volume
24pub_type
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journal_title:Experimental gerontology
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journal_title:Experimental gerontology
pub_type: 杂志文章,评审
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journal_title:Experimental gerontology
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journal_title:Experimental gerontology
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pub_type: 杂志文章
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journal_title:Experimental gerontology
pub_type: 杂志文章,多中心研究
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doi:10.1016/s0531-5565(99)00026-1
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pub_type: 杂志文章
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journal_title:Experimental gerontology
pub_type: 杂志文章
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journal_title:Experimental gerontology
pub_type: 杂志文章,评审
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journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/j.exger.2006.04.007
更新日期:2006-08-01 00:00:00
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journal_title:Experimental gerontology
pub_type: 杂志文章,评审
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journal_title:Experimental gerontology
pub_type: 杂志文章,随机对照试验
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更新日期:2015-10-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/s0531-5565(02)00149-3
更新日期:2003-01-01 00:00:00
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journal_title:Experimental gerontology
pub_type: 杂志文章
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pub_type: 杂志文章,随机对照试验
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