当前位置： SCI文献检索 > CYTOKINE期刊下所有文献 > Low concentrations of recombinant granulocyte macrophage-colony stimulating factor derived from Chinese hamster ovary cells augments long-term bioactivity with delayed clearance in vitro.

Low concentrations of recombinant granulocyte macrophage-colony stimulating factor derived from Chinese hamster ovary cells augments long-term bioactivity with delayed clearance in vitro.

Abstract：

:To date, the biological activity of granulocyte macrophage-colony stimulating factor (GM-CSF) has been investigated by using mostly Escherichia coli- or yeast cell-derived recombinant human GM-CSF (erhGM-CSF and yrhGM-CSF, respectively). However, Chinese hamster ovary cell-derived recombinant human GM-CSF (crhGM-CSF), as well as natural human GM-CSF, is a distinct molecule that includes modifications by complicated oligosaccharide moieties. In the present study, we reevaluated the bioactivity of crhGM-CSF by comparing it with those of erhGM-CSF and yrhGM-CSF. The effect of short-term stimulation (0.5h) on the activation of neutrophils/monocytes or peripheral blood mononuclear cells (PBMCs) by crhGM-CSF was lower than those with erhGM-CSF or yrhGM-CSF at low concentrations (under 60pM). Intermediate-term stimulation (24h) among the different rhGM-CSFs with respect to its effect on the activation of TF-1 cells, a GM-CSF-dependent cell line, or PBMCs was not significantly different. In contrast, the proliferation/survival of TF-1 cells or PBMCs after long-term stimulation (72-168h) was higher at low concentrations of crhGM-CSF (15-30pM) than that of cells treated with other GM-CSFs. The proportion of apoptotic TF-1 cells after incubation with crhGM-CSF for 72h was lower than that of cells incubated with other rhGM-CSFs. These effects were attenuated by desialylation of crhGM-CSF. Clearance of crhGM-CSF but not desialylated-crhGM-CSF by both TF-1 cells and PBMCs was delayed compared with that of erhGM-CSF or yrhGM-CSF. These results suggest that sialylation of oligosaccharide moieties delayed the clearance of GM-CSF, thus eliciting increased long-term bioactivity in vitro.

journal_name

Cytokine

journal_title

Cytokine

authors

Hashimoto A,Tanaka T,Itoh Y,Yamagata A,Kitamura N,Tazawa R,Nakagaki K,Nakata K

doi

10.1016/j.cyto.2014.03.009

subject

Has Abstract

pub_date

2014-08-01 00:00:00

pages

118-26

issue

2

eissn

1043-4666

issn

1096-0023

pii

S1043-4666(14)00077-5

journal_volume

68

pub_type

杂志文章

相关文献

CYTOKINE文献大全
  • Increased expression of IL-32 correlates with IFN-γ, Th1 and Tc1 in virologically suppressed HIV-1-infected patients.

    abstract：:Following recent attention focused on IL-32 as an important component involved in the inflammatory cytokine network, we speculated that IL-32's action on IFN-γ and IFN-γ secreting T cell subsets may help sustain the immune activation and dysregulation found in patients with HIV-1 achieving viral suppression. To explor...

    journal_title：Cytokine

    pub_type： 杂志文章

    doi：10.1016/j.cyto.2019.01.012

    authors： Santinelli L,Statzu M,Pierangeli A,Frasca F,Bressan A,Pinacchio C,Nonne C,Turriziani O,Antonelli G,d'Ettorre G,Scagnolari C

    更新日期：2019-08-01 00:00:00

  • Tumor necrosis factor-α and interleukin-1β gene polymorphisms and risk of brain abscess in North Indian population.

    abstract：OBJECTIVE:Brain abscess develops in response to a parenchymal infection with pyogenic bacteria. Tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) are the most crucial pro-inflammatory cytokines with both beneficial and destructive properties for the central nervous system. The present study evaluated the assoc...

    journal_title：Cytokine

    pub_type： 杂志文章

    doi：10.1016/j.cyto.2015.07.009

    authors： Mishra P,Prasad KN,Singh K,Bajpai A,Sahu RN,Ojha BK

    更新日期：2015-09-01 00:00:00

  • Pretreatment of nafithromycin attenuates inflammatory response in murine lipopolysaccharide induced acute lung injury.

    abstract：:Acute respiratory distress syndrome following an acute lung injury (ALI) is a life threatening inflammatory condition predominantly characterized by vascular protein leakage, neutrophil recruitment and overexpression of proinflammatory cytokines. Pulmonary and systemic bacterial infections are the major cause of ALI w...

    journal_title：Cytokine

    pub_type： 杂志文章

    doi：10.1016/j.cyto.2020.155049

    authors： Trivedi J,Shaikh J,Chavan N,Thorve D,Chaudhary B,Karade A,Gupta S,Patel A,Bhagwat S

    更新日期：2020-05-01 00:00:00

  • Regulation of the interleukin 2 receptor complex tyrosine kinase activity in vitro.

    abstract：:Interleukin 2 (IL-2) has been shown to stimulate tyrosine phosphorylation of a number of proteins requiring only the p75 beta chain of the IL-2 receptor. Unlike the receptors for epidermal growth factor, insulin, and other growth factors, the p55-alpha and p75-beta chains of the IL-2 receptor have no tyrosine protein ...

    journal_title：Cytokine

    pub_type： 杂志文章

    doi：10.1016/1043-4666(91)90047-h

    authors： Michiel DF,Garcia GG,Evans GA,Farrar WL

    更新日期：1991-09-01 00:00:00

  • Interferon-alpha and interleukin-6 in SLE serum induce the differentiation and maturation of dendritic cells derived from CD34+ hematopoietic precursor cells.

    abstract：:The objective of this study was to investigate the effects of the cytokines IFN-alpha, IL-6 and IL-10 present in the serum of systemic lupus erythematosus (SLE) patients on the differentiation and maturation of DCs derived from CD34(+) hematopoietic precursor cells (HPCs). CD34(+) HPCs cultured in SLE serum containing...

    journal_title：Cytokine

    pub_type： 杂志文章

    doi：10.1016/j.cyto.2010.02.017

    authors： Zhang R,Xing M,Ji X,Gu L,Yang X,Wang H,Jiang P

    更新日期：2010-05-01 00:00:00

  • Pattern of cytokine expression by rat liver epithelial cells supporting long-term culture of human CD34(+)umbilical cord blood cells.

    abstract：:Fetal liver is the main site of haematopoiesis during mid-gestation. The adult liver still provides a favourable environment for extramedullary haematopoiesis. Nevertheless, regulation of liver haematopoiesis by cell-cell contacts or by cytokines remains poorly understood. Recently, we have shown that rat liver epithe...

    journal_title：Cytokine

    pub_type： 杂志文章

    doi：10.1006/cyto.1999.0639

    authors： Rialland M,Corlu A,Ilyin G,Cabillic F,Lamy I,Guguen-Guillouzo C

    更新日期：2000-07-01 00:00:00

  • Inhibition of cytokine activation processes in vitro by tenidap, a novel anti-inflammatory agent.

    abstract：:Tenidap is a novel anti-inflammatory and anti-arthritic agent that lowers intracellular pH and suppresses anion transport when applied to cells in vitro. Both of these parameters are known to influence pro-inflammatory cell function. To investigate whether tenidap can modulate cellular responses to cytokine stimulatio...

    journal_title：Cytokine

    pub_type： 杂志文章

    doi：10.1006/cyto.1995.1027

    authors： McNiff PA,Laliberte RE,Svensson L,Pazoles CJ,Gabel CA

    更新日期：1995-02-01 00:00:00

  • Expression analysis and characterization of alternatively spliced transcripts of human IL-7Ralpha chain encoding two truncated receptor proteins in relapsed childhood all.

    abstract：:In the family of cytokines and cytokine receptors, alternative splicing of pre-mRNA is a frequently observed process that generates different protein isoforms from a single genetic locus. The splicing-derived cytokine receptor protein isoforms are mostly soluble receptors or show alterations in their cytoplasmic domai...

    journal_title：Cytokine

    pub_type： 杂志文章

    doi：10.1006/cyto.2000.0777

    authors： Korte A,Köchling J,Badiali L,Eckert C,Andreae J,Geilen W,Kebelmann-Betzing C,Taube T,Wu S,Henze G,Seeger K

    更新日期：2000-11-01 00:00:00

  • Scatter factor affects major changes in the cytoskeletal organization of epithelial cells.

    abstract：:The effects of scatter factor on the cytoskeleton of MDCK and PtK2 cells are described. During the first 6 h after the addition of scatter factor, MDCK cells were found to increase their projected areas twofold, as well as the number and size of their F-actin stress fibers. In contrast PtK2 cells showed no change in t...

    journal_title：Cytokine

    pub_type： 杂志文章

    doi：10.1016/1043-4666(91)90498-3

    authors： Dowrick PG,Prescott AR,Warn RM

    更新日期：1991-07-01 00:00:00

  • Response to thalidomide in multiple myeloma: impact of angiogenic factors.

    abstract：:Thalidomide has antiangiogenic and immunomodulatory effects, mediated by several cytokines such as vascular endothelial growth factor (VEGF), fibroblastic growth factor (FGF-2), hepatocyte growth factor (HGF), interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-alpha). Although extramedullary plasmacytomas (EMP)...

    journal_title：Cytokine

    pub_type： 杂志文章

    doi：10.1016/j.cyto.2004.02.002

    authors： Rosiñol L,Cibeira MT,Segarra M,Cid MC,Filella X,Aymerich M,Rozman M,Arenillas L,Esteve J,Bladé J,Montserrat E

    更新日期：2004-05-21 00:00:00

  • Interleukin-10 controls human peripheral PMN activation triggered by lipopolysaccharide.

    abstract：:Large amounts of anti-inflammatory mediators, such as interleukin (IL)-10, are produced and found early in the course of sepsis. We explore the role of IL-10 on neutrophil (PMN) activation/function using an in vitro model. Isolated human PMN were pre-incubated with lipopolysaccharide (LPS) and/or IL-10 for 18h. Subseq...

    journal_title：Cytokine

    pub_type： 杂志文章

    doi：10.1016/j.cyto.2013.03.025

    authors： Martire-Greco D,Rodriguez-Rodrigues N,Landoni VI,Rearte B,Isturiz MA,Fernández GC

    更新日期：2013-06-01 00:00:00

  • Tumor-derived cytokines impair myogenesis and alter the skeletal muscle immune microenvironment.

    abstract：:Muscle wasting is a decline in skeletal muscle mass and function that is associated with aging, obesity, and a spectrum of pathologies including cancer. Cancer-associated wasting not only reduces quality of life, but also directly impacts cancer mortality, chemotherapeutic efficacy, and surgical outcomes. There is an ...

    journal_title：Cytokine

    pub_type： 杂志文章

    doi：10.1016/j.cyto.2017.11.006

    authors： Hogan KA,Cho DS,Arneson PC,Samani A,Palines P,Yang Y,Doles JD

    更新日期：2018-07-01 00:00:00

  • Interleukin-10 regulates arterial pressure in early primate pregnancy.

    abstract：OBJECTIVES:In pregnancy, the placental contribution of cytokines to maternal immunosuppression has been established, however their role in normal maternal blood pressure regulation has not been identified. We investigate the contribution of interleukin-10 (IL-10) and tumor necrosis factor-alpha (TNF-alpha) to the vasod...

    journal_title：Cytokine

    pub_type： 杂志文章

    doi：10.1016/j.cyto.2004.10.011

    authors： Orange S,Rasko JE,Thompson JF,Vaughan J,Olive E,Pedler M,Horvath JS,Hennessy A

    更新日期：2005-02-21 00:00:00

  • Inhibition of TGFbeta1-mediated PAI-1 induction by oltipraz through selective interruption of Smad 3 activation.

    abstract：:Transforming growth factor-beta1 (TGFbeta1) induces plasminogen activator inhibitor-1 (PAI-1) as a major target protein. PAI-1 is associated with fibrosis, thrombosis, and metabolic disorders. TGFbeta1 induces PAI-1 via phosphorylation and nuclear translocation of Smads. Oltipraz inhibits TGFbeta1 expression and also ...

    journal_title：Cytokine

    pub_type： 杂志文章

    doi：10.1016/j.cyto.2006.10.001

    authors： Cho IJ,Kim SH,Kim SG

    更新日期：2006-09-01 00:00:00

  • Molecular area involved in the in-vitro dimerization of the murine p55 IL-2 receptor.

    abstract：:In order to study structure-function relationships of the M(r) 55,000 subunit of the murine IL-2R (p55 IL-2R) by epitope mapping, we have expressed the p55 IL-2R molecule in a cell-free translation system. Under these in vitro conditions, we detected the expected p55 IL-2R polypeptide initiated at Met 1 and, in additi...

    journal_title：Cytokine

    pub_type： 杂志文章

    doi：10.1016/1043-4666(92)90066-z

    authors： Lorenzo F,Jaulin C,Jankovic DL,Theze J

    更新日期：1992-07-01 00:00:00

  • Interleukin 10 suppresses phagocytic and antihyphal activities of human neutrophils.

    abstract：:We investigated the effects of human interleukin 10 (IL-10) on the antibacterial and antifungal activities of human neutrophils (PMNs) against Staphylococcus aureus and Candida albicans. Incubation of PMNs from healthy volunteers with 20-100 ng/ml of IL-10 at 37 degrees C for 1 h suppressed phagocytosis of serum-opson...

    journal_title：Cytokine

    pub_type： 杂志文章

    doi：10.1006/cyto.1999.0567

    authors： Roilides E,Katsifa H,Tsaparidou S,Stergiopoulou T,Panteliadis C,Walsh TJ

    更新日期：2000-04-01 00:00:00

  • Immune response of Th17-associated cytokines by peripheral blood mononuclear cells from patients with chronic hepatitis C virus infection.

    abstract：:Hepatitis C virus (HCV) chronic infection causes severe cellular immune dysfunction. Here, we investigated the production of Th17-associated cytokines by peripheral blood mononuclear cells (PBMCs) of untreated patients with HCV, patients presenting an early virologic response (EVR) after 12weeks of treatment with inte...

    journal_title：Cytokine

    pub_type： 杂志文章

    doi：10.1016/j.cyto.2017.09.015

    authors： Cabral MS,Santos TPS,Santos PL,Schinoni MI,Oliveira IS,Pereira AB,Atta AM,Sousa-Atta MLB

    更新日期：2018-02-01 00:00:00

  • Serological signatures of clinical cure following successful treatment with sodium stibogluconate in Ethiopian visceral leishmaniasis.

    abstract：BACKGROUND:In Ethiopia, visceral leishmaniasis (VL) is a growing public health threat. Among the key challenges in VL control in Ethiopia is lack of an effective test of cure. The recommended test of cure is parasite detection. As sterile cure is not expected with the current widely used drugs, the value of parasite de...

    journal_title：Cytokine

    pub_type： 临床试验,杂志文章,多中心研究

    doi：10.1016/j.cyto.2016.11.016

    authors： Gadisa E,Tasew G,Abera A,Gelaye W,Chanyalew M,Abebe M,Laskay T,Aseffa A

    更新日期：2017-03-01 00:00:00

  • Is there a morning-to-evening difference in the acute IL-6 and cortisol responses to resistance exercise?

    abstract：:Exercise training is known to induce a molecular adaptation process involving inflammatory responses. However any time-of-day effect of exercise on inflammatory responses remains unknown. The aim of the present study was to investigate whether acute bouts of intense exercise performed at different times of the day wou...

    journal_title：Cytokine

    pub_type： 杂志文章

    doi：10.1016/j.cyto.2011.05.005

    authors： Pledge D,Grosset JF,Onambélé-Pearson GL

    更新日期：2011-08-01 00:00:00

  • Decreased CD4+CD8low T cells in early HIV infection are associated with rapid disease progression.

    abstract：BACKGROUND:HIV rapid progressors (RPs) present with a rapid decline of CD4+ T cells within a few years of infection. Determining the underlying mechanisms throughout this decline is important to identify prognostic biomarkers and intervention strategies. Determining the numbers of CD4+ and CD8+ T cells is essential for...

    journal_title：Cytokine

    pub_type： 杂志文章

    doi：10.1016/j.cyto.2019.154801

    authors： Ding ZD,Zheng JF,Song CB,Fu YJ,Xu JJ,Jiang YJ,Shang H,Zhang ZN

    更新日期：2020-01-01 00:00:00

  • Monitoring of IL-10 in the serum of patients with advanced ovarian cancer: results from a prospective pilot-study.

    abstract：OBJECTIVES:The fact that ovarian cancer remains confined to the peritoneal cavity even in advanced stages has allowed us to surmise that local immunosuppressive factors could be involved in the tumor biology of ovarian cancer. In this context, IL-10 can be one of the key factors. By studying the kinetics of IL-10 conce...

    journal_title：Cytokine

    pub_type： 杂志文章

    doi：10.1016/j.cyto.2008.10.019

    authors： Mustea A,Braicu EI,Koensgen D,Yuan S,Sun PM,Stamatian F,Lichtenegger W,Chen FC,Chekerov R,Sehouli J

    更新日期：2009-01-01 00:00:00

  • Single nucleotide polymorphisms (SNPs) of pro-inflammatory/anti-inflammatory and thrombotic/fibrinolytic genes in patients with acute ischemic stroke in relation to TOAST subtype.

    abstract：BACKGROUND:The genetic basis of complex diseases like ischemic stroke probably consists of several predisposing risk factors, such as genes involved in inflammation and thrombotic pathways. On this basis the aim of our study was to evaluate the role of SNPs (single nucleotide polymorphisms) of some pro-inflammatory/ant...

    journal_title：Cytokine

    pub_type： 杂志文章

    doi：10.1016/j.cyto.2012.02.012

    authors： Tuttolomondo A,Di Raimondo D,Forte GI,Casuccio A,Vaccarino L,Scola L,Pecoraro R,Serio A,Clemente G,Arnao V,Palmeri M,Misiano G,Lio D,Pinto A,Licata G

    更新日期：2012-06-01 00:00:00

  • Molecular cloning, characterization and expression analysis of an IL-21 homologue from Tetraodon nigroviridis.

    abstract：:Interleukin-21 (IL-21) is an important immune cytokine that was well characterized in human and mammals, but little is known in fish. In present study, an IL-21 homologue was cloned and well characterized from Tetraodon nigroviridis. The full-length Tetraodon IL-21 cDNA was 849bp in size, containing an open reading fr...

    journal_title：Cytokine

    pub_type： 杂志文章

    doi：10.1016/j.cyto.2006.07.010

    authors： Wang HJ,Xiang LX,Shao JZ,Jia S

    更新日期：2006-08-01 00:00:00

  • Resident joint tissues, rather than infiltrating neutrophils and monocytes, are the predominant sources of TNF-alpha in zymosan-induced arthritis.

    abstract：:Intraarticular injection of zymosan (1 mg) into the knee joints of male Wistar rats led to infiltration of neutrophils and monocytes, joint swelling and elevation of tumour necrosis factor (TNF-alpha) in joint lavage fluids. Neutrophil infiltration was maximal at 5 h after induction of arthritis but had declined by 24...

    journal_title：Cytokine

    pub_type： 杂志文章

    doi：10.1006/cyto.1996.0018

    authors： Pettipher ER,Salter ED

    更新日期：1996-02-01 00:00:00

  • Upregulation of osteopontin expression via the interaction of macrophages and fibroblasts under IL-1b stimulation.

    abstract：BACKGROUND:Fibrosis is attributed to dysregulation of tissue-remodeling. In remodeling areas, fibroblasts and macrophages actively make contact with each other. Osteopontin (OPN) is a pro-fibrotic molecule, whose expression is upregulated by interleukin (IL)-1β via secretion of its downstream cytokines, such as IL-6. H...

    journal_title：Cytokine

    pub_type： 杂志文章

    doi：10.1016/j.cyto.2018.04.025

    authors： Shimodaira T,Matsuda K,Uchibori T,Sugano M,Uehara T,Honda T

    更新日期：2018-10-01 00:00:00

  • Differential effects of transforming growth factor beta2, dexamethasone and 1,25-dihydroxyvitamin D on human bone marrow stromal cells.

    abstract：:We have studied the effects of rhTGF-beta2, dexamethanosome (dex) and 1,25(OH)2D3 on human bone marrow stromal (HBMS) cells in long-term culture. A fraction on HBMS expressed type I collagen (Col I) and osteopontin, and transient treatment (48 h) with dex increased the number of alkaline phosphatase (ALP) positive cel...

    journal_title：Cytokine

    pub_type： 杂志文章

    doi：10.1006/cyto.1997.0209

    authors： Fromigué O,Marie PJ,Lomri A

    更新日期：1997-08-01 00:00:00

  • Altered plasma levels of βC and γC chain cytokines and post-treatment modulation in tuberculous lymphadenitis.

    abstract：BACKGROUND:Alterations in β common (βC) and γ common (γC) chain cytokines have been described in pulmonary tuberculosis. However, their role in tuberculous lymphadenitis (TBL) disease has not been assessed. METHODS:Thus, in the present study, we have examined the systemic levels of βC and γC chain cytokines in TBL, la...

    journal_title：Cytokine

    pub_type： 杂志文章

    doi：10.1016/j.cyto.2020.155405

    authors： Kathamuthu GR,Moideen K,Sridhar R,Baskaran D,Babu S

    更新日期：2021-02-01 00:00:00

  • IL-17/Th17 targeting: on the road to prevent chronic destructive arthritis?

    abstract：:Interleukin-17A (IL-17A) contributes to the pathogenesis of arthritis. Data from experimental arthritis indicate IL-17 receptor signaling as a critical pathway in turning an acute synovitis into a chronic destructive arthritis. The identification of six IL-17 family members (IL-17A-F) may extend the role of this novel...

    journal_title：Cytokine

    pub_type： 杂志文章,评审

    doi：10.1016/j.cyto.2007.09.014

    authors： Lubberts E

    更新日期：2008-02-01 00:00:00

  • Regulation of free and bound leptin and soluble leptin receptors during inflammation in mice.

    abstract：:Leptin, an appetite-regulating hormone/cytokine, circulates both free and bound to soluble leptin receptors (s-leptin R). An electrochemiluminescence (ECL) assay for the quantitative measurement of murine s-leptin R was developed. The absence of s-leptin R immunoreactivity in the serum of db(pas)/db(pas)mice demonstra...

    journal_title：Cytokine

    pub_type： 杂志文章

    doi：10.1006/cyto.2001.0859

    authors： Voegeling S,Fantuzzi G

    更新日期：2001-04-21 00:00:00

  • Vitamin D(3) down-regulates proinflammatory cytokine response to Mycobacterium tuberculosis through pattern recognition receptors while inducing protective cathelicidin production.

    abstract：:A well-known association between vitamin D(3) and infection with Mycobacterium tuberculosis has previously been reported, but little is known regarding the underlying mechanisms. We have investigated how 1,25-dihydroxyvitamin D(3) [1,25(OH)(2)D(3)] affects the proinflammatory cytokine production induced by M. tubercul...

    journal_title：Cytokine

    pub_type： 杂志文章

    doi：10.1016/j.cyto.2011.04.016

    authors： Khoo AL,Chai LY,Koenen HJ,Oosting M,Steinmeyer A,Zuegel U,Joosten I,Netea MG,van der Ven AJ

    更新日期：2011-08-01 00:00:00