Abstract:
:Following a radiation incident, preliminary dose estimates made by γ-H2AX foci analysis can supplement the early triage of casualties based on clinical symptoms. Sample processing time is important when many individuals need to be rapidly assessed. A protocol was therefore developed for high sample throughput that requires less than 0.1 ml blood, thus potentially enabling finger prick sampling. The technique combines red blood cell lysis and leukocyte fixation in one step on a 96 well plate, in contrast to the routine protocol, where lymphocytes in larger blood volumes are typically separated by Ficoll density gradient centrifugation with subsequent washing and fixation steps. The rapid '96 well lyse/fix' method reduced the estimated sample processing time for 96 samples to about 4 h compared to 15 h using the routine protocol. However, scoring 20 cells in 96 samples prepared by the rapid protocol took longer than for the routine method (3.1 versus 1.5 h at zero dose; 7.0 versus 6.1 h for irradiated samples). Similar foci yields were scored for both protocols and consistent dose estimates were obtained for samples exposed to 0, 0.2, 0.6, 1.1, 1.2, 2.1 and 4.3 Gy of 250 kVp X-rays at 0.5 Gy/min and incubated for 2 h. Linear regression coefficients were 0.87 ± 0.06 (R (2) = 97.6%) and 0.85 ± 0.05 (R (2) = 98.3%) for estimated versus actual doses for the routine and lyse/fix method, respectively. The lyse/fix protocol can therefore facilitate high throughput processing for γ-H2AX biodosimetry for use in large scale radiation incidents, at the cost of somewhat longer foci scoring times.
journal_name
PeerJjournal_title
PeerJauthors
Moquet J,Barnard S,Rothkamm Kdoi
10.7717/peerj.282subject
Has Abstractpub_date
2014-03-06 00:00:00pages
e282issn
2167-8359pii
282journal_volume
2pub_type
杂志文章相关文献
PeerJ文献大全abstract:PURPOSE:The aim of this study was to evaluate the effects of low-volume high-intensity interval training and continuous low to moderate intensity training on quality of life, functional capacity and cardiovascular disease risk factors in cancer survivors. METHODS:Cancer survivors within 24 months post-diagnosis were r...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.2613
更新日期:2016-10-20 00:00:00
abstract::Because of the environmental sensitivity of the Black Sea, as a semi-enclosed sea, any subsea oil spill can cause destructive impacts on the marine environment and beaches. Employing numerical modeling as a prediction tool is one of the most efficient methods to understand oil spill behavior under various environmenta...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.5448
更新日期:2018-08-29 00:00:00
abstract::The rapidly changing climate makes humans realize that there is a critical need to incorporate climate change adaptation into conservation planning. Whether the wintering habitats of Great Bustards (Otis tarda dybowskii), a globally endangered migratory subspecies whose population is approximately 1,500-2,200 individu...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.1630
更新日期:2016-02-01 00:00:00
abstract::Background. Eucryptodiran turtles from the Late Jurassic (mainly Kimmeridgian) deposits of the Jura Mountains (Switzerland and France) are among the earliest named species traditionally referred to the Plesiochelyidae, Thalassemydidae, and Eurysternidae. As such, they are a reference for the study of Late Jurassic euc...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.369
更新日期:2014-05-13 00:00:00
abstract::The structural and material properties of insect cuticle remain largely unexplored, even though they comprise the majority (approximately 80%) of animals. Insect cuticle serves many functions, including protection against predatory attacks, which is especially beneficial to species failing to employ effective running ...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.501
更新日期:2014-07-31 00:00:00
abstract:OBJECTIVE:To determine the profile of 14 polybrominated diphenyl ethers (PBDEs) and 23 polychlorinated biphenyls (PCBs) in serum of domestic canines and whether this was predictive of thyroid hormone status. SAMPLES:Serum samples were collected from 51 client-owned dogs visiting the University of California Davis Will...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.3780
更新日期:2017-09-12 00:00:00
abstract::Seabed mapping can quantify the extent of benthic habitats that comprise marine ecosystems, and assess the impact of fisheries on an ecosystem. In this study, the distribution of seabed habitats in a proposed no-take Marine Reserve along the northeast coast of Great Barrier Island, New Zealand, was mapped using underw...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.1446
更新日期:2015-12-10 00:00:00
abstract::Sea cucumbers possess the remarkable capacity to regenerate their body parts or organs. Regeneration of host organs and/or body parts involves reconstruction of the host associated microbiota, however, the dynamics and contribution of microbiota to the regeneration process are largely unknown due to a lack of experime...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.10260
更新日期:2020-12-01 00:00:00
abstract::The effects of age on performance of life-history traits are diverse, but a common outcome is senescence, an irreversible deterioration of physical and physiological capabilities of older individuals. Immune response is potentially bound to senescence. However, little is known about immune response ageing in amphibian...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.6104
更新日期:2018-12-20 00:00:00
abstract::Bacterial populations present in Hg-rich environments have evolved biological mechanisms to detoxify methylmercury and other organometallic mercury compounds. The most common resistance mechanism relies on the H(+)-assisted cleavage of the Hg-C bond of methylmercury by the organomercurial lyase MerB. Although the init...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.1127
更新日期:2015-07-28 00:00:00
abstract::Background. Quotations and references are an indispensable element of scientific communication. They should support what authors claim or provide important background information for readers. Studies indicate, however, that quotations not serving their purpose-quotation errors-may be prevalent. Methods. We carried out...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.1364
更新日期:2015-10-27 00:00:00
abstract::Relative gene expression data obtained from quantitative RT-PCR (RT-qPCR) experiments are dependent on appropriate normalization to represent true values. It is common to use constitutively expressed endogenous reference genes (RGs) for normalization, but for this strategy to be valid the RGs must be stably expressed ...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.7181
更新日期:2019-06-26 00:00:00
abstract::Harmine is the β-carboline alkaloid with the highest concentration in the psychotropic plant decoction Ayahuasca. In rodents, classical antidepressants reverse the symptoms of depression by stimulating neuronal proliferation. It has been shown that Ayahuasca presents antidepressant effects in patients with depressive ...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.2727
更新日期:2016-12-06 00:00:00
abstract::Pseudoterranovosis is a well-known human disease caused by anisakid larvae belonging to the genus Pseudoterranova. Human infection occurs after consuming infected fish. Hence the presence of Pseudoterranova larvae in the flesh of the fish can cause serious losses and problems for the seafood, fishing and fisheries ind...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.1722
更新日期:2016-03-15 00:00:00
abstract::Quantification of fine-scale movement, performance, and energetics of hunting by large carnivores is critical for understanding the physiological underpinnings of trophic interactions. This is particularly challenging for wide-ranging terrestrial canid and felid predators, which can each affect ecosystem structure thr...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.3701
更新日期:2017-08-17 00:00:00
abstract::Background. Blacklegged ticks (Ixodes scapularis) are important disease vectors in the United States, known to transmit a variety of pathogens to humans, including bacteria, protozoa, and viruses. Their importance as a disease vector necessitates reliable and comparable methods for extracting microbial DNA from ticks....
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.1147
更新日期:2015-08-13 00:00:00
abstract::The Asian clam, Corbicula fluminea, is a commonly consumed small freshwater bivalve in East Asia. However, available genetic information of this clam is still limited. In this study, the transcriptome of female C. fluminea was sequenced using the Illumina HiSeq 2500 platform. A total of 89,563 unigenes were assembled ...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.7770
更新日期:2019-10-22 00:00:00
abstract::We developed a novel method based on the Fourier analysis of protein molecular surfaces to speed up the analysis of the vast structural data generated in the post-genomic era. This method computes the power spectrum of surfaces of the molecular electrostatic potential, whose three-dimensional coordinates have been eit...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.185
更新日期:2013-10-22 00:00:00
abstract::Background/Aim. Exercise designed to condition the lumbar extensor musculature is often included in resistance training (RT) programs. It is suggested that deconditioning of this musculature may be linked to low back pain. Thus effective means of conditioning these muscles are of interest to pursue. Evidence suggests ...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.878
更新日期:2015-03-31 00:00:00
abstract::Background. Caesarean birth rates in North America continue to rise, in the absence of benefit for mothers and babies. One reason may be that hospitalized labouring women spend most of their labours in recumbent or semi-recumbent positions. Although hands-and-knees position has theoretical advantages, efforts to encou...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.25
更新日期:2013-02-12 00:00:00
abstract::Local ancestry estimation infers the regional ancestral origin of chromosomal segments in admixed populations using reference populations and a variety of statistical models. Integrating local ancestry into complex trait genetics has the potential to increase detection of genetic associations and improve genetic predi...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.10090
更新日期:2020-10-02 00:00:00
abstract::In this paper, we map the distribution of scale-rayed wrasse Acantholabrus palloni in eastern Skagerrak based on a combination of verified and personally communicated angling records. Long thought to be occasional vagrants outside its known range in the eastern Atlantic Ocean and Mediterranean Sea, we ask if this rare...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.5900
更新日期:2018-11-06 00:00:00
abstract:Background:Influenza A (H5N1) virus is a global concern with potential as a pandemic threat. High sequence variability of influenza A viruses is a major challenge for effective vaccine design. A continuing goal towards this is a greater understanding of influenza A (H5N1) proteome sequence diversity in the context of t...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.7954
更新日期:2020-05-27 00:00:00
abstract::3D printers that build objects using extruded thermoplastic are quickly becoming commonplace tools in laboratories. We demonstrate that with appropriate handling, these devices are capable of producing sterile components from a non-sterile feedstock of thermoplastic without any treatment after fabrication. The fabrica...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.2661
更新日期:2016-12-01 00:00:00
abstract::The systematic relationships of taxa traditionally referred to as 'basal ornithopods' or 'hypsilophodontids' remain poorly resolved since it was discovered that these taxa are not a monophyletic group, but rather a paraphyletic set of neornithischian taxa. Thus, even as the known diversity of these taxa has dramatical...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.1523
更新日期:2015-12-22 00:00:00
abstract::The structural complexity of coral reefs plays a major role in the biodiversity, productivity, and overall functionality of reef ecosystems. Conventional metrics with 2-dimensional properties are inadequate for characterization of reef structural complexity. A 3-dimensional (3D) approach can better quantify topography...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.1077
更新日期:2015-07-07 00:00:00
abstract::Increasing cross-border trade of medicinal and aromatic plants (MAPs) has put heavy pressure on a considerable number of species in the Himalayas. One of the threatened species in Nepal is Aconitum spicatum. Unfortunately for this species and for many others, our knowledge on population ecology and performance across ...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.7574
更新日期:2019-09-10 00:00:00
abstract::Preserved labile tissues (e.g., skin, muscle) in the fossil record of terrestrial vertebrates are increasingly becoming recognized as an important source of biological and taphonomic information. Here, we combine a variety of synchrotron radiation techniques with scanning electron and optical microscopy to elucidate t...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.7875
更新日期:2019-10-16 00:00:00
abstract::Knowledge on the precise identification of fish resources is critical for sustainable fisheries management. This study employs the DNA barcoding approach to generate a molecular taxonomic catalogue of commercially important reef fishes in the waters of Weh Island (Aceh Province), the most northerly inhabited island in...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.9641
更新日期:2020-08-05 00:00:00
abstract::Plasmid vectors based on bacteriophage integrases are important tools in molecular microbiology for the introduction of foreign DNA, especially into bacterial species where other systems for genetic manipulation are limited. Site specific integrases catalyze recombination between phage and bacterial attachment sites (...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.4784
更新日期:2018-05-04 00:00:00