Interactions between Epac1 and ezrin in the control of endothelial barrier function.

abstract：:Glyoxalase- and methylglyoxal-related research has required the development of quantitative and reliable techniques for the measurement of methylglyoxal-derived glycation adducts of protein and DNA. There are also other glycation adducts, oxidation adducts and nitration adducts of proteins and oxidation adducts of DNA...

journal_title：Biochemical Society transactions

authors： Rabbani N,Shaheen F,Anwar A,Masania J,Thornalley PJ

更新日期：2014-04-01 00:00:00

abstract：:TRP (transient receptor potential) channels comprise a superfamily of non-selective cation channels with at least seven subfamilies. The variety of subfamilies corresponds to the differences in the activation mechanisms and functions. TRPM3 (TRP melastatin 3) and TRPV4 (TRP vanilloid 3) have been characterized as cati...

journal_title：Biochemical Society transactions

authors： Harteneck C,Reiter B

更新日期：2007-02-01 00:00:00

abstract：:The Ets family of eukaryotic transcription factors is based around the conserved Ets DNA-binding domain. Although their DNA-binding selectivity is biochemically and structurally well characterized, structures of homodimeric and ternary complexes point to Ets domains functioning as versatile protein-interaction modules...

journal_title：Biochemical Society transactions

authors： Cooper CD,Newman JA,Gileadi O

更新日期：2014-02-01 00:00:00

abstract：:After the discovery of leucine-rich repeat kinase 2 (LRRK2) as a risk factor for sporadic Parkinson's disease (PD) and mutations in LRRK2 as a cause of some forms of familial PD, there has been substantial interest in finding chemical modulators of LRRK2 function. Most of the pathogenic mutations in LRRK2 are within t...

journal_title：Biochemical Society transactions

authors： Mortiboys H

更新日期：2016-12-15 00:00:00

abstract：:The action of VEGF (vascular endothelial growth factor) is essential to maintain proper endothelial and vascular function. VEGF stimulates virtually all aspects of endothelial function, namely proliferation, migration, permeability and nitric oxide production and release. In addition, the action of VEGF makes the endo...

journal_title：Biochemical Society transactions

authors： Waltenberger J

更新日期：2009-12-01 00:00:00

abstract：:From a protein structural viewpoint, tryptophan is often considered an inert structural amino acid, playing a role as a hydrophobic anchor in membrane proteins or as part of the hydrophobic core of soluble proteins. However, tryptophan is the only polyaromatic amino acid and, from a chemical viewpoint, possesses uniqu...

journal_title：Biochemical Society transactions

authors： Naismith JH

更新日期：2012-06-01 00:00:00

abstract：:The glutamate/aspartate transporter GLAST-1 is expressed in bone in vivo and also exists as a splice variant (GLAST-1a) in which exon 3 is excluded. Since GLAST-1 expression is regulated in bone in response to osteogenic mechanical stimuli in vivo and binding of glutamate to receptors on osteoblasts increases osteobla...

journal_title：Biochemical Society transactions

authors： Huggett JF,Mustafa A,O'neal L,Mason DJ

更新日期：2002-11-01 00:00:00

abstract：:Delineating the molecular pathways underlying seizure-induced neuronal death may yield novel strategies for brain protection against prolonged or repetitive seizures. Glutamate-mediated excitotoxicity and necrosis is a primary contributing mechanism but seizures also activate programmed (apoptotic) cell death pathways...

journal_title：Biochemical Society transactions

authors： Henshall DC

更新日期：2007-04-01 00:00:00

abstract：:Molecular, biochemical and genetic characterization of ornithine decarboxylase, S -adenosylmethionine decarboxylase and spermidine synthase establishes that these polyamine-biosynthetic enzymes are essential for growth and survival of the agents that cause African sleeping sickness, Chagas' disease, leishmaniasis and ...

journal_title：Biochemical Society transactions

authors： Heby O,Roberts SC,Ullman B

更新日期：2003-04-01 00:00:00

abstract：:ATP-binding cassette (ABC) transporters play crucial roles in cellular processes, such as nutrient uptake, drug resistance, cell-volume regulation and others. Despite their importance, all proposed molecular models for transport are based on indirect evidence, i.e. functional interpretation of static crystal structure...

journal_title：Biochemical Society transactions

authors： Husada F,Gouridis G,Vietrov R,Schuurman-Wolters GK,Ploetz E,de Boer M,Poolman B,Cordes T

更新日期：2015-10-01 00:00:00

abstract：:PET (pre-eclamptic toxaemia), characterized by pregnancy-related hypertension and proteinuria, due to widespread endothelial dysfunction, is a primary cause of maternal morbidity. Altered circulating factors, particularly the VEGF (vascular endothelial growth factor) family of proteins and their receptors, are thought...

journal_title：Biochemical Society transactions

authors： Bates DO

更新日期：2011-12-01 00:00:00

abstract：:Whereas spontaneous point mutation operates on nucleotides individually, sexual recombination manipulates the set of nucleotides within an allele as an essentially particulate unit. In principle, these two different scales of variation enable selection to follow fitness gradients in two different spaces: in nucleotide...

journal_title：Biochemical Society transactions

authors： Watson RA,Weinreich DM,Wakeley J

更新日期：2006-08-01 00:00:00

abstract：:This review focuses on recent insights into the mechanisms and the biological functions of the proteasome. This large ATP-dependent proteolytic complex is the main site for protein degradation in mammalian cells and catalyses the rapid degradation of ubiquitinated proteins, and is the source of most antigenic peptides...

journal_title：Biochemical Society transactions

authors： Goldberg AL

更新日期：2007-02-01 00:00:00

abstract：:We have identified two basic residues that are important for the recognition of secretin and vasoactive intestinal peptide (VIP) by their respective receptors. These two peptides containing an Asp residue at position 3 interacted with an arginine residue in transmembrane helix 2 (TM2) of the receptor, and the lysine r...

journal_title：Biochemical Society transactions

authors： Waelbroeck M,Perret J,Vertongen P,Van Craenenbroeck M,Robberecht P

更新日期：2002-08-01 00:00:00

abstract：:Metabolic and epidemiologic studies support the idea that the type of dietary fat is more important than the total amount of fat with respect to the development of atherosclerosis and the risk of cardiovascular heart disease. Dietary fat is carried in CMs (chylomicrons), which can be taken up by macrophages without ne...

journal_title：Biochemical Society transactions

authors： Cabello-Moruno R,Perona JS,Ruiz-Gutierrez V

更新日期：2007-06-01 00:00:00

abstract：:Allostery is a biological phenomenon of critical importance in metabolic regulation and cell signalling. The fundamental premise of classical models that describe allostery is that structure mediates 'action at a distance'. Recently, this paradigm has been challenged by the enrichment of IDPs (intrinsically disordered...

journal_title：Biochemical Society transactions

authors： Motlagh HN,Li J,Thompson EB,Hilser VJ

更新日期：2012-10-01 00:00:00

abstract：:[Ca(2+)](i) (intracellular Ca(2+) concentration) oscillations play a central role in the activation of T-lymphocytes by antigen. Oscillations in T-cells are absolutely dependent on Ca(2+) influx through store-operated CRAC channels (Ca(2+)-release-activated Ca(2+) channels), and evidence suggests that they arise from ...

journal_title：Biochemical Society transactions

authors： Lewis RS

更新日期：2003-10-01 00:00:00

abstract：:Biochemical, biophysical and biological studies of oligonucleotides containing lesions at defined sites provide a molecular basis for the effects of DNA lesions. dG (deoxyguanosine) is the most easily oxidized of the four native nucleotides. The chemical reactivity of dG correlates with compilations of mutations, whic...

journal_title：Biochemical Society transactions

authors： Greenberg MM

更新日期：2004-02-01 00:00:00

abstract：:The fission yeast Schizosaccharomyces pombe primarily detects glucose via a cAMP-signalling pathway. Components of this pathway include the Git3 G-protein-coupled receptor and a heterotrimeric G-protein, from which the Gpa2 Galpha subunit activates adenylate cyclase (Git2/Cyr1). Three additional proteins, Git1, Git7 a...

journal_title：Biochemical Society transactions

authors： Hoffman CS

更新日期：2005-02-01 00:00:00

abstract：:A common phenotypic change in cancer is a dramatic transformation of cellular glycosylation. Functional studies of particular tumour-associated oligosaccharides are difficult to interpret conclusively, but carbohydrate-binding proteins are likely to contribute to progression of the tumour. This review discusses the po...

journal_title：Biochemical Society transactions

authors： Saeland E,van Kooyk Y

更新日期：2011-01-01 00:00:00

abstract：:Unr (upstream of N-ras) is a eukaryotic RNA-binding protein that has a number of roles in the post-transcriptional regulation of gene expression. Originally identified as an activator of internal initiation of picornavirus translation, it has since been shown to act as an activator and inhibitor of cellular translatio...

journal_title：Biochemical Society transactions

authors： Ray S,Catnaigh PÓ,Anderson EC

更新日期：2015-06-01 00:00:00

abstract：:A general strategy is described for the amplified expression, purification and characterization in Escherichia coli of multidrug efflux proteins from Staphylococcus aureus, Bacillus subtilis, Methanococcus janaschii and E. coli. They all catalyse drug/H(+) antiport of substrates such as quinolones and ethidium and exe...

journal_title：Biochemical Society transactions

authors： Henderson PJ,Hoyle CK,Ward A

更新日期：2000-01-01 00:00:00

abstract：:Reactive oxygen species (ROS) were originally thought to only be released by phagocytic cells during their role in host defence. It is now clear that ROS have a cell signalling role in many biological systems, both in animals and in plants. ROS induce programmed cell death or necrosis, induce or suppress the expressio...

journal_title：Biochemical Society transactions

authors： Hancock JT,Desikan R,Neill SJ

更新日期：2001-05-01 00:00:00

abstract：:The revolution of genomics and growth of systems biology urged the creation of synthetic biology, an engineering discipline aiming at recreating and reprogramming cellular functions for industrial needs. There has been a huge effort in synthetic biology to develop versatile and programmable genetic regulators that wou...

journal_title：Biochemical Society transactions

authors： Apura P,Domingues S,Viegas SC,Arraiano CM

更新日期：2019-10-31 00:00:00

abstract：:Findings from gene expression profiling studies are leading to new diagnostic and therapeutic strategies that can be applied in medical practice, especially in the field of oncology. Promising results of gene expression profiling of the peripheral blood in patients with ischaemic stroke have been obtained in recent pi...

journal_title：Biochemical Society transactions

authors： Baird AE

更新日期：2006-12-01 00:00:00

abstract：:The control of the intracellular localization of NCS (neuronal calcium sensor) proteins is of importance for their ability to respond appropriately to differing calcium signals. We examine the localization of three NCS proteins: NCS-1, KChIP-1 (potassium-channel-interacting protein 1) and hippocalcin. Additionally, th...

journal_title：Biochemical Society transactions

authors： O'Callaghan DW,Burgoyne RD

更新日期：2003-10-01 00:00:00

abstract：:RNase A (1 mM) was incubated with glucose (0.4 M) at 37 degrees C for up to 14 days in phosphate buffer (0.2 M, pH 7.4), digested with trypsin and analysed by LC-MS. The major sites of fructoselysine formation were Lys(1), Lys(7), Lys(37) and Lys(41). Three of these sites (Lys(7), Lys(37) and Lys(41)) were also the ma...

journal_title：Biochemical Society transactions

authors： Cotham WE,Hinton DJ,Metz TO,Brock JW,Thorpe SR,Baynes JW,Ames JM

更新日期：2003-12-01 00:00:00

abstract：:Abnormal protein aggregation and intracellular or extracellular accumulation of misfolded and aggregated proteins are key events in the pathogenesis of different neurodegenerative diseases. Furthermore, endoplasmic reticulum stress and impairment of the ubiquitin-proteasome system probably contribute to neurodegenerat...

journal_title：Biochemical Society transactions

authors： Haapasalo A,Viswanathan J,Bertram L,Soininen H,Tanzi RE,Hiltunen M

更新日期：2010-02-01 00:00:00

abstract：:The process of tRNA splicing entails removal of an intron by TSEN (tRNA-splicing endonuclease) and ligation of the resulting exon halves to generate functional tRNAs. In mammalian cells, the RNA kinase CLP1 (cleavage and polyadenylation factor I subunit) associates with TSEN and phosphorylates the 3' exon at the 5' en...

journal_title：Biochemical Society transactions

authors： Mair B,Popow J,Mechtler K,Weitzer S,Martinez J

更新日期：2013-08-01 00:00:00

abstract：:The endoplasmic reticulum is traditionally perceived as the key compartment for regulating intracellular cholesterol metabolism. Increasing evidence suggests that the endocytic pathway provides an additional regulatory level governing intracellular cholesterol trafficking and homoeostasis. Sterols can enter, and appar...

journal_title：Biochemical Society transactions

authors： Hölttä-Vuori M,Ikonen E

更新日期：2006-06-01 00:00:00