Abstract:
:Activation of spinal astrocytes may contribute to neuropathic pain. Adjacent astrocytes can make direct communication through gap junctions formed by connexin 43 (Cx43) in the central nervous system. Yet, the role of spinal astroglial gap junctions in neuropathic pain is not fully understood. Since Cx43 is the connexin isoform expressed preferentially in astrocytes in the spinal cord, we used a small interfering RNA (siRNA) approach to examine whether suppression of spinal Cx43 expression inhibits mechanical hypersensitivity in rats after an L5 spinal nerve ligation (SNL). SNL rats were administered intrathecal Cx43 siRNA (3μg/15μl, twice/day) or an equal amount of mismatch siRNA (control) on days 14-17 post-SNL. Cx43 siRNA, but not mismatch siRNA, alleviated mechanical hypersensitivity in SNL rats. Furthermore, Western blot analysis showed that the pain inhibition induced by Cx43 siRNA correlated with downregulation of Cx43 expression, but not that of Cx36 (the neuronal gap junction protein) or glial fibrillary acidic protein (GFAP, a marker for reactive astrocytes) in the spinal cord of SNL rats. Western blot analysis and immunohistochemistry also showed that SNL increased GFAP expression, but decreased Cx43 expression, in spinal cord. Our results provide direct evidence that selective suppression of spinal Cx43 after nerve injury alleviates neuropathic mechanical hypersensitivity. These findings suggest that in the spinal cord, the enhanced function of astroglial gap junctions, especially those formed by Cx43, may be important to neuropathic pain in SNL rats.
journal_name
Neurosci Lettjournal_title
Neuroscience lettersauthors
Xu Q,Cheong YK,He SQ,Tiwari V,Liu J,Wang Y,Raja SN,Li J,Guan Y,Li Wdoi
10.1016/j.neulet.2014.03.004subject
Has Abstractpub_date
2014-04-30 00:00:00pages
194-199eissn
0304-3940issn
1872-7972pii
S0304-3940(14)00193-1journal_volume
566pub_type
杂志文章abstract::This study examined the effects of exposure to low concentrations (0.1-100 nM) of bisphenol A (BPA) or nonylphenol (NP) on neuronal differentiation in pheochromocytoma PC12 cells. Pre-exposure to BPA for a week or a month, but not for a day, decreased neuronal differentiation in PC12 cells. Likewise, one week's pre-ex...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2014.04.014
更新日期:2014-06-06 00:00:00
abstract::The pineal gland is innervated by pinealopetal peptidergic fibers originating in the hypothalamic nuclei which release arginine vasopressin (AVP) and arginine vasotocin (AVT) from their endings. Since the mechanism of AVT action on the pineal signal transduction and melatonin synthesis has not been determined so far, ...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(99)00066-x
更新日期:1999-03-05 00:00:00
abstract::Intra- and extracellular recordings from the in vitro rat hippocampal slice preparation have been used to investigate the influence of serotoninergic, adrenergic and cholinergic receptor antagonists on the excitability of CA1 pyramidal neurones. The serotonin receptor antagonist 4-amino-N-(1-azabicyclo[2.2.2]oct-3yl)-...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(95)12063-a
更新日期:1995-10-27 00:00:00
abstract::Seizures in genetically epilepsy-prone rats (GEPRs) may result from hypoactivity of locus coeruleus (LC) neurons during seizures. This study examined Fos-like-immunoreactivity (FLI) in the LC following audiogenic seizures in two strains of GEPRs (GEPR-9s and -3s), and following pentylenetetrazol (PTZ) or maximal elect...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(97)00611-3
更新日期:1997-09-12 00:00:00
abstract::Human monocytes and a variety of tissue macrophages, including microglia, were studied immunohistochemically to determine the expression of a novel microglial marker, human glucose transporter 5 (hGLUT5), in these cells. The hGLUT5 was not expressed in most peripheral macrophages in the normal state, but weakly expres...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(02)01332-0
更新日期:2003-02-20 00:00:00
abstract::The present study examined the coordination dynamics of the head and center of mass (COM) using accelerometry in quiet 1 and 2 leg stance with and without vision. The root mean square jerk of effectors was greater in 1 leg stance and without vision, and was greater for the head in 2 leg stance and greater at the COM f...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2013.05.068
更新日期:2013-08-26 00:00:00
abstract::The presence of the neuron specific enolase (NSE) has been tested by sandwich enzyme immunoassay in cerebrospinal fluid (CSF) of rats after intrastriatal injection of various amounts of kainic acid (KA). Incremental release of NSE was observed in CSF for increasing concentrations of injected KA. Still, a significant d...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(84)90090-9
更新日期:1984-03-23 00:00:00
abstract::The administration of DN-1417, a synthetic derivative of TRH with more potent central action, significantly reduced specific [3H]GABA binding in the cerebellum, whereas [3H]GABA binding in the cerebral cortex was not changed. Scatchard analysis showed that the decreased [3H]GABA binding in the cerebellum was due to de...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(83)90398-1
更新日期:1983-12-02 00:00:00
abstract::Focal ischemia was induced in the fronto-parietal region of rat brain, by injection of Rose Bengal, followed by light activation. Focal ischemia was accompanied by formation of PGD(2) peaking 60-90 min post irradiation and declining thereafter. Increased Cycloxygenase-2 (COX-2) expression was also observed. Control is...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(01)01675-5
更新日期:2001-05-04 00:00:00
abstract::The effects of bilateral cut of the stria terminalis (ST) on the dosage of estradiol benzoate (EB) needed to induce the lordosis reflex in the ovariectomized female rat were examined. Seven different doses ranging 0.5-20 micrograms were given s.c. in oil, daily for 2 days in a counterbalanced order, at 3-week interval...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(94)11173-g
更新日期:1995-01-23 00:00:00
abstract::Restoring corticospinal function after spinal cord injury is a significant challenge as the corticospinal tract elicits no substantive, spontaneous regeneration, and its interruption leaves a permanent deficit. The corticospinal circuit serves multiple motor and sensory functions within the mammalian nervous system as...
journal_title:Neuroscience letters
pub_type: 杂志文章,评审
doi:10.1016/j.neulet.2016.12.003
更新日期:2017-06-23 00:00:00
abstract::Generation of free radicals and subsequent lipid peroxidation have been proposed to contribute to delayed tissue damage following traumatic spinal cord injury (SCI). Ubiquinols (reduced coenzyme Q), ascorbate (vitamin C), and alpha-tocopherol (vitamin E) are endogenous antioxidants; decreases in tissue levels of these...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(90)90731-n
更新日期:1990-01-01 00:00:00
abstract::Mounting evidence supports the association between a polymorphism in the serotonin transporter gene promoter region (5-HTTLPR) and suicidal behaviour. Recently, a novel variant of the 5-HTTLPR L allele was identified. The previously unknown L(G) allele produced similar levels of gene expression to the S allele and mig...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2011.09.036
更新日期:2011-10-31 00:00:00
abstract::AMPA receptor (AMPAR) trafficking has emerged as a fundamental concept for understanding mechanisms of learning and memory as well as many neurological disorders. Classical conditioning is a simple and highly conserved form of associative learning. Our studies use an ex vivo brainstem preparation in which to study cel...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2017.02.041
更新日期:2017-04-03 00:00:00
abstract::Spirulina maxima is a blue-green micro alga that contains abundant amounts of proteins (60-70%), vitamins, chlorophyll a, and C-phycocyanin (C-PC). It has been shown to reduce oxidative stress, and prevent diabetes and non-alcoholic fatty liver disease. However, it is unclear whether Spirulina maxima 70% ethanol extra...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2018.02.057
更新日期:2018-04-23 00:00:00
abstract::Stereotaxic microinjection of herpes simplex virus (HSV) into the mouse olfactory bulb resulted in infection of neurons of the piriform cortex. Neurons infected with the wildtype HSV showed no evident phosphorylation of c-Jun N-terminal protein kinase (JNK)/c-Jun. In contrast, neurons infected with a US3 gene-disrupte...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2003.08.033
更新日期:2003-11-20 00:00:00
abstract::The actions of acridinic acid (2,3-quinoline dicarboxylic acid), a new derivative of quinolinic acid, as an antagonist of amino acid-induced excitations are described. Acridinate, like kynurenate, in the cerebral cortex reduced the effects of all amino acids equally, but in the spinal cord was significantly less activ...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(86)90173-4
更新日期:1986-05-06 00:00:00
abstract::In order to investigate whether or not nitric oxide (NO) formation underlies the cellular mechanisms of ischemic brain damage, we examined the effects of N omega-nitro-L-arginine (L-NNA), a NO synthase inhibitor, on ischemic brain edema and subsequent infarction in rats with middle cerebral artery occlusion (MCAo). Fo...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(92)90584-t
更新日期:1992-12-07 00:00:00
abstract::Chronic stress is well known to exacerbate pain. We tested the hypothesis that neonatal handling, which induces resilience to the negative impact of stress by increasing the quality and quantity of maternal care, attenuates the mechanical hyperalgesia produced by water-avoidance stress in the adult rat. Neonatal male ...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2015.01.066
更新日期:2015-03-30 00:00:00
abstract::Long-term potentiation (LTP) of perforant-path dentate granule cell synapses, in awake rats, was followed by a time-dependent expression of brain-derived neurotrophic factor (BDNF) mRNA in dentate granule cells. This BDNF expression was blocked by the N-methyl-D-aspartate (NMDA) antagonist dizocilpine maleate (MK-801)...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(93)90420-p
更新日期:1993-10-01 00:00:00
abstract::The synaptic relationships between gamma-aminobutyric acid (GABA)-immunoreactive and enkephalin-immunoreactive profiles in the cat spinal cord were examined using combined pre-embedding immunoperoxidase and post-embedding immunogold electron microscopic immunocytochemistry. Although colchicine was not used, enkephalin...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(92)90501-w
更新日期:1992-04-13 00:00:00
abstract::The modulation of cutaneomuscular responses in response to mechanical vibration applied to the foot sole and to the ankle tendons was established in ten healthy subjects. The effects of mechanical vibration applied to the skin adjacent to the tibialis anterior (TA) and Achilles tendons were examined in two subjects. W...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2013.04.042
更新日期:2013-06-17 00:00:00
abstract::The dopaminergic system has a long history of being associated with reward-related activities but the developmental consequences of blocking dopamine receptor function on reward-based associative learning has been less studied. To this end, male, Long Evans rats were systemically (i.p.) treated with the dopamine recep...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2020.134917
更新日期:2020-04-23 00:00:00
abstract::Spinocerebellar ataxias (SCAs) are a group of clinically and genetically heterogeneous neurological diseases. The expansion of unstable microsatellite repeats has been identified as the underlying pathogenic cause of 10 subtypes of autosomal dominant SCAs. The aetiology of sporadic SCA is unknown. The aim of this stud...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2007.09.055
更新日期:2007-12-11 00:00:00
abstract::The mammalian Period1 gene is rhythmically expressed and its proteins are found within the nucleus of the cells of the suprachiasmatic nuclei (SCN), the central circadian pacemaker in mammals; however, whether the target of the PER1 proteins is also the nucleus in the retinal peripheral clock cells is yet to be determ...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2007.03.054
更新日期:2007-05-23 00:00:00
abstract::This study evaluates the potential neuroprotective properties of amburoside A, a glucoside isolated from Amburana cearensis, on rat mesencephalic cell cultures exposure to the neurotoxin 6-hydroxydopamine (6-OHDA). The parameters determined were cell viability by the 3[4,5-dimethylthiazole-2-il]-2,5-diphenyltetrazoliu...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2005.06.034
更新日期:2005-11-11 00:00:00
abstract::Electrical stimulation of the spinal tract of the trigeminal nerve at 5 Hz to elicit the trigeminal depressor response in anesthetized rabbits also causes an acute fall in ear pinna blood flow to near zero levels (from 31+/-8 to 2+/-2 kHz, n = 5, P < 0.01). This active vasoconstriction in the ear artery contrasts with...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(98)00717-4
更新日期:1998-10-23 00:00:00
abstract::Central dopaminergic systems have been implicated in the regulation of blood pressure. We examined the effect on blood pressure of electrical or chemical stimulation of the rat brain ventral tegmental area (VTA) which is the region of origin of the A10 dopaminergic system. Electrical stimulation in urethane-anaestheti...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(94)90068-x
更新日期:1994-08-01 00:00:00
abstract::The extracellular concentrations of the lipid peroxidation product malonaldehyde (MDA) and the antioxidants ascorbic and uric acid were measured in rat brain in vivo using microdialysis coupled to HPLC with ultra violet spectrophotometry. Treatment with kainic acid at pH 4.1 (50 nmol) caused a significant increase in ...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(95)12073-d
更新日期:1995-11-10 00:00:00
abstract::To study zinc (Zn) mobility in the rat olfactory tract, brain distribution of 65Zn after injection into the olfactory bulb or amygdaloid nuclei was analyzed by autoradiography. Twenty-four hours after 65Zn injection into the olfactory bulb, 65Zn was distributed in the ipsilateral piriform cortex, amygdaloid nuclei and...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(97)00181-x
更新日期:1997-03-28 00:00:00