Abstract:
:The findings of mutations and the development of targeted therapies have improved lung cancer management. Still, the prognosis remains poor, and we need to know more about the genetic and epigenetic alterations in lung cancer. MicroRNAs are involved in crucial biological processes like carcinogenesis by regulating gene expression at the post-transcriptional level. In this project, we have studied the microRNA expression of lung adenocarcinomas and corresponding normal lung tissue and correlated the expression with clinical data and EGFR- and KRAS-mutational status. Agilent microarrays have been used, examining microRNA expression in 154 surgically resected lung adenocarcinomas and 20 corresponding normal lung tissue samples. Findings were confirmed by RT-qPCR in the same cohort and in an independent cohort of 103 lung cancer patients. EGFR and KRAS mutation analyses were also performed. 129 microRNAs were significantly differentially expressed in lung adenocarcinomas compared with normal lung tissue, and 17 microRNAs were differentially expressed between EGFR-mutated and EGFR wildtype tumors. We identified microRNAs associated with time to progression. We have identified several aberrantly expressed microRNAs that discriminate lung adenocarcinomas from normal lung tissue, and hence may be potential biomarkers for early detection. We have found microRNAs that are differentially expressed between EGFR-mutated and EGFR wildtype lung adenocarcinomas, suggesting that microRNAs can be used as molecular biomarkers in classification. We hypothesize that microRNA expression can be used as biomarkers for clinical course.
journal_name
Int J Cancerjournal_title
International journal of cancerauthors
Bjaanaes MM,Halvorsen AR,Solberg S,Jørgensen L,Dragani TA,Galvan A,Colombo F,Anderlini M,Pastorino U,Kure E,Børresen-Dale AL,Brustugun OT,Helland Adoi
10.1002/ijc.28828subject
Has Abstractpub_date
2014-10-15 00:00:00pages
1812-21issue
8eissn
0020-7136issn
1097-0215journal_volume
135pub_type
杂志文章abstract::Retinoids modulate several cell functions and especially inhibit the growth of a wide variety of cells including breast cancer. Retinoic acid receptor-gamma (RAR-gamma) has been shown to mediate the antiproliferative activity of retinoids. To further test this hypothesis we examined the effects of different RAR-gamma ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19970502)71:3<497::aid-ijc
更新日期:1997-05-02 00:00:00
abstract::Results of epidemiologic studies of physical activity and ovarian cancer risk are inconsistent. Few have attempted to measure physical activity over the lifetime or in specific age windows, which may better capture etiologically relevant exposures. We examined participation in moderate-to-vigorous recreational physica...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.32513
更新日期:2020-04-01 00:00:00
abstract::The alpha 5 beta 1 integrin (CD49e/CD29), a heterodimeric membrane protein, is the "classical" fibronectin receptor on many cell types. During B-cell ontogeny, expression of the alpha 5-subunit is developmentally regulated. The alpha 5 beta 1 is decisive for migration on fibronectin substrate and very likely cooperate...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910600623
更新日期:1995-03-16 00:00:00
abstract::Interest has increased in the potential role of circulating tumour cells in cancer management. Most cell-based studies have been designed to determine the number of circulating tumour cells in a given volume of blood. Ability to understand the biology of the cancer cells would increase the clinical potential. The purp...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.29680
更新日期:2016-01-01 00:00:00
abstract::Laser scanning confocal microscopy has been used to follow the uptake and efflux of the 2-fluoroglycoside of doxorubicin, ME2303, in live cultures of the human ovarian cancer cell line A2780 and its doxorubicin-resistant variant A2780AD. Our methods combine confocal laser scanning microscopy and image analysis to exam...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910590118
更新日期:1994-10-01 00:00:00
abstract::Diet is the primary source of cadmium-a proven Group 1 human carcinogen-for non-smokers. Observational studies investigating the effect of cadmium from food sources on breast cancer risk have produced inconsistent results. We examined the association between dietary cadmium and risk of breast cancer defined by estroge...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.32039
更新日期:2019-05-01 00:00:00
abstract::Monoclonal antibodies (MAbs) reactive with the ganglioside fucosyl GM1 (Fuc-GM1), an antigen associated with small-cell carcinoma of the lung (SCLC), were tested for their ability to mediate tumor-cell killing in vitro in conjunction with humoral and cellular effectors and to inhibit tumor engraftment in nude mice in ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910490619
更新日期:1991-12-02 00:00:00
abstract::A panel of monoclonal antibodies (MAbs) has been obtained, directed against determinants of normal human colorectal epithelium. BALB/c mice were immunized and boosted with mucosal scrapings and cell membrane preparations from normal large intestine. In one case boosting was also performed with HT29 colon carcinoma cel...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910390309
更新日期:1987-03-15 00:00:00
abstract::To study the drug delivery to tumor by utilization of an oligopeptide transport activity, we examined the accumulation of dipeptides and the peptide-mimetic anti-cancer drug, bestatin, a substrate of oligopeptide transporter PepT1. Firstly, we established HeLa cells stably expressing human peptide transporter (hPepT1)...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:
更新日期:2000-10-15 00:00:00
abstract::Increasing evidence indicates the importance of the tumor microenvironment, in particular cancer-associated fibroblasts, in cancer development and progression. In our study, we developed a novel, visually based method to identify new immunohistochemical signatures of these fibroblasts. The method employed a protein li...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.28035
更新日期:2013-07-15 00:00:00
abstract::Prostate cancer (PC) has the highest degree of genetic transmission of any form of malignancy. In some families, the hereditary pattern is so strong as to mimic an autosomal dominance trait. We reviewed the known predisposing genetic markers to assess possible strategies for screening of families at risk. We carried o...
journal_title:International journal of cancer
pub_type: 杂志文章,评审
doi:10.1002/ijc.29949
更新日期:2016-06-01 00:00:00
abstract::Using a method optimized in hepatocellular carcinoma (HCC), we established patient-derived xenograft (PDX) models with an increased take rate (42.2%) and demonstrated that FBS +10% dimethyl sulfoxide exhibited the highest tumor take rate efficacy. Among 254 HCC patients, 103 stably transplantable xenograft lines that ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.32564
更新日期:2020-03-15 00:00:00
abstract::We assessed prospectively whether residual cervical intraepithelial neoplasia (CIN) after treatment for high-grade CIN can be predicted by genotype-specific high-risk HPV (HR-HPV) detection in follow-up cervical scrapes. A broad spectrum, highly sensitive SPF(10)-LiPA-PCR HPV detection technique was used on cervical s...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.10691
更新日期:2002-11-10 00:00:00
abstract::In order to isolate and characterize genes whose expression may be altered in breast malignancy, we screened a cDNA library with a polyclonal anti-serum against breast-cancer-metastasis membranes and isolated several immunopositive clones. One of these, AJ1, was analyzed in detail and found to be expressed at varying ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910500315
更新日期:1992-02-01 00:00:00
abstract::Chemotherapy with taxanes such as paclitaxel (PTX) is a key component of triple negative breast cancer (TNBC) treatment. PTX is used in combination with other drugs in both the adjuvant setting and in advanced breast cancer. Because a proportion of patients respond poorly to PTX or relapse after its use, a greater und...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.30137
更新日期:2016-09-01 00:00:00
abstract::Paraffin sections (n = 168, 27 benign, 16 low malignant potential [LMP] and 125 malignant tumours) from epithelial ovarian tumours were evaluated immunohistochemically for expression of retinoblastoma gene product (pRB) and p53 protein, and the relationship among pRB, p53 and cyclin-dependent kinase inhibitor 2 (CDKN2...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19970822)74:4<407::aid-ijc
更新日期:1997-08-22 00:00:00
abstract::As a part of an assessment of the potential use of retinoids in preventive and adjuvant treatment of HNSCC, we examined the effects of beta-all-trans retinoic acid (RA) on the growth and cell-surface glycoconjugates of 2 HNSCC cell lines. These lines, designated 1483 and 183A, were established from an untreated patien...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910400217
更新日期:1987-08-15 00:00:00
abstract::The 9p21-23 chromosome region harbors a number of known and putative tumor-suppressor genes (TSGs). The best characterized gene in this area is p16(INK4A) (CDKN2A). Alterations of its product have been observed in various malignancies, including non-small-cell lung carcinomas (NSCLCs). We earlier investigated the mech...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(20000320)89:2<133::aid-ijc
更新日期:2000-03-20 00:00:00
abstract::Papillary thyroid carcinoma (PTC) is the most common type of thyroid cancer. Tumor recurrence occurs in ∼20% of PTCs and some reach advanced stages. Promoter mutation in the telomerase reverse transcriptase (TERT) gene is identified to be a prognostic marker in PTC. However, the contribution of TERT promoter mutation ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.31225
更新日期:2018-05-15 00:00:00
abstract::In order to study the mechanisms responsible for resistance to CDDP, 5 human tumor cell lines were made resistant to CDDP by repeated in vitro exposures. After cloning it was found that the cell lines developed were between 3.3-fold and 17-fold more resistant to CDDP than the parental cell lines at the IC90. These lin...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910470214
更新日期:1991-01-21 00:00:00
abstract::The Cancer Incidence in Five Continents (CI5) series comprises nine volumes that bring together peer-reviewed results from population-based cancer registries worldwide. The aim of each is to make available comparable data on cancer incidence from as wide a range of geographical locations as possible. In addition, the ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.25517
更新日期:2010-12-15 00:00:00
abstract::It has been debated whether the reported increased risk of Hodgkin's lymphoma (HL) after tonsillectomy could be due to some underlying factor rather than the surgery itself. We studied whether not only tonsillectomy but also tonsillitis was associated with HL. This nationwide cohort study included all Danish residents...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.24973
更新日期:2010-08-01 00:00:00
abstract::Our previous studies demonstrate that introduction of a approximately 70 cM region (now estimated at 63.75 Mb by the Human Genome Project) of human chromosome 12 into the highly metastatic Dunning rat prostate cancer cell line AT6.1 results in >30-fold (>/=90%) reduction in the number of overt metastases in spontaneou...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.11483
更新日期:2004-01-01 00:00:00
abstract::A marked increase in natural killer (NK) activity is observed with lymphoid cells prepared from SJL/J mouse spleen and lymph nodes, in which a transplantable reticulum-cell neoplasm (RCN) is growing. The killer cells are non-adherent, non-phagocytic, relatively resistant to X-ray, and scarcely or only partially inacti...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910250316
更新日期:1980-03-15 00:00:00
abstract::The incidence of cutaneous melanoma in children aged 0-14 years was examined in Queensland, Australia. Details of notifications were collected from the population-based Australian Paediatric Cancer Registry. Between 1987 and 1994, the age-adjusted incidence rates of invasive cutaneous melanoma occurring in Queensland ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910630602
更新日期:1995-12-11 00:00:00
abstract::Eight squirrel monkeys (Saimiri sciureus) challenged with EBV or EBV-transformed SLCL were naturally or experimentally infected with Plasmodium knowlesi or Pl. brasilianum. Most of the animals had been splenectomized and unilaterally nephrectomized. Three of these monkeys received one dose of 6 to 12 X 10(8) autologou...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910170417
更新日期:1976-04-15 00:00:00
abstract::In advanced stages of malignant melanoma (MM), metastases to the CNS are frequently observed. Few results are available on trophic factors and immunological features involved in the process of invasion and adhesion of circulating metastatic cells into the CNS. A direct comparison of remote metastases found in differen...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19990924)83:1<135::aid-ijc
更新日期:1999-09-24 00:00:00
abstract::The nature of 15 immortal mouse liver epithelial cell (MLEC) lines established from normal C3H mice was investigated specifically in terms of ras oncogene activation. Neither transforming activity nor point mutation within codon 61 of c-H-ras could be demonstrated in any of the cell lines by DNA transfection in a NIH/...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910470112
更新日期:1991-01-02 00:00:00
abstract::Although the incidence of testis cancer has risen markedly in many Western populations over the past half-century, it is not clear whether rates in other populations also have increased. To clarify this issue, we examined testis cancer incidence rates over the 25-year time period of 1973-1997 for selected populations ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.20931
更新日期:2005-07-10 00:00:00
abstract::Medulloblastoma is a primitive neuroectodermal tumor of the cerebellum with poorly understood pathogenesis. Previous studies have reported loss of heterozygosity (LOH) on chromosome arms 17p, 11p and 9q and cytogenetic abnormalities of chromosome 1 in medulloblastoma. We have used the polymerase chain reaction to ampl...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0215(19960703)67:1<11::AID-IJC3
更新日期:1996-07-03 00:00:00