当前位置： SCI文献检索 > GASTROENTEROLOGY期刊下所有文献 > Emergence of antigenic glycoprotein structures in ulcerative colitis detected through monoclonal antibodies.

Emergence of antigenic glycoprotein structures in ulcerative colitis detected through monoclonal antibodies.

Abstract：

:The emergence of new glycoprotein structures in colonic mucosa from patients with ulcerative colitis (UC) was assessed through the development of monoclonal antibodies (MAbs). Hybridomas were prepared from mice immunized with mucin glycoproteins purified from UC colonic tissue. Supernatants of 11 fusion products among 1200 fusion products that were screened in a solid-phase binding assay differentially bound UC-derived colonic mucin glycoproteins relative to comparable preparations from normal or Crohn's colitis tissue. These hybridomas were double-cloned to yield MAbs designated as MAbs UC 1-11. Disease-related specificity of MAbs UC 1-11 was determined through assessment of binding to beads coated with mucin glycoproteins purified from individual samples of UC tissue (n = 15), normal tissue (n = 21), and Crohn's colitis tissue (n = 10). Monoclonal antibody UC 7, an MAb of immunoglobulin G2A subclass, showed differential binding in solid-phase assays to UC mucin glycoproteins, with a mean binding of 10,170 +/- 2740 cm per UC glycoprotein-coated bead versus 2300 +/- 1080 and 2470 +/- 1525 cpm for normal and Crohn's colitis-derived glycoproteins, respectively. Monoclonal antibody UC 11 showed similar differential binding to UC mucin glycoproteins (9860 +/- 680 cpm vs. 1770 +/- 420 cpm). Binding specificity in solid-phase assay was mirrored by colonic mucosal staining patterns assessed by indirect immunofluorescent staining. Monoclonal antibody UC 7 specifically stained colonic mucosa from 8 of 10 patients with active UC, none of the samples from 8 normal controls, and none of the samples from 11 disease controls (six with Crohn's colitis, five with other inflammatory disorders). Specific staining was present on both the epithelial surface and on cells scattered within the lamina propria. Staining by MAb UC 7 was also observed in 3 of 4 samples of proximal uninvolved mucosa from patients with left-sided ulcerative colitis and in 3 of 5 samples from UC patients without acute disease activity.

journal_name

Gastroenterology

journal_title

Gastroenterology

authors

Podolsky DK,Fournier DA

doi

10.1016/0016-5085(88)90493-3

subject

Has Abstract

pub_date

1988-08-01 00:00:00

pages

371-8

issue

2

eissn

0016-5085

issn

1528-0012

pii

0016-5085(88)90493-3

journal_volume

95

pub_type

杂志文章

相关文献

GASTROENTEROLOGY文献大全
  • Intestinal infection with Giardia spp. reduces epithelial barrier function in a myosin light chain kinase-dependent fashion.

    abstract：BACKGROUND & AIMS:Giardiasis causes malabsorptive diarrhea, and symptoms can be present in the absence of any significant morphologic injury to the intestinal mucosa. The effects of giardiasis on epithelial permeability in vivo remain unknown, and the role of T cells and myosin light chain kinase (MLCK) in altered inte...

    journal_title：Gastroenterology

    pub_type： 杂志文章

    doi：10.1053/gast.2002.36002

    authors： Scott KG,Meddings JB,Kirk DR,Lees-Miller SP,Buret AG

    更新日期：2002-10-01 00:00:00

  • Integrin alpha6beta4 as a suppressor and a predictive marker for peritoneal dissemination in human gastric cancer.

    abstract：BACKGROUND & AIMS:Because alterations of integrin expression in cancers contribute to cancer cell biology, we analyzed the association between the potential for peritoneal dissemination and integrin expression. METHODS:The dissemination potential of 10 human gastric cancer cell lines in mice with severe combined immun...

    journal_title：Gastroenterology

    pub_type： 杂志文章

    doi：10.1016/s0016-5085(00)70255-1

    authors： Ishii Y,Ochiai A,Yamada T,Akimoto S,Yanagihara K,Kitajima M,Hirohashi S

    更新日期：2000-03-01 00:00:00

  • Pancreatic stenting prevents pancreatitis after biliary sphincterotomy in patients with sphincter of Oddi dysfunction.

    abstract：BACKGROUND & AIMS:Patients with sphincter of Oddi dysfunction are at high risk of developing pancreatitis after endoscopic biliary sphincterotomy. Impaired pancreatic drainage caused by pancreatic sphincter hypertension is the likely explanation for this increased risk. A prospective, randomized controlled trial was co...

    journal_title：Gastroenterology

    pub_type： 临床试验,杂志文章,随机对照试验

    doi：10.1016/s0016-5085(98)70031-9

    authors： Tarnasky PR,Palesch YY,Cunningham JT,Mauldin PD,Cotton PB,Hawes RH

    更新日期：1998-12-01 00:00:00

  • The SH3 binding motif of HCV [corrected] NS5A protein interacts with Bin1 and is important for apoptosis and infectivity.

    abstract：BACKGROUND & AIMS:HCV nonstructural protein 5A (NS5A) has been implicated in regulating cell growth and interferon response. The NS5A protein contains proline-rich regions that are highly conserved among HCV genotypes and match Src homology 3 (SH3)-binding motifs (PxxP) found in various cellular signaling molecules. M...

    journal_title：Gastroenterology

    pub_type： 杂志文章

    doi：10.1053/j.gastro.2005.12.030

    authors： Nanda SK,Herion D,Liang TJ

    更新日期：2006-03-01 00:00:00

  • The topographic representation of esophageal motor function on the human cerebral cortex.

    abstract：BACKGROUND & AIMS:Corticofugal pathways to the human esophagus have been recently identified in humans using transcranial magnetic stimulation. The aim of this study was to determine the topographic representation of the esophagus on the cerebral cortex. METHODS:Focal magnetic stimulation of 1-cm areas was performed o...

    journal_title：Gastroenterology

    pub_type： 杂志文章

    doi：10.1016/s0016-5085(96)70053-7

    authors： Aziz Q,Rothwell JC,Hamdy S,Barlow J,Thompson DG

    更新日期：1996-10-01 00:00:00

  • Sulfate-sodium cotransport by brush-border membrane vesicles isolated from rat ileum.

    abstract：:Uptake of inorganic sulfate into brush-border membrane vesicles isolated by a calcium precipitation method from rat small intestine was investigated using a rapid filtration technique and 35Sulfur acid as tracer. Sulfate uptake by membrane vesicles was osmotically sensitive, suggesting transport into an intravesicular...

    journal_title：Gastroenterology

    pub_type： 杂志文章

    doi：

    authors： Lücke H,Stange G,Murer H

    更新日期：1981-01-01 00:00:00

  • Epigenetic Homogeneity Within Colorectal Tumors Predicts Shorter Relapse-Free and Overall Survival Times for Patients With Locoregional Cancer.

    abstract：BACKGROUND & AIMS:There are few validated biomarkers that can be used to predict outcomes for patients with colorectal cancer. Part of the challenge is the genetic and molecular heterogeneity of colorectal tumors not only among patients, but also within tumors. We have explored intratumor heterogeneity at the epigeneti...

    journal_title：Gastroenterology

    pub_type： 杂志文章

    doi：10.1053/j.gastro.2016.08.001

    authors： Martínez-Cardús A,Moran S,Musulen E,Moutinho C,Manzano JL,Martinez-Balibrea E,Tierno M,Élez E,Landolfi S,Lorden P,Arribas C,Müller F,Bock C,Tabernero J,Esteller M

    更新日期：2016-11-01 00:00:00

  • IW-3718 Reduces Heartburn Severity in Patients With Refractory Gastroesophageal Reflux Disease in a Randomized Trial.

    abstract：BACKGROUND & AIMS:Refractory gastroesophageal reflux disease (GERD) reduces quality of life and creates significant financial burden on the health care system. Approximately 30% of patients with GERD who receive label-dose proton pump inhibitors (PPIs) still have symptoms. We performed a trial to evaluate the efficacy ...

    journal_title：Gastroenterology

    pub_type： 杂志文章

    doi：10.1053/j.gastro.2020.02.031

    authors： Vaezi MF,Fass R,Vakil N,Reasner DS,Mittleman RS,Hall M,Shao JZ,Chen Y,Lane L,Gates AM,Currie MG

    更新日期：2020-06-01 00:00:00

  • Erythropoietic protoporphyria: unusual skin and neurological problems after liver transplantation.

    abstract：:The case of a woman with protoporphyria who developed liver failure and underwent liver transplantation is described. During the pretransplant episode of liver failure she developed quadriparesis that rapidly progressed after transplantation to a severe polyneuropathy. Following transplantation she also developed a se...

    journal_title：Gastroenterology

    pub_type： 杂志文章

    doi：10.1016/0016-5085(91)90680-j

    authors： Herbert A,Corbin D,Williams A,Thompson D,Buckels J,Elias E

    更新日期：1991-06-01 00:00:00

  • Gastric mucosal alpha(4)beta(7)-integrin-positive CD4 T lymphocytes and immune protection against helicobacter infection in mice.

    abstract：BACKGROUND & AIMS:The integrin alpha(4)beta(7) mediates homing of effector/memory lymphocytes to the intestine and the mucosa-associated lymphoid tissue. This study examined the ability of alpha(4)beta(7)(hi) CD4(+) T lymphocytes to home to the stomach and their role in immunization-mediated protection against Helicoba...

    journal_title：Gastroenterology

    pub_type： 杂志文章

    doi：10.1053/gast.2000.8548

    authors： Michetti M,Kelly CP,Kraehenbuhl JP,Bouzourene H,Michetti P

    更新日期：2000-07-01 00:00:00

  • Bicarbonate and cyclic AMP content of pure human pancreatic juice in response to graded doses of synthetic secretin.

    abstract：:In seven healthy volunteers pure pancreatic juice was obtained by endoscopic cannulation of the papilla of Vater. Synthetic secretin was intravenously infused in doses doubled every 20 min. The volume of pancreatic juice was proportional to the log of the secretin dose. A significant rise (P less than 0.05) in pancrea...

    journal_title：Gastroenterology

    pub_type： 杂志文章

    doi：

    authors： Domschke S,Domschke W,Rösch W,Konturek SJ,Wünsch E,Demling L

    更新日期：1976-04-01 00:00:00

  • Comparison of endogenous and radiolabeled bile acid excretion in patients with idiopathic chronic diarrhea.

    abstract：:Fecal recovery of radioactivity after ingestion of a bolus of radiolabeled bile acid is abnormally high in most patients with idiopathic chronic diarrhea. To evaluate the significance of this malabsorption, concurrent fecal excretion of both exogenous radiolabeled bile acid and endogenous (unlabeled) bile acid were me...

    journal_title：Gastroenterology

    pub_type： 杂志文章

    doi：10.1016/0016-5085(90)90031-u

    authors： Schiller LR,Bilhartz LE,Santa Ana CA,Fordtran JS

    更新日期：1990-04-01 00:00:00

  • Pathogenic and protective roles of MyD88 in leukocytes and epithelial cells in mouse models of inflammatory bowel disease.

    abstract：BACKGROUND & AIMS:Toll-like receptors (TLR) are innate immune receptors involved in recognition of the intestinal microflora; they are expressed by numerous cell types in the intestine, including epithelial cells, myeloid cells, and lymphocytes. Little is known about the relative contributions of TLR signaling in disti...

    journal_title：Gastroenterology

    pub_type： 杂志文章

    doi：10.1053/j.gastro.2010.04.045

    authors： Asquith MJ,Boulard O,Powrie F,Maloy KJ

    更新日期：2010-08-01 00:00:00

  • Pancreatic, gallbladder, and gastric responses to intraduodenal calcium perfusion in man.

    abstract：:To determine the effect of intraduodenal calcium on pancreatic, gallbladder, and gastric functions in healthy man, a validated perfusion method was employed to quantify total pancreatic, biliary, and gastric outputs during duodenal perfusion of either 6 mM, 12 mM,or 25 mM of elemental calcium (as isotonic calcium chlo...

    journal_title：Gastroenterology

    pub_type： 杂志文章

    doi：

    authors： Holtermuller KH,Mallagelada JR,McCall JT,Go VL

    更新日期：1976-05-01 00:00:00

  • Serum bile acid concentrations during pregnancy and their relationship to obstetric cholestasis.

    abstract：:We have prospectively studied changes in serum postprandial cholylglycine (CG) concentration during 297 pregnancies. We found an increase in CG concentration from 0.3 mumol/L at 15 weeks' pregnancy to 0.6 mumol/L at 40 weeks' pregnancy. Although this increase was statistically significant (p less than 0.005), median c...

    journal_title：Gastroenterology

    pub_type： 杂志文章

    doi：10.1016/0016-5085(86)90682-7

    authors： Lunzer M,Barnes P,Byth K,O'Halloran M

    更新日期：1986-10-01 00:00:00

  • Vitamin E reduces oxidant injury to mitochondria and the hepatotoxicity of taurochenodeoxycholic acid in the rat.

    abstract：BACKGROUND & AIMS:Hydrophobic bile acids have been implicated in the pathogenesis of cholestatic liver injury. The hypothesis that hydrophobic bile acid toxicity is mediated by oxidant stress in an in vivo rat model was tested in this study. METHODS:A dose-response study of bolus intravenous (i.v.) taurochenodeoxychol...

    journal_title：Gastroenterology

    pub_type： 杂志文章

    doi：10.1016/s0016-5085(98)70644-4

    authors： Sokol RJ,McKim JM Jr,Goff MC,Ruyle SZ,Devereaux MW,Han D,Packer L,Everson G

    更新日期：1998-01-01 00:00:00

  • A magnetic resonance study of the effect of nutritional status on cold-preserved murine liver.

    abstract：BACKGROUND/AIMS:Clinical and experimental studies suggest a link between nutritional status and the recovery of hepatic function after hypoxic and hypothermic insults. This study aimed at determining the metabolic pathways involved in such recovery as a function of nutrition. METHODS:Livers from fed and fasted mice we...

    journal_title：Gastroenterology

    pub_type： 杂志文章

    doi：10.1016/0016-5085(94)90819-2

    authors： Dimicoli JL,Patry J,Adam R,Dennison A,Nedelec JF,Bismuth H

    更新日期：1994-12-01 00:00:00

  • APC and oncogenic KRAS are synergistic in enhancing Wnt signaling in intestinal tumor formation and progression.

    abstract：BACKGROUND & AIMS:Synchronous activation of the Wnt signaling pathway, mostly because of loss of function of the APC tumor suppressor, and of the oncogenic KRAS-signaling pathway is very frequent in colorectal cancer and is associated with poor prognosis. METHODS:We have generated a compound transgenic mouse model, KR...

    journal_title：Gastroenterology

    pub_type： 杂志文章

    doi：10.1053/j.gastro.2006.08.011

    authors： Janssen KP,Alberici P,Fsihi H,Gaspar C,Breukel C,Franken P,Rosty C,Abal M,El Marjou F,Smits R,Louvard D,Fodde R,Robine S

    更新日期：2006-10-01 00:00:00

  • Cytomegalovirus infection of the appendix in patient with the acquired immunodeficiency syndrome.

    abstract：:Disseminated cytomegalovirus infection occurs predominantly in immunocompromised hosts. Involvement of the gastrointestinal tract in the acquired immunodeficiency syndrome is frequent, but to our knowledge there is only one report of involvement of the appendix. In this study, a patient with a history of the acquired ...

    journal_title：Gastroenterology

    pub_type： 杂志文章

    doi：10.1016/0016-5085(91)90485-4

    authors： Valerdiz-Casasola S,Pardo-Mindan FJ

    更新日期：1991-07-01 00:00:00

  • Influence of endogenous prostaglandins on secretin-mediated inhibition of gastric acid secretion in dogs.

    abstract：:The influence of endogenous prostaglandins on secretin-mediated inhibition of gastric acid secretion was examined in 6 mongrel dogs with Thomas gastric and Herrera pancreatic cannulas. The dogs were given intravenous pentagastrin (1 microgram/kg.h) during the 180-min experiment, and graded doses of secretin (0.3-1.5 m...

    journal_title：Gastroenterology

    pub_type： 杂志文章

    doi：10.1016/s0016-5085(88)80007-6

    authors： MacLellan DG,Upp JR Jr,Thompson JC

    更新日期：1988-09-01 00:00:00

  • A 1-year trial of telbivudine, lamivudine, and the combination in patients with hepatitis B e antigen-positive chronic hepatitis B.

    abstract：BACKGROUND & AIMS:A previous 4-week trial of telbivudine in patients with chronic hepatitis B indicated marked antiviral effects with good tolerability, leading to the present 1-year phase 2b trial. METHODS:This randomized, double-blind, multicenter trial evaluated the efficacy and safety of telbivudine 400 or 600 mg/...

    journal_title：Gastroenterology

    pub_type： 临床试验,杂志文章,多中心研究,随机对照试验

    doi：10.1016/j.gastro.2005.05.053

    authors： Lai CL,Leung N,Teo EK,Tong M,Wong F,Hann HW,Han S,Poynard T,Myers M,Chao G,Lloyd D,Brown NA,Telbivudine Phase II Investigator Group.

    更新日期：2005-08-01 00:00:00

  • Liver adenomatosis. An entity distinct from liver adenoma?

    abstract：:From 1979 to 1984, we followed the cases of 3 men (aged 13, 31, and 75 yr) and 2 women (aged 38 and 45 yr who had never used oral contraceptives) suffering from liver adenomatosis, an uncommon lesion consisting of numerous benign adenomas in an otherwise normal hepatic parenchyma. During the same period, we observed 2...

    journal_title：Gastroenterology

    pub_type： 杂志文章

    doi：

    authors： Flejou JF,Barge J,Menu Y,Degott C,Bismuth H,Potet F,Benhamou JP

    更新日期：1985-11-01 00:00:00

  • Effects of short-term pancreatic duct obstruction in rats.

    abstract：:The short-term effects of rat pancreatic duct obstruction were evaluated and compared with those recently reported to follow obstruction of the rabbit pancreatic duct. In both species pancreatic edema and hyperamylasemia are noted, and the lysosomal hydrolase cathepsin B is redistributed from the lysosome-enriched to ...

    journal_title：Gastroenterology

    pub_type： 杂志文章

    doi：10.1016/0016-5085(91)90601-g

    authors： Ohshio G,Saluja A,Steer ML

    更新日期：1991-01-01 00:00:00

  • Bile-salt metabolism in the primate fetus.

    abstract：:Fetal bile salt metabolism was assessed by the intravenous infusion of e114C]cholate into 6 monkey fetuses. The placental transfer, fetal plasma clearance, and fetal hepatic excretion of the administered radiolabeled bile salt were measured. Placental transfer averaged 30% of the injected dose, while biliary excretion...

    journal_title：Gastroenterology

    pub_type： 杂志文章

    doi：

    authors： Little JM,Smallwood RA,Lester R,Piasecki GJ,Jackson BT

    更新日期：1975-12-01 00:00:00

  • Verification of the lactase site of rat lactase-phlorizin hydrolase by site-directed mutagenesis.

    abstract：BACKGROUND & AIMS:Lactase-phlorizin hydrolase (LPH) is an intestinal microvillus membrane glycoprotein that hydrolyzes lactose and phlorizin. These enzymatic activities have been assigned to glutamic acid (E) residues 1271 and 1747 in rabbit LPH. The aim of this study was to determine directly if this assignment was co...

    journal_title：Gastroenterology

    pub_type： 杂志文章

    doi：10.1016/0016-5085(95)90583-9

    authors： Neele AM,Einerhand AW,Dekker J,Büller HA,Freund JN,Verhave M,Grand RJ,Montgomery RK

    更新日期：1995-10-01 00:00:00

  • Germline mutations of EXO1 gene in patients with hereditary nonpolyposis colorectal cancer (HNPCC) and atypical HNPCC forms.

    abstract：BACKGROUND & AIMS:Germline mutations in one of four mismatch repair genes have been found in the majority of families with hereditary nonpolyposis colorectal cancer (HNPCC), but only in a small part of families with atypical HNPCC. The recently cloned EXO1 gene might be involved in the pathogenesis of HNPCC because the...

    journal_title：Gastroenterology

    pub_type： 杂志文章

    doi：10.1053/gast.2001.25117

    authors： Wu Y,Berends MJ,Post JG,Mensink RG,Verlind E,Van Der Sluis T,Kempinga C,Sijmons RH,van der Zee AG,Hollema H,Kleibeuker JH,Buys CH,Hofstra RM

    更新日期：2001-06-01 00:00:00

  • Role of acquired and hereditary thrombotic risk factors in colon ischemia of ambulatory patients.

    abstract：BACKGROUND & AIMS:Hypercoagulable states may play an important role in the pathogenesis of colon ischemia. Aim of this study was to assess this hypothesis investigating the role of acquired and hereditary thrombotic risk factors in patients with definite diagnosis of colon ischemia. METHODS:We compared the frequency o...

    journal_title：Gastroenterology

    pub_type： 杂志文章

    doi：10.1053/gast.2001.27227

    authors： Koutroubakis IE,Sfiridaki A,Theodoropoulou A,Kouroumalis EA

    更新日期：2001-09-01 00:00:00

  • Mesenchymal Plasticity Regulated by Prrx1 Drives Aggressive Pancreatic Cancer Biology.

    abstract：BACKGROUND & AIMS:Pancreatic ductal adenocarcinoma (PDAC) is characterized by a fibroblast-rich desmoplastic stroma. Cancer-associated fibroblasts (CAFs) have been shown to display a high degree of interconvertible states including quiescent, inflammatory, and myofibroblastic phenotypes; however, the mechanisms by whic...

    journal_title：Gastroenterology

    pub_type： 杂志文章

    doi：10.1053/j.gastro.2020.09.010

    authors： Feldmann K,Maurer C,Peschke K,Teller S,Schuck K,Steiger K,Engleitner T,Öllinger R,Nomura A,Wirges N,Papargyriou A,Jahan Sarker RS,Ranjan RA,Dantes Z,Weichert W,Rustgi AK,Schmid RM,Rad R,Schneider G,Saur D,Reichert

    更新日期：2021-01-01 00:00:00

  • Negative regulation of liver regeneration by innate immunity (natural killer cells/interferon-gamma).

    abstract：BACKGROUND AND AIMS:Hepatic lymphocytes are composed mainly of natural killer (NK) cells and NKT cells, which play key roles in innate immune responses against pathogens and tumors in the liver. This report analyzes the effects of activation of innate immunity by viral infection or the toll-like receptor 3 (TLR3) ligan...

    journal_title：Gastroenterology

    pub_type： 杂志文章

    doi：10.1053/j.gastro.2004.08.055

    authors： Sun R,Gao B

    更新日期：2004-11-01 00:00:00

  • Isolation and characterization of normal and neoplastic colonic epithelial cell populations.

    abstract：:The aim of the present study was to characterize rat mucosal colonic cells harvested from the crypt continuum during differentiation and dimethylhydrazine-induced neoplasia. The collection of colonocytes was performed using a modified nonenzymatic isolation procedure based on Ca2+ chelation and gentle mechanical disso...

    journal_title：Gastroenterology

    pub_type： 杂志文章

    doi：10.1016/0016-5085(91)80013-y

    authors： Schwartz B,Avivi C,Lamprecht SA

    更新日期：1991-03-01 00:00:00