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Metalloestrogen cadmium stimulates proliferation of stromal cells derived from the eutopic endometrium of women with endometriosis.

Abstract：

OBJECTIVE:To assess the effect of metalloestrogen cadmium (Cd) on the proliferation of endometrial stromal cells (ESC) isolated from the eutopic endometrium of women with endometriosis MATERIALS AND METHODS:ESC were isolated from eutopic endometrial samples from 10 women with endometriosis and 10 women without endometriosis. ESC cultures were established and maintained in RPMI medium. Cultures were treated with Cd at a concentration of 10(-6) M and after 24 hours and 48 hours, cell number was counted using the Neubauer hemocytometer to calculate the relative cell proliferation. At 48 hours, the 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) assay was used to test the effect of different concentrations (10(-8) M to 10(-3) M) of Cd on ESC cultures. Relative cell proliferation and MTT assay results were analyzed with ANOVA. RESULTS:At 48 hours, Cd-induced ESC proliferation was higher (p = 0.02) in women with endometriosis than in women without endometriosis, which was confirmed by the MTT assay. CONCLUSION:The results of this study demonstrate the ability of metalloestrogen Cd to induce the proliferation of stromal cells derived from the eutopic endometrium of women with endometriosis.

journal_name

Taiwan J Obstet Gynecol

journal_title

Taiwanese journal of obstetrics & gynecology

authors

Silva N,Tennekoon K,Senanayake H,Samarakoon S

doi

10.1016/j.tjog.2013.10.015

subject

Has Abstract

pub_date

2013-12-01 00:00:00

pages

540-5

issue

4

eissn

1028-4559

issn

1875-6263

pii

S1028-4559(13)00178-2

journal_volume

52

pub_type

杂志文章

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