Abstract:
:The effects of several protein modifying reagents, including phenoxybenzamine, N-ethylmaleimide (NEM), 5,5'-dithiobis (2-nitro)--benzoic acid (DTNB), p-chloromercuryphenyl sulfonic acid (PCMP) and p-bromophenacylbromide (PBPB), on the binding of 125I-Bolton Hunter substance P (125I-BHSP), 125I-Bolton Hunter eledoisin (125I-BHELE) and 3H-neurokinin B (3H-NKB)4 to cortical synaptosomes were examined. PCMP (10(-4) M), DTNB (10(-4) M) and NEM (10(-3) M) were without effect on the 125I-BHSP binding but reduced markedly the specific binding of 125I-BHELE or 3H-NKB. Although phenoxybenzamine and PBPB inhibited the 125I-BHSP binding when used in high concentrations (10(-4) M and 10(-3) M), they were more potent in inhibiting the 125I-BHELE or 3H-NKB binding. These results indicate that the NKB binding site is more sensitive to alkylating reagents than the SP binding sites and that these reagents can be used to distinguish SP and NKB receptors in the brain.
journal_name
Neuropeptidesjournal_title
Neuropeptidesauthors
Bergström L,Beaujouan JC,Torrens Y,Saffroy M,Glowinski Jdoi
10.1016/0143-4179(87)90053-9subject
Has Abstractpub_date
1987-02-01 00:00:00pages
151-9issue
2eissn
0143-4179issn
1532-2785pii
0143-4179(87)90053-9journal_volume
9pub_type
杂志文章相关文献
NEUROPEPTIDES文献大全abstract:OBJECTIVE:Neuropeptide Y, a widely circulating neurotransmitter, plays a pivotal role in energy balance, immunomodulation and asthma, and several NPY polymorphisms are promising genetic risk factors for asthma and obesity. We explored the associations of candidate NPY gene polymorphisms with prevalent asthma and its re...
journal_title:Neuropeptides
pub_type: 杂志文章
doi:10.1016/j.npep.2016.07.003
更新日期:2016-10-01 00:00:00
abstract::To find out, whether microheterogeneity of neurophysins is due to aging of neurosecretory granules we investigated neurophysin proteins of porcine origin from newly formed and aged granules, as well as from two different extraction methods from whole posterior pituitary glands by high performance liquid chromatography...
journal_title:Neuropeptides
pub_type: 杂志文章
doi:10.1016/0143-4179(83)90003-3
更新日期:1983-12-01 00:00:00
abstract::Immunohistochemical tests have demonstrated for the first time the presence of endothelins in the neuroendocrine cells of fish gill. We have sought co-localization of endothelins with serotonin and neuropeptides which are regarded as neuroendocrine markers of pulmonary diffuse neuroendocrine systems in higher vertebra...
journal_title:Neuropeptides
pub_type: 杂志文章
doi:10.1016/s0143-4179(96)90055-4
更新日期:1996-02-01 00:00:00
abstract::Humanin (HN) is an endogenous 24-residue peptide. A highly potent HN derivative, S14G-HN, which has a substitution of serine 14 to glycine, reduced amyloid burden and suppressed cognitive impairment in a mouse model of Alzheimer's disease. S14G-HN also suppressed amnesia induced by a muscarinic receptor antagonist in ...
journal_title:Neuropeptides
pub_type: 杂志文章
doi:10.1016/j.npep.2016.10.008
更新日期:2017-04-01 00:00:00
abstract::CSF methionine and leucine enkephalins were measured by high performance liquid chromatography and radioimmunoassay in diabetic patients with painful neuropathy (n = 22) and painless neuropathy (n = 5), and non-diabetic subjects with low back pain (n = 11). Wide variations in CSF enkephalin levels were found and they ...
journal_title:Neuropeptides
pub_type: 杂志文章
doi:10.1016/0143-4179(92)90067-7
更新日期:1992-06-01 00:00:00
abstract::Nerve growth factor (NGF) was reported to be increased in the serum and skin of atopic dermatitis (AD) patients, to the extent that serum nerve growth factor levels were proposed to serve as a marker of disease severity. We studied NGF levels in the serum and dermis using skin microdialysis and attempted to correlate ...
journal_title:Neuropeptides
pub_type: 杂志文章
doi:10.1016/j.npep.2011.07.008
更新日期:2011-12-01 00:00:00
abstract::The effects of graded amounts of centrally injected [intracerebroventricular, i.c.v.] oxytocin [OXT] have been studied on the steady-state level and pargyline-induced accumulation of serotonin [5-HT] in the hypothalamus, mesencephalon and dorsal hippocampus of rats. OXT only weakly affected the steady-state level of 5...
journal_title:Neuropeptides
pub_type: 杂志文章
doi:10.1016/0143-4179(84)90125-2
更新日期:1984-03-01 00:00:00
abstract::Prader-Willi syndrome (PWS) is the predominant genetic cause of obesity in humans and is associated with several behavioural phenotypes such as altered motoric function, reduced activity, and learning disabilities. It can include mood instability and, in some cases, psychotic episodes. Recently, the Snord116 gene has ...
journal_title:Neuropeptides
pub_type: 杂志文章
doi:10.1016/j.npep.2015.06.009
更新日期:2015-10-01 00:00:00
abstract::In order to study the morphological localization of the endogenous benzodiazepine ligand octadecaneuropeptide (ODN) in rat brain, we have developed antibodies against this peptide. Using a radioimmunoassay for ODN, we have observed that synthetic ODN and serial dilutions of several brain areas gave parallel displaceme...
journal_title:Neuropeptides
pub_type: 杂志文章
doi:10.1016/0143-4179(90)90155-r
更新日期:1990-01-01 00:00:00
abstract::The relative roles of hypothalamic corticotropin-releasing-hormone (CRH) and vasopressin (AVP) as mediators of the stimulant effect of vasoactive intestinal peptide (VIP) and peptide histidine isoleucine (PHI) on ACTH and corticosterone (CORT) secretion, were examined using receptor blockade of endogenous CRH and AVP....
journal_title:Neuropeptides
pub_type: 杂志文章
doi:10.1016/0143-4179(95)90112-4
更新日期:1995-03-01 00:00:00
abstract::Our previous studies showed the modulation of cocaine and amphetamine regulated transcript (CART) positive neurons and CART mRNA by adrenalectomy and corticosterone replacement in hypothalamic nuclei of male rat brain. More recently, we have shown by CART immunohistochemistry that restraint and forced swim (FS) stress...
journal_title:Neuropeptides
pub_type: 杂志文章
doi:10.1016/j.npep.2006.02.001
更新日期:2006-06-01 00:00:00
abstract::The hypocretins/orexins (hcrts) are two recently described neuropeptides derived from the same precursor and expressed in a few thousand neurons in the perifornical area of the lateral hypothalamus, which project throughout the brain. The hypocretins bind to two G-protein coupled receptors with different selective aff...
journal_title:Neuropeptides
pub_type: 杂志文章,评审
doi:10.1054/npep.2002.0892
更新日期:2002-04-01 00:00:00
abstract::Down syndrome is the most common genetic disorder associated with mental retardation. Subjects and mice models for Down syndrome (such as Ts65Dn) show defects in the formation of neuronal networks in both the hippocampus and the cerebral cortex. The principal neurons display alterations in the morphology, density and ...
journal_title:Neuropeptides
pub_type: 杂志文章
doi:10.1016/j.npep.2011.10.002
更新日期:2012-02-01 00:00:00
abstract::Immunoreactive substance P (SPI) and somatostatin (SOMI) are found in spinal cord but their physiological roles remain speculative. Several classes of neuropeptides, including endogenous opioids and thyrotropin-releasing hormone (TRH), have been implicated in the pathogenesis or recovery from spinal cord injury. In th...
journal_title:Neuropeptides
pub_type: 杂志文章
doi:10.1016/0143-4179(85)90092-7
更新日期:1985-06-01 00:00:00
abstract::Neuropeptide Y (NPY) is known to bind to at least two types of receptors (Y1 & Y2). One type (Y2) is able to bind and undergo activation by both NPY and its C-terminal fragments with good potency while the other (Y1) requires the full length of NPY for good potency. For most NPY analogs that have been examined, potenc...
journal_title:Neuropeptides
pub_type: 杂志文章
doi:10.1016/0143-4179(90)90073-8
更新日期:1990-11-01 00:00:00
abstract::Two tachykinin peptides, substance P (SP) and hemokinin-1 (HK-1), and three transient receptor potential (TRP) channels, TRPV1, TRPA1 and TRPM8, are similarly localized in the spinal dorsal horn and dorsal root ganglion, suggesting that TRP channels may be related or modulated by these tachykinin peptides. Thus, to cl...
journal_title:Neuropeptides
pub_type: 杂志文章
doi:10.1016/j.npep.2009.10.002
更新日期:2010-02-01 00:00:00
abstract::In order to further test whether or not psychostimulant drugs activate CART peptide-containing cells in the nucleus accumbens, we examined the fraction of CART positive cells that co-immunostained for c-Fos after administration of saline or cocaine (10 and 25 mg/kg i.p.). There was about a 45% increase in the fraction...
journal_title:Neuropeptides
pub_type: 杂志文章
doi:10.1016/j.npep.2008.01.001
更新日期:2008-06-01 00:00:00
abstract::Lysine-vasopressin (LVP) at different concentrations (2 ng, 200 pg) was injected into the lateral ventricle and cisterna magna. The effect on hypothalamic self-stimulation was studied in rats. LVP (2 ng) decreased the self-stimulation rate after intracerebroventricular and cisternal application. Injection of 200 pg LV...
journal_title:Neuropeptides
pub_type: 杂志文章
doi:10.1016/s0143-4179(87)90119-3
更新日期:1987-11-01 00:00:00
abstract::6-Beta-fluoro-6-desoxy-oxymorphone (FOXY) is a fluorinated derivative of oxymorphone originally developed as a potential PET scanning ligand. Preliminary work (Rothman et al., Neuropeptides 4: 311-317, 1984) demonstrated that [3H]FOXY selectively labeled mu opioid binding sites with low levels of nonspecific binding. ...
journal_title:Neuropeptides
pub_type: 杂志文章
doi:10.1016/0143-4179(88)90020-0
更新日期:1988-01-01 00:00:00
abstract::Noradrenaline (NA) has been implicated in both increase and reduction of anxiety. Selective destruction of nerve endings of the locus coeruleus projections by DSP-4 has been shown to reduce active behaviour in novel situations by enhancing anxiety. In the present study, DSP-4 (50 mg/kg) treatment reduced locomotor act...
journal_title:Neuropeptides
pub_type: 杂志文章
doi:10.1054/npep.1999.0788
更新日期:2000-02-01 00:00:00
abstract::Pyroglutamyl diazomethyl ketone and N-benzyloxycarbonyl prolyl prolinal, specific inhibitors of pyroglutamyl peptidase I and prolyl endopeptidase respectively, were used to study the possible role of these enzymes in the regulation of thyrotropin releasing hormone turnover. In vitro thyrotropin releasing hormone relea...
journal_title:Neuropeptides
pub_type: 杂志文章
doi:10.1016/0143-4179(87)90010-2
更新日期:1987-05-01 00:00:00
abstract::In vivo microdialysis was employed to explore the effects of different selective non-peptides NK(1),NK(2) and NK(3) receptor antagonists on the corticotropin releasing factor (CRF)-induced release of acetylcholine (ACh) in the hippocampus of rats and guinea-pigs. In both species, the intracerebroventricular (i.c.v.) a...
journal_title:Neuropeptides
pub_type: 杂志文章
doi:10.1016/s0143-4179(03)00019-2
更新日期:2003-04-01 00:00:00
abstract::Possible positive reinforcing and aversive effects of intraperitoneally (ip) administered substance P (SP) and its C-terminal heptapeptide analog [pGlu5, MePhe8, Sar9]-SP5-11 (DIME-C7) were investigated in rats. The 'conditioned corral preference and avoidance procedure' was used for assessing positive and negative re...
journal_title:Neuropeptides
pub_type: 杂志文章
doi:10.1016/0143-4179(90)90037-y
更新日期:1990-12-01 00:00:00
abstract::PTHrP and its receptor PTHR1 are found in the CNS and peripheral nervous system. The presence of PTHrP mRNA has been detected in the superior cervical ganglion (SCG), but there are no data on the cellular distribution of PTHrP and PTHR1 in the SCG. Although it is known that ovarian activity and reproductive status inf...
journal_title:Neuropeptides
pub_type: 杂志文章
doi:10.1016/j.npep.2014.09.005
更新日期:2014-12-01 00:00:00
abstract::Omega Conotoxin is a peptide venom of the marine snail Conus geographus which has been postulated as a neural specific calcium channel blocker in several systems. In the present study in rat ileum strips omega-conotoxin GVIA specifically blocked neuronal responses evoked by electrical field stimulation in a dose-depen...
journal_title:Neuropeptides
pub_type: 杂志文章
doi:10.1016/0143-4179(89)90078-4
更新日期:1989-05-01 00:00:00
abstract::Cerebral amyloid-beta protein (Abeta) deposition and associated neuroinflammation and apoptosis are increasingly recognized as an important component leading to cognitive impairment in Alzheimer's disease (AD). Humanin (HN) and its derivative, S14G-HN (HNG), are best known for their ability to suppress neuronal death ...
journal_title:Neuropeptides
pub_type: 杂志文章,收录出版
doi:10.1016/j.npep.2008.08.004
更新日期:2008-10-01 00:00:00
abstract::Neuropeptide Y (NPY) -receptor subtypes were studied in the rat kidney in vivo by systemic administration of NPY, the two agonists [Leu(31), Pro(34)]NPY (Y1-receptor agonist) and NPY (13-36) (Y2-receptor agonist), or the Y1-receptor antagonist BIBP 3226. Effects on mean arterial blood pressure (MAP) and renal arterial...
journal_title:Neuropeptides
pub_type: 杂志文章
doi:10.1054/npep.1999.0755
更新日期:1999-08-01 00:00:00
abstract::We studied head-injured patients assessed after admission to the hospital according to the Glasgow coma scale (3-8 points). All of them underwent surgical procedure after neuroradiological examination. Due to therapeutic problems, some of these patients had lumbar puncture on the fourth day following surgery. The cont...
journal_title:Neuropeptides
pub_type: 杂志文章
doi:10.1016/s0143-4179(97)90012-3
更新日期:1997-02-01 00:00:00
abstract::Oxytocin, either intraperitoneally injected (200 ng/rat) or intracerebroventricularly infused (1 ng/rat in 4 microliters saline) 60 and 15 min respectively before testing, significantly increased the lordosis response to the mounting male of ovariectomized, estrogen/progesterone treated rats. The intracerebroventricul...
journal_title:Neuropeptides
pub_type: 杂志文章
doi:10.1016/0143-4179(85)90095-2
更新日期:1985-06-01 00:00:00
abstract::We investigated the effect of sulphated cholecystokinin octapeptide (CCK-8S) on free intracellular calcium concentration, phosphatidylinositol metabolism, and protein phosphorylation in C6 cells, a rat glioma cell line which was shown to express CCKB type receptors. Increase in [Ca2+]i by both influx across the cell m...
journal_title:Neuropeptides
pub_type: 杂志文章
doi:10.1016/0143-4179(95)90033-0
更新日期:1995-11-01 00:00:00