Burkholderia dilworthii sp. nov., isolated from Lebeckia ambigua root nodules.

Abstract:

:Three strains of Gram-stain-negative, rod-shaped bacteria were isolated from Lebeckia ambigua root nodules and authenticated on this host. Based on the 16S rRNA gene sequence phylogeny, they were shown to belong to the genus Burkholderia, with the representative strain WSM3556(T) being most closely related to Burkholderia caledonica LMG 23644(T) (98.70 % 16S rRNA gene sequence similarity) and Burkholderia rhynchosiae WSM3937(T) (98.50 %). Additionally, these strains formed a distinct group in phylogenetic trees of the housekeeping genes gyrB and recA. Chemotaxonomic data, including fatty acid profiles and analysis of respiratory quinones, supported the assignment of our strains to the genus Burkholderia. Results of DNA-DNA hybridizations, MALDI-TOF MS analysis and physiological and biochemical tests allowed genotypic and phenotypic differentiation of our strains from their nearest neighbour species. Therefore, these strains represent a novel species, for which the name Burkholderia dilworthii sp. nov. is proposed, with the type strain WSM3556(T) ( = LMG 27173(T) = HAMBI 3353(T)).

authors

De Meyer SE,Cnockaert M,Ardley JK,Van Wyk BE,Vandamme PA,Howieson JG

doi

10.1099/ijs.0.058602-0

subject

Has Abstract

pub_date

2014-04-01 00:00:00

pages

1090-1095

issue

Pt 4

eissn

1466-5026

issn

1466-5034

journal_volume

64

pub_type

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