Abstract：
:Cartilage loss is a leading cause of disability among adults and effective therapy remains elusive. Neonatal chondrocytes (NChons) are an attractive allogeneic cell source for cartilage repair, but their clinical translation has been hindered by scarce donor availability. Here we examine the potential for catalyzing cartilage tissue formation using a minimal number of NChons by co-culturing them with adipose-derived stem cells (ADSCs) in 3D hydrogels. Using three different co-culture models, we demonstrated that the effects of co-culture on cartilage tissue formation are dependent on the intercellular distance and cell distribution in 3D. Unexpectedly, increasing ADSC ratio in mixed co-culture led to increased synergy between NChons and ADSCs, and resulted in the formation of large neocartilage nodules. This work raises the potential of utilizing stem cells to catalyze tissue formation by neonatal chondrocytes via paracrine signaling, and highlights the importance of controlling cell distribution in 3D matrices to achieve optimal synergy.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Lai JH,Kajiyama G,Smith RL,Maloney W,Yang Fdoi
10.1038/srep03553subject
Has Abstractpub_date
2013-12-19 00:00:00pages
3553issn
2045-2322pii
srep03553journal_volume
3pub_type
杂志文章abstract：:A method for defect characterization is presented that allows to measure the activation energy, capture cross-section, and defect density in dielectric materials. This is exemplarily performed on aluminum oxide thin films deposited on hydrogen-terminated diamond. During the measurement, samples were illuminated using ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-69240-3
更新日期：2020-07-27 00:00:00
abstract：:Sublingual immunization is emerging as an alternative to nasal immunization and induction of mucosal IgA responses. Using Bacillus anthracis edema toxin (EdTx) as an adjuvant, we previously showed that innate responses triggered after sublingual immunization could limit generation of IgA responses. We tested whether c...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-36370-8
更新日期：2019-01-24 00:00:00
abstract：:The gliotransmitter glutamate released from astrocytes can modulate neuronal firing by activating neuronal N-methyl-D-aspartic acid (NMDA) receptors. This enables astrocytic glutamate(AG) to be involved in neuronal physiological and pathological functions. Based on empirical results and classical neuron-glial "tripart...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep32343
更新日期：2016-08-30 00:00:00
abstract：:Intracontinental biotic divisions across the vast Palaearctic region are not well-characterized. Past research has revealed patterns ranging from a lack of population structure to deep divergences along varied lines of separation. Here we compared biogeographic patterns of two Palaearctic shorebirds with different hab...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-54715-9
更新日期：2019-12-03 00:00:00
abstract：:We report on a highly efficient magneto-actuated cancer cell apoptosis method using a biaxial pulsed magnetic field configuration, which maximizes the induced magnetic torque. The light transmissivity dynamics show that the biaxial magnetic field configuration can actuate the magnetic nanoparticles with higher respons...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-11279-w
更新日期：2017-09-07 00:00:00
abstract：:Panax ginseng (GS) and Veratrum nigrum (VN) are representative of incompatible pairs in "eighteen antagonistic medicaments" that have been recorded in the Chinese medicinal literature for over 2,000 years. However, evidence linking interference effects with combination use is scare. Based on the estrogen-like effect o...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep26924
更新日期：2016-05-27 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-020-65654-1
更新日期：2020-05-26 00:00:00
abstract：:This work presents a new method for assisting in the identification process of missing persons in several contexts, such as enforced disappearances. We apply a Bayesian technique to incorporate non-genetic variables in the construction of prior information. In that way, we can learn from the already-solved cases of a ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-59841-3
更新日期：2020-03-19 00:00:00
abstract：:Algae-bacteria interaction is one of the main factors underlying the formation of harmful algal blooms (HABs). The aim of this study was to develop a genome-wide high-throughput screening method to identify HAB-influenced specific interactive bacterial metabolites using a comprehensive collection of gene-disrupted E. ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-67322-w
更新日期：2020-06-30 00:00:00
abstract：:The plant hormone abscisic acid (ABA) and the jasmonic acid related-compound 12-oxo-phytodienoic acid (OPDA) play crucial roles in seed development, dormancy, and germination. However, a lack of suitable techniques for visualising plant hormones has restricted the investigation of their biological mechanisms. In the p...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep42977
更新日期：2017-02-17 00:00:00
abstract：:Increasing evidence suggest that the glucose-lowering drug metformin exerts a valuable anti-senescence role. The ability of metformin to affect the biogenesis of selected microRNAs (miRNAs) was recently suggested. MicroRNA isoforms (isomiRs) are distinct variations of miRNA sequences, harboring addition or deletion of...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-78871-5
更新日期：2020-12-11 00:00:00
abstract：:The release of radon in active fault zones is a sustained radioactive pollution source of the atmospheric environment. The species, concentration and flux of radon emitted in soil gas in active fault zones in the Capital of China were investigated by in-situ field measurements. Two main species of radon discharging fr...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-35262-1
更新日期：2018-11-13 00:00:00
abstract：:Arctic river watersheds are important components of the global climate system and show an amplified response to climate change. Here, we characterize origins and transformations of dissolved organic matter (DOM) in five major Arctic rivers (Kolyma, Lena, Yenisei, Ob, Mackenzie) over 3 years with seasonal sampling peri...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-12729-1
更新日期：2017-10-19 00:00:00
abstract：:We developed a time-efficient semi-automated axon quantification method using freeware in human cranial nerve sections stained with paraphenylenediamine (PPD). It was used to analyze a total of 1238 facial and masseteric nerve biopsies. The technique was validated by comparing manual and semi-automated quantification ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-58917-4
更新日期：2020-02-06 00:00:00
abstract：:Hemodialysis patients are a special group of patients with higher mortality rates. Hemodialysis patients with vitamin D deficiency {plasma levels of 25-hydroxyvitamin D [25(OH)D] below 20 ng/mL} are associated with even higher mortality rates. The prognostic importance of vitamin D deficiency in hemodialysis patients ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-64359-9
更新日期：2020-05-05 00:00:00
abstract：:Bovine TB (bTB) is endemic in Irish cattle and has eluded eradication despite considerable expenditure, amid debate over the relative roles of badgers and cattle in disease transmission. Using a comprehensive dataset from Northern Ireland (>10,000 km(2); 29,513 cattle herds), we investigated interactions between host ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep13062
更新日期：2015-08-17 00:00:00
abstract：:Mucociliary clearance is a biomechanical mechanism of airway protection. It consists of the active transport along the bronchial tree of the mucus, a fluid propelled by the coordinated beating of a myriad of cilia on the epithelial surface of the respiratory tract. The physics of mucus transport is poorly understood b...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-20882-4
更新日期：2018-02-05 00:00:00
abstract：:We provide an explicit model for a spin-1/2 quasi-particle, based on the superposition of plasmon excitations in a quantum plasmas with intrinsic orbital angular momentum. Such quasi-particle solutions can show remarkable similarities with single electrons moving in vacuum: they have spin-1/2, a finite rest mass, and ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-26196-9
更新日期：2018-05-18 00:00:00
abstract：:Periconceptional supplementation with folic acid reduces the occurrence of neural tube defects (NTDs). The association between maternal abnormalities in homocysteine metabolism (e.g., hyperhomocysteinaemia, folate deficiency and low vitamin B12) and the risk of NTDs-affected pregnancies has been widely evaluated in re...
journal_title：Scientific reports
pub_type： 杂志文章,meta分析
doi：10.1038/srep08510
更新日期：2015-03-02 00:00:00
abstract：:The behavior of clogging has a close relationship with the biofilm attached on inner surface of the pipeline in a drip irrigation system using reclaimed water. Therefore, inhibiting biofilm growth is the key to completely addressing the clogging problem. Water shear forces play a vital role in the formation, developme...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-63898-5
更新日期：2020-04-24 00:00:00
abstract：:Human respiratory syncytial virus (RSV) A ON1 genotype, first detected in 2010 in Ontario, Canada, has been documented in 21 countries to date. This study investigated persistence and transmission dynamics of ON1 by grouping 406 randomly selected RSV-positive specimens submitted to Public Health Ontario from August 20...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep14268
更新日期：2015-09-30 00:00:00
abstract：:Natural and anthropogenic stressors can cause phase shifts from coral-dominated to algal-dominated states. In the Caribbean, over-fishing of large herbivorous fish and disease among the long-spined urchin, Diadema, have facilitated algal growth on degraded reefs. We found that diminutive species of urchin and parrotfi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep39670
更新日期：2017-01-05 00:00:00
abstract：:Hair cortisol concentration (HCC) is a promising measure of long-term hypothalamus-pituitary-adrenal (HPA) axis activity. Previous research has suggested an association between HCC and psychological variables, and initial studies of inter-individual variance in HCC have implicated genetic factors. However, whether HCC...
journal_title：Scientific reports
pub_type： 临床试验,杂志文章
doi：10.1038/s41598-017-11852-3
更新日期：2017-11-10 00:00:00
abstract：:The placenta forms a maternal-fetal junction that supports many physiological functions such as the supply of nutrition and exchange of gases and wastes. Establishing an in vitro culture model of human and non-human primate trophoblast stem/progenitor cells is important for investigating the process of early placental...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-63602-7
更新日期：2020-04-22 00:00:00
abstract：:Changes in immune and coagulation systems and possible viral spread through the blood-brain barrier have been described in SARS-CoV-2 infection. In this study, we evaluated the possible retinal involvement and ocular findings in severe COVID-19 pneumonia patients. A cross-sectional study was conducted on 46 patients a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-74446-6
更新日期：2020-10-15 00:00:00
abstract：:We used Mendelian randomization to estimate the causal effects of the liver enzymes, alanine aminotransferase (ALT), alkaline phosphatase (ALP) and gamma glutamyltransferase (GGT), on diabetes and cardiovascular disease, using genetic variants predicting these liver enzymes at genome wide significance applied to exten...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep38813
更新日期：2016-12-20 00:00:00
abstract：:Bone marrow and adipose tissue represent the two most commonly exploited sources of adult mesenchymal stem cells for musculoskeletal applications. Unfortunately the sampling of bone marrow and fat tissue is invasive and does not always lead to a sufficient number of cells. The present study describes a novel sampling ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-00803-7
更新日期：2017-04-06 00:00:00
abstract：:Understanding the rhizodeposited carbon (C) dynamics of winter wheat (Triticum aestivum L.), is crucial for soil fertility and C sequestration. Pot-grown winter wheat was pulse labelled with 14CO2 at the key growth stages. 14C in the shoots, roots and soil was measured at 5 or 2 days after 14C-labelling (DAL 5/2) at e...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-21547-y
更新日期：2018-02-16 00:00:00
abstract：:Period circadian clock (Per) genes Per1 and Per2 have essential roles in circadian oscillation. In this study, we identified a new role of Per1-Per2 cooperation, and its mechanism, using our new experimental methods. Under constant light conditions, the period length of Per1 and Per2 knockout mice depended on the copy...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep32769
更新日期：2016-09-09 00:00:00
abstract：:A cerebral aneurysm occurs as a result of a weakened blood vessel, which allows blood to flow into a sac or a ballooned section. Recent advancement shows that a new device, 'flow-diverter', can divert blood flow away from the aneurysm sac. People found that a flow-diverter based on thin film nitinol (TFN), works very ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep23698
更新日期：2016-03-24 00:00:00
