Abstract:
:Cholinergic signaling plays an important role in regulating the growth and regeneration of axons in the nervous system. The α7 nicotinic receptor (α7) can drive synaptic development and plasticity in the hippocampus. Here, we show that activation of α7 significantly reduces axon growth in hippocampal neurons by coupling to G protein-regulated inducer of neurite outgrowth 1 (Gprin1), which targets it to the growth cone. Knockdown of Gprin1 expression using RNAi is found sufficient to abolish the localization and calcium signaling of α7 at the growth cone. In addition, an α7/Gprin1 interaction appears intimately linked to a Gαo, growth-associated protein 43, and CDC42 cytoskeletal regulatory pathway within the developing axon. These findings demonstrate that α7 regulates axon growth in hippocampal neurons, thereby likely contributing to synaptic formation in the developing brain.
journal_name
J Neurochemjournal_title
Journal of neurochemistryauthors
Nordman JC,Phillips WS,Kodama N,Clark SG,Del Negro CA,Kabbani Ndoi
10.1111/jnc.12641subject
Has Abstractpub_date
2014-05-01 00:00:00pages
649-62issue
4eissn
0022-3042issn
1471-4159journal_volume
129pub_type
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