Suppression of VEGF expression through interruption of the HIF‑1α and Akt signaling cascade modulates the anti‑angiogenic activity of DAPK in ovarian carcinoma cells.

Abstract:

:Death-associated protein kinase (DAPK) plays an important role in apoptosis regulation and has been shown to maintain antitumor and metastasis suppressor properties. In the present study, we investigated whether DAPK overexpression may mediate vascular endothelial growth factor (VEGF)/hypoxia-inducible factor-1α (HIF-1α) expression and angiogenic activity in the human carcinoma cell model system. VEGF plays a pivotal role in tumor angiogenesis and tumorigenesis. We found that DAPK significantly downregulated VEGF-induced endothelial cell proliferation, migration and tube formation as well as VEGF receptor-2 (VEGFR-2) phosphorylation in vitro. In addition, DAPK exhibited potent anti-angiogenic activity and clearly decreased the levels of VEGF and HIF-1α expression, a key regulator for angiogenesis. Notably, our results strongly indicated that DAPK can disturb VEGFR-2 transcriptional activity by inhibiting VEGFR-2 phosphorylation through the PI3K/Akt signaling cascade. Collectively, our study identified a novel function of DAPK in regulating cellular VEGF/HIF-1α activity during tumorigenesis, which may act together with its anti-angiogenic function to inhibit tumor progression.

journal_name

Oncol Rep

journal_title

Oncology reports

authors

Park ST,Kim BR,Park SH,Lee JH,Lee EJ,Lee SH,Rho SB

doi

10.3892/or.2013.2928

subject

Has Abstract

pub_date

2014-02-01 00:00:00

pages

1021-9

issue

2

eissn

1021-335X

issn

1791-2431

journal_volume

31

pub_type

杂志文章
  • The Das-1 immunostain is useful for discriminating metastatic colon adenocarcinoma from cholangiocarcinoma and hepatocellular carcinoma.

    abstract::The distinction between cholangiocarcinoma, hepatocellular carcinoma and liver metastasis is important but at times difficult solely on microscopic appearances. The Das-1 immunostain exhibits specificity for colonic epithelium. However, its staining of cholangiocarcinomas and hepatocellular carcinomas has not been ext...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:

    authors: Zimmerman RL,Das KM,Fogt F,Burke M,Murakata LA

    更新日期:2002-11-01 00:00:00

  • Effects of the histone deacetylase inhibitor valproic acid on the sensitivity of anaplastic thyroid cancer cell lines to imatinib.

    abstract::New therapeutic approaches are mandatory for anaplastic thyroid cancer. We investigated the ability of a new combined treatment using valproic acid (VPA), the only clinically available histone deacetylase inhibitor, and the tyrosine-kinase inhibitor imatinib mesylate to control the cell growth of anaplastic thyroid ca...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:

    authors: Catalano MG,Pugliese M,Poli R,Bosco O,Bertieri R,Fortunati N,Boccuzzi G

    更新日期:2009-02-01 00:00:00

  • Fascin is involved in cancer cell invasion and is regulated by stromal factors.

    abstract::The tumor microenvironment plays an important role in cancer growth, invasion and metastasis. The stroma surrounding a tumor is known to contain a variety of factors that can increase angiogenesis, cancer growth and tumor progression. The aim of the present study was to determine the role of fascin in cancer growth an...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2018.6847

    authors: Zhang X,Cho IH,Park JH,Lee MK,Hwang YS

    更新日期:2019-01-01 00:00:00

  • DNA vaccines based on chimeric potyvirus-like particles carrying HPV16 E7 peptide (aa 44-60).

    abstract::Vaccine strategies for the treatment of human papillomavirus-induced cervical cancer are based mainly on the human papillomavirus 16 E7 (HPV16 E7) oncoprotein. The immunogenicity of the E7 gene has been enhanced by its fusion to many different genes. Here, we linked a short sequence coding for the E7 peptide (aa 44-60...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:

    authors: Pokorna D,Cerovska N,Smahel M,Moravec T,Ludvikova V,Mackova J,Synkova H,Duskova M,Hozak P,Veleminsky J

    更新日期:2005-10-01 00:00:00

  • Carboplatin/etoposide induces remission of metastasised malignant peripheral nerve tumours (malignant schwannoma) refractory to first-line therapy.

    abstract::Malignant peripheral nerve tumours (MPNT), e.g. malignant schwannoma, represent a relatively rare tumour entity which is usually regarded as a member of the group of soft-tissue sarcomas and treated accordingly. For chemotherapeutic approaches in metastasised disease, ifosfamide and doxorubicin have been identified as...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:

    authors: Steins MB,Serve H,Zühlsdorf M,Senninger N,Semik M,Berdel WE

    更新日期:2002-05-01 00:00:00

  • Sanguinarine-induced apoptosis in lung adenocarcinoma cells is dependent on reactive oxygen species production and endoplasmic reticulum stress.

    abstract::Sanguinarine (SAN), an alkaloid isolated from plants of the Papaveraceae family, is a compound with multiple biological activities. In the present study, we explored the anticancer properties of SAN in lung cancer using the human lung adenocarcinoma cell line SPC-A1. Our results revealed that SAN inhibited SPC-A1 cell...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2015.4054

    authors: Gu S,Yang XC,Xiang XY,Wu Y,Zhang Y,Yan XY,Xue YN,Sun LK,Shao GG

    更新日期:2015-08-01 00:00:00

  • Alterations of the K-ras and p53 genes in Tamoxifen-associated endometrial carcinoma.

    abstract::To better understand the molecular mechanisms of carcinogenesis induced in uterine endometrium by therapeutic anti-estrogenic Tamoxifen (TAM) exposure, 27 uterine tumors (4 benign endometrial polyps and 23 carcinomas) associated with TAM exposure were analyzed for the presence and spectrum of p53 and K-ras mutations. ...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:

    authors: Fujiwara K,Enomoto T,Fujita M,Kanda T,Fujii S,Ito K,Wakasa K,Ishiko O,Ueda M,Yamaguchi S,Kimura T,Umesaki N

    更新日期:2008-05-01 00:00:00

  • β‑carotene reverses tobacco smoke‑induced gastric EMT via Notch pathway in vivo.

    abstract::Tobacco smoke is one of the serious risk factors of gastric cancer. Epithelial‑mesenchymal transition (EMT) has been shown to be associated with the initiation and carcinogenesis of gastric cancer. The role of Notch pathway in regulating tobacco smoke-induced EMT has not been investigated. β‑carotene, a carotenoid pre...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2018.6246

    authors: Lu L,Chen J,Li M,Tang L,Wu R,Jin L,Liang Z

    更新日期:2018-04-01 00:00:00

  • miR-194 is a negative regulator of GEF-H1 pathway in melanoma.

    abstract::The incidence and associated mortality of melanoma continues to increase worldwide. At present, there is no curative therapy for advanced stage of melanoma. It is necessary to find new indicators of prognosis and therapeutic targets. Increasing evidence shows that miRNA can provide potential candidate biomarkers for m...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2016.5020

    authors: Guo B,Hui Q,Zhang Y,Chang P,Tao K

    更新日期:2016-10-01 00:00:00

  • Decreased miRNA-148a is associated with lymph node metastasis and poor clinical outcomes and functions as a suppressor of tumor metastasis in non-small cell lung cancer.

    abstract::Non-small cell lung cancer (NSCLC) is the leading cause of cancer-related death worldwide, and only 15% of lung cancer patients live more than 5 years. microRNAs (miRNAs) are endogenously expressed non-coding RNAs, and dysregulation of miRNAs is a common feature in human cancers including lung cancer. In this study, w...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2013.2611

    authors: Chen Y,Min L,Zhang X,Hu S,Wang B,Liu W,Wang R,Gu X,Shen W,Lv H,Zou J,Chen Y,Xu X,Chen L

    更新日期:2013-10-01 00:00:00

  • Curcumin induces apoptosis through FAS and FADD, in caspase-3-dependent and -independent pathways in the N18 mouse-rat hybrid retina ganglion cells.

    abstract::Curcumin, a naturally occurring yellow pigment isolated from turmeric, is a well known antioxidant with broad spectrum of anti-tumor activities against many human cancer cells. In this study, curcumin-induced cytotoxic effect of mouse-rat hybrid retina ganglion cells (N18) were investigated. For determining cell viabi...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or_00000411

    authors: Lu HF,Lai KC,Hsu SC,Lin HJ,Yang MD,Chen YL,Fan MJ,Yang JS,Cheng PY,Kuo CL,Chung JG

    更新日期:2009-07-01 00:00:00

  • Development of a rapid genotyping method for single nucleotide polymorphisms and its application in cancer studies.

    abstract::Single nucleotide polymorphisms (SNPs) in cancer-related genes can act as low risk genetic factors for the development of this disease. SNPs have also been shown to influence the efficacy and toxicity of various cytotoxic agents used in the treatment of cancer. Progress in these important areas of cancer research reli...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:

    authors: Grieu F,Joseph D,Norman P,Iacopetta B

    更新日期:2004-02-01 00:00:00

  • Emodin inhibits epithelial to mesenchymal transition in epithelial ovarian cancer cells by regulation of GSK-3β/β-catenin/ZEB1 signaling pathway.

    abstract::Emodin (EMO) has been shown to possess pleiotropic anticancer capabilities in many types of cancer, including epithelial ovarian cancer (EOC). Inhibitory efficacy of EMO on EOC invasion and migration was previously observed, however, the underlying mechanisms have not been completely elucidated. The present study is a...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2016.4591

    authors: Hu C,Dong T,Li R,Lu J,Wei X,Liu P

    更新日期:2016-04-01 00:00:00

  • Modifying effect of tuna orbital oil rich in docosahexaenoic acid and vitamin D3 on azoxymethane-induced colonic aberrant crypt foci in rats.

    abstract::The modifying effect of dietary tuna (Thunnus thynnus orientalis) orbital oil rich in docosahexaenoic acid (DHA) and vitamin D3 (VD3) on the development of azoxymethane (AOM)-induced colonic aberrant crypt foci (ACF) was investigated in male F344 rats. Animals were given three weekly subcutaneous injections of AOM (15...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.7.5.1069

    authors: Kohno H,Yamaguchi N,Ohdoi C,Nakajima S,Odashima S,Tanaka T

    更新日期:2000-09-01 00:00:00

  • Transcriptional silencing of ETS-1 abrogates epithelial-mesenchymal transition resulting in reduced motility of pancreatic cancer cells.

    abstract::v-ets erythroblastosis virus E26 oncogene homolog 1 (ETS-1) plays crucial roles in a spectrum of malignancies. ETS-1 has gained attention in cancer research for its importance in cell migration, invasion and proliferation. In the present study, we focused on the effect of ETS-1 on epithelial-mesenchymal transition (EM...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2014.3613

    authors: Li C,Wang Z,Chen Y,Zhou M,Zhang H,Chen R,Shi F,Wang C,Rui Z

    更新日期:2015-02-01 00:00:00

  • Houttuynia cordata Thunb extract induces apoptosis through mitochondrial-dependent pathway in HT-29 human colon adenocarcinoma cells.

    abstract::The Houttuynia cordata Thunb (HCT) extract has been used as a traditional Chinese herb medicine and as well as an effective drug for treating allergic inflammation for thousands of years. In this study, we investigated the anti-cancer activity of HCT and its molecular mechanisms in the human colon adenocarcinoma cell ...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or_00000535

    authors: Tang YJ,Yang JS,Lin CF,Shyu WC,Tsuzuki M,Lu CC,Chen YF,Lai KC

    更新日期:2009-11-01 00:00:00

  • Autocrine interferon-γ may affect malignant behavior and sensitivity to tamoxifen of MCF-7 via estrogen receptor β subtype.

    abstract::Mitogenic actions of estrogens are mediated by two distinct estrogen receptors (ERs), which are critical in the progression and therapeutic response of breast cancer. ER expression is a dynamic phenomenon that is regulated by numerous factors, including cytokines, in the tumor microenvironment. Recently, studies have ...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2015.4294

    authors: Niu XL,Wang Y,Yao Z,Duan H,Li Z,Liu W,Zhang H,Deng WM

    更新日期:2015-12-01 00:00:00

  • miR-519d-mediated downregulation of STAT3 suppresses breast cancer progression.

    abstract::MicroRNAs (miRNAs) play important roles in the the gene regulation of carcinogenesis including breast cancer. miR‑519d has been studied in various types of cancer, but its role in breast cancer remains unclear. In the present study, we investigated the expression and biological function of miR-519d in breast cancer. U...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2015.4160

    authors: Deng X,Zhao Y,Wang B

    更新日期:2015-10-01 00:00:00

  • HSPA9 overexpression inhibits apoptin-induced apoptosis in the HepG2 cell line.

    abstract::Apoptin, a small protein derived from chicken anemia virus, possesses the capacity to specifically kill tumor cells while leaving normal cells intact. Previous studies have indicated that the subcellular localization of apoptin appears to be crucial for this tumor-selective activity. Apoptin resides in the cytoplasm o...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2013.2399

    authors: Peng C,Yang P,Cui Y,He M,Liang L,Di Y

    更新日期:2013-06-01 00:00:00

  • In vivo antitumor effect of ascorbic acid, lysine, proline and green tea extract on human colon cancer cell HCT 116 xenografts in nude mice: evaluation of tumor growth and immunohistochemistry.

    abstract::Colorectal cancer is the second most deadly cancer in the United States. When diagnosed early, current treatments bring a limited success; however, once metastasis occurs, radiation and chemotherapy are generally ineffective. Structural changes in the ECM are necessary for cell migration during tissue remodeling. Matr...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:

    authors: Roomi MW,Ivanov V,Kalinovsky T,Niedzwiecki A,Rath M

    更新日期:2005-03-01 00:00:00

  • Gene expression profiling of HL-60 cells following knockdown of nucleostemin using DNA microarrays.

    abstract::Nucleostemin (NS) plays an important role in tumorigenesis and progression. Most studies consider that NS plays its role through combining with p53 and inhibiting it, however our previous studies revealed that NS could also function without the existence of p53. To date, few studies have focused on the p53-independent...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2014.3240

    authors: Sun X,Jia Y,Wei Y,Liu S,Yue B

    更新日期:2014-08-01 00:00:00

  • IL22 furthers malignant transformation of rat mesenchymal stem cells, possibly in association with IL22RA1/STAT3 signaling.

    abstract::Mesenchymal stem cells (MSCs) hold great promise as potential therapies for tumors through the delivery of various anticancer agents. However, exogenous tissue‑derived MSCs, such as those of bone marrow, have exhibited a tendency for malignant transformation in the tumor microenvironment. This issue remains controvers...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2019.7007

    authors: Cui X,Jing X,Yi Q,Xiang Z,Tian J,Tan B,Zhu J

    更新日期:2019-04-01 00:00:00

  • Chemotherapy under cachectic conditions and the possibility of cachexia-controlled chemotherapy.

    abstract::We studied the role of chemotherapy in cancer cachexia, which is well known to lower QOL and responsiveness to chemotherapy. In BALB/c mice we used two clones derived from the murine colon 26 adenocarcinoma cell line; clone 5, a non-cachexigenic tumor and clone 20, a cachexigenic tumor, in which IL-6 mRNA was selectiv...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:

    authors: Takahashi Y,Yasumoto K,Mai M

    更新日期:2005-07-01 00:00:00

  • Tetrandrine inhibits hepatocellular carcinoma cell growth through the caspase pathway and G2/M phase.

    abstract::Activation of p53-independent pathways plays an important role in phytochemical-induced apoptosis and is considered to be a crucial factor in the invasion and metastasis of cancer. Previous studies have shown that combined effects of Stephania tetrandra with medicinal herbs exhibit beneficial effects in cancer patient...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2013.2352

    authors: Yu VW,Ho WS

    更新日期:2013-06-01 00:00:00

  • Prognostic impact of δ-like ligand 4 and Notch1 in acute myeloid leukemia.

    abstract::Notch signaling plays a critical role in embryonic vascular development and tumor angiogenesis. The present study was conducted to investigate the prognostic role of the angiogenesis-related Notch ligand and the receptor in acute myeloid leukemia (AML) and assess whether their expre...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2012.1943

    authors: Zhang J,Ma D,Ye J,Zang S,Lu F,Yang M,Qu X,Sun X,Ji C

    更新日期:2012-10-01 00:00:00

  • Schisandrin B inhibits cell proliferation and induces apoptosis in human cholangiocarcinoma cells.

    abstract::Cholangiocarcinoma (CCA) is the second most common hepatic cancer with high resistance to current chemotherapies and extremely poor prognosis. The present study aimed to examine the effects of schisandrin B (Sch B) on CCA cells both in vitro and in vivo and to examine its underlying mechanism. We found that Sch B inhi...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2016.4992

    authors: Yang X,Wang S,Mu Y,Zheng Y

    更新日期:2016-10-01 00:00:00

  • Association between mitochondrial DNA 4,977 bp deletion and NAD(P)H:quinone oxidoreductase 1 C609T polymorphism in human breast tissues.

    abstract::The NAD(P)H:quinone oxidoreductase 1 (NQO1) enzyme is implicated in protection against oxidative stress and carcinogenesis. NQO1 C609T genetic polymorphism was reported to be associated with an increased risk for cancers, including breast cancer. However, there is still lack of evidence whether higher oxidative stress...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or_00000337

    authors: Tseng LM,Yin PH,Tsai YF,Chi CW,Wu CW,Lee LM,Lee HC

    更新日期:2009-05-01 00:00:00

  • Chemokines mediate mesenchymal stem cell migration toward gliomas in vitro.

    abstract::Previous studies have demonstrated the tremendous tropism of mesenchymal stem cells (MSCs) for malignant gliomas, making these cells a potential vehicle for delivery of therapeutic genes to disseminated glioma cells. However, the mechanisms underlying the tropism of MSCs for gliomas remain poorly defined. It has been ...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or_00000796

    authors: Xu F,Shi J,Yu B,Ni W,Wu X,Gu Z

    更新日期:2010-06-01 00:00:00

  • Silencing MARCH1 suppresses proliferation, migration and invasion of ovarian cancer SKOV3 cells via downregulation of NF-κB and Wnt/β-catenin pathways.

    abstract::Membrane-associated RING-CH (MARCH) belongs to the family of RING-CH type E3 ubiquitin ligases. MARCH1 ubiquitinates and downregulates MHC class II expression in APCs and targets major players of the immune system. However, the role of MARCH1 in ovarian cancer has not been elucidated. The present study investigated th...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2016.5076

    authors: Meng Y,Hu J,Chen Y,Yu T,Hu L

    更新日期:2016-11-01 00:00:00

  • RNAi-mediated knockdown of the c-jun gene sensitizes radioresistant human nasopharyngeal carcinoma cell line CNE-2R to radiation.

    abstract::This study aimed to investigate the effect of RNA interference (RNAi)-mediated downregulation of the expression of the c-jun gene (a proto-oncogene) on the radiosensitivity of a radioresistant human nasopharyngeal carcinoma cell line (CNE-2R) and to validate its potential as an anticancer target. A lentiviral vector w...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2014.3692

    authors: Guo SY,Zhu XD,Ge LY,Qu S,Li L,Su F,Guo Y

    更新日期:2015-03-01 00:00:00