Abstract:
:Advances in sequencing technologies have enabled the identification of mutations acquired by bacterial pathogens during infection. However, it remains unclear whether adaptive mutations fix in the population or lead to pathogen diversification within the patient. Here we study the genotypic diversity of Burkholderia dolosa within individuals with cystic fibrosis by resequencing individual colonies and whole populations from single sputum samples. We find extensive intrasample diversity, suggesting that mutations rarely fix in a patient's pathogen population--instead, diversifying lineages coexist for many years. Under strong selection, multiple adaptive mutations arise, but none of these sweep to fixation, generating lasting allele diversity that provides a recorded signature of past selection. Genes involved in outer-membrane components, iron scavenging and antibiotic resistance all showed this signature of within-patient selection. These results offer a general and rapid approach for identifying the selective pressures acting on a pathogen in individual patients based on single clinical samples.
journal_name
Nat Genetjournal_title
Nature geneticsauthors
Lieberman TD,Flett KB,Yelin I,Martin TR,McAdam AJ,Priebe GP,Kishony Rdoi
10.1038/ng.2848subject
Has Abstractpub_date
2014-01-01 00:00:00pages
82-7issue
1eissn
1061-4036issn
1546-1718pii
ng.2848journal_volume
46pub_type
杂志文章相关文献
NATURE GENETICS文献大全abstract::We report germline missense mutations in ETV6 segregating with the dominant transmission of thrombocytopenia and hematologic malignancy in three unrelated kindreds, defining a new hereditary syndrome featuring thrombocytopenia with susceptibility to diverse hematologic neoplasms. Two variants, p.Arg369Gln and p.Arg399...
journal_title:Nature genetics
pub_type: 杂志文章
doi:10.1038/ng.3177
更新日期:2015-02-01 00:00:00
abstract::Whipworms are common soil-transmitted helminths that cause debilitating chronic infections in man. These nematodes are only distantly related to Caenorhabditis elegans and have evolved to occupy an unusual niche, tunneling through epithelial cells of the large intestine. We report here the whole-genome sequences of th...
journal_title:Nature genetics
pub_type: 杂志文章
doi:10.1038/ng.3010
更新日期:2014-07-01 00:00:00
abstract::To identify new susceptibility loci for psoriasis, we undertook a genome-wide association study of 594,224 SNPs in 2,622 individuals with psoriasis and 5,667 controls. We identified associations at eight previously unreported genomic loci. Seven loci harbored genes with recognized immune functions (IL28RA, REL, IFIH1,...
journal_title:Nature genetics
pub_type: 杂志文章
doi:10.1038/ng.694
更新日期:2010-11-01 00:00:00
abstract::Recent studies have demonstrated that a locus at 11p15.5 confers susceptibility to insulin dependent diabetes mellitus (IDDM). This locus has been shown to lie within a 19 kb region. We present a detailed sequence comparison of the predominant haplotypes found in this region in a population of French Caucasian IDDM pa...
journal_title:Nature genetics
pub_type: 杂志文章
doi:10.1038/ng0793-305
更新日期:1993-07-01 00:00:00
abstract::MicroRNAs (miRNAs) are key regulators of gene expression in animals and plants. Studies in a variety of model organisms show that miRNAs modulate developmental processes. To our knowledge, the only hereditary condition known to be caused by a miRNA is a form of adult-onset non-syndromic deafness, and no miRNA mutation...
journal_title:Nature genetics
pub_type: 杂志文章
doi:10.1038/ng.915
更新日期:2011-09-04 00:00:00
abstract::Immunotherapy for metastatic colorectal cancer is effective only for mismatch repair-deficient tumors with high microsatellite instability that demonstrate immune infiltration, suggesting that tumor cells can determine their immune microenvironment. To understand this cross-talk, we analyzed the transcriptome of 91,10...
journal_title:Nature genetics
pub_type: 杂志文章,多中心研究
doi:10.1038/s41588-020-0636-z
更新日期:2020-06-01 00:00:00
abstract::Cas9 is commonly introduced into cell lines to enable CRISPR-Cas9-mediated genome editing. Here, we studied the genetic and transcriptional consequences of Cas9 expression itself. Gene expression profiling of 165 pairs of human cancer cell lines and their Cas9-expressing derivatives revealed upregulation of the p53 pa...
journal_title:Nature genetics
pub_type: 杂志文章
doi:10.1038/s41588-020-0623-4
更新日期:2020-07-01 00:00:00
abstract::Consistent but indirect evidence has implicated genetic factors in smoking behavior. We report meta-analyses of several smoking phenotypes within cohorts of the Tobacco and Genetics Consortium (n = 74,053). We also partnered with the European Network of Genetic and Genomic Epidemiology (ENGAGE) and Oxford-GlaxoSmithKl...
journal_title:Nature genetics
pub_type: 杂志文章,meta分析
doi:10.1038/ng.571
更新日期:2010-05-01 00:00:00
abstract::The Human Genome Project and its spin-offs are making it increasingly feasible to determine the genetic basis of complex traits using genome-wide association studies. The statistical challenge of analyzing such studies stems from the severe multiple-comparison problem resulting from the analysis of thousands of SNPs. ...
journal_title:Nature genetics
pub_type: 杂志文章
doi:10.1038/ng1582
更新日期:2005-07-01 00:00:00
abstract::Legionella pneumophila, the causative agent of Legionnaires' disease, replicates as an intracellular parasite of amoebae and persists in the environment as a free-living microbe. Here we have analyzed the complete genome sequences of L. pneumophila Paris (3,503,610 bp, 3,077 genes), an endemic strain that is predomina...
journal_title:Nature genetics
pub_type: 杂志文章
doi:10.1038/ng1447
更新日期:2004-11-01 00:00:00
abstract::A single microarray can provide information on the expression of tens of thousands of genes. The amount of information generated by a microarray-based experiment is sufficiently large that no single study can be expected to mine each nugget of scientific information. As a consequence, the scale and complexity of micro...
journal_title:Nature genetics
pub_type: 杂志文章,评审
doi:10.1038/ng1028
更新日期:2002-12-01 00:00:00
abstract::Genomic imprinting is an epigenetic modification that results in expression from only one of the two parental copies of a gene. Differences in methylation between the two parental chromosomes are often observed at or near imprinted genes. Beckwith-Wiedemann syndrome (BWS), which predisposes to cancer and excessive gro...
journal_title:Nature genetics
pub_type: 杂志文章
doi:10.1038/ng988
更新日期:2002-11-01 00:00:00
abstract::Systemic lupus erythematosus (SLE) is a highly prevalent human autoimmune diseases that causes progressive glomerulonephritis, arthritis and an erythematoid rash. Mice deficient in deoxyribonuclease I (Dnase1) develop an SLE-like syndrome. Here we describe two patients with a heterozygous nonsense mutation in exon 2 o...
journal_title:Nature genetics
pub_type: 杂志文章
doi:10.1038/91070
更新日期:2001-08-01 00:00:00
abstract::The early development of blood vessels consists of two phases, vasculogenesis and angiogenesis, which involve distinct and also overlapping molecular regulators, but the intracellular signal transduction pathways involved in these processes have not been well defined. We disrupted Map3k3 (also known as Mekk3), which e...
journal_title:Nature genetics
pub_type: 杂志文章
doi:10.1038/73550
更新日期:2000-03-01 00:00:00
abstract::Novel approaches to the structural and functional analysis of mammalian chromosomes would be possible if the gross structure of the chromosomes in living cells could be engineered. Controlled modifications can be engineered by conventional targeting techniques based on homologous recombination. Large but uncontrolled ...
journal_title:Nature genetics
pub_type: 杂志文章
doi:10.1038/ng1292-283
更新日期:1992-12-01 00:00:00
abstract::Type 1 diabetes (T1D) is a common autoimmune disorder that arises from the action of multiple genetic and environmental risk factors. We report the findings of a genome-wide association study of T1D, combined in a meta-analysis with two previously published studies. The total sample set included 7,514 cases and 9,045 ...
journal_title:Nature genetics
pub_type: 杂志文章
doi:10.1038/ng.381
更新日期:2009-06-01 00:00:00
abstract::Transcriptional silencing by CpG island methylation is a prevalent mechanism of tumor-suppressor gene suppression in cancers. Genetic experiments have defined the importance of the DNA methyltransferase Dnmt1 for the maintenance of methylation in mouse cells and its role in neoplasia. In human bladder cancer cells, se...
journal_title:Nature genetics
pub_type: 杂志文章
doi:10.1038/ng1068
更新日期:2003-01-01 00:00:00
abstract::In metastatic cancer, the degree of heterogeneity of the tumor microenvironment (TME) and its molecular underpinnings remain largely unstudied. To characterize the tumor-immune interface at baseline and during neoadjuvant chemotherapy (NACT) in high-grade serous ovarian cancer (HGSOC), we performed immunogenomic analy...
journal_title:Nature genetics
pub_type: 杂志文章
doi:10.1038/s41588-020-0630-5
更新日期:2020-06-01 00:00:00
abstract::Micro RNAs are a large family of noncoding RNAs of 21-22 nucleotides whose functions are generally unknown. Here a large subset of Drosophila micro RNAs is shown to be perfectly complementary to several classes of sequence motif previously demonstrated to mediate negative post-transcriptional regulation. These finding...
journal_title:Nature genetics
pub_type: 杂志文章
doi:10.1038/ng865
更新日期:2002-04-01 00:00:00
abstract::R-spondins are a recently characterized small family of growth factors. Here we show that human R-spondin1 (RSPO1) is the gene disrupted in a recessive syndrome characterized by XX sex reversal, palmoplantar hyperkeratosis and predisposition to squamous cell carcinoma of the skin. Our data show, for the first time, th...
journal_title:Nature genetics
pub_type: 杂志文章
doi:10.1038/ng1907
更新日期:2006-11-01 00:00:00
abstract::The eighth annual Human Genome Variation Meeting was held in September 2006 in the Hong Kong Special Administrative Region, China. The meeting highlighted recent advances in characterization of genetic variation, including genome-wide association studies and structural variation. ...
journal_title:Nature genetics
pub_type:
doi:10.1038/ng0207-153
更新日期:2007-02-01 00:00:00
abstract::In embryonic stem cells (ESCs), developmental gene promoters are characterized by their bivalent chromatin state, with simultaneous modification by MLL2 and Polycomb complexes. Although essential for embryogenesis, bivalency is functionally not well understood. Here, we show that MLL2 plays a central role in ESC genom...
journal_title:Nature genetics
pub_type: 杂志文章
doi:10.1038/s41588-018-0218-5
更新日期:2018-10-01 00:00:00
abstract::Pituitary adenoma is one of the most common intracranial neoplasms, and its genetic basis remains largely unknown. To identify genetic susceptibility loci for sporadic pituitary adenoma, we performed a three-stage genome-wide association study (GWAS) in the Han Chinese population. We first analyzed genome-wide SNP dat...
journal_title:Nature genetics
pub_type: 杂志文章
doi:10.1038/ng.3322
更新日期:2015-07-01 00:00:00
abstract::Limb-girdle muscular dystrophy (LGMD) is a clinically and genetically heterogeneous group of myopathies, including autosomal dominant and recessive forms. To date, two autosomal dominant forms have been recognized: LGMD1A, linked to chromosome 5q, and LGMD1B, associated with cardiac defects and linked to chromosome 1q...
journal_title:Nature genetics
pub_type: 杂志文章
doi:10.1038/ng0498-365
更新日期:1998-04-01 00:00:00
abstract::Autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED) is the only described systemic autoimmune disease with established monogenic background, and the first autoimmune disorder localized outside the major histocompatibility complex (MHC) region. The primary biochemical defect in APECED is unknown. We...
journal_title:Nature genetics
pub_type: 杂志文章
doi:10.1038/ng1297-399
更新日期:1997-12-01 00:00:00
abstract::Genome-wide association studies (GWAS) have not consistently detected replicable genetic risk factors for ischemic stroke, potentially due to etiological heterogeneity of this trait. We performed GWAS of ischemic stroke and a major ischemic stroke subtype (large artery atherosclerosis, LAA) using 1,162 ischemic stroke...
journal_title:Nature genetics
pub_type: 杂志文章,meta分析
doi:10.1038/ng.2397
更新日期:2012-10-01 00:00:00
abstract::Although several lung cancer susceptibility loci have been identified, much of the heritability for lung cancer remains unexplained. Here 14,803 cases and 12,262 controls of European descent were genotyped on the OncoArray and combined with existing data for an aggregated genome-wide association study (GWAS) analysis ...
journal_title:Nature genetics
pub_type: 杂志文章,meta分析
doi:10.1038/ng.3892
更新日期:2017-07-01 00:00:00
abstract::Human synovial sarcomas contain a recurrent and specific chromosomal translocation t(X;18)(p11.2;q11.2). By screening a synovial sarcoma cDNA library with a yeast artificial chromosome spanning the X chromosome breakpoint, we have identified a hybrid transcript that contains 5' sequences (designated SYT) mapping to ch...
journal_title:Nature genetics
pub_type: 杂志文章
doi:10.1038/ng0894-502
更新日期:1994-08-01 00:00:00
abstract::After domestication, during a process of widespread range extension, barley adapted to a broad spectrum of agricultural environments. To explore how the barley genome responded to the environmental challenges it encountered, we sequenced the exomes of a collection of 267 georeferenced landraces and wild accessions. A ...
journal_title:Nature genetics
pub_type: 杂志文章
doi:10.1038/ng.3612
更新日期:2016-09-01 00:00:00
abstract::Plant imprinted genes show parent-of-origin expression in seed endosperm, but little is known about the nature of parental imprints in gametes before fertilization. We show here that single differentially methylated regions (DMRs) correlate with allele-specific expression of two maternally expressed genes in the seed ...
journal_title:Nature genetics
pub_type: 杂志文章
doi:10.1038/ng1828
更新日期:2006-08-01 00:00:00